正常足月儿畸变产物耳声发射(DPOAEs)特性分析

研究提出正常足月儿畸变产物耳声发射（ＤＰＯＡＥｓ） 特性分析如下：１ ． ＤＰＯＡＥｓ 反应强度曲线,ＤＰ 图显示两个反应峰（ｆ２ ＝ １ ．６ 和５ ．０ ｋＨｚ） 和一个反应谷（ｆ２ ＝ ３ ．１ ～４ ．０ ｋＨｚ） ;２． ＤＰＯＡＥ 本底噪声及其特性,ｆ２ ＝ １．０ ｋＨｚ 其测试频率点（２ｆ１ －ｆ２） 本底噪声最高（Ｐ＜ ０ ．０５） ,ｆ２ ＝ ３ ．１ ,４．０ 和５ ．０ｋＨｚ ,测试频率点（２ｆ１ －ｆ２） 本底噪声较低（Ｐ＜ ０．０５） ;除ｆ２ ＝ ４．０ ｋＨｚ 外,ＤＰＯＡＥ 本底噪声与其反应强度均未呈现直线相关特性;３ ． ＤＰＯＡＥ ＳＮＲ 特性,ｆ２ ＝ １．０ ｋＨｚ 其ＳＮＲ 最小（Ｐ＝ ０．０００） ,ｆ２ ＝２ ．０ ｋＨｚ 其ＳＮＲ 最大（Ｐ０ ．００３） ;４． ＤＰＯＡＥ ＳＮＲ 和ＴＥＯＡＥ ＳＮＲ 相关特性,除１．０ ｋＨｚ 频段外,其余频段其二者间均有着非常显著的直线相关特性。     

几种氨基酸─铜（Ⅱ）配合物的超氧化物歧化酶活性

用四氮唑蓝光化学还原法对所合成的ＫＣｕ（ＩＤＡ）（Ｓｅｒ）·２Ｈ２Ｏ、ＫＣｕ（ＩＤＡ）（Ａｌａ）·Ｈ２Ｏ、Ｃｕ（ＩＤＡ）（ｅｎ）、ＫＣｕ（ＩＤＡ）（Ｇｌｙ）·Ｈ２Ｏ和Ｃｕ（ＩＤＡ）·２Ｈ２Ｏ（ＩＤＡ＝Ｎ（羧基甲基）－甘氨酸,Ｓｅｒ＝丝氨酸,Ａｌａ＝丙氨酸,ｅｎ＝乙二胺,Ｇｌｙ＝甘氨酸）等５种氨基酸─铜（Ⅱ）配合物进行了活性测定,发现它们均具有天然超氧化物歧化酶活性,其活性依次为０．３４、０．４５、０．５０、０．５４、０．７２Ｃｕμｍｏｌ·Ｌ－１。     

名贵月季品种微繁及工艺研究

研究以名贵月季品种“落霞”、“墨红”、“和平”、“金背大红”等为试材,以ＭＳ基本培养基附加不同浓度的６－ＢＡ、ＮＡＡ,以诱导腋芽增殖为微繁途径。结果表明：无菌外植体的建立培养基为ＭＳ＋６－ＢＡＯ．５－２＋ＮＡＡＯ．０５－０．１＋Ｖｃ１００;芽增殖培养基为ＭＳ十ＢＡ１＋ＮＡＡ０．０５,在此培养基上繁殖系数为５．５;生根培养基为ＭＳ＋ＩＡＡ１＋ＩＢＡ１＋ＮＡＡ０．１－肌醇,生根率在９５％左右。生根苗移栽成活率在８５％左右。同时对繁殖工艺流程、成本、商品苗产率等问题进行了讨论,给出在繁殖系数为Ｆ、生根数为ｂ时,有效繁殖系数ｆ＝Ｆ—ｂ,试管苗实际增殖倍数Ｎｎ＝（Ｆ—ｂ）（ｎ－１）·Ｆ、生根苗数Ｒ１－ｎ＝ｆ－１;当生根率为ｒ１,移栽成活率为ｒ２,则商品苗产率为Ｃ＝ｒ１·ｒ２·Ｒｎ＝ｒ１·ｒ２·ｂ（ｆ－１）／（ｆ－１）     

