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1.
In Vitro Antibacterial Activity of Minocycline and Effect of Agar Medium Utilized in Its Susceptibility Testing 总被引:4,自引:0,他引:4 下载免费PDF全文
The in vitro activity of minocycline against 1,028 bacterial strains was determined in parallel in Mueller Hinton Agar and Trypticase Soy Agar. The broad antibacterial effect of minocycline against gram-positive cocci and gram-negative bacilli is confirmed. Minimal inhibitory concentrations for gram-positive bacteria in Mueller Hinton Agar were at least twofold less than in Trypticase Soy Agar. Minimal inhibitory concentrations for gram-negative bacilli in Mueller Hinton Agar were usually fourfold less than in Trypticase Soy Agar. 相似文献
2.
Monika Łysakowska Andrzej Denys Monika Sienkiewicz 《Central European Journal of Biology》2011,6(3):405-413
The aim of this work was to investigate the antimicrobial properties of thyme essential oil against clinical multiresistant
strains of Acinetobacter spp. The antibacterial activity of oil was tested against standard and clinical bacterial strains of Acinetobacter genus. The agar diffusion method was used to check the inhibition of microbial growth at various concentrations of the oil
from Thymus vulgaris. Susceptibility testing to antibiotics and chemotherapeutics was prepared using the disc-diffusion method. Identification
of bacterial strains was carried out with the Vitek system and confirmed by PCR for Acinetobacter baumanii gyrB gene. The results of experiments showed that the oil from T. vulgaris exhibited an extremely strong activity against all of the clinical strains of Acinetobacter. Thyme oil demonstrated a very good efficacy against multiresistant strains of tested bacteria. Essential oils seems to be
an excellent alternative for synthetic preparations and that is reason for an extensive assessment of their antimicrobial
activity.
相似文献
3.
During tissue culture of black locust (Robinia pseudoacacia L.), serious problems with plant-associated bacteria led to a reduction of propagation potential in several clones. Four
dominant strains of plant-associated bacteria could be isolated and were assigned to the genera Acidovorax, Dyella, Microbacterium and Sphingomonas. Out of five essential oils tested, thyme and lemongrass oil at a concentration of 0.03% each and 0.015% of both oils in
combination clearly inhibited the growth of these bacteria strains on bacteriologic medium. There were no significant differences
in total bacterial population density when penicillin, thyme and lemongrass oil or thyme plus lemongrass oil were added to
the plant propagation media. The use of lemongrass oil changed the proportion of dominant bacterial strains. 相似文献
4.
Mi-Jin Park Won-Sil Choi Ha-Young Kang Ki-Seob Gwak Geun-Shik Lee Eui-Bae Jeung In-Gyu Choi 《Journal of microbiology (Seoul, Korea)》2010,48(4):496-501
In this study, the antibacterial activity of essential oil from Chamaecyparis obtusa (Sieb. et Zucc) leaves and twigs was investigated. The test strains were Klebsiella pneumoniae, Listeria monocytogenes, Salmonella typhimurium, Staphylococcus aureus, Escherichia coli, Legionella
pneumophila, and Methicilline-resistant Staphylococcus aureus. Antibacterial activity was estimated by measuring bacterial growth inhibition. Histopathological examination was also performed.
C. obtusa oil distinctly inhibited the growth of all test strains and exhibited the strongest antibacterial activity against L. monocytogenes. It was chromatographically divided into several fractions. The fractions were further tested against antibacterial activity
and their chemical compositions were analyzed. The fraction containing terpinen-4-ol (TA) showed high antibacterial activity
toward all strains tested. Tests with authentic samples showed that TA played a major role in the antibacterial activity of
C. obtusa oil, and in a mice test, the oil actively minimized inflammation by S. aureus. 相似文献
5.
P. Angelini R. Pagiotti B. Granetti 《World journal of microbiology & biotechnology》2008,24(2):197-202
Melaleuca alternifolia (tea tree) essential oil was investigated for its “in vitro” ability to control Trichoderma harzianum, a fungal contaminant that causes extensive losses in the cultivation of Pleurotus species. The antifungal activity of M. alternifolia essential oil and antagonist activities between Pleurotus species against three T. harzianum strains were studied in dual-culture experiments on an agar-based medium in which different concentrations of essential oil
were incorporated. M. alternifolia essential oil at a concentration of 0.625 μL/mL, inhibited T. harzianum mycelial growth by 5.9–9.0%, depending on the strain. At the same concentrations P. ferulae and P. nebrodensis stimulated mycelial growth by 5.2–8.1%. All strains of T. harzianum were antagonistic to the Pleurotus species in the control. When essential oil was added to the substrate cultural, the antagonistic activity of T. harzianum against the Pleurotus species was weak (0.0625 μL of essential oil) or non-existent (0.125 μL of essential oil). M. alternifolia essential oil could be an alternative to the synthetic chemicals that are currently used to prevent and control T. harzianum in mushroom cultivation. 相似文献
6.
