首页 | 本学科首页   官方微博 | 高级检索  
相似文献
 共查询到20条相似文献,搜索用时 78 毫秒
1.
粟米草的组织培养和快速繁殖   总被引:1,自引:0,他引:1  
1 植物名称 粟米草(Mollugo pentaphrlla)。 2 材料类别叶、带腋芽茎段。 3 培养条件诱导愈伤组织培养基:(1)MS+ 2,4-D 1 mg·L-1(单位下同)+6-BA 1; (2) MS + NAA 1 + 6-BA 0.5; (3)MS + 2,4-D2+ KT 0.1; (4)MS + IBA 1 + 6-BA 2。诱导芽分化培养基:(5)MS + ZT 2+ NAA 0.2+ CH500;(6) MS + ZT 4 + NAA 0.5 + CH 500; (7)MS + 6-BA 2 + NAA 0.5 + CH 500; (8)MS + KT1 +NAA 0.1。 诱导生根培养基:(9)MS+NAA1 +6-BA 0.1; (10)MS + NAA 0.5 + KT 0.5。每种培养基均附加3%蔗糖,0.7%琼脂,pH5.8。培养温度(25±1)℃,光照每天12 h,照度2 000 lx左右。 4 生长与分化情况 4.1 无菌材料的获得 将粟米草种子用70%乙醇浸泡1min,自来水冲洗,再用0.1%升汞灭菌10min,在超净台上用无菌水漂洗4次,接种于MS固体培养基上。待苗长至4cm高时,剪其叶和带腋芽茎段作外植体。  相似文献   

2.
中国栗米草属植物的校订   总被引:1,自引:0,他引:1  
鲁德全 《植物研究》1993,13(2):110-112
本文校订了中国粟米属植物,共有4种。国产的粟米草应是Mollugo stricta L.,而不是M.pentaphylla L.;多棱粟米草M.costataY.T.Chang et C.F.Wei是种棱粟米草M.verticillata L.的异名  相似文献   

3.
毛蕊花和粟米草毛状根培养系统的建立(简报)   总被引:6,自引:1,他引:5  
用发根农杆菌A4、R1601、ATCC15834从毛蕊花和粟米草的无菌子叶中诱导出毛状根,经高压纸电泳鉴定,这两种毛状根中均含有甘露碱,并测定了它们的生长曲线。  相似文献   

4.
国产粟米草属的分类研究   总被引:2,自引:0,他引:2  
本文根据国产粟米草属Mollugo L.8种植物的外部形态的5个分类学特征,尤其是种子形态结构的演化特征,认为该属植物种中实际存在着两个自然类群,特将该属中种子具有假种皮和种阜的、即星粟草属Glinus L.在我国予以恢复,它是一个较粟米草属原始的自然类群。  相似文献   

5.
该文报道了发现于北京市密云区的1种被子植物新记录——荨麻叶龙头草[Meehania urticifolia(Miq.)Makino],其所隶属的龙头草属(Meehania Britton)为北京分布新记录。描述了荨麻叶龙头草的形态特征与生境,凭证标本藏于浙江大学标本馆(HZU)。荨麻叶龙头草于1899年首次发现于日本,是东北亚分布植物,此前在中国仅分布于东北。北京云蒙山发现的分布点,显然是该种目前在中国分布最西的分布点,可能于冰川期从东北亚迁移而来。  相似文献   

6.
1999年6月至2001年4月调查表明,重庆市分布(树)麻雀、山麻雀两种。前者分布于20个区(县),后者分布于17个区(县);有13个区(县)两种都有分布。分布特征为:①分布广,②垂直分布的最适海拔高度为500~1000m,③水平分布西部多于东部,④分布与人类最亲近,有人烟的地方就有麻雀分布。可见麻雀是各类型生态系统中的重要一员。  相似文献   

7.
李后魂  郑乐怡 《昆虫学报》1999,42(4):411-417
报道了鞘蛾科 (Coleophoridae) 脉鞘蛾组 (Colephora follicularis group) 5个分布于中国的种, 其中有3个新种和2个中国新记录种:矛尖脉鞘蛾(新种)Coleophra jaculatoria sp. nov. 分布于陕西(周至);奇脉鞘蛾Coleophora frankii Schmidt新记录于新疆(巩留),国外分布奥地利,捷克,斯洛伐克,匈牙利,南斯拉夫,德国和意大利;隐脉鞘蛾 Coleophora pseudociconiella Toll 新记录于新疆(塔城),国外分布奥地利,意大利,捷克和土耳其;四叉脉鞘蛾(新种) Coleophora quadrifurca sp. nov.分布陕西(杨陵、澄城);榆中脉鞘蛾(新种)Coleophora yuzhongensis sp. Nov. 分布甘肃(榆中)。文中提供了分种检索表和新种的外生殖器特征图。模式标本保存在南开大学生物系。  相似文献   