N_2和NH_4~＋培养下粪产碱菌固氮酶铁蛋白圆二色谱及热力学特性比较

Ｎ２和ＮＨ培养下粪产碱菌固氮酶铁蛋白（分别为Ａｆ２和Ａｆ＊２）的氧化态和还原态的ＣＤ谱及ＭＣＤ谱存在明显差异。还原态Ａｆ２与Ａｆ＊２在２１０ｎｍ附近的ＭＣＤ谱完全不同,但它们的氧化态ＭＣＤ谱相同。热力学测定结果表明．在２９８°Ｋ,１．０１３×１０５Ｐａ下,Ａｆ２与ＭｇＡＴＰ饱合络合时的培（△Ｈ°）变化为－２７．０ｋＪ／ｍｏｌ,自由能（△Ｇ°）变化则为－２０．８ｋＪ／ｍｏｌ．熵（△Ｓ°）变４也为－２０．６Ｊｍｏｌ－１Ｋ－１。而Ａｆ＊２与ＭｇＡＴＰ饱合络合时的烂变化为－４４．３５ｋＪ／ｍｏｌ,自由能变化为一２０．４ｋＪ／ｍｏｌ,熵变化为－７９．９Ｊｍｏｌ－１Ｋ－１。Ａｆ２与ＭｇＡＴＰ络合后再与Ｎ２培养的钼铁蛋白反应为吸热反应,而Ａｆ＊２与ＭｇＡＴＰ络合后再与ＮＨ培养的钼铁蛋白反应为放热反应。     

青海海北地区高山嵩草草甸植物群落生物量动态及能量分配

对青海海北地区高山草甸主要植物群落小嵩草（Ｋｏｂｒｅｓｉａｐｙｇｍａｅａ）草甸、矮嵩草（Ｋ．ｈｕｍｉｌｉｓ）草甸、藏嵩草（Ｋ．ｔｉｂｅｔｉｃａ）沼泽化草甸地上生物量动态和能量分配的研究结果表明,不同植物群落年地上净生产量及其年际动态和主要植物类群生物量季节动态具明显的差异,其生物量季节动态可由如下模型表示：Ｗｉ＝Ｋｉ／（１＋ｅｘｐ（Ａｉ－Ｂｉｔ））植物群落地上、地下生物量的垂直分布呈典型的金字塔和倒金字塔模式。小嵩草草甸、矮嵩草草甸和藏嵩草沼泽化草甸的地上净生产量依次为３６８．４ｇｍ－２ａ－１、４１８．５ｇｍ－２ａ－１和５１８．４ｇｍ－２ａ－１,所固定的太阳能值依次为６６５５．１６ｋＪｍ－２ａ－１、７６１０．０９ｋＪｍ－２ａ－１、９４８８．７７ｋＪｍ－２ａ－１。光能利用率分别为０．１０９７％、０．１２５６％、０．１５６８％。     

木里蝶类多样性的研究

研究了处于原始状态环境的蝶类多样性,种－多度曲线拟合于对数级数模型,物种少而生境不相互重叠,优势种得到大的发展,多样性指数与海拔高度不相关（ｒ＝－０．２９６８）,不同高度都有适宜或不适宜蝶类的生境。等级多样性２．９９８～８．３１３。多样性指数与均匀度不相关（ｒ＝０．２５６６,ｐ〉０．０５）,与丰富度相关（ｒ＝０．８５０６,ｐ〈０．０１）,群落间相似性系数０．０００～０．０６１,二维极点排序图及相关     

