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1.
外来入侵植物空心莲子草因其强抗逆性而迅速蔓延,成为有害杂草。拟研究其病程相关蛋白——几丁质酶酶学性质,为探讨该植物的抗逆机理和入侵机制提供理论依据。空心莲子草经乙烯利处理后,其叶片蛋白质提取液在4℃经40-60%饱和硫酸铵溶液沉淀,Sephadex G.75洗脱后得几丁质酶液。结果表明:空心莲子草几丁质酶最适反应pH为5.7左右,在pH4.0~8.0均表现稳定,当pH小于4.0和大于8.0时酶活力丧失50%以上,在pH2.0和10.0时没有活力;在30℃.80℃范围内,酶均具较高活性,最适反应温度为70℃左右,温度高于80℃后,酶稳定性下降;当底物浓度小于1.25%时,酶活力随底物浓度的增加而增加,在底物浓度大于1.25%浓度时,活力增加的趋势减缓。结果提示:也许空心莲子草的强抗逆性是因为其几丁质酶具较高的温度耐受性和较大的pH作用范围。  相似文献   

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荔枝果皮过氧化物酶的纯化及部分酶学性质研究   总被引:12,自引:0,他引:12  
经硫酸铵分级盐析、DEAE-Sepharose和Sephadex G-75柱层析分离,从荔枝果皮中分离提纯了过氧化物酶(POD),该酶被纯化了12.5倍,产率为1.9%。经SDS-PAGE确定为单一条带。该酶最适反应温度为35℃,对热具有较强的稳定性,经75℃处理30min,酶活性只损失50%。最适pH约为6.5,但在pH4.0—8.0范围内活力仍比较稳定。该酶在25℃和0.05mol/L磷酸缓冲液(pH7.0)条件下对愈创木酚、邻苯二酚和没食子酸的Km分别是2.75、12.4和12.8mmol/L。二硫苏糖醇和抗坏血酸能完全抑制POD活性,L-半胱氨酸、柠檬酸、FeS04、GSH、SDS和ZnS04对POD活性有一定的抑制作用,而FeCl,和CuSOt对POD则有较好的激活作用。  相似文献   

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陈爽  宋娜  廖学品  石碧 《生物工程学报》2011,27(7):1076-1081
将胶原纤维用三价铁改性后作为载体,通过戊二醛的交联作用将过氧化氢酶固定在该载体上。制备的固定化过氧化氢酶蛋白固载量为16.7 mg/g,酶活收率为35%。研究了固定化酶与自由酶的最适pH、最适温度、热稳定性、贮存稳定性及操作稳定性。结果表明:过氧化氢酶经此法固定化后,最适pH及最适温度与自由酶相同,分别为pH 7.0和25 ℃;但固定化酶的热稳定性显著提高,在75 ℃保存5 h后,仍能保留30%的活力,而自由酶则完全失活;固定化酶在室温下保存12 d后,酶活力仍保持在88%以上,而自由酶在此条件下则完全失  相似文献   

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高产天冬氨酸酶的大肠杆菌细胞的固定化   总被引:6,自引:1,他引:5  
用聚乙烯醇凝胶包埋具有高活力天冬氨酸酶的大肠杆菌(Escherichia coli)No.1细胞。该酶的表现活力高达1638 00u/g湿细胞,酶活力的回收率为97.5%。固定化细胞和游离细胞天冬氨酸酶的最适pH均为8.0,最适温度分别为40—45℃和40—55℃。二价金属离子Mn2+、Mg2+、Ca2+和Fe2+对热钝化的天冬氨酸酶活力具有保护作用。在37—45℃下,两种细胞的热稳定性相同。二者在pH6.0的柠檬酸缓冲液中比较稳定。固定化细胞在1mol/L、pH8.0的底物溶液(内含Mn2+1mmol/L)中于4℃冰箱保存6个月,天冬氨酸酶的活力保持不变。用固定化细胞柱连续生产L-天冬氨酸,底物转化为产物的转化率达95%以上;产物的总 收率为91.1%。固定化细胞柱连续运转40天,天冬氨酸酶活力仍保持最初酶活力的90%。  相似文献   

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分枝犁头霉菌丝体球经金刚砂磨碎用水提取的粗酶液,用两种方法纯化,一是用制备垂直平板凝胶电泳;另一是经硫酸铵分级沉淀、Sephadex G-200、DEAE-Sephadcx A50及羟基磷灰石等柱层析。这两种方法纯化后的样品经PACE鉴定皆为均一带,无其他糖苷酶活性。酶反应最适温室为55—60℃,在50℃保温7h,剩余活力为50%,70℃保温10min,则全部失活。最适pH为5.0,pH6.0一8.5稳定。  相似文献   

6.
温特曲霉延胡索酸酶的提纯及性质研究   总被引:1,自引:0,他引:1  
报道经硫酸鱼精蛋白沉淀、硫酸铵分级沉淀和葡聚糖凝胶G-200柱层析,再经冰冻干燥后从温特曲霉F-871菌体中获得延胡索酸酶,纯化倍数为31.70,回收率为36.64%,酶比活性为24.6U/mg。酶学性质研究表明:酶作用最适pH和温度分别为8.0和30℃,稳定pH范围为6.0~8.5,酶在35℃下保温30min后仍残留约90%以上的活力。  相似文献   

