Are endophyte-mediated effects on herbivores conditional on soil nutrients?

Neotyphodium endophytes are assumed to have mutualistic relationship with their grass hosts, mainly resulting from mycotoxin production increasing plant resistance to herbivores by the fungus that subsists on the plant. To study importance of often ignored environmental effects on these associations, we performed a greenhouse experiment to examine the significance of endophyte infection and nutrient availability for bird-cherry aphid (Rhopalosiphum padi) performance on meadow fescue (Lolium pratense). Naturally endophyte-infected (E+), uninfected (E–), or manipulatively endophyte-free (ME–) half-sib families of meadow fescue were grown on two soil nutrient levels. Endophyte infection reduced aphid performance in general. However, to our knowledge, this is the first study to demonstrate experimentally that herbivore performance decreases on E+ host plants with increasing availability of nutrients in soils. Potential improvement in herbivore performance in high nutrient soils and decreased plant performance in low nutrient soils in ME– plants, compared to E– and E+ plants, suggests that loss of endophyte infection after long coevolutionary relationship may be critical to plant fitness.     

Life on Jupiter?

The possibilities of life on Jupiter are discussed from the point view of life as we know it. That is, we assume that any life on Jupiter would not involve new principles foreign to us. Proteins would be a constituent as would fats and the other building blocks of living organisms on Earth. This leads us to a set of limiting parameters, such as pressure. Studies in the laboratory have shown that proteins and other essential molecules are denatured by pressures of 4000 atm and higher. Thus, we must not expect life in the great depths of the Jovian atmosphere. It could exist only at depths of several hundred kilometers in the atmosphere. Since no solid surface could possibly exist at such altitudes, any organisms present must be small enough to be buoyed up by the turbulent atmospheric currents or must fly or both. Such possibilities, however, seem to be real. The necessary nutrients to preserve life and foster growth could be furnished by the Miller-Urey type reactions of lonizing radiation on the reducing atmosphere undoubtedly present. There can, of course, be no possibility of oxygen on Jupiter, and so the life forms, if they exist, must be anaerobic. Such possibilities are real and have often been cited in connection with the origin of life on Earth.     

Heteropolypeptides on Titan?

Since hydrogen cyanide is a component of Titan's hazy atmosphere, HCN polymers might also be present by way of a low energy pathway leading initially to the synthesis of polyaminomalonitrile. Subsequent reactions of HCN with the activated nitrile groups of this HCN homopolymer would then yield heteropolyamidines, readily converted to heteropolypeptides following contact with frozen water on the surface of Titan.Similar HCN polymers in the reducing atmospheres of Jupiter and Saturn could be major contributors to the yellow-brown-orange appearance of these giant planets.Any detection of such HCN chemistry by the Voyager missions or the pending Galileo probe would constitute evidence for the hypothesis that heteropolypeptides on the primitive Earth were synthesized directly from hydrogen cyanide and water without the intervening formation of -amino acids.Paper presented at the 6th College Park Colloquium, October 1981.     

Observations on the effect of hurricane “Carla” on insect activity

Observations in the laboratory under conditions of controlled temperature and relative humidity on the effects of low barometric pressures on insect activity resulting fron hurricane Carla, one of the largest and most destructive hurricanes to affect the middle coast of Texas, revealed that stable flies (STOMOXYS CALCITRANS (L.)), house flies (MUSCA DOMESTICA L.), and Mexican fruit flies (ANASTREPHA LUDENS (Loew)), cockroaches (BLATELLA GERMANICA (L.)), PERIPLANETA AMERICANA (L.), and BLABERUS GIGANTEUS (L.) and mosquitoes (AEDES AEGYPTI (L.), exhibited nervous activity concomitant with the reduction in barometric pressure. Field studies throughout the critical storm period also indicated unusual arthropod activity. Following the hurricane, certain insect species emerged in abnormally large numbers, particularly fall armyworms (LAPHYGMA FRUGIPERDA (J.E. Smith)), asps (MEGALOPYGE OPERCULARIS (J. E. Smith)), and southern green stink bugs (NEZARA VIRIDULA (L.)).

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Zusammenfassung WÄhrend des Hurrikans Carla, einer der grössten und verheerendsten Orkane, die je auf die mittlere Küste von Texas eingewirkt haben, wurden im Laboratorium mit kontrollierter Temperatur und Feuchte, die Wirkungen von niedrigem Barometerdruck auf die InsektenaktivitÄt untersucht. Stallfliegen (STOMOXYS CALCITRANS (L.)), Hausfliegen (MUSCA DOMESTICA L.), Mexikanische Fruchtfliegen (ANASTREPHA LUDENS (Loew)), Schaben (BLATELLA GERMANICA L.), PERIPLANETA AMERICANA (L.) und BLABERUS GIGANTEUS (L.) und Mücken (AEDES AEGYPTI (L.)) zeigten wÄhrend der Verminderung des Barometerdruckes eine verstÄrkte AktivitÄt. Bei Studien im Freien wÄhrend der kritischen Orkanperiode wurde ebenfalls eine ungewöhnlich grosse AktivitÄt der Arthopoden beobachtet. Nach dem Orkan tauchten bestimmte Insektenarten in abnorm grosser Zahl auf. besonders (LAPHYGMA FRUGIPERDA (J.E.Schmith)), (MEGALOPYGE OPERCULARIS(J.E.Schmith)) und. (NEZARA VIRIDULA (L.)).

