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ABCG2/Bcrp1转运蛋白:侧群干细胞的表型标记与功能调控蛋白 总被引:4,自引:0,他引:4
在骨髓、骨骼肌及神经组织均发现侧群干细胞(SPSCs)。ABC转运蛋白ABCG2/Bcrp1基因在不同来源侧群(SP)干细胞均呈高表达,且该基因表达与SP细胞表型密切相关。SP细胞能向不同类型或不同胚层的组织细胞分化,很可能代表了一群更原始的干细胞,且与干细胞可塑性相关。而ABCG2/Bcrp1在造血及神经等组织来源的SP干细胞中的特异表达,使得该转运蛋白成为从不同组织中分选多潜能干细胞的一种新的表型标记。 相似文献
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成体干细胞研究中,SP细胞是近年来在多种不同种属的哺乳动物组织中被发现的细胞群体,是另一类主要在骨髓和肌肉中发现的多潜能干细胞,具有向不同于本身起源组织的多种细胞类型分化的能力。它们数目较少,且分布、分化、表面标志和功能等都不是十分清楚。综述了SP细胞的命名,分离获取方法,多种组织细胞中SP细胞群的表面标志、分化特征。此外,SP细胞表型的基因调控,SP细胞在干细胞移植尤其是在未来临床治疗方面的应用。最后,对SP细胞的研究进行了展望。 相似文献
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脐血CD-34单个核细胞来源间充质干细胞研究 总被引:2,自引:0,他引:2
目的 :探讨分离培养脐血CD-3 4 细胞来源间充质干细胞 (MSC)及研究其生物学特征。方法 :取足月妊娠健康产妇胎儿脐血 ,分离其中单个核细胞 (MNC) ,去除CD 3 4 细胞 ,体外用低糖型DMEM培养基培养。观察细胞形态、测定生长曲线、利用流式细胞仪对培养细胞进行表型测定、细胞周期分析、体外诱导分化实验以及检测造血因子的表达情况。结果 :脐血CD-3 4 细胞中可培养出间充质干细胞 ,可诱导向成骨和脂肪细胞分化并表达IL 6、SCF和SDF 1等造血生长因子。结论 :从足月妊娠健康产妇脐血CD-3 4 细胞可分离培养出间充质干细胞 ,具有与其它来源MSC类似的表型及分化潜能 ,在体外传代可保持其低分化状态并表达造血因子 ,可作为组织工程的种子细胞和具有促进造血作用 相似文献
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目的:探讨建立喉部黏膜间充质干细胞的分离培养方法,并对其生物学特性进行鉴定,为进一步研究其在喉部瘢痕中形成的作用及喉部组织工程提供参考.方法:以比格犬喉部会厌背侧(舌面)黏膜为研究对象,采用消化培养的方法分离具有间充质干细胞样细胞.选取第3代细胞对其进行生物学特性鉴定,首先利用MTT法检测其增殖活性及克隆形成情况,然后通过流式细胞术检测细胞表面分子标记物CD29及CD34的表达情况,最后应用第3代细胞对其进行成脂肪细胞和成骨细胞分化培养,观察其分化能力.结果:分离培养细胞形态较为一致,绝大多数呈梭形,排列不规则.MTT增殖活性实验及克隆形成试验结果显示,所分离的细胞具有良好的增殖活性和克隆形成率;流式细胞术结果显示,该细胞表达CD29间充质干细胞细胞表面标记物,低表达造血干细胞细胞表面标记物CD34;同时,该细胞诱导分化成脂肪细胞和成骨细胞实验表明,其具有多向分化潜能.结论:从比格犬会厌背侧黏膜分离的细胞具有间充质干细胞样特性,为进一步研究喉部瘢痕形成机制及喉部组织工程提供了技术基础. 相似文献
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本实验应用Nissl法、单胺荧光组织化学法和逆行荧光标记与单胺荧光组织化学结合技术对大白鼠中缝背核的组织结构及其向尾壳核(CP)复合体的定位投射进行了观察。结果表明,中缝背核(NRD)可分为五个细胞群:尾侧细胞群、背内侧细胞群、腹内侧细胞群、外侧细胞群和前侧细胞群;大量的5-羟色胺细胞分布于NRD的各个细胞群,少量儿茶酚胺(CA)细胞只见于外侧细胞区。在CP复合体注射逆行荧光化合物快兰(Fast Blue 253/50以下简称FB)之后,中缝背核内出现不少FB标记的5—HT细胞,这些投射于CP复合体的细胞主要位于背内侧细胞群和腹内侧细胞群。本实验结果为进一步研究NRD的功能提供了形态学依据。 相似文献
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目前,组织工程化血管的构建和工程化组织器官的血管化因内皮种子细胞的扩增能力不足和生物活性不强而受到限制。内皮祖细胞(EPC)是内皮细胞的前体细胞。出生后,EPC主要存在于骨髓,可向外周血液缓慢释放,参与机体缺血组织的血管重建和损伤血管的重新内皮化。现对EPC的来源、分布、表型特征、动员、分化、归巢、分离、培养与鉴定等生物学特性和EPC在组织工程中的应用进行了全面的综述,并指出目前存在的问题和研究方向。 相似文献
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Wang J Wang X Jiang S Lin P Zhang J Wu Y Xiong Z Ren JJ Yang H 《Cellular and molecular neurobiology》2008,28(7):991-1003
To provide suitable models for human GBM cancer stem cells in vitro and in vivo, and investigate their biological characteristics,
a new human GBM cancer stem-like cell line, WJ2, was established in this experiment through serial passages from adherent
monolayer culture to nonadherent tumor sphere culture in turns; Its partial biological characteristics were studied through
cell proliferation and tumor sphere assay; cell cycle distribution, side population, and CD133 phenotype were analyzed with
FCM. The expressions of CD133, Nestin, and GFAP of cancer stem-like cells and xenograft tumor cells were detected with RT-PCR
and immunohistochemistry. Biological characterization, side population, CD133 phenotype and CD133 Nestin, BCRP-1, Wnt-1 gene
expression revealed the stemness of this cancer stem-like cell line. Tumorigenicity heterotransplanted in nude mice; histopathological
characteristics of xenograft tumor, and expressions of CD133, Nestin, and GFAP of xenograft tumor cells indicated that xenograft
tumors recapitulated the phenotype and biological characterization of human primary GBM. All findings of this experimental
study suggested that WJ2 cancer stem-like cell line could accurately mimic human GBM cancer stem cell in vitro and in vivo; it would be useful in
the cellular and molecular studies as well as in testing novel therapies of CSC-based anti-cancer therapies for human GBM. 相似文献
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《Cell cycle (Georgetown, Tex.)》2013,12(20):2554-2562
