首页 | 本学科首页   官方微博 | 高级检索  
相似文献
 共查询到16条相似文献,搜索用时 125 毫秒
1.
激素对百合植株再生的影响   总被引:11,自引:0,他引:11  
刘明志  林雪艳 《广西植物》2002,22(2):167-170
本研究设计了 1 5种不同配比的激素组合研究 2 ,4 -D和 6 -BA对百合鳞片叶器官发生和体细胞胚胎发生的影响。结果表明 :在 MS培养基上 ,BA可诱导外植体直接分化不定芽 ,其中 1 .0~ 2 .0 mg/L BA诱导不定芽的分化频率最高 ,6 .0 mg/L BA抑制不定芽分化 ;2 ,4 -D可诱导直接体细胞胚胎发生 ,其中 4 .0 mg/L2 ,4 -D诱导体细胞胚胎发生的频率最高 ,而 6 .0 mg/L 2 ,4 -D诱导体细胞胚胎发生的频率降低 ;当培养基中同时含有 BA和 2 ,4 -D时 ,既出现不定芽 ,又出现体细胞胚。当再生苗移入无激素的 MS培养基和含有 1 .0mg/L和 2 .0 mg/L IAA的 MS培养基上时 ,只有无激素的 MS培养基有利于根的形成。此外 ,鳞片叶的大小和外植体的接种方向也影响外植体分化  相似文献   

2.
2,4-D诱导的花生体细胞胚发生的组织学研究   总被引:14,自引:0,他引:14  
花生成熟胚胚叶在MS附加 2 0mg/L 2 ,4_D的培养基上诱导 2 0d后 ,转移至无激素培养基MS0 继续培养 ,可获高频体细胞胚发生。组织学观察表明 ,体细胞胚起源于胚叶上表皮及表皮下数层细胞 ,这些细胞脱分化形成细胞质浓厚、细胞核大的胚性细胞团 ,胚性细胞团继续分裂形成体细胞胚。体细胞胚的发育过程经历球形胚、心形胚、鱼雷胚、子叶胚四个时期  相似文献   

3.
不同激素对花生离体分化的影响   总被引:8,自引:0,他引:8  
对TDZ和2,4-D等激素在花生成熟胚外植体分化中的影响进行了研究.结果表明,花生成熟胚3~5 d龄实生苗的幼叶和胚轴在低浓度TDZ的诱导下,可分化产生高频不定芽和少量体细胞胚,转到无激素MS培养基或MS BA 0.5 mg/L NAA 0.4 mg/L的培养基后形成丛生苗.丛生苗分离后转入含1/2 MS(大量元素) IBA 0.4 mg/L的培养基中诱导生根,可形成完整的再生植株.幼叶分化率高于胚轴,但胚轴分化成苗速度快.无菌水浸泡16~24 h的胚轴在5~ 30 mg/L 2,4-D的诱导下,分化产生低频不定芽;而胚叶则产生高频体细胞胚,但畸形较严重.  相似文献   

4.
花生成熟胚胚叶在佃附加20mg/L 2,4-D的培养基上诱导20d后,转移至无激素培养基MS0继续培养,可获高频体细胞胚发生。组织学观察表明,体细胞胚起源于胚叶上表皮及表皮下数层细胞,这些细胞脱分化形成细胞质浓厚、细胞核大的胚性细胞团,胚性细胞团继续分裂形成体细胞胚。体细胞胚的发育过程经历球形胚、心形胚、鱼雷胚、子叶胚四个时期。  相似文献   

5.
胡杨器官和体胚发生方式的植株再生   总被引:1,自引:0,他引:1  
目的:为以胡杨为亲本的体细胞杂交育种奠定基础。方法:以胡杨苗叶片为外植体,通过器官和体胚两种不同发生方式建立了离体再生体系。结果:附加0.75mg/L BA、0.5mg/L NAA基本培养基及3w暗培养是愈伤组织诱导的最佳条件;附加0.25mg/L BA和0.1mg/L NAA的基本培养基上不定芽的诱导率最高;1/2大量元素的MS培养基附加0.1mg/l NAA、0.05mg/L和1.5%蔗糖对不定芽生根效果最好;诱导并筛选出的胚性愈伤组织在附加了0.5mg/L BA、0.5mg/L NAA的基本培养基上诱导获得大量胚状体,干化处理后大部分能经子叶胚期萌发成苗。结论:外植体的采集周期和培养条件影响胡杨离体叶片的形态发生途径。  相似文献   

