中国青海省夕蚖属二新种记述(原尾纲,夕蚖科)

记述了采自青海省的夕蚖属2新种,即西宁夕蚖Hesperentomon ciningense sp.nov.和南山夕蚖Hesperentomon nanshanensis sp.nov.,两新种腹部第Ⅱ～Ⅵ节背板后排具有6对刚毛,毛序为8/12,第Ⅳ～Ⅵ节腹板缺失后排中刚毛Pc,毛序为4/8.西宁夕蚖和南山夕蚖相似,二者可以通过颚腺、雌性外生殖器、前足跗节的长度、感器形状以及腹部第Ⅹ节的毛序加以区分.新种模式标本保存在上海昆虫博物馆.     

中国东北原尾虫一新种记述(原尾纲,蚖科)

记述了采自辽宁省鞍山市千山的原尾虫1新种,千山线毛蚖Filientomon qianshanense sp. nov.,新种第Ⅱ、Ⅲ对腹足上各仅具1根刚毛,前足跗节末端的爪上具有内外悬片各1个.文中给出了线毛蚖属世界种类检索表.新种模式标本保存在上海昆虫博物馆.     

中国东北原尾虫一新属一新种(原尾纲,蚖科)

报道了采自我国东北地区辽宁省兴城市原尾虫1新属辽宁妧属Liaoxientulus gen.nov.及新种兴城辽宁妧Liaoxientulus xingchengensis sp.nov.和该属的1个未定名种Liaoxientulus sp.,新属属于蚖科屠妧亚科.新属与采自东北黑龙江省的屠妧亚科伊春蚖属Yichunentulus Yin,1980形态最为相近,其与后者主要差别在于下唇须和前跗内侧感觉器b′不同,伊春妧属下唇须有1根膨大的感觉器和4根刚毛,前跗内侧感觉器b′缺如;新属下唇须退化,其上感觉器缺如,刚毛只有3根,但是前跗内侧感觉器b′存在.新属现有2个种,均为此次新发现,其中1个种为新种Liaoxientulus xingchengensis sp.nov.,另外1个种为未定名种Liaoxientulus sp..新种正模1只和副模1只以及该属另1未定名种标本保存在中国科学院东北地理与农业生态研究所,新种另2只副模保存在上海植物生理生态所昆虫博物馆.     

中国原尾虫巴蚖属一新种(原尾纲,檗蚖科)

报道了采自我国东北地区吉林省长春市原尾虫1新种,长春巴蚖 Baculentulus changchunensis sp.nov..新种与采自朝鲜的 B.weinerae Szeptycki and Imadaté,1987以及采自日本的B.densus Imadaté,1960毛序相似,均为腹部Ⅰ～Ⅵ节背板Pla毛缺失.其与前者差别主要在于前跗感觉毛d位置、f和g长度、颚腺形状、栉梳形状和齿数,与后者差别主要体现在前跗感觉毛t-3大小、f和g末端位置、下唇须感觉毛形状和雌性生殖器.新种模式标本保存在中国科学院上海植物生理生态所昆虫博物馆.     

原尾目昆虫的两新属

本文记述自1963年到1973年从上海佘山和苏州等地采到的2个新属、3个新种和2个新组合,其中一新属隶于夕蚖科,另一属属于古蚖科,现分别讨论如下。 1.夕蚖科一新属和对该科的简评 沪蚖属 Huhentomon Yin 新属 模式种:褶爪沪蚖 Huhentomon plicatunguis 新种。 第Ⅰ腹足2节,第Ⅱ—Ⅲ腹足均为1节;假眼长卵形,具有长而宽的中裂;下颚腺细长如管;腹部Ⅳ-Ⅶ节腹板后排刚毛均有中央毛,第Ⅷ节腹板生2排刚毛共6根;口器、前胸足跗节感觉刚毛以及背、腹部刚毛式均与夕蚖属同型;外生殖器与蚖属者同型。     

广西地区古蚖属九新种

本文记述从广西桂林、南宁和武鸣等地采得的原尾虫,其中隶属于古蚖属(Eosentomon)的,共有九个新种,分述如下。模式标本保存中于国科学院上海昆虫研究所。1.巨形古蚖Eosentomon magnum,新种(图1～8) 体长1480～1670微米,体壁骨化较强,前跗和腹部末端均呈黄褐色,头长156～169微米,宽110～121微米,具上唇刚毛,唇基内骨呈马蹄形。假眼较大,16～17×16微米,具五条纵行线纹,其中双数线纹(2.4位)较粗,单数线纹(1、3、5位)的前半段纤细,后半段较粗;在前后两段线纹交界处,各生一小圆球,如图2所示,这一特征过去尚未见有报导。头眼比=9～10。     