The cancer stem cell theory describes a small subset of cancer cells that have the ability to initiate and drive the growth of a tumor. The niche refers to the environmental factors and the surrounding cells within which the tumor develops. The exact relationship between cancer stem cells and the tumor niche is not known. However, using side population analysis by flow cytometry, it is possible to analyze the relationship between environmental stresses and putative cancer stem cells. The side population is a subpopulation of cells that efflux Hoechst 33342 and has been previously shown to be enriched for cancer stem cells. Using this technique, we characterized the response of side population cells to low confluency, serum starvation and hypoxia using three different human cancer cell lines. We found that these stresses, characteristic of the tumor niche enrich the side population of DLD1, SW480 and MCF7 cancer cell lines, thus possibly predisposing the tumor to a more malignant phenotype. 相似文献
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ABCG2 is a member of the ATP binding cassette (ABC) transporters, which can pump a wide variety of endogenous and exogenous compounds out of cells. Widely expressed in stem cells, ABCG2 is also found to confer the side population phenotype and is recognized as a universal marker of stem cells. Although the precise physiological role of ABCG2 in stem cells is still unclear, existing data strongly suggest that ABCG2 plays an important role in promoting stem cell proliferation and the maintenance of the stem cell phenotype. In addition, ABCG2 is also found to be expressed in a number of cancer cells and appears to be a marker of cancer stem cells. Moreover, ABCG2 expression in tumors may contribute to their formation and progression. Thus, ABCG2 has potential applications in stem cell and tumor therapy. 相似文献
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Bin Huang Yi Jun Huang Zhi Jun Yao Xu Chen Sheng Jie Guo Xiao Peng Mao Dao Hu Wang Jun Xing Chen Shao Peng Qiu 《PloS one》2013,8(7)
Although cancers are widely considered to be maintained by stem cells, the existence of stem cells in renal cell carcinoma (RCC) has seldom been reported, in part due to the lack of unique surface markers. We here identified cancer stem cell-like cells with side population (SP) phenotype in five human RCC cell lines. Flow cytometry analysis revealed that 769P, a human clear cell RCC cell line, contained the largest amount of SP cells as compared with other four cell lines. These 769P SP cells possessed characteristics of proliferation, self-renewal, and differentiation, as well as strong resistance to chemotherapy and radiotherapy that were possibly related to the ABCB1 transporter. In vivo experiments with serial tumor transplantation in mice also showed that 769P SP cells formed tumors in NOD/SCID mice. Taken together, these results indicate that 769P SP cells have the properties of cancer stem cells, which may play important roles in tumorigenesis and therapy-resistance of RCC. 相似文献
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In recent years, a special type of cancer cell--the cancer stem cell (CSC)--has been identified and characterized for different tumors. CSCs may be responsible for the recurrence of a tumor following a primarily successful therapy and are thought to bear a high metastatic potential. For the development of efficient treatment strategies, the establishment of reliable methods for the identification and effective isolation of CSCs is imperative. Similar to their stem cell counterparts in bone marrow or small intestine, different cluster of differentiation surface antigens have been characterized, thus enabling researchers to identify them within the tumor bulk and to determine their degree of differentiation. In addition, functional properties characteristic of stem cells can be measured. Side population analysis is based on the stem cell-specific activity of certain ATP-binding cassette transporter proteins, which are able to transport fluorescent dyes out of the cells. Furthermore, the stem cell-specific presence of aldehyde dehydrogenase isoform 1 can be used for CSC labeling. However, the flow cytometric analysis of these CSC functional features requires specific technical adjustments. This review focuses on the principles and strategies of the flow cytometric analysis of CSCs and provides an overview of current protocols as well as technical requirements and pitfalls. A special focus is set on side population analysis and analysis of ALDH activity. Flow cytometry-based sorting principles and future flow cytometric applications for CSC analysis are also discussed. 相似文献
