The variation of soil microbial respiration with depth in relation to soil carbon composition

The vertical variation in soil microbial respiratory activity and its relationship to organic carbon pools is critical for modeling soil C stock and predicting impacts of climate change, but is not well understood. Mineral soil samples, taken from four Scottish soils at different depths (0–8, 8–16, 16–24, 24–32 cm), were analyzed and incubated in the laboratory under constant temperature and environmental conditions. The vegetation type/plant species showed significant effects on the absolute concentration of C components and microbial activity, but the relative distribution of C and respiration rate with soil depth are similar across sites. Soil C pools and microbial respiratory activity declined rapidly with soil depth, with about 30% of total organic carbon (TOC) and dissolved organic carbon (DOC), and about half microbial carbon (C_mic) and respired CO₂ observed in the top 8 cm. The ratio of CO₂:TOC generally decreased with soil depth, but CO₂:DOC was significantly higher in the top 8 cm of soil than in the subsoil (8–32 cm). No general pattern between qCO₂ (CO₂:C_mic) and soil depth was found. The vertical distributions of soil C pools and microbial respiratory activity were best fitted with a single exponential equation. Compared with TOC and DOC, C_mic appears to be an adequate predictor for the variation in microbial respiration rate with soil depth, with 95% of variation in normalized respiration rate accounted for by a linear relationship.     

Dissolved Organic Carbon in Headwater Streams and Riparian Soil Organic Carbon along an Altitudinal Gradient in the Wuyi Mountains,China

Stream water dissolved organic carbon (DOC) correlates positively with soil organic carbon (SOC) in many biomes. Does this relationship hold in a small geographic region when variations of temperature, precipitation and vegetation are driven by a significant altitudinal gradient? We examined the spatial connectivity between concentrations of DOC in headwater stream and contents of riparian SOC and water-soluble soil organic carbon (WSOC), riparian soil C:N ratio, and temperature in four vegetation types along an altitudinal gradient in the Wuyi Mountains, China. Our analyses showed that annual mean concentrations of headwater stream DOC were lower in alpine meadow (AM) than in subtropical evergreen broadleaf forest (EBF), coniferous forest (CF), and subalpine dwarf forest (SDF). Headwater stream DOC concentrations were negatively correlated with riparian SOC as well as WSOC contents, and were unrelated to riparian soil C:N ratio. Our findings suggest that DOC concentrations in headwater streams are affected by different factors at regional and local scales. The dilution effect of higher precipitation and adsorption of soil DOC to higher soil clay plus silt content at higher elevation may play an important role in causing lower DOC concentrations in AM stream of the Wuyi Mountains. Our results suggest that upscaling and downscaling of the drivers of DOC export from forested watersheds when exploring the response of carbon flux to climatic change or other drivers must done with caution.     

氮沉降对长白山森林土壤团聚体内碳、氮含量的影响

氮沉降是影响陆地生态系统碳、氮循环的最重要因素之一.为了解土壤团聚体碳、氮组分对氮沉降的响应,本研究在长白山选取次生杨桦林(YHL)与原始阔叶红松林(HSL)两种林型进行为期6年的氮添加试验,采集土壤样品并分析氮沉降对不同粒径土壤团聚体中可溶性有机碳、氮(DOC和DON)、微生物生物量碳、氮(MBC和MBN)、颗粒有机碳、氮(POC和PON)的影响.结果表明: 除POC和PON外,两林分土壤团聚体碳、氮组分含量均随团聚体粒径的减小而增加;氮添加处理显著降低了HSL土壤团聚体中POC和PON含量,降幅分别达20.7%和22.6%,显著增加了DOC含量,增幅达11.6%;氮添加处理对YHL土壤团聚体的碳、氮组分均无显著影响,其中,对DOC和MBC的影响接近于显著(0.05＜P＜0.1).皮尔森相关分析结果表明,土壤团聚体中总碳或总氮与碳、氮活性组分之间有良好的相关性,其中,HSL土壤的POC与DOC之间呈极显著负相关(r=-0.503),DOC又与MBC呈显著正相关关系(r=0.462).氮添加处理降低阔叶红松林土壤团聚体中POC和PON含量、增加DOC含量的主要原因是其促进了微生物对POM的分解,进而导致DOC的释放.阔叶红松林土壤碳、氮库对氮沉降的响应比次生杨桦林更加敏感.     

