共查询到18条相似文献,搜索用时 281 毫秒
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低强度激光的生物效应对组织修复的影响 总被引:3,自引:0,他引:3
本文概述了近几年低强度激光照射疗法对促进骨骼肌的再生、关节炎与骨折的修复、改善心肌微循环等方面生物效应的实验研究的新进展,其结果表明低强度激光照射组织具有显著的生物刺激作用和损伤后的修复功能。 相似文献
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关于低强度激光疗法的机理及其临床应用研究 总被引:2,自引:0,他引:2
刘承宜等人提出了低强度激光疗法的生物信息模型。根据BIML,绿,兰和紫等冷色兴奋Ga蛋白介导的生理过程,红,产黄等暖色兴奋Gq或Gi蛋白或受体关联蛋白酶介导的生理过程。本文将激光的生物效应分为直接效应和间接效应两类,根据BIML成功地解释了已知第二信使生理功能的系统的低强度激光照射的作用机理和临床应用,可望用于开发低强度激光疗法的临床新应用。 相似文献
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低强度激光鼻腔照射对血脂异常作用的研究 总被引:3,自引:0,他引:3
目的:观察低强度激光(650nm/5mw)鼻腔照射疗法对血脂异常的作用。方法:30例原发性血脂异常患者随机分为两组,分别接受药物(舒降之)治疗和低强度激光鼻腔照射疗法,测定治疗前后的血脂水平。结果:在调整饮食的基础上,低强度激光经鼻腔照射使病人的血清总胆固醇水平降低了5.8%(P=0.031),甘油三酯降低了8.8%;低强度激光鼻腔照射在有效调整血脂的同时,使谷丙转氨酶(J唧)水平下降了23.1%(P=0.022),对肝功能具有保护作用。结论:与舒降之相比,低强度激光鼻腔照射疗法的调脂效果虽然相对较弱,但基于安全性的考虑,在饮食控制无效或肝功能异常的病人中使用低强度激光鼻腔照射仍然具有一定的应用价值。 相似文献
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He—Ne激光照射酵母菌的生物学效应 总被引:1,自引:0,他引:1
激光照射酵母菌可产生一系列生物学效应。可刺激酵母的生长、繁殖,降低细胞内核酸含量。其效应强度与照射的激光剂量(光功率密度)密切相关。而且,酵母对低剂量连续多次的激光照射有一定的适应性。 相似文献
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低强度532nm与633nm激光血管内照射生物效应比较 总被引:6,自引:1,他引:5
目的:研究同等照射条件的低强度532nm与633nm激光血管内照射对家兔白细胞计数与淋巴细胞凋亡的影响,比较两种激光生物效应的特点。方法:用532nm和633nm激光对健康日本大耳白家兔血管内照射,平均照射功率均设在5mW左右,照射总能量约12J。两组家兔均于照前及照后1d、4d、7d、11d进行外周血白细胞计数,于照前及照后1d、5d进行淋巴细胞凋亡分析。结果:532nm激光照射后,家兔外周血白细胞计数表现为先显著升高后趋向恢复,633nm激光照射后白细胞计数变化类似,但与照前相比升高不明显;与照前相比,两组家免外周血淋巴细胞凋亡比例于照后1d均明显降低,照后5d均显著升高;两组家兔相比,照射后白细胞计数差别明显,但淋巴细胞凋亡比例差异不显著。结论:同等照射条件下,低强度532nm与633nm激光照射血液的生物效应相似,都可以促进白细胞的代谢更新,只是532nm激光的效应略强一些。 相似文献
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低强度He-Ne激光对红细胞变形性的影响已得到广泛的认可和应用,但其具体调节机制尚不明确,通过研究低强度He-Ne激光对红细胞胞浆内钙离子浓度的影响,探讨其对红细胞变形性影响的机制。采用A23187处理过的红细胞,分别用5 mW和9 mW激光照射后,观察红细胞胞浆内钙离子浓度变化。结果显示:低强度He-Ne激光照射后的红细胞与无照射组的红细胞相比,红细胞胞浆内钙离子浓度显著降低,但在本实验中钙离子浓度的降低与激光照射剂量无显著相关。由此得出结论:降低红细胞胞浆内钙离子浓度可能是低强度He-Ne激光调节红细胞变形性的重要机制。 相似文献
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穴位激光照射的剂量和机理初探 总被引:4,自引:0,他引:4
穴位激光照射疗法是一种通过低强度激光束直接照射穴位的治疗方法,具有针灸作用。阐述了激光照射机体穴位在临床应用的本质,并对其使用的光剂量和作用机理做了初步讨论和分析。 相似文献
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目的:研究He-Ne激光照射鼠巨噬细胞对线粒体跨膜电势的影响,及其与激光剂量的关系。方法:用亲脂性阳离子荧光染料Rhodamine123对鼠巨噬细胞线粒体作荧光标记,以不同的激光剂量照射,采用图像分析系统(IAS)和荧光显微镜观察线粒体跨膜电势荧光强度的变化。结果:低功率He-Ne激光照射5,10,15min,激光剂量分别为0.649,1.388和2.082J/cm^2,巨噬细胞线粒体跨膜电势荧光 相似文献
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M. S. Romaniuk M. V. Bura S. M. Mandzynets O. R. Kulachkovsky D. I. Sanagursky 《Cytology and Genetics》2014,48(3):171-174
The ultrastructural organization of loach embryo cells (Misgurnus fossilis L.) at the stage of the first and the tenth embryo divisions was investigated under the influence of low intensity helium-neon laser irradiation of 5 min exposure. It was determined that the effect of laser irradiation led to ultrastructural changes: the shape of mitochondria and multivesicular bodies changes. The obtained results explain the possible mechanism of influence of low-intensity laser irradiation at the cellular level. 相似文献
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低能量激光与集落刺激因子对人脐带血造血细胞的增殖作用 总被引:1,自引:0,他引:1
本研究用铜蒸气激光照射人脐带带血造血细胞,观察低能量激光与集落因子对造血细胞的增殖作用。结果显示激光加CSF对造血细胞GM-CFUc有协同增殖作用,与单用激光照射组,CSF刺激组及对照组相比,有非常显著性差异。 相似文献
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在临床应用中,低功率He-Ne激光(632.8 nm)能促进骨骼肌修复,加速创伤愈合,降低牙齿的超敏感性,减缓疼痛等.大量研究表明:低功率He-Ne激光能调节细胞的众多行为,如细胞增殖、分泌、迁移、粘附、蛋白质合成和基因表达等.但低功率He-Ne激光调节细胞行为的分子机制并未阐明,考察低功率He-Ne激光照射后细胞内活性氧自由基的产生水平和游离ca2 浓度是否会发生变化,通过激光扫描共聚焦显微镜,分别利用H:DCFDA和Fluo-3/AM这两种荧光探针,检测到经He-Ne激光照射后,肺腺癌细胞内活性氧自由基的水平上调以及游离Ca2 浓度增加.该研究为低功率He-Ne激光的生物光刺激效应提供了可能的分子机理. 相似文献