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Zou GM 《Journal of cellular physiology》2008,217(3):598-604
It has been suggested that cancer stem cells population within the solid tumor with indefinite proliferation potential drives the growth and metastasis of cancer. In literature, these malignant stem cells also named Cancer initiating cells. Cancer stem cells exhibit low rate of division and proliferation in their niche that help them to avoid chemotherapy and radiation. Epithelial cancers are believed to originate from transformation of tissue stem cells. Bone marrow-derived cells, which are frequently recruited to sites of tissue injury and inflammation, might also represent a potential source of malignancy in the gastrointestinal tract. Pancreatic cancer is one of most common cause of cancer-related death. Pancreatic cancer stem cells have been characterized recently through serial transplantation of human pancreatic cancer cells. The phenotype of Pancreatic cancer stem cells has been defined as CD24(+)CD44(+)CD326 (ESA)(+). CD133 antigen has been also suggested as a potential marker for cancer stem cell in gastrointestinal tract but recently there is also debate in this regard. More recently, other cancer stem cells in gastrointestinal tract, such as colon cancer stem cells, liver cancer stem cells, have been also characterized in their phenotype. These advances clearly will bring the new strategy in cancer treatment and control in the gastrointestinal tract. In this review, the author will discuss the current status and progress about cancer stem cell research in gastrointestinal tract and liver. 相似文献
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The side population (SP) phenotype is shared by stem cells in various tissues and species. Here we demonstrate SP cells with Hoechst dye efflux were surprisingly collected from the epithelia of both the rat limbus and central cornea, unlike in human and rabbit eyes. Our results show that rat limbal SP cells have a significantly higher expression of the stem cell markers ABCG2, nestin, and notch 1, compared to central corneal SP cells. Immunohistochemistry also revealed that ABCG2 and the epithelial stem/progenitor cell marker p63 were expressed only in basal limbal epithelial cells. These results demonstrate that ABCG2 expression is closely linked to the stem cell phenotype of SP cells. 相似文献
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Xu YiXin He ZhiYing Zhu HaiYing Chen XueSong Li JianXiu Zhang HongXia Pan XingHua Hu YiPing 《中国科学C辑(英文版)》2007,50(6):762-765
In the field of stem cell research, SP (side population) phenotype is used to define the property that cells maintain a high
efflux capability for some fluorescent dye, such as Hoechst 33342. Recently, many researches proposed that SP phenotype is
a phenotype shared by some stem cells and some progenitor cells, and that SP phenotype is regarded as a candidate purification
marker for stem cells. In this research, murine fertilized ova (including conjugate and single nucleus fertilized ova), 2-cell
stage and 8-cell stage blastomeres, morulas and blastocysts were isolated and directly stained by Hoechst 33342 dye. The results
show that fertilized ovum, blastomere and morula cells do not demonstrate any ability to efflux the dye. However, the inner
cell mass (ICM) cells of blastocyst exhibit SP phenotype, which is consistent with the result of embryonic stem cells (ESCs)
in vitro. These results indicate that the SP phenotype of ICM-derived ESCs is an intrinsic property and independent of the culture
condition in vitro, and that SP phenotype is one of the characteristics of at least some pluripotent stem cells, but is not shared by totipotent
stem cells. In addition, the result that the SP phenotype of ICM cells disappeared when the inhibitor verapamil was added
into medium implies that the SP phenotype is directly associated with ABCG2. These results suggest that not all the stem cells
demonstrate SP phenotype, and that SP phenotype might act as a purification marker for partial stem cells such as some pluripotent
embryonic stem cells and multipotent adult stem cells, but not for all stem cells exampled by the totipotent stem cells in
the very early stage of mouse embryos. 相似文献