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1.
Otakar Rop Jiri Mlcek Tunde Jurikova Magdalena Valsikova 《Central European Journal of Biology》2012,7(4):672-679
At present, Cape gooseberry (Physalis peruviana) fruit is one of the less used raw materials of plant origin, which can be used for human nutrition. This fruit, as well
as alimentary products made of it, were used by healers in folk medicine in the distant past. The aim of this study was to
monitor and evaluate the antioxidant capacity of fresh fruit of three Cape gooseberry cultivars ‘Giant’, ‘Golden berry’ and
‘Inka’. Antioxidant capacity was also tested, on the basis of the scavenging effect of reactive oxygen species (ROS) and lipid
peroxidation of methanolic extracts made of fresh fruit. These results were further extended and supplemented with determinates
of the vitamin C and total phenolic contents. These analyses were made for three consecutive years. The highest values of
antioxidant capacity were observed in the ‘Inka’ cultivar (9.31 grams of ascorbic acid equivalents kg−1 of fresh mass). In this cultivar, the obtained results were corroborated also in ROS and the contents of vitamin C and total
phenolics. Due to a high antioxidant capacity of this fruit species, the results presented should increase its popularity
above all as a promising raw material, which can be used for human nutrition. 相似文献
2.
S. Doganlar S. D. Tanksley M. A. Mutschler 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,100(2):249-255
Using RAPD marker analysis, two quantitative trait loci (QTLs) associated with earliness due to reduced fruit-ripening time
(days from anthesis to ripening = DTR) were identified and mapped in an F2 population derived from a cross between Lycopersicon esculentum’E6203’ (normal ripening) and Lycopersicon esculentum’Early Cherry’ (early ripening). One QTL, on chromosome 5, was associated with a reduction in both ripening time (5 days)
and fruit weight (29.3%) and explained 15.8 and 13% of the total phenotypic variation for DTR and fruit weight, respectively.
The other QTL, on chromosome 12, was primarily associated with a reduction only in ripening time (7 days) and explained 12.3%
of the total phenotypic variation for DTR. The gene action at this QTL was found to be partially dominant (d/a=0.41). Together,
these two QTLs explained 25.1% of the total phenotypic variation for DTR. Additionally, two QTLs associated with fruit weight
were identified in the same F2 population and mapped to chromosomes 4 and 6, respectively. Together, these two QTLs explained 30.9% of the total phenotypc
variation for fruit weight. For all QTLs, the ’Early Cherry’ alleles caused reductions in both ripening time and fruit weight.
The polymorphic band for the most significant RAPD marker (OPAB-06), linked to the reduced ripening time QTL on chromosome
12, was converted to a cleaved amplified polymorphism (CAP) assay for marker-aided selection and further introgression of
early ripening time (DTR) into cultivated tomato.
Received: 15 March 1999 / Accepted: 29 April 1999 相似文献
3.
Effect of ethephon on sweet cherry pedicel-fruit retention force and quality is cultivar dependent 总被引:1,自引:0,他引:1
Ethephon (2-chloroethyl phosphonic acid) is effectively used to promote development of the pedicel-fruit abscission zone in
tart cherry (Prunus cerasus) for mechanical harvest. Our research program is investigating the use of ethephon to promote pedicel-fruit retention force
(PFRF) reduction on new sweet cherry (P. avium) cultivars to assist mechanized harvest and its affect upon fresh market quality fruit. Ethephon treatments were made at
different timings and rates to ‘Bing’ and ‘Chelan’ during the 2006 season. Ethephon applications to ‘Bing’ trees more than
10 days prior to harvest were effective at reducing PFRF and facilitating mechanical harvest, irrespective of rate (1.2, 3.5,
5.8 L ha−1 [1, 3, 5 pt A−1]). Ethephon applied fewer than 10 days prior to harvest did not reduce PFRF sufficiently. In contrast, no rate or timing
of ethephon studied induced a reduction in ‘Chelan’ PFRF sufficient for mechanical harvest. Accompanying PFRF analyses, fruit
quality was assessed by measuring firmness (g mm−1), soluble solids (oBrix), weight (g) and color (CTIFL, scale 1–7). Ethephon applied 22 days before harvest at a rate of 3.5 L ha−1 enhanced exocarp color in ‘Bing’ by 27%, while reducing firmness in both ‘Bing’ (−19%, 22 days prior to harvest) and ‘Chelan’
(−15%, 20 days prior to harvest). We observed a significant natural decline in ‘Skeena’ PFRF to levels acceptable for mechanical
harvest. This research documents genotypic-specific pedicel-fruit abscission characteristics and the potential to facilitate
mechanical harvest of fresh market quality sweet cherry fruit using ethephon. 相似文献
4.