天尖杉科的核形态学研究

４种三尖杉科植物的形态结构的研究结果如下：它们的间期核均为复杂沟色中心型,分裂前期染色体属于中间型,体细胞分裂中期染色体的核型为：（１）三尖杉Ｃｅｐｈａｌｏｔａｘｕｓ ｆｏｒｔｕｎｅｉ Ｈｏｏｋ．ｆ．,Ｋ２ｎ＝２４＝２１ｍ＋１Ｍ＋２ｓｍ（２ＳＡＴ）。核型不对称性属于２Ａ型,着丝点端化值（Ｔ．Ｃ％）为５３．９９;（２）高山三尖杉Ｃ．ａｌｐｉｎａ（Ｌｉ）Ｌ．Ｋ．Ｆｕ,Ｋ２ｎ＝２４＝１９ｍ＋３Ｍ＋２ｓｍ     

矮生鸡冠花的离体快繁及试管苗开花

１植物名称矮生鸡冠花（Ｃｅｌｏｓｉａｃｒｉｓｔａｔａ）。２材料名称无菌种子苗顶芽、腋芽。３培养条件基本培养基为ＭＳ。培养基组合为：（１）ＭＳ＋ＢＡ１－２ｍｇ·Ｌ~（－１）（单位下同）＋ＮＡＡ０．１～０．２;（２）ＭＳ＋ＢＡ２＋ＩＡＡ０．２;（３）ＭＳ＋ＫＴ２;（４）ＭＳ＋ＢＡ０．１～０．５。以上培养基均含３％蔗糖,０．６％琼脂,ｐＨ为５．８,培养温室为（２６±２）℃,光照１２ｈ．ｄ~（－１）（２０００ｌｘ）。４生长与分化情况４．１无菌材料的获得成熟的种子置７０％酒精中摇动５０ｓ后,转入饱和漂白粉溶…     

青年男性高原移居者最大摄氧量计算公式的推导

最大摄氧量（Ｖｏ２ｍａｘ）是评价人体体力的重要指标,其测定方法分直接法和间接法两种。目前所推导的间接计算公式都是在平原、或是在进入高原初期推导的,不适用于高原习服人群。本研究采用逐步回归的方法,推导出移居高原７－２７个月、不同高度的青年男性Ｖｏ２ｍａｘ间接计算公式。在海拔３６８０ｍ地区,Ｖｏ２ｍａｘ（Ｌ／ｍｉｎ）＝１．１５３１＋０．００７３２７身高（ｃｍ）＋０．０１６１３体重（ｋｇ）－０．００５８８３晨脉（ｂ／ｍｉｎ）－０．００４５３４运动心率（６０Ｗ,６／ｍｉｎ）,Ｒ＝０．７４５,Ｐ＜０．０１,ＳＳ＝３．７７９９;或Ｖｏ２ｍａｘ（Ｌ／ｍｉｎ）＝１．２１８６＋０．０１９８４体重（ｋｇ）＋０．０７２５９肺活量（Ｌ）－０．００６６５９晨脉（ｂ／ｍｉｎ）,Ｒ＝０．７１３,ｐ＜０．０１,ｓｓ＝３．９６３６。在４３５０ｍ地区,Ｖｏ２．ｍａｘ（Ｌ／ｍｉｎ）＝０．４９１７＋０．０１６８７体重（ｋｇ）＋０．１１０９肺活量（Ｌ）＋０．００１９８３屏气时间（Ｓ）,Ｒ＝０．７８１,Ｐ＜０．０１,ＳＳ＝２．１３５６。计算值与实测值比较,变异系数在１３％以内,结果准确可靠,适用于青年男性高原习服移居者。     

几种氨基酸—铜（Ⅱ）配合物的超氧化物歧化酶活性

用四氮唑蓝光化学还原法对所合成的ＫＣｕ（ＩＤＡ）（Ｓｅｒ）．２Ｈ２Ｏ、ＫＣｕ（ＩＤＡ）（Ａｌａ）．Ｈ２Ｏ、Ｃｕ（ＩＤＡ）（ｅｎ）、ＫＣｕ（ＩＤＡ）（Ｇｌｙ）．Ｈ２Ｏ和Ｃｕ（ＩＤＡ）．２Ｈ２Ｏ（ＩＤＡ＝Ｎ（羧基甲基）－甘氨酸,Ｓｅｒ＝丝氨酸,Ａｌａ＝丙氨酸,ｅｎ＝乙二胺,Ｇｌｙ＝甘氨酸）等５种氨基酸一铜（Ⅱ）配合物进行了活性测定,发现它们均具有天然超氧化物歧化酶活性,其活性依次为０．３４,０．４５．     