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The role of lipid peroxidation products formed in membranes of human blood leukocytes after irradiation with He-Ne laser was studied. It was found that low-intensity laser irradiation (0.3-1.6 J/cm2) leads to both cell activation and an increase in the content of lipid peroxidation products. The intensity of lipid peroxidation was analyzed by estimating the amount of TBA reactive products and lipid diene conjugates. Irradiation in the presence of an exogenous photosensitizer (protoporphyrin IX) enhanced the phenomena observed. The use of antioxidants (tocopherol and ionol) completely eliminated the laser-induced effects (changes in leukocyte activity and accumulation of lipid peroxidation products). These results can be explained by the fact that laser irradiation leads to the activation of lipid peroxidation in leukocyte membranes, which in turn enhances the response of cells to the stimulus (priming). 相似文献
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Generation of ROS in cells on exposure to CW and pulsed near-infrared laser tweezers. 总被引:1,自引:0,他引:1
Samarendra Kumar Mohanty Mrinalini Sharma Pradeep Kumar Gupta 《Photochemical & photobiological sciences》2006,5(1):134-139
We report the results of a study on generation of reactive oxygen species (ROS) and changes in the membrane potential of mitochondria of carcinoma of cervix (HeLa) and Chinese hamster ovary (CHO) cells following exposure to continuous wave (cw) or pulsed Nd: YAG laser (1064 nm). For a given laser irradiation, the generation of ROS and induced changes in the membrane potential of mitochondria were more pronounced for HeLa cells as compared to CHO cells. However, in both the cells the laser dose required to elicit a given change was much lower with pulsed laser exposure compared to that required with a cw laser exposure. This suggests involvement of photothermal effects in the laser irradiation induced changes. Mechanistic studies using quenchers for ROS suggest that laser irradiation leads to generation of hydroxyl radicals. 相似文献
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The He-Ne-laser induced effects in human blood leukocytes in the presence of autologic plasma were investigated. Experiments were performed in two ways: (1) He-Ne-laser irradiation of cells in the presence of autologic plasma or (2) laser irradiation and subsequent addition of autologic plasma to the cell suspension. The concentration dependencies of plasma additions were evaluated. To obtain different concentrations of porphyrins in plasma samples, we either diluted the samples with PBS or selected patients with different porphyrin plasma content. The effects of He-Ne-laser irradiation were characterized by the maximum effect dose (Dmax) of irradiation and the degree of maximum cell activation (Amax, priming index). In the first series of experiments, we irradiated leukocytes in the presence of autologic plasma taken from patients with pneumonia and bronchial asthma. It was found that Dmax decreased with increasing porphyrin concentration in plasma. It was observed that, at low porphyrin concentrations, Amax increased severalfold with increasing photosensitizer concentration. At a porphyrin concentration of 0.46 pmol a decrease in Amax was detected as the porhyrin concentration increased. The same effects were revealed at high doses of laser irradiation. Very similar effects were found in experiments with the addition of irradiated plasma to cells. However, the Amax value was considerably less compared to that after irradiation in the presence of plasma (160% vs. 230 - 270% upon combined irradiation of cells and plasma). The Dmax value was higher in the series of experiments in which plasma was irradiated separately. The results suggest that laser-induced leukocyte activation can be mediated by blood plasma porphyrins and the products of lipid peroxidation formed as a result of porphyrin-photosensitized lipid oxidation. 相似文献