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菜豆重金属胁迫响应基因:cDNA克隆及其表达分析 总被引:13,自引:0,他引:13
通过判别筛选HgCl2胁迫下的菜豆叶片cDNA库,分离出7组不同的cDNA克隆(Phaseolus vulgaris stress-relatedprotein,PvSR1-7)。cDNA序列和同源性分析结果表明:PvSR1编码富含脯氨酸细胞壁蛋白, 相似文献
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部分裸子植物叶片总蛋白分析 总被引:1,自引:0,他引:1
采用SDS- PAGE 技术, 分析了红豆杉科(Taxaceae) 植物南方红豆杉( Taxus chinensisvar- mairei (Lemee et Levl-) Cheng et L-K-Fu) 、穗花杉( Amentotaxus argotaenia (Hance) Pil ger) 、云南穗花杉( A- yunnanensis Li) 、白豆杉( Pseudotaxuschienii(Cheng) Cheng) 以及三尖杉科(Cephalotaxaceae) 、植物三尖杉( Cephalotaxus fortunei Hook-f-) 、粗榧( C-sinensis (Rehd-etWils-) Li) 、海南粗榧( C-hainanensis Li) 、篦子三尖杉( C-oliveri Mast-) 和罗汉松科(Podocarpaceae) 、植 物罗汉松 ( Podocarpus macrophyllus ( Thunb- ) D-Don) 、鸡毛 松( P-imbricatus Bl-) 、竹柏( P- nagi(Thunb-) Zoll) 、陆均松( Dacrydium pierrei Hickel) 共12 种植物的叶片蛋白, 在蛋白质水平上采用 相似文献
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盐浓度对3种单子叶盐生植物渗透调节剂及其在渗透调节中贡献的影响 总被引:21,自引:0,他引:21
单子叶盐生植物星星草(Puccinelliatenuiflora(Griseb).Scribn.et.Merr.)碱草(Aneurolepidiumchinese(Trins.)Kitag)和獐茅(Aeluropussinensis(Debeaux)Tzvel)的种子萌发后长至3cm高,用0,50,100,200和400mmol/LNaCl处理2周后,测定3种植物幼苗的生长,无机和有机渗透调节剂, 相似文献
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包有蓖麻毒蛋白的脂质体的制备,性质及毒性研究 总被引:10,自引:0,他引:10
以半乳糖神经酰胺(galactosylceramide,GC)为材料,采用冷冻干燥脱水再水化法(DRV法)制成包裹有蓖麻毒蛋白的半乳糖神经酰胺脂质体(ricinGCLs)并对其性质及体外细胞毒性和动物毒性做了研究,实验结果表明,ricin-GCLs的最大包封率可达74.1%,粒度分布主要在0.5~0.8μm之间,符合静脉注射液的标准,脂质体冻干粉在4℃储存近半年,粒度分布几乎没有变化,体外细胞毒性 相似文献
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为了进一步研究Elymius sibiricus L.,E.nutans Griseb.和E.burchan-buddae(Neuski)Tzelev[=Rogegneria nutans (kang) Keng] 的外部形态差及其系统学关系,本文对这三种植物的6个穗部形态性态状进行了观测和比较,并对这三个Elymus种进行了种间杂交及杂种F1的减数分裂染色体配对行为的分析研究。结果表明:这三个E 相似文献
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澜沧蔗茅 新种 图1ErianthuslancangensisY-Y-Qiansp-nov-SpeciesaffinisE-rufipilo(Steud-)Griseb-sedligulis2~3mmlongis,apiceobtusis,paniculis13~22-nodis,ramis1~9cmlongis,spiculis3-6~4mmlongis,glumisprimisnervis4-raro2~3,lemmatibussecundis1-1~1-3mm1ongis,dentibu… 相似文献
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毛竹茎细胞壁半纤维素多糖的免疫细胞化学定位研究 总被引:4,自引:1,他引:3
本文以毛竹(Phyllostachys pubescens)为材料,采用免疫细胞化学标记方法对两种细胞壁半纤维素多糖成分,即木聚糖(Xylan)和(1-3)(1-4)-β-葡聚糖「(1-3)(1-4)-β-glucan」在毛竹茎中的分布进行了观察。结果表明,应用免疫细胞化学方法可以准确、有效地观察这两种半纤维素多糖成分在细胞壁中的分析;木聚糖分布在已木质化的组织细胞的细胞壁中,与细胞壁木质化有密切 相似文献
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通过差别筛选HgCl2胁迫下的菜豆叶片cDNA库,分离出7组不同的cDNA克隆(Phaseolusvulgarisstress-relatedprotein,PvSR1~7)。cDNA序列和同源性分析结果表明:PvSR1编码富含脯氨酸细胞壁蛋白(PRP),PvSR2和PvSR7编码新的HgCl2胁迫相关蛋白,PvSR3编码脱水蛋白(dehydrin),PvSR4编码病原相关(PR)蛋白,PvSB5编码polyubiqui-tin,PvSR6编码DuaJ-like蛋白。HgCl2胁迫可强烈地请导PvSR2和PR蛋白基因的表达,并能提高PRP,、dehydrinlike和polyubiq-uitin基因的转录水平。这些蛋白质共同作用可能对维持细胞的正常代谢和抵抗重金属胁迫方面有重要作用。 相似文献
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菜豆多聚泛肽基因在重金属胁迫下的表达 总被引:1,自引:0,他引:1
差别筛选HgCl2胁迫的菜豆(PhaseolusvulgarisL.)幼苗叶片cDNA库,分离出两个重金属胁迫相应基因PvSR5和PvSR51(Phaseolusvulgarisstress_relatedgene)片段。cDNA和氨基酸序列分析表明PvSR5和PvSR51分别编码一种多聚泛肽。Northernblot分析表明多聚泛肽是组成性表达蛋白,主要在根中表达,叶片和茎中表达较少;Hg、Cd、Cu和Zn等重金属、高温和水杨酸能强烈地刺激其在叶片中的表达,而受伤几乎没有影响。推测多聚泛肽在抵抗重金属胁迫和提高植物的抗逆性方面有重要作用。 相似文献
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Cloning and expression analysis of SKn-type dehydrin gene from bean in response to heavy metals 总被引:6,自引:0,他引:6