5.
J. A. Buso L. S. Boiteux G. C. C. Tai S. J. Peloquin 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,101(1-2):139-145
Diploid potato clones with 2n-pollen formation by first-division restitution without crossing-over (FDR-NCO) are ideal testers
to estimate the breeding value of elite 4x cultivars by virtue of transmitting their genotypes practically intact to their progenies. This characteristic facilitates
genetic analysis, since meiotic recombination would take place only in the 4x parent and not in the diploid parent. We evaluated (under short-day conditions) families from complete factorial crosses
between four 4x cultivars and five 2x(FDR-NCO) clones. Families were compared with two standard 4x cultivars (’Bintje’ and ’Delta’) for total tuber yield (TTY), commercial yield (CY), haulm maturity (HM), plant vigor (PV),
plant-top uniformity (PU), eye depth (ED), number of tubers per hill (NTH), and the CY/TTY index (CTI). For TTY, the contrasts
family group (310 g/ hill) vs ’Delta’ (430 g/hill) and the family group vs ’Bintje’ (210 g/hill) were significant. Only 25%
of the families were different from ’Delta’ and 20% of them outyielded ’Bintje’. For CY, differences were observed between
families (240 g/hill) vs ’Delta’ (340 g/hill) and families vs ’Bintje’ (150 g/hill). The two best families had 53% CY over
’Bintje’. Surprisingly, only one family had a higher NTH than ’Bintje’. No differences were observed for HM. Seventy five
and 30% of the families had an ED similar to ’Delta’ (ED = 2) and ’Bintje’ (ED = 1), respectively. A multivariate analysis
indicated that 63% of the data variability could be explained by two factors. TTY, CY, and PV had high loading on the first
factor, whereas ED, PU and HM had high loading on the second factor; CTI and NTH had equal sizes on both factors. High TTY
and PV were associated with high NTH and CTI. Deep eye, PU, and late maturity were associated with high NTH and reduced CTI.
The distributions of factor scores of the entries indicated that some 2x parents had strong influences (irrespective of the direction of their effects) on the crosses. Six crosses due to two 2x males were in the ’Bintje’ quarter with negative scores for both factors (implying low TTY, poor vigor, and low NTH). Also
three crosses due to another 2x clone were distributed in the quarter of positive factor 1 and negative factor 2. These crosses plus another one were in
the same quarter of ’Delta’ (implying high yields, low ED, low PU, and early maturity). The FDR-NCO clones provide a homogeneous
sample of heterozygous 2n-gametes allowing the unique opportunity to estimate the relative contribution of the random meiotic
products (from the 4x parents) and the ’somatic’ 2x genome for the phenotypic expression of quantitative traits. The interesting result was that measurable effects (favorable
or not) on the data variability were mainly determined by the genomic contribution of the haploid-species hybrids. Three out
of five 2x-male parents showed rather strong effects on progenies. No such effects were observed on the four 4x-female parents.
Received: 3 September 1999 / Accepted: 24 November 1999 相似文献
6.
Antonín Dreiseitl 《Biologia》2011,66(5):762-767
Resistance to causal agents of diseases is an important varietal characteristic that influences the management practice of
crop plants and thus production costs of commodities. At present, almost all European barley varieties possess at least one
major gene for resistance to powdery mildew. After hybridizing selected parental varieties, resistance genes often segregate
in subsequent generations and, therefore, some varieties comprise lines that differ in the number or combinations of resistance
genes. The objective of this research was to describe the various methods available for postulating resistance genes to pathogens
in heterogeneous varieties using resistance to powdery mildew of barley as an example. Four spring barleys (‘Orbit’, ‘Malva’,
‘Tocada’ and CLE 233), and a six-row variety of winter barley, F 12872, were screened. For postulating resistance genes, several
testing procedures and many Blumeria graminis f.sp. hordei isolates were used. Minimum amounts of seed were determined and different methods of obtaining homogeneous seed samples from
heterogeneous varieties were compared. It was found that ‘Orbit’ and ‘Malva’ are composed of three and ‘Tocada’, CLE 233 and
F 12872 of two lines with different resistances to powdery mildew. Problems of postulating resistance genes in heterogeneous
varieties and the advantages of testing leaf segments instead of young plants are discussed. 相似文献
7.