6.
酿酒葡萄"梅尔诺"再生系统建立的研究   总被引:6,自引:0,他引:6  
以酿酒葡萄“梅尔诺”离体胚珠、叶柄为材料.通过控制激素水平、光照和温度等,对建立再生体系的器官发生途径和体胚发生途径进行了研究。结果表明,体胚的诱导和不定芽的再生与基本培养基、叶柄的着生部位、生长调节物质种类和浓度等因素有关。由“梅尔诺”的胚珠愈伤组织再生出体细胞胚的最佳培养基配方为CPSE培养基(CP287 BA 0.2mg/L NOA 1.0mg/L),体细胞胚再生率可达47.50%。“梅尔诺”体细胞胚在CPSE培养基上100%萌发为芽状,将其切断置于培养基MS TDZ 4.0mg/L上可直接诱导出绿色不定芽,再生率为52.25%;同时在培养基MS TDZ2.0mg/L上获得了“梅尔诺”离体叶柄再生不定芽.再生率为62.42%.二者再生的不定芽的最他增殖培养基为MS BA0.5mg/L。“梅尔诺”体细胞胚的萌发芽在WPM培养基中能很好的生根及成苗,并建立了单芽茎段微繁体系。  相似文献   

7.
鱼腥草体细胞胚胎发生和植株再生   总被引:1,自引:0,他引:1  
王莲  袁艺 《激光生物学报》2007,16(6):722-726
目的:利用鱼腥草的叶片和叶柄为材料,进行体细胞胚胎诱导及植株再生研究。方法:运用正交设计试验,考察在改良的MS固体培养基上添加不同种类、不同浓度的植物生长物质组合及其配比对鱼腥草愈伤组织诱导、体细胞胚胎发生及植株再生的影响。结果:鱼腥草无菌苗叶片在含有2,4-D 1.0 mg/L 6-BA 0.5 mg/L的改良MS培养基上能诱导出胚性愈伤组织;胚性愈伤组织在含有6-BA 1.0 mg/L的改良的MS培养基上诱导体细胞胚的发生;叶柄在含有6-BA 1.0 mg/L改良MS培养基上直接产生体细胞胚。体细胞胚在改良的MS NAA0.1 mg/L 6-BA 1.0 mg/L的培养基上能够快速繁殖,形成大量不定芽,在不加任何激素的MS培养基上就可以萌发出不定根,发育为成完整植株,在MS IBA 1.0 mg/L的固体培养基上能够形成大量的根。结论:建立了鱼腥草体细胞胚胎发生及植株再生的体系。  相似文献   

8.
以何首乌茎尖、茎段为外植体,经体细胞胚发生途径,进行胚性愈伤组织诱导、体细胞胚的诱导、植株再生的研究.并采用临时压片法对体细胞胚的发育过程进行观察.结果表明愈伤组织诱导最适培养基为Ms+6-BA 2.0 mg/L+NAA 0.5 mg/L,体细胞胚诱导最适培养基为MS+6-BA 1.0 mg/L+NAA 0.2 mg/L.将产生的体细胞胚首先接种于MS基本培养基使其充分发育后转入MS+6-BA 2.0 mg/L培养基中诱导出芽,出芽率高于直接采用Ms+6-BA 2.0 mg/L培养基诱导.体细胞胚的发育过程是首先在愈伤组织表面形成许多瘤状突起即胚性细胞团,胚性细胞团继续发育成球形胚、盾形胚,球形胚、盾形胚成熟后发育成植株.  相似文献   