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谷胱甘肽硫转移酶(GST)的固定化及酶学特性研究   总被引:1,自引:0,他引:1  
蔡俊  邱雁临 《生物技术》2003,13(5):11-12
对谷胱甘肽硫转移酶的固定化、游离酶和固定化酶的酶学特性进行了研究,通过试验,确定谷胱甘肽硫转移酶的最佳固定化条件为先用2%壳聚糖吸附酶,然后再加戊二醛交联,交联用戊二醛浓度为1.2%,交联时间6h;游离酶的最适温度为45—55℃,最适pH值为6.5-7.0:固定化酶的最适温度为45-50℃,最适pH值为7.0;游离酶和固定化酶的最适酶促反应时间为30min。  相似文献   

8.
黄瓜几丁质酶的诱导,提取纯化及其基本性质(简报)   总被引:3,自引:0,他引:3  
3周龄黄瓜幼苗经乙烯利处理后,诱导了几丁质酶活力。叶片提取液经20%和60%饱和度的两步硫酸铵沉淀,通过再生几丁质亲和层析后,纯化制备的SDS-PAGE显示单一谱带。酶学特性呈现pH2.7和pH7.1两个最适反应pH和50℃的最适反应温度。纯化的酶对几种病原菌的生长有一定的抑制作用。  相似文献   

9.
固定化青霉素V酰化酶的制备及性质   总被引:2,自引:0,他引:2  
尖镰孢(Fusarium oxysporum)FP941青霉素V酰化酶经γ氧化铝吸附洗脱、硫酸铵沉淀和脱盐处理后,固定在环氧丙烯聚合物载体上,湿固定化酶表现活力为217 IU/g,固定化产率为53%。固定化酶作用最适温度为55℃,最适pH为80;在pH50~110及50℃以下稳定;37℃使用25次后,酶活力保留90%。  相似文献   

10.
固定化青霉素酰化酶的研究   总被引:11,自引:4,他引:7  
将巨大芽孢杆菌胞外青霉素酰化酶通过共价键连接到醋酸纤维素载体上,制成的固定化青霉素酰化酶的表观活力达2000 u/g左右(PDAB法)。水解lO%(w/v)的青霉素G钾盐落液,使用30批,保留活力70%以上。6-氨基青毒烷酸(6-APA)总收率平均达88.37%。固定化青霉素酰化酶水解青霉素G的最适pH为9.95,最适温度为55℃,表观米氏常数为1.093×10-2mol/L,在pH 5.8-10.7,温度45℃以下酶的活力稳定。  相似文献   

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Comprises species occurring mostly in subtidal habitats in tropical, subtropical and warm-temperate areas of the world. An analysis of the type species, V. spiralis (Sonder) Lamouroux ex J. Agardh, a species from Australia, establishes basic characters for distinguishing species in the genus. These characters are (1) branching patterns of thalli, (2) flat blades that may be spiralled on their axis, (3) width of the blade, (4) primary or secondary derivation of sterile and fertile branchlets and (5) position of sterile and fertile branchlets on the thalli. Application of the latter two characters provides an important basic method for separation of species into three major groups. Osmundaria , a genus known only in southern Australia, was studied in relation to Vidalia , and its separation from the Vidalia assemblage is not accepted. Species of Vidalia therefore are transferred to the older genus name, Osmundaria. Two new species, Osmundaria papenfussii and Osmundaria oliveae are described from Natal. Confusion in the usage of the epithet, Vidalia fimbriala Brown ex Turner has been clarified, and Vidalia gregaria Falkenberg, described as an epiphyte on Osmundaria pro/ifera Lamouroux, is revealed to be young branches of the host, Osmundaria prolifera.  相似文献   

16.
Fifteen chromosome counts of six Artemisia taxa and one species of each of the genera Brachanthemum, Hippolytia, Kaschgaria, Lepidolopsis and Turaniphytum are reported from Kazakhstan. Three of them are new reports, two are not consistent with previous counts and the remainder are confirmations of very scarce (one to four) earlier records. All the populations studied have the same basic chromosome number, x = 9, with ploidy levels ranging from 2x to 6x. Some correlations between ploidy level, morphological characters and distribution are noted.  相似文献   

17.
Curcumin is the yellow pigment of turmeric that interacts irreversibly forming an adduct with thioredoxin reductase (TrxR), an enzyme responsible for redox control of cell and defence against oxidative stress. Docking at both the active sites of TrxR was performed to compare the potency of three naturally occurring curcuminoids, namely curcumin, demethoxy curcumin and bis-demethoxy curcumin. Results show that active sites of TrxR occur at the junction of E and F chains. Volume and area of both cavities is predicted. It has been concluded by distance mapping of the most active conformations that Se atom of catalytic residue SeCYS498, is at a distance of 3.56 from C13 of demethoxy curcumin at the E chain active site, whereas C13 carbon atom forms adduct with Se atom of SeCys 498. We report that at least one methoxy group in curcuminoids is necessary for interation with catalytic residues of thioredoxin. Pharmacophore of both active sites of the TrxR receptor for curcumin and demethoxy curcumin molecules has been drawn and proposed for design and synthesis of most probable potent antiproliferative synthetic drugs.  相似文献   

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正Dear Editor,In December 2019, a novel human coronavirus caused an epidemic of severe pneumonia(Coronavirus Disease 2019,COVID-19) in Wuhan, Hubei, China(Wu et al. 2020; Zhu et al. 2020). So far, this virus has spread to all areas of China and even to other countries. The epidemic has caused 67,102 confirmed infections with 1526 fatal cases  相似文献   

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