川西高山林线土壤活性碳、氮对短期增温的响应

随着温室效应的加剧,受低温限制的高山林线生态系统对全球气候变暖较为敏感,可能直接影响到植物的生长和土壤碳氮过程.本研究假设气候变暖会改变高山生态系统土壤活性碳氮含量,在四川省理县米亚罗高山生态系统定位站,采用开顶式模拟增温装置(OTC)模拟增温对土壤活性碳、氮的短期影响.分别于2017年4、7和10月,采集OTC以及对照样地(CK)内土壤有机层和矿质土壤层的原状土壤,测定土壤可溶性有机碳(DOC)、土壤微生物生物量碳(MBC)、土壤可溶性有机氮(DON)和土壤微生物生物量氮(MBN)含量.结果表明: 模拟增温使年均气温升高0.88 ℃,土壤有机层和矿质土壤层的年均温度分别提高0.48和0.23 ℃.模拟增温没有显著改变土壤有机质和含水量,但显著提高了矿质土壤层的pH值,同时显著降低了非生长季矿质土壤层的DOC、DON含量;季节变化对两个层次的DOC、DON和MBN含量有极显著影响,而MBC没有明显的季节动态;增温和季节交互作用对矿质土壤层的DOC和DON有显著影响.土壤有机层的MBC、MBN含量显著高于矿质土壤层.土壤活性碳、氮与土壤有机质和含水量呈极显著正相关,MBC、MBN与土壤pH呈极显著正相关,MBN与土壤温度呈显著负相关.     

亚热带米槠林不同更新方式对土壤可溶性有机质降解性的影响

可溶性有机质(DOM)的生物降解性影响着土壤有机质的存留和释放,对深入认识森林土壤养分循环意义重大。为探究森林更新对土壤DOM降解特征的影响,选取亚热带地区米槠天然林(NF)、米槠次生林(SF)和米槠人工促进天然更新林(AR)土壤DOM溶液为研究对象,进行室内降解(42 d)试验。结果表明: 1)3种林分土壤可溶性有机碳(DOC)的降解率和易降解DOC的比例均为SF>AR>NF;可溶性有机氮(DON)和微生物生物量碳(MBC)是显著影响易降解DOC比例的因子;2)难降解组分占3种林分土壤DOC的大部分(72.3%～94.6%),其周转时间长,有利于稳定土壤有机质(SOC)的形成;3)土壤DOM最初的腐殖化指数(HIX_em)会影响易降解DOC的周转时间。DOM光谱结构随降解过程呈现动态变化,说明DOM中易降解组分被消耗完后,微生物会转而降解芳香类和疏水性物质以获取碳源。综上,米槠天然林更新为次生林和人促林后增加了易降解DOC的比例,提高了土壤DOM生物可降解性,不利于SOC的积累。     

模拟氮沉降和降雨量改变对华西雨屏区常绿阔叶林土壤有机碳的影响

从2013年11月至2015年12月,通过原位试验,在华西雨屏区常绿阔叶林内设置了对照(CK)、氮沉降(N)、减雨(R)、增雨(A)、氮沉降+减雨(NR)、氮沉降+增雨(NA)6个处理水平,研究了模拟氮沉降和降雨量改变对常绿阔叶林土壤有机碳的影响。结果表明:华西雨屏区常绿阔叶林土壤各土层有机碳含量表现为夏季较高,春冬季较低,0—10 cm土层有机碳含量高于10—20 cm土层。从各处理土壤有机碳含量的平均值来看,0—10 cm土层土壤有机碳含量高低顺序表现为:RNRCKANNA;10—20 cm土层表现为:RNRACKNAN。模拟氮沉降和增雨处理促进了华西雨屏区常绿阔叶林土壤有机碳的累积,模拟减雨抑制了土壤有机碳的累积。常绿阔叶林0—10cm土层土壤C/N值显著高于10—20 cm,土壤C/N值随土层加深而呈现出增加的趋势,降雨使土壤C/N降低,增雨使土壤C/N增高。同一氮沉降条件下,增雨处理增加了土壤有机碳的含量,减雨处理减少了土壤有机碳的含量;同一降雨条件下,氮沉降增加土壤有机碳的含量。氮沉降和降雨对土壤可溶解性有机碳和微生物生物量碳含量产生显著影响(P0.05),对土壤活性碳含量影响不显著(P0.05);其交互作用对土壤有机碳、可溶解性有机碳、微生物生物量碳和活性碳含量影响不显著(P0.05)。     

Soil organic matter and litter chemistry response to experimental N deposition in northern temperate deciduous forest ecosystems