8.
李后魂  郑乐怡 《昆虫学报》2000,43(2):188-192
该文记载了尖鞘蛾组(Coleophora absinthii group)7个分布于中国的种,其中有1个新种和4个中国新记录种:蒿尖鞘蛾Coleophora absinthii Heinemann et Wocke 新记录于新疆(巩留、北屯)和黑龙江(哈尔滨、黑河、嫩江、五大连池),国外分布欧洲;沟尖鞘蛾Coleophora amarchana Falkovitsh新记录于青海(湟源),国外分布蒙古;野艾尖鞘蛾Coleophora artemisicolella Bruand 新记录于黑龙江(嫩江),国外分布日本和欧洲;凤县尖鞘蛾(新种)Coleophora fengxianica sp.nov.分布陕西(凤县),该种与蒿尖鞘蛾很相似,但雄性外生殖器的抱器背基突更窄、抱器小瓣和抱器腹更宽、抱器腹背突更长、角状器强烈弯曲以及阳茎端环棒的形状不同可以和后者相区别;平须尖鞘蛾Coleophora kudrosella Baldizzone et Oku 新记录于新疆(乌鲁木齐、北屯、精河),内蒙古(锡林浩特)和黑龙江(嫩江),国外分布日本;茼蒿尖鞘蛾Coleophora kurokoi Oku分布云南(丽江),国外分布日本;钝尖鞘蛾Coleophora yomogiella Oku 分布安徽(岳西),黑龙江(黑河),云南(丽江),国外分布韩国和日本。文中提供了国内已知种的分种检索表和新种的外生殖器特征图。模式标本保存在南开大学生物系。  相似文献   

9.
两种爬行动物胃肠道内分泌细胞的免疫组织化学比较研究   总被引:23,自引:6,他引:17  
目的研究七种胃肠激素内分泌细胞在多疣壁虎及石龙子胃肠道的定位及分布。应用S-P免疫组织化学方法。方法结果5-羟色胺(5-Hydroxytryptamine,5-HT)细胞在两种爬行动物胃肠道各段均有分布,但分布型各不相同;生长抑素(Somatostatin,SS)细胞仅在多疣壁虎胃贲门至十二指肠段分布,而石龙子胃肠道各段均有分布;胃泌素(Gastrin,Gas)细胞分布于多疣壁虎十二指肠和空肠段,石龙子则仅分布于胃幽门部;胰高血糖素(Glucagon,Glu)细胞和P-物质(Substance P,SP)细胞均分布于多疣壁虎胃中各段,而石龙子胰高血糖素细胞分布于回肠、直肠及胃体处,P-物质细胞则分布于除空肠外的胃肠道各段;胰岛素(Insulin,Ins)细胞和胰多肽(Pancreatic polypeptide,PP)细胞在两种爬行动物胃肠道各段均未检出。结论两种爬行动物胃肠道内分泌细胞的分布既存在着一定共性,体现了两消化生理的共同点;同时也存在着较大的种间差异。  相似文献   

10.
蒲富基 《昆虫学报》1991,34(3):341-343
小扁天牛属(Callidiellum)隶属于天牛亚科(Cerambycinae)。1940年美国Linsley以Semanotus cupressi Van Dyke 1923为模式种建立小扁天牛属,全球已知五种(包括本文新种在内),即C.cupressi(Van Dyke)1923(分布于美国加州地区),C.virescens Chemsak et Linsley 1966(分布于美国亚利桑那州),C.rufipennis(Motschulsky)1860(分布于日本,朝鲜及中国,C.villosulum(Fairmaire)1900(广布于中国南  相似文献   