The in-vitro biological activity of N-benzylsalicylthioamides against 8 bacterial strains was determined by broth microdilution method; results were compared with
those obtained with neomycin, penicillin G, ciprofloxacin and penicillin V. The compounds showed moderate to high activity
against G+ bacteria; especially compounds 4, 6, 13, 16–21 and 24 exhibited comparable or higher activity than reference drugs. The antibacterial activity was analyzed by quantitative structure-activity
relationship (QSAR). The antibacterial activity increased with lipophilicity, with the presence of halogens and with increasing
value of Hammet substituent constant σ. 相似文献
7.
Summary The antibacterial efficacy of the methanol extract of the aerial parts of Seseli libanotis by using disc diffusion assay against 107 strains from 52 bacterial species and the chemical composition of the essential
oil using GC/MS analysis were studied for the first time. The minimum inhibitory concentrations (MIC) of single compounds
were determined by the microbroth dilution method. Gas chromatography–mass spectrophotometry analyses allowed 13 compounds
to be determined; the main constituents of the essential oil of aerial parts of Seseli libanotis were trans-caryophyllene (20.39%), spathulenol (11.89%), (−)-caryophyllene oxide (11.47%), euasarone (10.66%) and delta-cadinene (9.16%).
The methanol extract of Seseli libanotis had a broad-spectrum antibacterial activity (>14 mm inhibition zone in diameter) in particular against Bacillus cereus, Bacillus dipsauri, Bacillus lentimorbus, Bacillus sphaericus, Bacillus subtilis, Corynebacterium ammoniagenes,
Kocuria rosea, Neisseria subflava and Micrococcus lylae. These inhibitory effects are interesting in relation to the prevention of microbial contamination in foods. 相似文献
8.
Mejdi Snoussi Hafedh Hajlaoui Emira Noumi Donatella Usai Leonardo Antonio Sechi Stefania Zanetti Amina Bakhrouf 《World journal of microbiology & biotechnology》2008,24(12):3071-3076
The chemical composition of five aromatic plants (Mentha longifolia, M. pulegium, Eugenia caryophyllata, Thymus vulgaris and Rosmarinus officinalis) frequently used in food preparation in Tunisia was analysed by GC-MS. The antimicrobial effect of the essential oils obtained
from these plants was tested against Vibrio alginolyticus, Vibrio parahaemolyticus, Vibrio vulnificus and Vibrio fluvialis strains. Thyme oil exhibited a high level of antimicrobial activities against Vibrio spp. strains. The diameter of the zones of growth inhibition for V. parahaemolyticus species was interestingly high (ranging from 14.66 to 28 mm). The MIC and MBC values were interestingly low for thyme oil
(MIC 0.078–0.156 mg/ml) and (MBC >0.31–1.25 mg/ml). These results showed that these plants especially thyme and clove, can
be to be used for seafood preparation to protect against contamination by Vibrio spp. strains.
An erratum to this article can be found at 相似文献
9.
Syed Sultan Beevi Lakshmi Narasu Mangamoori Naveen Anabrolu 《World journal of microbiology & biotechnology》2009,25(3):465-473
Aqueous, methanol, ethyl acetate, and chloroform extracts of the root, stem, and leaf of Raphanus sativus were studied for antibacterial activity against food-borne and resistant pathogens. All extracts except the aqueous extracts
had significant broad-spectrum inhibitory activity. The ethyl acetate extract of the root had the potent antibacterial activity,
with a minimum inhibitory concentration (MIC) of 0.016–0.064 mg/ml and a minimum bactericidal concentration (MBC) of 0.016–0.512 mg/ml
against health-damaging bacteria. This was followed by the ethyl acetate extracts of the leaf and stem with MICs of 0.064–0.256
and 0.128–0.256 mg/ml, respectively and MBCs of 0.128–2.05 and 0.256–2.05 mg/ml, respectively. The ethyl acetate extracts
of the different parts of R. sativus retained their antibacterial activity after heat treatment at 100°C for 30 min, and their antibacterial activity was enhanced
when pH was maintained in the acidic range. Hence this study, for the first time, demonstrated that the root, stem, and leaf
of R. sativus had significant bactericidal effects against human pathogenic bacteria, justifying their traditional use as anti-infective
agents in herbal medicines. 相似文献
10.