A heavy metal responsive gene PvSR3 (GenBank accession number U54703) encoding an acid dehydrin was isolated from a mercuric chloride-treated bean (Phaseolus vulgaris L.) leaf cDNA library by differential screening using cDNAs derived from treated and untreated plants. The PvSR3 cDNA is 981-bp long and has a 606-bp open-reading frame with a 202-residue-deduced amino acid sequence. The PvSR3 sequence contains two conserved repeats of the characteristic lysine-rich K segment (EKKGIMDKIKEKLPG) preceded by an 8-serine residue stretch, whereas the Y segment (DEYGNP) conserved motif is absent. The deduced protein has a calculated molecular weight of 23 kDa and an isoelectric point of 5.2. Sequence similarity and comparative analysis showed that PvSR3 shares 70 and 73% similarity with the dehydrin of poplar and pepper, respectively. Southern hybridizations indicated that PvSR3 was a low copy-number gene. Northern blot analysis revealed that PvSR3 mRNA was weakly detected in seedling leaves. However, the gene expression was strongly stimulated by heavy metals, such as mercury, cadmium, arsenic, and copper, whereas virus infection and salt had little effect on it. In contrast, PvSR3 was not responsive to drought or abscisic acid (ABA), and was downregulated by UV radiation. Furthermore, PvSR3 was upregulated by the exogenous signaling molecules, including salicylic acid (SA) and hydrogen peroxide (H2O2). It is suggested that PvSR3 is extremely related to heavy metal stress, and might play an important role in metal detoxification and resistance to the damage caused by heavy metals. 相似文献
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DnaJ—like基因在不同环境胁迫下的表达研究 总被引:5,自引:0,他引:5
PvSR6(Phaseolus vulgaris stress-related)基因编码一种菜豆DnaJ-like蛋白。North-ern blot结果表明:PvSR6基因在未处理的菜豆叶片中表达较少,重金属(Hg^2+和Cd^2+)、机械损伤、UV、高温和水杨酸等环境胁迫能强烈地促进其基因的转录,推测DnaJ-like蛋白在保护细胞膜和酶蛋白的结构和功能及提高植物的抗逆性方面有重要作用。 相似文献
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菜豆病程相关蛋白基因在重金属胁迫下的表达分析 总被引:9,自引:0,他引:9
为探讨植物抗重金属的分子机理 ,差别筛选了 Hg Cl2 胁迫的菜豆 ( Phaseolus vulgaris L.)叶片 c DNA库 ,分离出一个重金属胁迫响应基因 Pv SR4克隆 .c DNA和氨基酸序列分析表明 Pv SR4编码一种细胞内病程相关蛋白 ,该蛋白具有 RNase活性 .Northern blot分析表明 Pv SR4基因在正常生长条件下的叶片中不表达 ,重金属 ( Hg、Cd、As、Zn和 Cu等 )和水杨酸能强烈地诱导其基因的表达 ,受伤也能促进该基因的转录 ,而热胁迫几乎没有调节作用 .推测 Pv SR4蛋白在诱导植物的抗逆性和抵抗重金属胁迫方面有重要作用 相似文献
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Yuxiu Zhang Jinmei Li Fei Yu Lin Cong Liyan Wang Gérard Burkard Tuanyao Chai 《Molecular biotechnology》2006,32(3):205-217
A heavy metal responsive gene PvSR3 (GenBank accession number U54703) encoding an acid dehydrin was isolated from a mercuric chloride-treated bean (Phaseolus vulgaris L.) leaf cDNA library by differential screening using cDNAs derived from treated and untreated plants. The PvSR3 cDNA is 981-bp long and has a 606-bp open-reading frame with a 202-residue-deduced amino acid sequence. The PvSR3 sequence
contains two conserved repeats of the characteristic lysine-rich K segment (EKKGIMDKIKEKLPG) preceded by an 8-serine residue
stretch, whereas the Y segment (DEYGNP) conserved motif is absent. The deduced protein has a calculated molecular weight of