11.
A comparison between Ca and Sr cycling in forest ecosystems   总被引:8,自引:1,他引:7  
Poszwa  Anne  Dambrine  Etienne  Pollier  Benoît  Atteia  Olivier 《Plant and Soil》2000,225(1-2):299-310
In favourable conditions, the 87Sr/86Sr isotope ratios of the Sr delivered by rain and soil mineral weathering differ. Assuming that Ca and Sr behave similarly in forest ecosystems, several authors have used the 87Sr/86Sr variation in forest compartments to calculate the contribution of rain and mineral weathering to Ca fluxes and pools. However, there are a number of experimental reports showing that Ca and Sr may behave differently in the soil and in the plant. We have tested this Ca–Sr analogy in the field by measuring the variation of Sr and Ca concentrations, fluxes and pools in spruce, beech and maple stands on granite, sandstone and limestone. Results show that (1) variations of Ca and Sr concentrations are generally correlated at each level of the ecosystems. (2) In spruce on acid soils, a preferential uptake of Ca over Sr occurs (Aubure spruce Sr/Ca = 0.8×10−3; soil exchangeable Sr/Ca between 2 and 6×10−3). On calcareous soils, a preferential uptake of Sr over Ca by spruce may occur. (3) In spruce and beech on acid and calcareous soils, a preferential translocation of Ca over Sr from roots to leaves occurs ((Sr/Ca) in leaves was between 10 and 90% of that in roots). (4) The biological cycling of Ca and Sr leads to an enrichment of the upper soil layers in Ca and Sr. Compared to Sr, Ca accumulates in the upper layer of acid soils because Ca cycling through litterfall is favoured over Sr cycling, and possibly because of the selectivity of acid organic exchangers for Ca. This revised version was published online in June 2006 with corrections to the Cover Date.  相似文献   

12.
报道了内蒙古1个新分布记录属:粟草属(Milium L.);2个新分布记录种:翅柄车前(Plantago komaroviiPavl.)和粟草(Milium effusum L.)。  相似文献   

13.
The effect of iron supply on the growth and nodulation ofLupinus angustifolius L. (Gungurru),Lupinus luteus L. (R-1171) andLupinus pilosus Murr. (P20957) was studied in acid solutions. Plants of the three species were grown together in the same solution and inoculated withBradyrhizobium (Lupinus) WU 425. Plants were then grown with or without applied NH4NO3. The lupin species differed greatly in their sensitivity to low iron concentrations in solution withL. pilosus being most tolerant andL. luteus most sensitive.L. pilosus had the highest iron concentration in tissues and had a higher ratio of iron concentration in the youngest fully expanded leaf blades (YEB) to that in roots than the other two species.L. luteus had higher iron concentrations in roots but lower iron concentration in YEB and shoots than didL. angustifolius. The requirements of internal iron for the maximal chlorophyll synthesis in YEB were 65 μg g-1 forL. angustifolius andL. luteus, and 52 μg g-1 forL. pilosus. In contrast to effects on growth, the three species had similar external iron requirements for nodule formation in roots and for maximal nitrogen concentrations in shoots. The results indicate that iron tolerant lupin species require lower internal and external iron supply and have a greater ability than sensitive species to translocate iron from roots to shoots.  相似文献   

14.
Cytochemical investigations on peroxidase localization during microsporogenesis inLarix europaea D.C.,Taxus baccata L. andPinus sylvestris L. have revealed striking differences in the localization and activity level of this enzyme linked with the developmental stage. The localization and level of activity of peroxidase, typical of each stage, changed in the course of microsporogenesis in a strictly orderly way, giving a characteristic and stable pattern. The pattern of intracellular peroxidase localization proved to be the same for microsporogenesis of all the gymnosperms in question. It is suggested that the identity of that pattern in plants so phylogenetically distant asTaxus baccata L. andPinus sylvestris L. indicates that peroxidase activity in gymnosperms’ microsporogenesis is connected with the fundamental and genetically well stabilized processes of meiotic cytodifferentiation. Moreover, enhanced peroxidase activity has been found in the sites of callose walls synthesis of dyads and tetrads, which suggests the participation of this enzyme in callose synthesis.  相似文献   

15.
Based on the data obtained in the experiments with constant and alternating diurnal temperatures, the temperature dependence of developmental rate was calculated for different photoperiodic groups of plants using the square regression equations. These equations made it possible to calculate the temperature regions of maximum developmental rates of plants under different photoperiods.  相似文献   

16.
Apical meristems from adventitious buds induced by culturing of bulb-scale segments of Japanese Pink Lily (Lilium japonicum Thunb.) were successfully cryopreserved by a vitrification. The excised apical meristems were precultured on a solidified Murashige & Skoog medium, containing 0.3 M sucrose, for 1 day at 25°C and then loaded in a mixture of 2 M glycerol plus 0.4 M sucrose for 20 min at 25°C. Cryoprotected meristems were then sufficiently dehydrated with a highly concentrated vitrification solution (designated PVS2) at 25°C for 20 min or at 0°C for 110 min prior to a plunge into liquid nitrogen. After rapid warming in a water bath at 40°C, the meristems were placed in 1.8 ml of 1.2 M sucrose for 20 min and then, placed on filter papers over gellan gum-solidified MS medium. The revived meristems resumed growth within 5 days and directly produced shoots. The rate of shoot formation was approximately 80% after 4 weeks. When bulb-scale segments with adventitious buds were cold-hardened at 0°C for more than 7 days before the procedure, the rates of shoot formation were significantly increased. This vitrification method was successfully applied to five other lily cultivars. Thus, this vitrification procedure for cryopreservation appears promising as a routine method for cryopreserving meristems of lily.Abbreviations DMSO dimethylsulfoxide - EG ethylene glycol - LN liquid nitrogen - MS medium Murashige & Skoog (1962) medium - PVS2 vitrification solution  相似文献   