Unopened leaves, petioles and fully opened leaves from micropropagation cultures of five Vitis rotundifolia Michx. varieties were cultured on induction medium to study their embryogenic response. Among the various explants tested,
the maximum number of varieties produced embryogenic cultures from unopened leaves followed by fully opened leaves and petioles.
Based on morphological differences, two types of embryogenic cultures were identified. Friable cultures typically arose as
proembryonic masses (PEM) on induction medium, whereas somatic embryo production without an intervening PEM stage was observed
in compact cultures. Of the five varieties tested, the highest frequency of embryogenic response was observed from fully opened
leaves of ‘Supreme’ and unopened leaves and petioles of ‘Delicious’. Attempts to initiate suspension cultures from varieties
resulted in proliferation and maintenance of ‘Alachua’ and ‘Carlos’ cultures in liquid medium for 16 weeks. Embryogenic potential
of varieties was studied on cultures growing on embryo development medium. The maximum number of cotyledonary stage somatic
embryos from 0.2 g proembryonic masses were observed in ‘Carlos’ (379.3) followed by ‘Alachua’ (350.0) and ‘Delicious’ (305.0).
Cotyledonary stage somatic embryos germinated when cultured on Murashige and Skoog medium containing 1 μM Benzyladenine (BA).
Although high embryo germination rates (80–100%) were observed in the varieties tested, plant recovery from germinated somatic
embryos ranged from 6–47%. Embryogenic cultures could be maintained on X6 medium and used in genetic engineering studies. 相似文献
8.
J. G. Roddick G. Melchers 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1985,70(6):655-660
Summary Analyses of leaves and ‘tubers’ from somatic hybrids of potato and tomato (‘pomato’ with plastids of potato, ‘topato’ with
plastids of tomato) produced by fusion of protoplasts from liquid cultures of dihaploid potato and mesophyll of tomato revealed
the presence of the two major potato glycoalkaloids (α-solanine and α-chaconine) as well as the tomato glycoalkaloid (αtomatine). The total alkaloid content of leaves was greater than that of ‘tubers’ and similar to levels in the foliage of
parent plants. However, glycoalkaloids were more abundant in hybrid ‘tubers’ than in normal potato tubers by a factor of 5–15.
In hybrid foliage, approximately 98% of the alkaloid present was of potato origin whereas in ‘tubers’ the reverse was the
case, with tomatine comprising 60–70% of the total alkaloid. The similarities in alkaloid content and ratios between the pomato
and the topato lines indicate that plastomes do not influence the biosynthesis and distribution of these alkaloids. The results
indicate that major secondary metabolites may prove useful for assessing the hybrid nature of such plants. 相似文献
9.
QTL mapping of aroma compounds analysed by headspace solid-phase microextraction gas chromatography in the apple progeny ‘Discovery’ × ‘Prima’ 总被引:2,自引:0,他引:2
F. Dunemann D. Ulrich A. Boudichevskaia C. Grafe W. E. Weber 《Molecular breeding : new strategies in plant improvement》2009,23(3):501-521
Improving fruit quality of apple varieties is an important but complex breeding goal. Flavour is among the key factors of
apple fruit quality but in spite of the analytical and biochemical knowledge about volatiles little is known about the genetic
and molecular bases of apple aroma. The aim of this study was to use a saturated molecular linkage map of apple to identify
QTLs for aroma compounds such as alcohols, esters and terpenes, but also for a number of unidentified volatile compounds (non-targeted
analysis approach). Two parental genetic maps were constructed for the apple cultivars ‘Discovery’ and ‘Prima’ by using mainly
AFLP and SSR markers. ‘Discovery’ and ‘Prima’ showed very different volatile patterns, and ‘Discovery’ mostly had the higher
volatile concentrations in comparison with the Vf-scab resistant ‘Prima’ which has its origin in the small-fruited apple species Malus floribunda. About 50 putative QTLs for a total of 27 different apple fruit volatiles were detected through interval mapping by using
genotypic data of 150 F1 individuals of the mapping population ‘C3’ together with phenotypic data obtained by head-space solid phase microextraction
gas chromatography. QTLs for volatile compounds putatively involved in apple aroma were found on 12 out of the 17 apple chromosomes,
but they were not evenly dispersed. QTLs were mainly clustered on linkage groups LG 2, 3 and 9. In a first attempt, a LOX
(lipoxygenase) candidate gene, putatively involved in volatile metabolism, was mapped on LG 9, genetically associated with
a cluster of QTLs for ester-type volatiles. Implications for aroma breeding in apple are discussed. 相似文献
10.