中国云南齿甲属分类研究(鞘翅目,拟步甲科,齿甲族)

对中国云南省的齿甲属Uloma Dejean进行了分类整理,描述1新种Uloma valgipes sp.nov.并绘图,新种与小齿甲U.minuta Liu,Ren& Wang,2007相似,但可以通过以下特征区别于后者:前足胫节向内侧极弯;雄性前胸背板无凹;前胸背板前缘近两侧具细饰边,中部1/3范围内无饰边;后足第1跗节长于第4节;雄性外生殖器形状不同.并给出了云南省已知种类检索表.模式标本保存于河北大学博物馆.弯胫齿甲,新种Ulomavalgipes sp.nov.(图1～9)正模♂,云南龙陵龙新黑山(海拔2 300 m),2008-07-23,徐吉山采.词源:新种种名源自拉丁词valgus(弯的)+拉丁词pes (足)变化词尾组合而成,意指该虫的雄性前足胫节向内侧极弯曲.     

中国沙泥蜂亚科一新纪录属及一新种记述(膜翅目,泥蜂科)

记述了泥蜂科沙泥蜂亚科Ammophilinae中国1新纪录属:异足沙泥蜂属Parapsammophila Taschenberg,1869,及该属的1个新种,无脊异足沙泥蜂Parapsammophila vecarinata sp.nov..新种与P.foleyi(de Beaumont)相似,但前者雌性的唇基前缘中部具凹缺,唇基中部无中纵脊;中胸腹板前面正常;前足跗节不对称性较后者强;中足胫节具1距;上颚基半部、唇基前部、触角柄节、鞭节Ⅰ节基部内侧、3对足除基节基部外、前胸背板侧叶、翅基片、腹部除背板Ⅰ基部外为红黄色;雄性触角鞭节Ⅶ～X节内侧为暗红色;外生殖器阳茎端突外缘无齿等特征与后者明显有别.模式标本均采自我国新疆.模式标本保存在云南农业大学昆虫标本室.     

厚绥螨属一新种和我国一新纪录(蜱螨目:厚厉螨科)

厚绥螨属Pachyseius是Berlese于1910年建立的。该属的主要特征为:背板一块,上有简单刚毛30对。胸板具3对刚毛,第四对刚毛位于胸后板上。腹肛板紧邻后缘平直的生殖板,其后缘和后侧缘不与背板愈合。腹肛板具肛前毛2—4对,肛侧毛位于肛门之前。气门板狭窄。足后板细长。须肢跗节具三叉刚毛。足Ⅱ跗节具刺和棘。     

拟科蚱属的分类研究及一新种记述（直翅目： 短翼蚱科）

拟科蚱属Cotysoides建立于2000年, 该属的主要特征为体小型; 头顶前缘突出于复眼之前; 触角丝状, 着生于复眼前缘下1/3处; 前胸背板背面密具小瘤突; 中隆线波状, 形成一连串小突起; 后突不超过后足股节顶端; 前胸背板侧片外翻, 后角顶平截; 后翅不达后突顶端; 后足跗节第1节长于第3节。本文记述拟科蚱属6种, 包括1新种, 即断隆拟科蚱Cotysoides interruptus sp. nov. 该新种近似于广西拟科蚱Cotysoides guangxiensis Zheng et Jiang, 2000, 主要区别为： 1）头顶为一眼宽的1.5倍; 2）前胸背板侧片前角钝角形; 3）后翅伸达后足股节膝部; 4）中足股节宽狭于前翅宽; 5）雌性下生殖板后缘中央三角形突出。附有分种检索表、 分布及文献引证。新种的模式标本保存于陕西师范大学动物研究所及西南林业大学博物馆。     

TP53 and p16INK4A, but not H-KI-Ras, are involved in tumorigenesis and progression of pleomorphic adenomas