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Résumé L'effet, sur l'activité des insectes, des basses pressions barométriques résultant de l'ouragan Carla, un des plus vastes et des plus destructeurs qui aient affecté la cÔte moyenne du Texas, a été. observé au laboratoire dans des conditions de température et d'humidité relative constantes. Les mouches des étables (STOMOXYS CALCITRANS (L.)), les mouches domestiques (MUSCA DOMESTICA L.), les mouches à fruits mexicaines (ANASTREPHA LUDENS (Loew)), les cafards (BLATELLA GERMANICA (L.)), PERIPLANETA AMERICANA (L.) et BLABERUS GIGANTEUS (L.) et les moustiques (AEDES AEGYPTI (L.)) ont manifesté une activité accrue correspondant à la réduction de la pression barométrique. Des études faites en plein air pendant la période d'ouragan ont également permis d'observer chez les arthropodes une activité inaccoutumée. A la suite de l'ouragan sont apparus en quantités anormales certains insectes tels que le(LAPHYGMA FRUGIPERDA (J.E. Smith)), le (MEGALOPYGE OPERCULARIS (J.E.Smith)) et le (NEZARA VIRIDULA (L.)).

     

When to rely on maternal effects and when on phenotypic plasticity?

Existing insight suggests that maternal effects have a substantial impact on evolution, yet these predictions assume that maternal effects themselves are evolutionarily constant. Hence, it is poorly understood how natural selection shapes maternal effects in different ecological circumstances. To overcome this, the current study derives an evolutionary model of maternal effects in a quantitative genetics context. In constant environments, we show that maternal effects evolve to slight negative values that result in a reduction of the phenotypic variance (canalization). By contrast, in populations experiencing abrupt change, maternal effects transiently evolve to positive values for many generations, facilitating the transmission of beneficial maternal phenotypes to offspring. In periodically fluctuating environments, maternal effects evolve according to the autocorrelation between maternal and offspring environments, favoring positive maternal effects when change is slow, and negative maternal effects when change is rapid. Generally, the strongest maternal effects occur for traits that experience very strong selection and for which plasticity is severely constrained. By contrast, for traits experiencing weak selection, phenotypic plasticity enhances the evolutionary scope of maternal effects, although maternal effects attain much smaller values throughout. As weak selection is common, finding substantial maternal influences on offspring phenotypes may be more challenging than anticipated.     

Folding up and Moving on—Nascent Protein Folding on the Ribosome

All cellular proteins are synthesized by the ribosome, an intricate molecular machine that translates the information of protein coding genes into the amino acid alphabet. The linear polypeptides synthesized by the ribosome must generally fold into specific three-dimensional structures to become biologically active. Folding has long been recognized to begin before synthesis is complete. Recently, biochemical and biophysical studies have shed light onto how the ribosome shapes the folding pathways of nascent proteins. Here, we discuss recent progress that is beginning to define the role of the ribosome in the folding of newly synthesized polypeptides.     

Ammonium adsorption on Brønsted acidic centers on low-index vanadium pentoxide surfaces

Vanadium-based catalysts are used in many technological processes, among which the removal of nitrogen oxides (NO_x) from waste gases is one of the most important. The chemical reaction responsible for this selective catalytic reaction (SCR) is based on the reduction of NO_x molecules to N₂, and a possible reductant in this case is pre-adsorbed NH₃. In this paper, NH₃ adsorption on Brønsted OH acid centers on low-index surfaces of V₂O₅ (010, 100, 001) is studied using a theoretical DFT method with a gradient-corrected functional (RPBE) in the embedded cluster approximation model. The results of the calculations show that ammonia molecules are spontaneously stabilized on all low-index surfaces of the investigated catalyst, with adsorption energies ranging from ?0.34 to ?2 eV. Two different mechanisms of ammonia adsorption occur: the predominant mechanism involves the transfer of a proton from a surface OH group and the stabilization of ammonia as an NH₄ ⁺ cation bonded to surface O atom(s), while an alternative mechanism involves the hydrogen bonding of NH₃ to a surface OH moiety. The latter binding mode is present only in cases of stabilization over a doubly coordinated O(2) center at a (100) surface. The results of the calculations indicate that a nondirectional local electrostatic interaction with ammonia approaching a surface predetermines the mode of stabilization, whereas hydrogen-bonding interactions are the main force stabilizing the adsorbed ammonia. Utilizing the geometric features of the hydrogen bonds, the overall strength of these interactions was quantified and qualitatively correlated (R?=?0.93) with the magnitude of the stabilization effect (i.e., the adsorption energy).