The effects of atmospheric nitrogen (N) deposition on organic matter decomposition vary with the biochemical characteristics of plant litter. At the ecosystem‐scale, net effects are difficult to predict because various soil organic matter (SOM) fractions may respond differentially. We investigated the relationship between SOM chemistry and microbial activity in three northern deciduous forest ecosystems that have been subjected to experimental N addition for 2 years. Extractable dissolved organic carbon (DOC), DOC aromaticity, C : N ratio, and functional group distribution, measured by Fourier transform infrared spectra (FTIR), were analyzed for litter and SOM. The largest biochemical changes were found in the sugar maple–basswood (SMBW) and black oak–white oak (BOWO) ecosystems. SMBW litter from the N addition treatment had less aromaticity, higher C : N ratios, and lower saturated carbon, lower carbonyl carbon, and higher carboxylates than controls; BOWO litter showed opposite trends, except for carbonyl and carboxylate contents. Litter from the sugar maple–red oak (SMRO) ecosystem had a lower C : N ratio, but no change in DOC aromaticity. For SOM, the C : N ratio increased with N addition in SMBW and SMRO ecosystems, but decreased in BOWO; N addition did not affect the aromaticity of DOC extracted from mineral soil. All ecosystems showed increases in extractable DOC from both litter and soil in response to N treatment. The biochemical changes are consistent with the divergent microbial responses observed in these systems. Extracellular oxidative enzyme activity has declined in the BOWO and SMRO ecosystems while activity in the SMBW ecosystem, particularly in the litter horizon, has increased. In all systems, enzyme activities associated with the hydrolysis and oxidation of polysaccharides have increased. At the ecosystem scale, the biochemical characteristics of the dominant litter appear to modulate the effects of N deposition on organic matter dynamics.     

Rapid turnover of DOC in temperate forests accounts for increased CO2 production at elevated temperatures

The evidence for the contribution of soil warming to changes in atmospheric CO₂ concentrations and carbon stocks of temperate forest ecosystems is equivocal. Here, we use data from a beech/oak forest on concentrations and stable isotope ratios of dissolved organic carbon (DOC), phosphate buffer-extractable organic carbon, soil organic carbon (SOC), respiration and microbial gross assimilation of N to show that respired soil carbon originated from DOC. However, the respiration was not dependent on the DOC concentration but exceeded the daily DOC pool three to four times, suggesting that DOC was turned over several times per day. A mass flow model helped to calculate that a maximum of 40% of the daily DOC production was derived from SOC and to demonstrate that degradation of SOC is limiting respiration of DOC. The carbon flow model on SOC, DOC, microbial C mobilization/immobilization and respiration is linked by temperature-dependent microbial and enzyme activity to global warming effects of CO₂ emitted to the atmosphere.     

放牧强度对华北农牧交错带典型草地土壤化学计量特征的短期影响

放牧是天然草地的主要利用方式之一,不同放牧强度可能通过影响家畜的选择性采食、凋落物输入和微生物的组成及结构等影响草地土壤化学计量特征。本研究通过在华北农牧交错带典型草地一个连续3年(2017—2019年)的生长季放牧试验,测定了土壤全碳(TC)、全氮(TN)、可溶性有机碳(DOC)和可溶性氮(DN)含量,以及土壤微生物生物量碳(MBC)和微生物生物量氮(MBN),分析这些参数间的化学计量特征,研究放牧强度对该地区草地土壤计量化学特征的影响。结果表明: 连续3年不同强度的放牧(1、2、4 sheep·0.2 hm^-2)对土壤TC含量没有显著影响,2019年中度放牧显著降低了10～20 cm土层中TN含量,轻度、中度和重度放牧显著提高10～20 cm层土壤的C/N。连续3年不同强度的放牧对土壤DOC、DN含量以及DOC/DN均没有显著影响,而DOC和DN含量在2019年呈现出随放牧强度增加而减少的趋势,表明持续高强度的放牧可能会引起土壤可溶性养分减少。随着放牧年限的增长,轻度放牧显著增加了土壤MBC,重度放牧显著降低了土壤MBC,而土壤MBN及MBC/MBN在不同放牧强度下变化不显著。     