The putative role of TP53 and p16(INK4A) tumor suppressor genes and Ras oncogenes in the development and progression of salivary gland neoplasias was studied in 28 cases of pleomorphic adenomas (PA), 4 cases of cystic adenocarcinomas, and 1 case of carcinoma ex-PA. Genetic and epigenetic alterations in the above genes were analyzed by Polymerase Chain Reaction/Single Strand Conformational Polymorphism (PCR/SSCP) and sequencing and by Methylation Specific-PCR (MS-PCR). Mutations in TP53 were found in 14% (4/28) of PAs and in 60% (3/5) of carcinomas. Mutations in H-Ras and K-Ras were identified in 4% (1/28) and 7% (2/28) of PAs, respectively. Only 20% (1/5) of carcinomas screened displayed mutations in K-Ras. p16(INK4A) promoter hypermethylation was found in 14% (4/28) of PAs and 100% (5/5) carcinomas. All genetic and epigenetic alterations were detected exclusively in the epithelial and transitional tumor components, and were absent in the mesenchymal parts. Our analysis suggests that TP53 mutations and p16(INK4A) promoter methylation, but not alterations in the H-Ras and K-Ras genes, might be involved in the malignant progression of PA into carcinoma.     

Cloning, characterization and expression analysis of two Tetraodon nigroviridis interleukin-16 isoform genes

Interleukin-16 (IL-16) is an important pro-inflammatory cytokine that functions as a chemoattractant factor and is well characterized in human and other mammals, but is largely unknown in fish. In the present study, two isoforms of pro-IL-16 homologues were cloned and characterized from pufferfish Tetraodon nigroviridis. The full-length T. nigroviridis pro-IL-16 isoform 1 cDNA exhibits 2453 bp in size including 291 bp 5'UTR (untranslated region), 1704 bp ORF (open reading frame) and 458 bp 3'UTR, while pro-IL-16 isoform 2 cDNA exhibits a 3801 bp ORF and a 458 bp 3'UTR. Bioinformatics analysis demonstrated that the pro-IL-16 isoform 1 with a predicted mass of 60.6 kDa contained two PDZ (postsynaptic density/disc large/zona occludens-1) domains, whereas the 138.2 kDa pro-IL-16 isoform 2 had two additional PDZ domains in its N-terminal extension. RT-PCR results revealed that ,almost in all examined organs and tissues, the mRNA of both pro-IL-16 isoforms can be detected, except in intestine and gill, where the isoform 2 mRNA is absent. The two putative precursor proteins showed 30.0-33.0% identity to various mammalian and avian homologues. This is the first report of such genes in teleostean fish and we hope the molecular characterization of these two pro-IL-16 isoforms will provide insights into the study of both evolution of IL-16 precursor proteins and the immune system as a whole.     

Three new species of Eimeria (Apicomplexa: Eimeriidae) from Apalone spinifera pallidus (Testudines: Trionychidae) in Texas, with a redescription of E. amydae

Three new species of Eimeria are described from pallid spiny softshells, Apalone spinifera pallidus, collected in north-central Texas. Oocysts of Eimeria spinifera n. sp. were found in the feces of 3/9 (33%) turtles and are subspheroid, ellipsoid, or pear-shaped, 16.3 x 14.0 (14-19 x 12-18) microns, with a thin, single-layered wall; shape index 1.2 (1.1-1.3). A micropyle is absent, but an oocyst residuum is present; polar granule present in 16% of the oocysts. Sporocysts are elongate-ovoid, 10.3 x 5.2 (8-12 x 5-6) microns, each with a Stieda body bearing short filaments. Oocysts of Eimeria apalone n. sp. were found in 5/9 (56%) turtles and are ellipsoid, elongate pear-shaped, or subspheroid, 16.8 x 13.2 (12-19 x 10-16) microns, with a thin, single-layered wall; shape index 1.3 (1.0-1.5). A micropyle, oocyst residuum, and polar granule are absent. Sporocysts are elongate-ovoid, 11.3 x 6.2 (9-14 x 5-7) microns, each with a prominent Stieda body. Oocysts of Eimeria pallidus n. sp. were found in 4/9 (44%) A. s. pallidus and are spheroid or subspheroid, 23.4 x 21.6 (18-27 x 17-25) microns, with a thin, single-layered wall; shape index 1.1 (1.0-1.3). A micropyle is absent, but an oocyst residuum is present; polar granule present in 20% of the oocysts. Sporocysts are elongate-ovoid, 14.3 x 6.2 (13-17 x 6-7) microns, each with a Stieda body and short filaments. In addition to the new species, 3 previously described eimerians, including Eimeria amydae Roudabush, 1937, which is redescribed, were also found.     

A novel synthetic peptide vector system for optimal gene delivery to bone marrow stromal cells.