Working on Sundays–effects on safety, health, and work-life balance

Several attributes of the work schedule can increase the risk of occupational injuries and accidents, health impairments, and reduced social participation. Although previous studies mainly focused on the effects of shiftwork and long working hours on employee health and safety, there is little evidence of a potential negative impact of working Sundays on the incidence of occupational accidents, health impairments, and work-life balance. A representative sample of employed workers in 31 member and associated states of the European Union (n?=?23,934) served as the database for a cross-sectional analysis. The sample was collected via face-to-face interviews in the year 2005. The association of the risks of occupational accidents, health impairments, and decreases in work-life balance with working Sundays was calculated using logistic regression models, controlling for potential confounders, such as shiftwork, workload, and demographic characteristics. The results indicated that working one or more Sundays/month was associated with increase both in the risk of reporting one or more health impairments (odds ratio [OR]: 1.17, 95% confidence interval [CI]: 1.06-1.29) and poorer work-life balance (OR: 1.15, 95% CI: 1.02-1.28). These effects remained after controlling for potentially confounding factors, such as other work schedule attributes, intensity of physical and mental workload, and individual characteristics. Furthermore, working Sundays was also related to increased risk of occupational accidents within the last year (OR: 1.34, 95% CI: 1.03-1.73). Controlling again for individual, workload, and working-time characteristics, a significant association with accident risk, however, remained only in work sectors with low a priori risk of occupational accidents (OR: 1.40, 95% CI: 1.02-1.91), although the increased risk could be observed for both medium and high a priori risk sectors working Sundays (without controlling for additional confounders). The results thus indicate that the detrimental effects of working Sundays on safety, health, and social well-being should be taken into account when designing work schedules. The potential hazards to employees' safety, health, and work-life balance, in particular, should be considered in discussions concerning extending work on Sundays in certain sectors, e.g., retail.     

Studies on the Effect of Water-Soluble Polymers on Drug–Cyclodextrin Complex Solubility

The effect of complexation of irbesartan (IRB), a practically water-insoluble drug, with cyclodextrins in presence of different concentrations of water-soluble polymers (PEG 4000 and PVP K-90) on the dissolution rate of the drug has been investigated. Phase solubility studies were carried out to evaluate the solubilizing power of βCD in association with water-soluble polymers towards IRB and to determine the apparent stability constant (K _S) of the complexes. Improvement in K _S value for ternary complexes (IRB–βCD–polymers) clearly proved the benefit on the addition of water-soluble polymer to increase complexation efficiency. The dissolution rate of the drug from ternary systems containing PEG 4000 and PVP K-90 was higher as compared to the binary system. An optimum increase in the dissolution rate of the drug was observed at a polymer concentration of 5% w/w for PVP K-90 and 10% w/w for PEG 4000. DSC, FTIR, SEM, and XRD studies were carried out to characterize the complexes.     

Nanjing Declaration on Nitrogen Management

The participants of the Third International Nitrogen Conference, held in Nanjing, People's Republic of China, 12-16 October 2004. AFFIRM the principles of the Millennium Development Goals and the World Summit on Sustainable Development to speedily increase access to basic human needs such as energy, water, food security and the protection of human health and biodiversity. AFFIRM the scientific findings of the International Nitrogen Conferences and the International Nitrogen Initiative (INI).     

Notes on the Potentilleæ

Background: Various rare and endangered temperate ferns are being threatened by their recent population decline, but there is limited understanding of the causes behind it.

Aims: This study attempted to identify the possible drivers of regional population decline and extinction in the globally distributed woodland fern Polystichum braunii.

Methods: A comparison was undertaken of the climatic, edaphic and phytosociological characteristics of sites with increasing, decreasing or recently extinct populations in Germany.

Results: A significantly higher frequency of episodes of low relative air humidity (<60%) was found at sites with decreasing or extinct populations compared to habitats with population increases. Sites with decreasing or extinct populations were also characterised as having less summer precipitation (<500 mm year^?1) and a shorter duration of snow cover (<110 days year^?1) than sites with increasing populations. The latter had significantly higher moss cover (56% of the forest floor), but less cover by a tree litter layer (23%) compared to decreasing (36% and 38%) or recently extinct populations (22% and 52%). All increasing populations were located in intact Tilia – Acer ravine forests, while those suffering population decline were mostly located in Fagus-dominated forests.

Conclusions: It was concluded that the probable causes of the recent decline in German P. braunii populations are reduced air humidity levels, decreasing snow duration or a shift from moss-covered to tree litter-covered forest floors due to climate warming or altered forest management.     

What's going on post-budding?

In general, the retrovirus particles become infectious on post-budding with cleavages of structural protein Gag by viral protease. Protease defective mutants bud particles normally, but the particles are non-infectious and called donuts-like particle because of their morphology. The viral genomes inside the donuts-like particles form very fragile dimer, which are far different from those in wild-type particles. The ordered particle maturation process is essential for infectivity of virus, but its mechanism largely remains unclear. We have constructed HIV-1 Gag cleavage site mutants to enable the steady state observation of virion maturation steps, and precisely study Gag processing, RNA dimerization, virion morphology and infectivity. As results, we found that these process progressed synchronously, but each transition point did not coincide completely. The mutual relationship between viral protein and RNA maturation is discussed for a further understanding of the retroviral life cycle.