Patrick H. Brown Steven A. Weinbaum Geno A. Picchioni 《Trees - Structure and Function》1995,9(3):158-164
The influence of alternate bearing on nutrient utilization and total tree nutrient content was investigated in mature pistachio
(Pistacia vera L. cv Kerman trees). Removal of N, P and Zn in fruit and abscised leaves of cropping (‘on’) trees averaged 5, 6, and 2 times,
respectively, the removal in abscised leaflets of the non-fruiting, ‘off’ year trees. One hundred and thirty-five kg N, 131
kg K, 86 kg Ca, 39 kg Mg and 18 kg P per hectare were removed in fruits and abscised leaves in ‘on’ year trees. Tree nutrient
contents and, presumably, the size of nutrient storage pools in dormant trees varied between ‘on’ and ‘off’ years. There was
22% and 14% more N and P, respectively, in dormant trees following ‘off’ than ‘on’ years. The greater N and P accumulation
in ‘off’ year trees is depleted in support of the large fruit demand for N and P during ‘on’ years. In contrast to N and P,
there was greater K and Ca accumulation in perennial tree parts during ‘on’ years than during ‘off’ years. The greater K accumulation
in perennial tree parts and approximately 30% greater removal of K in annual organs during ‘on’ than ‘off’ years suggests
that K uptake could be 4 times higher in ‘on’ year trees than in (non-cropping), ‘off’ year trees. 相似文献
11.
Localization of the rice stripe disease resistance gene, Stv-bi, by graphical genotyping and linkage analyses with molecular markers 总被引:4,自引:0,他引:4
Y. Hayano-Saito T. Tsuji K. Fujii K. Saito M. Iwasaki A. Saito 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,96(8):1044-1049
We used graphical genotyping and linkage analyses with molecular markers to determine the chromosomal location of the rice
stripe disease resistance gene, Stv-b
i
. The stripe resistance gene from the indica rice (Oryza sativa) cv ‘Modan’ was introgressed into several Japanese rice varieties. We found 4 RFLP markers in ‘Modan’, five susceptible parental
rice varieties (‘Norin No. 8’, ‘Sachihikari’, ‘Kanto No. 98’, ‘Hokuriku No.103’ and ‘Koganebare’) and four resistant progeny
varieties (‘St. No. 1’, ‘Aichi No. 6’, ‘Aoisora’ and ‘Asanohikari’). Graphical genotyping of the resistant progeny revealed
a chromosomal segment ascribable to ‘Modan’ and associated with stripe resistance. The chromosomal segment from ‘Modan’ was
located at 35.85 cM on chromosome 11. Linkage analysis using 120 F2 individuals from a cross between ‘Koshihikari’ (susceptible) and ‘Asanohikari’ (resistant) revealed another 8 RFLP markers
in the same chromosome. We performed a bioassay for rice stripe resistance in F3 lines of the F2 individuals using infective small brown planthoppers and identified an 1.8-cM segment harboring the rice stripe disease resistance
gene, Stv-b
i
, between XNpb220 and XNpb257/ XNpb254. Furthermore, Stv-b
i
was linked by 0.0 cM to a RFLP marker, ST10, which was developed on the basis of the results of RAPD analysis. These DNA
markers near the Stv-b
i
locus may be useful in marker-assisted selection and map-based cloning of the Stv-b
i
gene.
Received: 26 September 1997 / Accepted: 4 November 1997 相似文献
12.
Summary A field experiment was designed to determine the salt tolerance of three varieties of sweet pepper for paprika, studying the
effects of four increasing levels of sodium chloride in the irrigation water (5, 10, 29 and 42 meq/l) on soil salinity, yields,
and quality of the fruits.
EC5:1 and the soluble sodium and chloride contents increased with increasing salinity. The yields were reduced by 5 per cent for
variety ‘Ramillete’, 19 per cent for ‘Tres cascos’, and 40 per cent for ‘Bola’ at the highest level. The fruit quality variables
studied (colour index and chloride contents) differed with the variety. re]19750925 相似文献
13.