A 23-amino acid, bifunctional, integrin-targeted synthetic peptide was evaluated for ex vivo gene delivery to rabbit bone marrow stromal cells (BMSCs). The peptide (K)(16)GRGDSPC consists of an amino terminal domain of 16 lysines for electrostatic binding of DNA, and a 7-amino acid integrin-binding domain at the carboxyl terminal. PcDNA3-EGFP plasmids were transfected into BMSCs by (K)(16)GRGDSPC and the positive cells gave out a bright green fluorescence. High levels of gene delivery of pcDNA3-TGF-beta1 plasmids were obtained with 2 to 4 microg/ml DNA concentration, with (K)(16)GRGDSPC at an optimal peptide: DNA w/w ratio of 3:1, with a required exposure time of more than 4 h but shorter than 24 h for BMSC exposure to the peptide/DNA complexes with completely absent serum in the initial stage; with 100 microM chloroquine and at least 8 h exposure for BMSC exposure to chloroquine; with a fusogenic peptide at an optimal (K)(16)GRGDSPC/DNA/fusogenic peptide w/w ratio of 3:1:5; and with Lipofectamine 2000 at an optimal (K)(16)GRGDSPC/DNA/Lipofectamine 2000 w/w ratio of 3:1:2 at a constant DNA concentration of 2 microg/ml. Chloroquine, the fusogenic peptide and Lipofectamine 2000 all significantly promoted gene delivery, but chloroquine was more effective than the fusogenic peptide and had obvious synergistic effects with Lipofectamine 2000. Under optimal conditions, TGF-beta1 gene was transfected into BMSCs without observable toxicity, and the stable expression was examined by RT-PCR and Western blot analysis. The stable transgenic cells showed obvious bands. This novel synthetic peptide, providing a new way for the use of polylysine and RGD motif in DNA vector system, is potentially well suited to ex vivo gene delivery to BMSCs for experimental and clinical applications in the field of bone tissue engineering.     

Methylation of the putative tumor suppressor gene, RASSF1A, in primary cervical tumors

The methylation level of 13 CpG-dinucleotides in the promoter region of the putative tumor suppressor gene RASSF1A (3p21.31) was analyzed in HPV-positive squamous cell carcinomas of cervix using methyl-sensitive restriction endonuclease analysis followed by PCR. The methylation from 3 to 13 CpG-dinucleotides was observed in 64% (25/39) tumors, 22% (2/9) morphologically normal tissues adjacent to tumors (P = 0.0306) and in 2 from 3 leucocytes of peripheral blood of patients. The methylation of these CpG-dinucleotides was absent in DNA of healthy donor leucocytes (0/10). Methylation level of the examined fragment of the RASSF1A promoter region was significantly higher in tumors of patients with lymph node metastases in comparison to tumors of patients without metastases (P = 8.5 x 10(-12)). The methylation frequency of RASSF1A gene was in two times higher than hemi- and homozygous deletion frequency at the region of location of this gene (chromosome 3p21.31), determined earlier. These data suggest that methylation of the RASSF1A gene is one of the main ways of this gene inactivation in HPV-positive cervical squamous cell carcinomas. The methylation of the RASSF1A gene is an early event in genesis of tumor and the level of methylation increased with tumor progression.     

Palmitoleate Is a Mitogen, Formed upon Stimulation with Growth Factors, and Converted to Palmitoleoyl-phosphatidylinositol

Controversial correlations between biological activity and concentration of the novel lipokine palmitoleate (9Z-hexadecenoate, 16:1) might depend on the formation of an active 16:1 metabolite. For its identification, we analyzed the glycerophospholipid composition of mouse Swiss 3T3 fibroblasts in response to 16:1 using LC-MS/MS. 16:1 was either supplemented to the cell culture medium or endogenously formed when cells were stimulated with insulin or growth factors as suggested by the enhanced mRNA expression of 16:1-biosynthetic enzymes. The proportion of 1-acyl-2-16:1-sn-phosphatidylinositol (16:1-PI) was time-dependently and specifically increased relative to other glycerophospholipids under both conditions and correlated with the proliferation of fatty acid (16:1, palmitate, oleate, or arachidonate)-supplemented cells. Accordingly, cell proliferation was impaired by blocking 16:1 biosynthesis using the selective stearoyl-CoA desaturase-1 inhibitor CAY10566 and restored by supplementation of 16:1. The accumulation of 16:1-PI occurred throughout cellular compartments and within diverse mouse cell lines (Swiss 3T3, NIH-3T3, and 3T3-L1 cells). To elucidate further whether 16:1-PI is formed through the de novo or remodeling pathway of PI biosynthesis, phosphatidate levels and lyso-PI-acyltransferase activities were analyzed as respective markers. The proportion of 16:1-phosphatidate was significantly increased by insulin and growth factors, whereas lyso-PI-acyltransferases showed negligible activity for 16:1-coenzyme A. The relevance of the de novo pathway for 16:1-PI biosynthesis is supported further by the comparable incorporation rate of deuterium-labeled 16:1 and tritium-labeled inositol into PI for growth factor-stimulated cells. In conclusion, we identified 16:1 or 16:1-PI as mitogen whose biosynthesis is induced by growth factors.     