不同土地利用类型下土壤活性有机碳库的变化

分析了中国科学院沈阳生态试验站不同土地利用类型长期定位试验土壤0～40cm活性有机碳含量,结果表明:0～20cm土层内荒地土壤有机碳、易氧化碳、微生物生物量碳、溶解性有机碳和轻组有机碳含量高于割草地和裸地,而割草地颗粒有机碳含量略高于荒地;在20～40cm土层,割草地土壤有机碳、易氧化碳和颗粒有机碳含量较高,而荒地微生物量碳、溶解性有机碳和轻组有机碳含量较高。不同土地利用类型土壤活性有机碳含量均随着土层加深而递减。土壤微生物量碳、溶解性有机碳和轻组有机碳的分配比例为荒地>割草地>裸地,易氧化碳和颗粒有机碳的分配比例为割草地>荒地>裸地。土壤活性有机碳的分配比例随土层加深而下降,但溶解性有机碳的分配比例变化趋势相反。     

Influence of Litter Diversity on Dissolved Organic Matter Release and Soil Carbon Formation in a Mixed Beech Forest

We investigated the effect of leaf litter on below ground carbon export and soil carbon formation in order to understand how litter diversity affects carbon cycling in forest ecosystems. ¹³C labeled and unlabeled leaf litter of beech (Fagus sylvatica) and ash (Fraxinus excelsior), characterized by low and high decomposability, were used in a litter exchange experiment in the Hainich National Park (Thuringia, Germany). Litter was added in pure and mixed treatments with either beech or ash labeled with ¹³C. We collected soil water in 5 cm mineral soil depth below each treatment biweekly and determined dissolved organic carbon (DOC), δ¹³C values and anion contents. In addition, we measured carbon concentrations and δ¹³C values in the organic and mineral soil (collected in 1 cm increments) up to 5 cm soil depth at the end of the experiment. Litter-derived C contributes less than 1% to dissolved organic matter (DOM) collected in 5 cm mineral soil depth. Better decomposable ash litter released significantly more (0.50±0.17%) litter carbon than beech litter (0.17±0.07%). All soil layers held in total around 30% of litter-derived carbon, indicating the large retention potential of litter-derived C in the top soil. Interestingly, in mixed (ash and beech litter) treatments we did not find a higher contribution of better decomposable ash-derived carbon in DOM, O horizon or mineral soil. This suggest that the known selective decomposition of better decomposable litter by soil fauna has no or only minor effects on the release and formation of litter-derived DOM and soil organic matter. Overall our experiment showed that 1) litter-derived carbon is of low importance for dissolved organic carbon release and 2) litter of higher decomposability is faster decomposed, but litter diversity does not influence the carbon flow.     

氮素添加和CO2浓度升高对白羊草根际和非根际土壤水溶性有机碳、氮的影响

采用盆栽控制试验对黄土丘陵区白羊草在不同CO₂浓度(400和800 μmol·mol^-1)和施氮水平(0、2.5、5.0 g N·m^-2·a^-1)条件下根际和非根际土壤水溶性有机碳(DOC)和水溶性有机氮(DON)的变化特征进行研究.结果表明: CO₂浓度升高对白羊草根际和非根际土壤DOC、水溶性总氮(DTN)、DON、水溶性铵态氮(NH₄⁺-N)、水溶性硝态氮(NO₃^--N)含量均无显著影响.施氮显著提高了根际和非根际土壤DTN、NO₃^--N含量和根际土壤DON含量,显著降低了根际土壤DOC/DON.在各处理条件下,根际土壤DTN、NO₃^--N和DON含量均显著低于非根际土壤,根际土壤DOC/DON显著高于非根际土壤.短期CO₂浓度升高对黄土丘陵区土壤水溶性有机碳、氮含量无显著影响,而氮沉降的增加在一定程度上改善了土壤中水溶性氮素缺乏的状况,但并不足以满足植被对水溶性氮素的需求.     

氮素添加和CO2浓度升高对白羊草根际和非根际土壤水溶性有机碳、氮的影响

采用盆栽控制试验对黄土丘陵区白羊草在不同CO₂浓度(400和800 μmol·mol^-1)和施氮水平(0、2.5、5.0 g N·m^-2·a^-1)条件下根际和非根际土壤水溶性有机碳(DOC)和水溶性有机氮(DON)的变化特征进行研究.结果表明: CO₂浓度升高对白羊草根际和非根际土壤DOC、水溶性总氮(DTN)、DON、水溶性铵态氮(NH₄⁺-N)、水溶性硝态氮(NO₃^--N)含量均无显著影响.施氮显著提高了根际和非根际土壤DTN、NO₃^--N含量和根际土壤DON含量,显著降低了根际土壤DOC/DON.在各处理条件下,根际土壤DTN、NO₃^--N和DON含量均显著低于非根际土壤,根际土壤DOC/DON显著高于非根际土壤.短期CO₂浓度升高对黄土丘陵区土壤水溶性有机碳、氮含量无显著影响,而氮沉降的增加在一定程度上改善了土壤中水溶性氮素缺乏的状况,但并不足以满足植被对水溶性氮素的需求.     