Shimpei Uraguchi Masako Kiyono Takuya Sakamoto Izumi Watanabe Katsuji Kuno 《Planta》2009,230(2):267-276
The contributions of cadmium (Cd) accumulation in cell walls, antioxidative enzymes and induction of phytochelatins (PCs)
to Cd tolerance were investigated in two distinctive genotypes of black oat (Avena strigosa Schreb.). One cultivar of black oat ‘New oat’ accumulated Cd in the leaves at the highest concentration compared to another
black oat cultivar ‘Soil saver’ and other major graminaceous crops. The shoot:root Cd ratio also demonstrated that ‘New oat’
was the high Cd-accumulating cultivar, whereas ‘Soil saver’ was the low Cd-accumulating cultivar. Varied levels of Cd exposure
demonstrated the strong Cd tolerance of ‘New oat’. By contrast, low Cd-accumulating cultivar ‘Soil saver’ suffered Cd toxicity
such as growth defects and increased lipid peroxidation, even though it accumulated less Cd in shoots than ‘New oat’. Higher
activities of ascorbate peroxidase (EC 1.11.1.11) and superoxide dismutase (EC 1. 15. 1. 1) were observed in the leaves of
‘New oat’ than in ‘Soil saver’. No advantage of ‘New oat’ in PCs induction was observed in comparison to Cd-sensitive cultivar
‘Soil saver’, although Cd exposure increased the concentration of total PCs in both cultivars. Higher and increased Cd accumulation
in cell wall fraction was observed in shoots of ‘New oat’. On the other hand, in ‘Soil saver’, apoplasmic Cd accumulation
showed saturation under higher Cd exposure. Overall, the present results suggest that cell wall Cd accumulation and antioxidative
activities function in the tolerance against Cd stress possibly in combination with vacuolar Cd compartmentation. 相似文献
14.
Zhentu Ying Thomas L. Davenport Taifang Zhang Raymond J. Schnell Cecile L. Tondo 《Plant Molecular Biology Reporter》2009,27(3):374-380
‘Hass’ is the most popular avocado (Persea americana Mill.) cultivar in the world. It has been characterized as a crop requiring cross-pollination. However, the potential extent
of self-pollination and the most effective pollen donors (best cross-pollinizing cultivars) have not been determined. In this
study, 56 markers were screened against ‘Hass’ and nine commonly used pollinizing cultivars grown in southern California:
‘Bacon,’ ‘Ettinger,’ ‘Fuerte,’ ‘Harvest,’ ‘Lamb Hass,’ ‘Marvel,’ ‘Nobel,’ ‘Sir Prize,’ and ‘Zutano.’ Seventeen microsatellite,
i.e., simple sequence repeat (SSR) markers, were found to be very promising for paternity analysis. Four highly informative
SSR markers were selected to accurately and unequivocally identify pollen parents of ‘Hass’ fruit from an orchard interplanted
with these pollinizing cultivars. From 2003 to 2006, 7,984 ‘Hass’ fruit were analyzed for their paternity. Overall, the pollen
parents of 99.55% of the analyzed fruit could be unequivocally identified with a single multiplex polymerase chain reaction
(PCR). Only 36 fruits (<0.45%) required a second PCR reaction to reach unequivocal identification of the pollen parents. 相似文献
15.
Ewa Hanus-Fajerska Maria Lech Anna Pindel Kazimierz Miczyński 《Acta Physiologiae Plantarum》2000,22(3):317-324
Protocols elaborated with the objective of achieving valuable material for selection procedure of variants with virusresistance
traits in tomato genotypes are presented. Preliminary results are demonstrated in the domain of testing for variability in
somaclones obtained through indirect adventitous organogenesis initiated on leaf explants of cultivated tomato (Lycopersicon esculentum Mill.). Somaclones were grown in greenhouse conditions and variation of their symptoms upon infection with tomato mosaic
(ToMV) or cucumber mosaic (CMV) respectively was observed. Tests for resistance to the local isolates of the above cited viruses
were performed using enzyme linked immunosorbent assay and back inoculation onto diagnostic plants. Screening data are presented.
Desirable variants were selected from cultivars ‘Moneymaker’, ‘Potentat’ and ‘Rutgers’. Some of the ‘Moneymaker’ somaclones
exhibited increased tolerance to cucumber mosaic virus, a few seemed to be even fully resistant though most were susceptible
as donor plants. The most favourable somaclonal lines are actually further tested and monitored for changes in horticultural
characteristics. The described procedure of searching for resistance trait in specific pathogen-free (SPF) plants regenerated
from infected tissue looks promising and thus can serve as aid in attaining appropriate objectives of breeding programme.