9.
白皮松成熟胚的离体培养研究   总被引:5,自引:0,他引:5  
以白皮松成熟胚为外植体诱导再生小植株.试验结果表明,成熟胚不定芽诱导以MS培养基最佳,附加0.294 mg/L的NAA和3.56 mg/L的6-BA时,诱导率接近100%;MS培养基附加NAA(0.05 mg/L)时,平均增殖系数可达6.3以上.不定芽增殖率最大值(10)出现在SH培养基上,此时NAA浓度为0.05 mg/L、6-BA浓度为4 mg/L.MS培养基中加入适量活性炭和GA3能促进不定芽生长,随着活性炭和GA3浓度的增加,有效嫩梢(≥2cm和≥4 cm)的比率显著增高;当活性炭和GA3浓度过高时(分别超过2.75 g/L和4.1 mg/L),不定芽的伸长与生长受到抑制.在离体培养条件下,以种胚为外植体获得了无根苗.  相似文献   

10.
栓皮栎体细胞胚胎发生的细胞组织学观察   总被引:1,自引:0,他引:1  
以栓皮栎未成熟合子胚为外植体,在添加0.25mg/L 2,4-D和0.5mg/L 6-BA的MS培养基上6周可诱导产生2种类型的胚性愈伤组织,一种表面具光泽、白色;另一种表面光滑湿润具光泽,色泽淡黄或无色透明。组织切片表明,胚性愈伤组织的细胞体积小,细胞核大,细胞质浓,细胞排列紧密;非胚性愈伤组织细胞的体积大,细胞核小,细胞质稀薄。胚性细胞团培养在不含激素的培养基上可诱导产生体细胞胚。体细胞胚直接起源于胚性细胞团表皮或近表皮的单细胞,经历与合子胚相似的球形胚、心形胚、鱼雷胚和子叶胚发育阶段。所有发育时期的体细胞胚的胚轴、子叶均产生次生体胚,它们起源于细胞质较浓的表皮单细胞。  相似文献   

11.
Immature zygotic embryos were cultured on Murashige and Skoog's medium (MS) supplemented with various combinations of 2,4-dichlorophenoxyacetic acid (2,4-D), naphthaleneacetic acid (NAA), benzyladenine (BA) and zeatin or with various concentrations of 2,4-D alone. The maximum number (8 per embryo) of adventitious buds formed from cotyledons of heart stage embryos cultured on MS medium with 1 mg dm−3 BA and 0.01 mg dm−3 NAA. The adventitious buds originated from procambial strands of immature embryo cotyledons and then developed into adventitious bud primordia within 20 d. Adventitious buds transferred to hormone free MS medium grew into shoots, but did not produce plantlets because the shoots failed to root. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   

12.
Callusing, caulogenesis, in vitro flowering and somatic embryogenesis were induced from the base of leaflets derived from mature embryos of peanut (Arachis hypogaea) by altering the hormonal composition of the Murashige and Skoog's (MS) basal medium. A combination of 4 mg/l alpha napththaleneacetic acid (NAA) and 5 mg/l 6-benylaminopurine (BAP) was optimum for inducing caulogenic buds. The caulogenic buds proliferated in medium with 3 mg/l BAP. Differentiation of these buds to shoots was achieved in MS basal medium with 0.5 mg/l each of BAP and kinetin (KN). Shoot buds and flower buds were produced when caulogenic buds were cultured on medium containing 1 mg/l BAP and 1 mg/l KN, prior to elongation. Clonally propagated plantlets derived from axillary buds elongated, formed roots and were grown to maturity in soil. Embryogenic mass formation was induced from the leaf base in the presence of 20 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D). Somatic embryos developed upon reducing 2,4-D to 3 mg/l.  相似文献   