23 kDa and an isoelectric point of 5.2. Sequence similarity and comparative analysis showed that PvSR3 shares 70 and 73% similarity
with the dehydrin of poplar and pepper, respectively. Southern hybridizations indicated that PvSR3 was a low copy-number gene. Northern blot analysis revealed that PvSR3 mRNA was weakly detected in seedling leaves. However, the gene expression was strongly stimulated by heavy metals, such as
mercury, cadmium, arsenic, and coppper, whereas virus infection and salt had little effect on it. In contrast, PvSR3 was not responsive to drought or abscisic acid (ABA), and was downregulated by UV radiation. Furthermore, PvSR3 was upregulated by the exogenous signaling molecules, including salicylic acid (SA) and hydrogen peroxide (H2O2). It is suggested that PvSR3 is extremely related to heavy metal stress, and might play an important role in metal detoxification and resistance to the
damage caused by heavy metals. 相似文献
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Cadmium resistance in transgenic tobacco plants enhanced by expressing bean heavy metal-responsive gene PvSR2 总被引:6,自引:0,他引:6
Heavy metal pollution such as Cd, Hg, Pb, As and Se is an increasing environment problem worldwide. These metals and metalloids have toxic effect on both plants and animals, which are strongly poisonous to metal-sensitive enzymes, resulting in growth inhibition and death of the organism[1]. Contamination of soils with heavy metals, either by natural causes or due to pollution, often has pronounced effects on the vegetation, resulting in the appearance of metallophytes, and heavy-metal tolera… 相似文献
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PvSR2 (Phaseolus vulgaris stress-related gene) has been cloned from French bean and shown to be expressed specifically upon heavy metal treatment. In order to investigate the role of PvSR2 in plant, PvSR2 gene under the control of cauliflower mosaic virus 35S promoter was introduced into tobacco mediated with Agrobacterium tumefaciens LBA4404. The regenerated plantlets were selected on medium with 100 mg/L kanamycin. PCR and Southern blot analysis showed PvSR2 gene was integrated in tobacco genome. Gus and Northern blot analysis indicated PvSR2 gene was expressed in transgenic seedling. The heavy metal resistance assay showed that the transgenic tobacco seedlings with the PvSR2 coding sequence exhibited higher tolerance to Cd compared with wild-type (WT) under Cd exposure. The Cd content accumulated in root between transgenic and WT seedlings had no obvious difference at lower Cd external concentration (0.05-0.075 mmol/L CdCl2), whereas transgenic plant showed a lower root Cd content than the control at higher external Cd concentration (0.1 mmol/L CdCl2). These results suggested that the expression of PvSR2 can enhance the Cd tolerance, and PvSR2 may be involved in Cd transportation and accumulation at the test concentration of 0.1 mmol/L Cd. 相似文献
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差别筛选HgCl2胁迫处理的菜豆(Phaseolus vulgaris L.)幼苗叶片cDNA库,分离出1个重金属胁迫响应基因PvSR52克隆,其cDNA长度为281bp。cDNA和氨基酸序列同源性分析表明PvSR52编码一种多聚泛肽。Southern blot结果表明菜豆泛肽可能由少数基因编码。Northern blot分析表明多聚泛肽叶片中表达较少;重金属Hg、Cd和As等、过量的Zn和Cu及高温、病毒侵染和水杨酸等环境胁迫均能强烈地刺激其在叶片中的表达。推测泛肽水解系统在提高植物的抗塑性方面有重要作用。 相似文献