16-sulfates of estriol in body fluids of human pregnancy at term.

A method was developed for the assay of estriol-16-sulfate (E3-16S) and estriol-3, 16-disulfate (E3-3,16-diS) in maternal serum, cord serum and amniotic fluid at delivery in human pregnancy. Tritiated E3-16S and E3-3,16-diS are added to the fluid being analyzed. The conjugates are separated and purified by sequential chromatography on alumina, Celite and Sephadex LH-20. Each conjugate is hydrolyzed with Glusulase and the released estriol is quantified by radioimmmunoassay. E3-3,16-diS was found in each fluid, most concentrated in the cord serum. Small amounts of E3-16S were found in some amniotic fluids, and this conjugate was virtually absent from the sera. These new estriol conjugates comprise less than 1 percent of total, estriol, apparently too low to be of diagnostic value in human pregnancy.     

内蒙古二连盆地努和廷勃尔和剖面阿山头组底部鼠齿类一新属(英文)

努和廷勃尔和剖面位于内蒙古二连市西南40 km的呼和勃尔和地区,依据沉积间断可以将50 m厚的地层分为脑木根组和阿山头组,地层时代从中古新世到中始新世。在阿山头组下部层位中发现大量的啮齿类化石,其中一类原始的鼠齿类在此被命名为一个新的属种:Erlianomys combinatusgen. et sp. nov. (综合二连鼠)。其主要牙齿特征为:齿冠低,主尖较为发育,连接各尖的脊简单、细弱。有P4, m1有前压痕也表明有一个小的p4或者dp4。M1和M2大小相当。臼齿前齿带(下前齿带)明显,与原尖(下原尖)之间没有连接或连接很弱。M1和M2原尖后臂、后脊和次尖前臂在中尖处相交。m1的下前尖很弱或缺失,下原尖与下后尖之间连接很弱,基本为孤立的两个尖;m2 -3下次小尖明显,下次脊短,有时直接与下次小尖相连;下外脊低矮、不发育。上、下臼齿都没有中脊或很弱。新属种的发现,为进一步认识古近纪啮齿类的起源和演化提供了新的证据。Erlianomys与北美的Elymys和亚洲的Aksyiromys,Primisminthus,Allosminthus,Palasiomys都有很多相似的特征,预示着它们可能有共同的祖先。在牙齿形态上,Erlianomys比中始新世的其他鼠齿类更为原始,可表明其产出层位即阿山头组下部的时代要早于中始新世,可能属于早始新世;其形态更接近亚洲的Aksyiromys,Primisminthus和Allosminthus,而与北美的Ely-mys相差较大。因此推测鼠齿类的共同祖先可能与Erlianomys更为相似,早始新世时在亚洲起源,向其他大陆的迁移扩散不会晚于早始新世晚期。     

Biochemical and Genetic Polymorphisms for Carboxylesterase and Acetylesterase in Grape Clones of Vitis vinifera L. (Vitaceae) Cultivars

Native polyacrylamide gel electrophoresis (PAGE) was employed to show the highest number of esterase loci and to detect alpha- and beta-esterase polymorphisms in leaf buds of Vitis vinifera cultivars. A total of 16 esterase isozymes were detected in leaf buds from 235 plants including Italia, Rubi, Benitaka, and Brasil cultivars. Biochemical characterization of the grape esterases using ester substrates revealed alpha-, beta-, and alpha/beta-esterases with inhibitor tests distinguishing both carboxylesterases (EST-2, EST-3, EST-5, EST-6, EST-7, EST-8, EST-9, EST-10, and EST-16 isozymes) and acetylesterases (EST-4, EST-11, EST-12, EST-13, EST-14, EST-15 isozymes). No allele variation for alpha-, beta-, and alpha/beta-esterases was detected; however, EST-3 alpha-carboxylesterase was absent in 61.7% of vines, and EST-4 alpha/beta-acetylesterase was absent in one vine of Rubi cv. Null EST-3 carboxylesterase phenotype (61.7%) cannot be explained in this article, but the high genetic polymorphism in four V. vinifera clones is a positive aspect for genetic selection and development of new clones with different characteristics.