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1.
汞对玉米幼苗膜脂过氧化及体内保护系统的影响   总被引:10,自引:0,他引:10  
随着处理HgCl2浓度的升高,细胞膜脂质过氧化水平升高,细胞膜透性增大,CAT活性降低,SOD、POD活性升高,组织可溶性蛋白质含量升高。  相似文献   

2.
阳军  孙华保 《蛇志》1998,10(4):17-19
目的观察辛伐他丁对冠心病高血脂症患者血清脂质的影响。方法冠心病高血脂症病人50例,均有血脂(TC、TG、HDL-C、LDL-C或4项中任何一项)异常。服用辛伐他丁,5~20mg,每日1次,晚间顿服,疗程8周。服药前后晨起空腹查血脂以作自身对照。结果对高TC下降有效率达91.5%,对高LDL-C下降有效率达82.5%,对高TG下降有效率达76.2%,对低HDL-C增高有效率为72.2%。其中对TC、LDL-C治疗8周有极显著意义(P<0.01),,对TG下降、HDL-C升高8周后有显著意义(P<0.05)。治疗期间不良反应轻微。结论辛伐他丁可作为冠心病患者减少CHD事件发病率及住院率的理想药物。  相似文献   

3.
甘薯叶柄原生质体有效植株再生   总被引:4,自引:0,他引:4  
将甘薯(Ipomoeabatatas(L.)Lam.)‘元气’和‘白星’(‘WhiteStar’)的叶柄原生质体培养在含有0.05mg·L-12,4D和0.5mg·L-1KT的改良MS液体培养基中,3~4d后细胞开始分裂。培养8~9周后,将直径达1~2mm的愈伤组织转移到添加0.05~0.2mg·L-12,4D和0~0.5mg·L-1KT或添加0.5~2.0mg·L-1NAA和1.0~3.0mg·L-1BAP的MS固体增殖培养基上使其增殖。转移3~5周后,将愈伤组织再转移到MS基本培养基或转移到添加2.0~3.0mg·L-1BAP的MS培养基上。当进一步转移到MS基本培养基上后,从愈伤组织或从愈伤组织形成的不定根上再生出植株。‘元气’植株再生率高达60.0%,WhiteStar高达43.4%。  相似文献   

4.
T—2毒素对心肌细胞电生理特性的影响及硒的保护作用   总被引:3,自引:0,他引:3  
本实验在培养的Wistar大鼠乳鼠心肌细胞上观察了T-2毒素对膜电位活动的影响及硒的保护作用。结果表明,T-2毒素(0.1,0.5,5.0mg/L)使心肌细胞动作电位幅值(APA)、超射(OS)、阈电位(TP)、最大舒张电位(MDP)及最大除极速率(Vmax)显著降低。使复极化时程(APD10、APD50、APK90)延长;动作电位发放频率(APF)受到明显抑制。提示T-2毒素能抑制Ca^2+、K  相似文献   

5.
项圈藻的生长及其主要营养成分的研究   总被引:6,自引:0,他引:6  
项圈藻是程海湖中一优势种.在实验条件下,其生长的最适温度为25℃,光照强度为6000Lx,光照时间为24h昼夜连续光照,NO-3N为10.00mg·L-1,PO-P为1.00mg·L-1,CO32-C为150.00mg·L-1,Fe3+为1.00mg·L-1,PH为9.0.其最大增长率为0.817,最短增倍时间为0.85d.其粗蛋白、粗脂肪、总糖和灰份的含量分别占干重的61.25、5.30、20.34和8.25%;氨基酸含量为52.63%.  相似文献   

6.
种子引发提高小麦抗渗透胁迫能力的效应   总被引:5,自引:1,他引:4  
种子经渗透势-0.8MPa或-1.2MPaPEG-6000处理后,抗旱性不同小麦(TriticumaestivumL.)“晋麦33”和“84G6”种子萌发率均有提高,种子电解质外渗率明显降低,种子萌发适应渗透胁迫能力提高,种子可溶蛋白质含量、PAL和POD活性显著增加,“晋麦33”种子CAT活性增加,两品种幼苗PAL、POD和CAT活性也均有提高,但“84G6”种子CAT活性下降。  相似文献   

7.
体外培养大鼠星形细胞的缺糖缺氧性损伤及药物的保护   总被引:4,自引:1,他引:3  
本实验用大鼠星形细胞体外培养模型,观察了细胞在不同条件下乳酸脱氨酶(LDH)的漏出。发现当去掉葡萄糖后,细胞缺氧5和7h后LDH漏出明显增加,5b为59.7±25.3U/mg蛋白(对照组24.7±16.3,P<0.05),7h为68.3±89U/mg蛋白(对照组39.9±212,P<0.01)。用3-氧-甲基-[1- ̄3H]-D-葡萄糖摄取法测量细胞体积,结果显示缺糖缺氧5h后细胞明显肿胀,从对照组的4.1±1.2增加到8.1±3.2μl/mg蛋白(P<0.01)。丹参有效成分764-3在0.5至50μmol/L时能减少缺糖缺氧细胞LDH漏出;在50μmol/L时能减小缺糖缺氧细胞的体积。谷氨酸受体拮抗剂DNQX(6,7-dinitroquinoxaline-2,3dione)50μmol/L能减轻星形细胞肿胀和LDH漏出。  相似文献   