Entwicklungsgeschichte und Ultrastruktur von Pollenkitt und Exine bei nahe verwandten entomophilen und anemophilen Angiospermensippen derAlismataceae,Liliaceae, Juncaceae,Cyperaceae, Poaceae undAraceae

Some closely related members of the monocotyledonous familiesAlismataceae, Liliaceae, Juncaceae, Cyperaceae, Poaceae andAraceae with variable modes of pollination (insect- and wind-pollination) were studied in relation to the ultrastructure of pollenkitt and exine (amount, consistency and distribution of pollenkitt on the surface of pollen grains). The character syndromes of pollen cementing in entomophilous, anemophilous and intermediate (ambophilous or amphiphilous) monocotyledons are the same in principal as in dicotyledons. Comparing present with former results one can summarize: 1) The pollenkitt is always produced in the same manner by the anther tapetum in all angiosperm sub-classes. 2) The variable stickiness of entomophilous and anemophilous pollen always depends on the particular distribution and consistency of the pollenkitt, but not its amount on the pollen surface. 3) The mostly dry and powdery pollen of anemophilous plants always contains a variable amount of inactive pollenkitt in its exine cavities. 4) A step-by step change of the pollen cementing syndrome can be observed from entomophily towards anemophily. 5) From the omnipresence of pollenkitt in all wind-pollinated angiosperms studied one can conclude that the ancestors of anemophilous angiosperms probably have been zoophilous (i.e. entomophilous) throughout.

     

Identification and Characterization of the First Equine Parainfluenza Virus 5

正Dear Editor,Parainfluenza virus 5 (PIV5), known as canine parainfluenza virus in the veterinary field, is a negative-sense,nonsegmented, single-stranded RNA virus belonging to the Paramyxoviridae family (Chen 2018). The virus was first reported in primary monkey kidney cells in 1954 (Hsiung1972), then it has been frequently discovered in various     

Pathogenic Characterization and Full Length Genome Sequence of a Reassortant Infectious Bursal Disease Virus Newly Isolated in Pakistan

<正>Dear Editor,Infectious bursal disease (IBD) is one of the most important diseases of the poultry. The IBD virus (IBDV), a nonenveloped virus belonging to the Birnaviridae family with a genome consisting of two segments of double-stranded RNA (segments A and B), targets B lymphocytes of bursa of Fabricious leading to immunosuppression. In Pakistan,poultry farming is the second biggest industry and IBD is the second biggest disease threating the poultry sector.However, there is limited genome information of IBDV     

Similar aerosol emission rates and viral loads in upper respiratory tracts for COVID-19 patients with Delta and Omicron variant infection

Highlights
1 Aerosol emission rates of Delta or Omicron patients were similar.
2 Viral loads in upper respiratory tract of Alpha, Delta and Omicron patients were similar.
3 Viral loads in upper respiratory tract of vaccinated or unvaccinated Delta patients had no difference.     

Recombinant human interferon-α1b inhibits SARS-CoV-2 better than interferon-α2b in vitro

Highlights
1) A comprehensive evaluation method for anti-SARS-CoV-2 drugs was established based on RT-qPCR, TCID₅₀ method, and immunofluorescence.
2) A significant antiviral effect of rHuIFN-α1b was shown with EC₅₀=0.12 IU/mL in Vero cells and EC₅₀=0.52 IU/mL in Calu-3 cells, which was better than rHuIFN-α2b (EC₅₀=0.25 IU/mL in Vero cells and EC₅₀=2.48 IU/mL in Calu-3 cells).
3) rHuIFN-α1b has a good potential in the application of anti-COVID-19 therapy.