北方农牧交错带温性盐碱化草地土壤碳组分对模拟增温的响应机制

中国北方农牧交错带温性盐碱化草地土壤有机碳库对全球气候变暖的响应趋势存在较大不确定性。作为温性盐碱性草地的典型分布区，山西右玉农牧交错带是探索相关研究的理想生境。基于山西农业大学野外观测研究站开顶式气室模拟增温实验平台，通过采集生长旺季土壤样品，探索温性盐碱化草地不同土层有机碳、氮组分对模拟增温的响应与适应机制。结果表明：(1)不同增温处理对土壤有机碳(C)、总氮(N)、颗粒性有机碳(POM-C)和氮(POM-N)、矿物结合态有机碳(MAOM-C)和氮(MAOM-N)、可溶性有机碳(DOC)和氮(DON),以及微生物量碳(MBC)和氮(MBN)等组分无显著影响，但显著降低了MAOM-C/MBC的比值；(2)除土壤可溶性有机碳和微生物量碳外，土壤碳、氮各组分均随土层深度加深而呈现递减趋势，土壤碳、氮各组分之间的比值，除MAOM-N/N和MBC/C外，均随土层深度的增加而呈现显著上升趋势；(3)增温对POM-N/MBN和MAOM-N/MBN的影响与土层深度存在明显的交互效应；(4)不同土层氮组分比值对增温的响应与禾草丰度、杂类草丰度、凋落物量、土壤pH值及土壤含水量等因素有关。其中，凋落物...     

火烧迹地不同恢复方式土壤有机碳分布特征

以大兴安岭1987年重度火烧后恢复的兴安落叶松人工林、樟子松人工林、人促杨桦林和天然次生杨桦林为对象,研究不同恢复方式林分土壤有机碳、土壤可溶性有机碳和土壤微生物生物量碳的分布特征.结果表明: 4种恢复方式林分的土壤有机碳、土壤可溶性有机碳和土壤微生物生物量碳分别为9.63～79.72 g·kg^-1、33.21～186.30 mg·kg^-1和200.85～1755.63 mg·kg^-1,且随土层深度增加而降低.不同恢复方式间土壤有机碳、土壤可溶性有机碳和土壤微生物生物量碳差异显著,以人促杨桦林最高,兴安落叶松人工林和天然次生杨桦林次之,樟子松人工林最低.各恢复方式林分的土壤微生物熵为1.1%～2.3%,以人促杨桦林最高,樟子松人工林最低,不同林分土壤微生物熵的垂直分布特征不同.土壤微生物生物量碳与土壤有机碳、土壤可溶性有机碳含量均呈显著正相关.人促杨桦林土壤有机碳活性高于其他林分,火烧迹地采用人工促进天然恢复的方式较人工恢复和天然恢复的土壤碳循环能力更强.     

The influence of soil frost on the quality of dissolved organic carbon in a boreal forest soil: combining field and laboratory experiments

Riparian soils exert a major control on stream water dissolved organic carbon (DOC) in northern latitudes. As the winter climate in northern regions is predicted to be particularly affected by climate change, we tested the sensitivity of DOC formation to winter conditions in riparian soils using an 8?year field-scale soil frost manipulation experiment in northern Sweden. In conjunction with the field experiment, we also carried out a laboratory experiment based on three levels of four winter climatic factors: frost intensity, soil water content, frost duration and frequency of freeze–thaw cycles. We evaluated changes in lability of DOC in soil solution from lysimeter samples taken at different depths (10–80?cm) as well as from DOC extracted from soils in the laboratory, using carbon-specific ultraviolet absorbance at 254?nm (sUVA₂₅₄). In the field, significantly more labile DOC was observed during the spring and summer from upper horizons of frost-exposed soils, when compared to controls. In addition, the amount of labile DOC was positively correlated with frost duration at a soil depth of 10?cm. In the laboratory, frost intensity was the factor that had the greatest positive influence on DOC lability; it also reduced the C:N ratio which may indicate a microbial origin of the DOC. The laboratory experiment also demonstrated significant interactions between some of the applied climatic factors, such as frost intensity interacting with water content. In combination, field and laboratory experiments demonstrate that winter soil conditions have profound effects on DOC-concentration and quality during subsequent seasons.     