8.
6—BA诱导的带正电荷的葡萄叶过氧化物酶   总被引:7,自引:0,他引:7  
河岸葡萄叶的带正电荷过氧化物酶可受6-BA诱导,但盐,H2O2或Fe^2++H2O2均使叶片COPD活性明显下降,而高浓度的无机盐明显刺激纯COPD活性增加。在以愈创木酚为底物时CPOD最适pH为4.60-5.75,对H2O2的表面Vmax和Km值分别为110U/mg蛋白和1.15mmol/L。  相似文献   

9.
作物细胞耐旱保护酶系统对外磁场的反应   总被引:12,自引:0,他引:12  
作物细胞的耐旱保护酶由过氧化物酶(POD) 、过氧化氢酶(CAT) 和超氧化物歧化酶(SOD) 组成。对小麦种子施加0.1T 的磁场处理使其萌发时细胞中POD 活性提高,幼苗根系和叶片细胞中的POD 活性也发生了变化,叶片的POD 同工酶谱中多出了两个酶带。使用蛋白质合成抑制剂和转录抑制剂发现,POD 活性提高的原因是磁场处理促进了POD 合成的翻译过程。干旱胁迫下,经磁场处理的幼苗叶片细胞中的POD、CAT 和SOD 活性均比对照高,膜脂过氧化产物丙二醛( MDA) 含量比对照低,表明保护酶系统的功能有所增强。  相似文献   

10.
Cu对中国林蛙蝌蚪的急性毒性   总被引:19,自引:1,他引:18  
试验测定了不同温度条件下Cu2+对中国林蛙蝌蚪的急性毒性.结果表明,在水温为9~13℃、18℃和24℃条件下,Cu2+对10d龄中国林蛙蝌蚪96h的LC50分别为5.01、3.80和2.99mg·L-1,致死阈浓度分别为4.0、2.5和1.6mg·L-1,而在96h内的无可观察效应浓度分别为2.5、1.6和1.0mg·L-1.18℃条件下Cu2+对20d龄中国林蛙蝌蚪的96hLC50为2.17mg·L-1.  相似文献   

11.
《Neuron》2023,111(6):767-786
  相似文献   

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14.
ABSTRACT

We fabricated uniform straw-like cell architecture with central lumen using a suture thread within 1 h. The architecture consisting of cancer cells and mature adipocyte was used for cell–cell communication assay, although mature adipocyte could not form spontaneous multi-cellular spheroids. Using the system, it is possible to investigate three-dimensional cell–cell communication as an alternative to animal experiments.  相似文献   

15.
Programmed cell death in cell cultures   总被引:21,自引:0,他引:21  
In plants most instances of programmed cell death (PCD) occur in a number of related, or neighbouring, cells in specific tissues. However, recent research with plant cell cultures has demonstrated that PCD can be induced in single cells. The uniformity, accessibility and reduced complexity of cell cultures make them ideal research tools to investigate the regulation of PCD in plants. PCD has now been induced in cell cultures from a wide range of species including many of the so-called model species. We will discuss the establishment of cell cultures, the fractionation of single cells and isolation of protoplasts, and consider the characteristic features of PCD in cultured cells. We will review the wide range of methods to induce cell death in cell cultures ranging from abiotic stress, absence of survival signals, manipulation of signal pathway intermediates, through the induction of defence-related PCD and developmentally induced cell death.  相似文献   

16.
Summary A transitional cell carcinoma cell line, COLO 232, was derived from a primary urinary bladder tumor in a Caucasian male. In culture, COLO 232 retained distinct uroepithelial phenotypic traits and produced both carcinoembryonic antigen and adrenocorticotropic hormone. COLO 232 had a chromosome mode of 58 and retained the X and Y chromosomes. Ten marker chromosomes were identified. COLO 232 will be of value for biochemical and immunological studies. Presented in part at the 28th Annual Meeting of the Tissue Culture Association, June 7, 1977. This work was supported by Grant No. CA 15018 awarded by the National Cancer Institute, DHEW, and the Mary B. and L. H. Marshall Fund.  相似文献   

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A survey of studies on reconstructions of animal and plant cells which apply a new physical method--electrostimulated fusion, is presented. Effects of different factors of the medium on the efficiency of electrofusion is discussed. A detailed account is given of the authors' studies on zygotes reconstruction by combined methods of microsurgery and electrostimulated cell fusion. Advantages of the latter as compared to the widely distributed methods of fusion by polyethylenglycol and Sendai virus are considered. This physical method can play an important role in the progress of cellular engineering.  相似文献   

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