13.
体外培养对于植物的快速繁殖是非常有效的。和其它一些松果类硬木植物一样,火炬松的体外培养成功率却一直很低。本工作研究了不同的基本培养基和低温条件对于火炬松J-56, S-1003, and E-440等三个品系的成熟合子胚形成愈伤组织、分化出芽、成苗的影响。在不同的基本培养基条件下芽分化的程度差异很大。合子胚经过9-12周培养,开始分化,形成具有器官发生的愈伤组织(Fig.2a)。分化后3周,开始诱导出芽(Fig.2b),芽的生长快慢不同(Fig.2c,d)。同一个愈伤组织上会生出几个芽来(Fig.2e)。在添加有IBA和BA的TE培养基上芽生长最快(Fig.1)。低温条件持续 15天,能增加芽的数量和分化的程度(Table1)。上述培养基中增加GA3时表明,GA3对于根的诱导有决定性的作用。将98株再生苗转移到特殊的混合土壤上;成活了75株苗(Fig.2f)。以这三种火炬松的再生苗尖为材料制备DNA。用20个引物进行RAPD分析,结果表明:这三种火炬松苗的扩增产物是相同的(Fig.2g,h&i)。这说明:用愈伤组织克隆植株的过程中没有引起植物遗传变异。  相似文献   

14.
Cotyledon explants of ginseng (Panax ginseng C. A. Meyer) produced somatic embryos directly on medium without growth regulators, with 89% of the explants forming somatic embryos. Cytokinin treatment greatly suppressed somatic embryo formation but stimulated the direct formation of adventitious buds. BAP treatment was more effective than the kinetin treatment for adventitious bud formation. Auxin (0.05 mg/l IBA) in combination with cytokinin enhanced adventitious bud formation, with the highest frequency, 40%, at 0.05 mg/l IBA and 5 mg/l BAP. Adventitious buds were mainly formed near the distal portion of the cotyledons, while somatic embryos were formed near the proximal excised margins. Shoots were developed from adventitious buds after transfer to MS medium with 10 mg/l GA3. Root formation from the shoots was obtained after the shoots were transferred to half-strength MS medium with auxin (IAA). When the plants derived from adventitious buds were transferred to greenhouse soil, 36% were successfully acclimatized. Received: 7 November 1997 / Revision received: 12 January 1998 / Accepted: 7 February 1998  相似文献   

15.
An efficient and reproducible procedure is established for the plant regeneration from hypocotyl explants and hypocotyl-or stem-derived calli in Astragalus melilotoides. High frequency somatic embryo formation (98.3%) occurred direct on hypocotyls on Murashige and Skoog (MS) medium supplemented with 2.69 µM NAA and 4.44 µM BA within 5 weeks. Three types of calli were induced from the hypocotyl and stem segments on MS medium containing 9.05 µM 2,4-D and 2.22–4.44 µM BA. Both somatic embryos and adventitious buds were initiated from hypocotyl-derived calli while only adventitious buds were formed from stem-derived calli in MS medium supplemented with 2.69 µM NAA and 4.44–8.89 µM BA. Somatic embryos or adventitious buds developed into plantlets following being cultured for 3 weeks on MS medium without any growth regulators or with 14.78 µM IBA, respectively. All the regenerated plants were normal with respect to morphology and growth characters, and produced fertile seeds after planting in soil.  相似文献   

16.
A reproducible protocol for somatic embryogenesis was established for mango ginger (Curcuma amada Roxb.)—an important horticultural aromatic rhizomatous plant. Embryogenic callus induction was obtained from leaf sheath explants of in vitro raised plants on Murashige and Skoog (MS) agar medium containing 2.0 mg/L 2,4-dichlorophenoxyacetic acid and 0.5 mg/L 6-benzyladenine (BA). Embryogenic callus proliferation, somatic embryo (SE) formation and subsequent plantlet conversion occurred under optimal culture conditions. The effects of MS medium strength, sucrose and BA on SE formation were also evaluated. Half strength MS liquid medium necessary for SE formation and optimal sucrose concentration was found to be 3.0 %. BA at 0.3 mg/L produced the highest number (84.71 %) of SEs from leaf sheath explants. Secondary somatic embryos originated from primary somatic embryos on the same medium supplemented with 0.4–0.6 mg/L BA. Stereo microscopic and scanning electron microscopic observation revealed that the globular and torpedo shaped somatic embryos resulted in suspension culture during development. Mature somatic embryos germinated readily and developed into normal plantlets after 3 weeks on half strength MS basal agar medium under dark condition. Well rooted plantlets were successfully acclimatized at the survival rate of 70 %.  相似文献   

设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号