Importance of dissolved organic matter produced by duckweed (Lemna minor) in a southern English river

SUMMARY. 1. The quantity of dissolved organic carbon (DOC) released by axenic Lemna minor cultures in the laboratory has been determined by an absolute method and by ¹⁴C labelling.
2. Good agreement was obtained between the two methods and from 1.1% to 2.6% of the total carbon fixed was released.
3. The DOC produced by L. minor has been analysed by ultrafiltra- tion and compared with a similar analysis of DOC in natural river water.
4. The results of ullrafiltration analyses indicate that the proportion of low molecular weight (<1000 Daltons) material in DOM produced by axenic L. minor is significantly greater than is found in its natural habitat.     

不同稻作模式下稻田土壤活性有机碳变化动态

通过大田试验,研究了稻鸭复合种养(RD)、间歇灌溉(RW)、常规淹水灌溉(CK)3种不同稻作模式下稻田土壤可溶性有机碳(DOC)、易氧化态有机碳(ROC)和微生物生物量碳(MBC)3种活性有机碳组分的动态变化规律.结果表明:3种稻作模式下土壤DOC、MBC在水稻拔节-齐穗阶段含量最高;ROC在水稻整个生育期内变化平稳,没有表现出显著的阶段性差异.MBC受水稻生育期的影响最大;DOC受水稻生育期和稻作模式的影响均较大;而ROC主要受稻作模式的影响.与CK相比,RD能显著提高土壤DOC和ROC及其有效率;而RW极显著降低了DOC及其有效率,明显提高了ROC及其有效率.3处理间MBC及微生物熵均没有显著差异.     

不同灌水施肥策略对土壤微生物量碳氮和酶活性的影响

根区局部灌溉形成一个土壤水分分布不均匀的环境,影响了土壤微生物量和酶活性。为探明这种影响,在2种灌水水平(正常灌水和轻度缺水)和2种有机无机N肥配施(单施无机N肥和有机无机N肥配施)下,以常规灌溉(CI)为对照,研究分根区交替灌溉(AI)和固定部分根区灌溉(FI)对土壤微生物量C(MBC)、微生物量N(MBN)和酶活性的影响。与CI相比,AI提高拔节期土壤MBC和抽雄期土壤脲酶活性,但是降低大喇叭口期土壤MBC以及拔节期和抽雄期土壤MBN和拔节期土壤脲酶活性;FI增加抽雄期土壤转化酶活性,但是降低大喇叭口期土壤MBC和可溶性碳(DOC)以及3个时期土壤MBN。与正常灌水相比,AI下轻度亏水增加拔节期和抽雄期土壤DOC,但是降低大喇叭口期土壤MBC和拔节期土壤MBN。与单施无机N肥相比,AI下有机无机N肥配施增加拔节期土壤DOC、MBN和过氧化氢酶活性,而FI下则降低大喇叭口期土壤MBC和转化酶活性。因此,在轻度缺水和有机无机氮配施条件下,分根区交替灌溉可以提高玉米拔节期土壤微生物量碳和可溶性碳。     

不同培肥茶园土壤微生物量碳氮及相关参数的变化与敏感性分析

通过闽东地区茶园培肥长期定位试验,研究了不同培肥模式下土壤微生物量碳、氮,微生物量碳占溶解有机碳的比值和微生物熵的动态变化及其与其他土壤参数、茶叶理化性状的相关性。试验设6个处理:全量化肥(C),半量化肥+半量有机肥((CO)1/2),全量有机肥(O),全量化肥+豆科绿肥(CL),半量化肥+半量有机肥+豆科绿肥((CO)1/2L)和不施肥(CK)。研究结果显示:(CO)1/2L、CL和O等处理下土壤微生物量碳含量分别比CK增加了1.87、1.26、1.49倍,微生物量氮增加了2.18、1.32、1.70倍,而处理C的土壤微生物量碳、氮分别减少了0.46、0.59倍;微生物量碳占溶解有机碳的比值大小顺序为O>(CO)1/2L>CL>(CO)1/2>CK>C。可见,该区处理O和(CO)1/2L的培肥效果较佳。相关分析发现,微生物量碳及其占溶解有机碳的比值分别和土壤脲酶、有机质、全氮、全磷、水解氮、有效磷、速效钾、含水量、阳离子交换量等均呈显著正相关(P<0.05),表明它们与土壤肥力关系密切,可作为评价茶园土肥力变化的敏感指标。