17.
RNA-degrading enzymes play an important role in regulating gene expression, and sequence analyses have revealed significant homology among several plant RNA-degrading enzymes. In this study we surveyed crude extracts of the above-ground part of the common wheat (Triticum aestivum L.) and the cultivated barley (Hordeum vulgare L.) for major RNA-degrading enzymes using a substrate-based SDS-PAGE assay. Fifteen wheat and fourteen barley RNA-degrading enzymes, with apparent molecular masses ranging from 16.3 to 40.1 kD, were identified. These RNA-degrading enzymes were characterized by their response to pH changes and addition of EDTA and ZnCl2 to the preincubation or incubation buffers. The 33.2- to 40.1-kD wheat and barley, 31.7-kD wheat, and 32.0-kD barley enzyme activities were inhibited by both zinc and EDTA and were relatively tolerant to alkaline environment. The 22.7- to 28.2-kD enzymes were inhibited by zinc but stimulated by EDTA. The 18.8-kD enzyme exists in both wheat and barley. It was active in an acid environment, was inhibited by zinc, but was not affected by EDTA. Two enzyme activities (31.0 and 32.0 kD) are unique to the common wheat. Contribution from Agriculture Research Division, University of Nebraska, Journal Series No. 9895.  相似文献   

18.
Rye (Secale cereale L.), wheat (Triticum aestivum L.), and annual ryegrass (Lolium multiflorum Lam.) are commonly double cropped with soybean (Glycine max L.). Recent greenhouse studies have shown variability in plant-parasitic nematode response to cool season grass species and cultivars. However, subsequent soybean performance was not affected by previous annual ryegrass cultivar in the green-house. The objective of this research was to determine whether winter cover crop species or cultivars affected nematode populations and subsequent performance of soybean in teh field. Four cultivars of annual ryegrass, wheat, and rye, and a fallow control were seeded on a Suffolk sandy loam (fine-loamy, siliceous, thermic Typic Hapuldult) soil in each of three years. Nematode-susceptible soybeans were seeded following forage removal. Soil samples for nematode counts were taken immediately before soybean harvest each year. In another experiment, one cultivar each of annual ryegrass, wheat, and rye, and a fallow control were followed by three soybean cultivars selected for differing nematode susceptibility. Grass cultivars did not affect nematode populations under succedding soybean. The only nematodes affected by grass species in either experiment were Pratylenchus spp., Heterodera glycines Ichinohe, and Tylenchorhynchus claytoni (Kofoid and White) Chitwood. Nematode population means were usually low following ryegrass and high following the fallow control. High soybean yields followed the fallow control, and low soybean yields followed annual ryegrass.  相似文献   

19.
Destruction of guard cell nuclei in epidermis isolated from leaves of pea, maize, sunflower, and haricot bean, as well as destruction of cell nuclei in leaves of the aquatic plants waterweed and eelgrass were induced by cyanide. Destruction of nuclei was strengthened by illumination, prevented by the antioxidant alpha-tocopherol and an electron acceptor N,N,N ,N -tetramethyl-p-phenylenediamine, and removed by quinacrine. Photosynthetic O2 evolution by the leaf slices of a C3 plant (pea), or a C4 plant (maize) was inhibited by CN- inactivating ribulose-1,5-bisphosphate carboxylase, and was renewed by subsequent addition of the electron acceptor p-benzoquinone.  相似文献   

20.
Phloem sap was collected from white lupin (Lupinus albus L.), cowpea (Vigna unguiculata L.) and castor bean (Ricinus communis L.) and analysed for gibberellins (GAs) using gas chromatography-mass spectrometry (GC-MS). A large number of GAs were found in the phloem exudate of all three species, particularly where the sap was collected from pods (white lupin and cowpea) and in these legumes GAs representing both the early C-13-hydroxylation and non-hydroxylation pathways of biosynthesis were identified. In the sap collected from the vegetative tissues of castor bean the number of GAs identified was fewer than that in the other species, representing mainly the non-hydroxylation pathway. Data from sap collected from the pedicel and stylar ends of pods and by making feeds of radiolabelled GAs to seeds in situ in white lupin indicate that the GAs present in the phloem are derived mainly from the vegetative tissues of the plant. No evidence for metabolism of GAs in the phloem could be found.  相似文献   

设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号