Additionaly experiments were initiated to obtain somaclones from cultivars ‘Beta’, ‘Krakus’ and Stevens Rodade hybrid via
regeneration of isolated protoplasts. To this end the callus stage was obtained from all donors. 相似文献
16.
Shoot tips and single node explants of two rootstocks (R.99 and 3309) and two varieties (‘Helwani’ and ‘Cabernet Franc’) of
grapevine cultured on DSD1 media for a period of 60 days, were irradiated with 0, 2, 5 and 7 Gy doses of gamma irradiation.
Shoot length of ‘Helwani’ and ‘Cabernet Franc’ was increased by 7 Gy irradiation. The 5 Gy dose increased the number of roots
in plants of the two rootstocks and ‘Helwani’. Root length of ‘Helwani’ and ‘Cabernet Franc’ at the 2 and 7 Gy doses were
significantly higher than those of the non-irradiated control. A similar effect was noticed on R.99 rootstock subjected to
5 Gy. Five Gy also increased the dry weight of the R.99 rootstock, whereas 2 and 7 Gy had a similar effect on ‘Helwani’ and
‘Cabernet Franc’. Number of leaves of plants exposed to 5 and 7 Gy was increased when compared with the non-irradiated control.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
17.
K. Sorkheh B. Shiran M. Khodambshi V. Rouhi S. Ercisli 《Plant Cell, Tissue and Organ Culture》2011,105(3):395-404
Eight native Iranian almond species from three sections, ‘Euamygdalus’ (Prunus communis; Prunus eleagnifolia and Prunus orientalis); ‘Lycioides’ (Prunus lycioides and Prunus reuteri) and ‘Spartioides’ (Prunus arabica, Prunus glauca and Prunus scoparia) were in vitro screened for drought tolerance using sorbitol and polyethylene glycol (PEG) as an osmoticum. Different levels
of water stress were induced using five concentrations of either sorbitol or polyethylene glycol in Woody Plant Medium (WPM).
Water potential of various media ranged from −0.80 to −2.05 MPa and water stress in culture medium adversely affected plantlet
growth. Wild species from ‘Spartioides’ were less affected than ‘Lycioides’ and ‘Euamygdalus’. At the same level of water
potential, sorbitol had lower adverse effects than PEG; the latter being severe. Prunus × sorbitol and Prunus × PEG interactions were significant. At 0.2 M sorbitol and 0.003 M PEG, ‘Spartioides’ produced significantly more roots with
higher total root length and root volume, as well as root-dry weight than those of ‘Lycioides’ and ‘Euamygdalus.’ It is concluded
that in vitro screening of native Iranian almond species under specific and limited water-stress conditions may provide a
system for effectively differentiating the wild species of almond for their expected root mass production under field conditions. 相似文献
18.
19.
20.
A parentage study of closely related Ukrainian wine grape varieties using microsatellite markers 总被引:1,自引:0,他引:1
S. Goryslavets V. Risovanna R. Bacilieri J. -F. Hausman M. Heuertz 《Cytology and Genetics》2010,44(2):95-102
Four bred grapevine varieties released for commercial cultivation in Ukraine, namely ‘Antey Magarachskii’, ‘Rubinovyi Magaracha’,
‘Granatovyi Magaracha’ and ‘Rubin Golodrigi’, and their putative parental forms were genotyped using six microsatellite loci.
Genotypes were compared with breeding records to verify genetic relationships among varieties. Results of the analysis confirmed
four of six parent-offspring relationships. Results of the analysis allow to assume that genotype ‘Seyve Villard 20347’ is
the direct parent of ‘Antey Magarachskii’ instead of its grandparent. The first-studied accession believed to be that of ‘Granatovyi
Magaracha’ was identified as impurity. In order to verify the parentage of ‘Granatovyi Magaracha’, rest accessions of that
variety and its putative parent ‘Antey Magarachskii’ were additionally genotyped at 13 nuclear loci and at three chloroplast
loci. The parent-offspring relationship was confirmed, as all ‘Granatovyi Magaracha’ accessions had a common allele with the
parent variety ‘Antey Magarachskii’ at each locus and the same chlorotype A. Different ‘Granatovyi Magaracha’ accessions could
have been obtained via vegetative propagation of two seedlings which arose from one crossing. 相似文献