Many microbes associated with marine organisms have antimicrobial activity. We report the isolation of bacteria associated
with Amphipholis gracillima that have broad-spectrum antibacterial activity against a number of common bacterial strains. Fifty-eight isolates of bacilli
obtained from A. gracillima arm homogenates, from excised wound tissue, or from swabs of arm stumps exhibited 20–100% inhibition of one or more of 16
test bacteria at 35% salinity. Forty-one of the isolates were capable of 20–100% inhibition of one or more of 19 subject bacteria
at 10% salinity at 37°C. Three isolates, BE37, BE52, and BE53, exhibited the greatest range of antibacterial activity at both
10% and 35% salinity. Our results suggest that some of the bacteria associated with A. gracillima may provide the animal with chemical defenses against adverse bacterial infection. The water-soluble inhibitory chemicals
produced by the bacteria could potentially function as antimicrobial compounds against human pathogenic bacteria.
Received: 30 July 2001 / Accepted: 15 October 2001 相似文献
11.
Manoucher Shahamat Morris Levin Ishrat Rahman Christopher Grim John Heidelberg Gerard Stelma Rita Colwell 《Aerobiologia》1997,13(4):219-226
Disease transmission by airborne bacteria is well known. Bacterial burden in indoor air is estimated by sampling the air and
estimating Colony Forming Units (CFU) using a variety of media. In this study, the recovery of bacteria, after aerosolization
in an aerosol chamber, and employing a variety of media, was compared to that achieved using Tryptic Soy Agar medium. The
total number of cells present was determined by direct microscopy. All trials were conducted at approximately the same relative
humidity (RH) and temperature using the same collection device. Twelve species of bacteria were tested and a total of 120
media or media combinations were evaluated. Recovery on 64 media formulations was significantly lower for all strains examined,
and therefore, excluded from further consideration for the purposes of this study. Data for 56 of the media are presented.
Three species (Bacillus subtilis, Staphylococcus aureus andSerratia marcescens) were selected as representative for reporting and testing recovery success. It is concluded that, for the media included
in the study, there are large differences in recovery and successful recovery is related both to the effect of aerosolization
and the type of medium employed for recovery. Brain Heart Infusion Agar (with horse serum), Tryptic Soy Agar and Mueller Hinton
Agar yielded the best recoveries of aerosolized cultures. The most important finding was that only a small fraction of the
airborne bacterial populations, enumerated by direct microscopy, could be recovered on any of the media tested, suggesting
that culturable bacterial count is not a satisfactory means of estimating air microbial pollution. 相似文献
12.
Zoran Maksimović Marina Milenković Dragana Vučićević Mihailo Ristić 《Central European Journal of Biology》2008,3(2):149-154
This paper presents the results of a study on chemical composition and antimicrobial activity of Thymus pannonicus All. (Lamiaceae) essential oil from Vojvodina province (north of Serbia). The investigated oil was hydrodistilled from a flowering plant
and analysed by GC and GC-MS. Fifty-three constituents were identified (>97% of total oil), with geranial (41.42%, w/w) and
neral (29.61%, w/w) as the most prominent. The antimicrobial activity of the oil was evaluated using agar disc diffusion and
broth microdilution method against Staphylococcus aureus, Enterococcus faecalis, Pseudomonas aeruginosa, Escherichia coli, two strains of Klebsiella pneumoniae and two strains of Candida albicans. The essential oil exhibited antimicrobial activity to varying degrees against all tested strains. The maximum activity of
T. Pannonicus oil was observed against E. coli, S. aureus and both tested strains of C. Albicans (MIC = 50 μ/ml, each). Moderate activity was observed against P. aeruginosa and one of the tested strains of K. Pneumoniae (MIC = 200 μ/ml), while E. faecalis and the other strain of K. Pneumoniae expressed a higher degree of resistance (MIC > 200 μ/ml). This study confirms that essential oil of T. pannonicus possesses remarkable in vitro antimicrobial activity against several medicinally important pathogens. This is attributable to lemon-scented citral, a mixture
of geranial and neral, which has well-documented antimicrobial activity against a range of bacteria and fungi. 相似文献
13.
Hari Datta Bhattarai Yoo Kyung Lee Kyeung Hee Cho Hong Kum Lee Hyun Woung Shin 《Hydrobiologia》2006,560(1):417-423
Ten strains of marine bacteria (SCH0401–SCH0410) were isolated from Ayajin, the east coast of South Korea. In spectrophotometer
based chemotaxis assay the ethyl acetate extract (300 μg) of SCH0402 decreased the optical density (OD) of the motile target
strains SCH0401, SCH0402, SCH0407 and SCH0408 by two to six times when compared to control. Tributyltin oxide (TBTO) decreased
the OD of all target strains by only two times. The most active strain SCH0402 was identified as Shewanella oneidensis by using 16S rDNA gene sequence analysis. Similarly, the target motile strains SCH0401, SCH0402, SCH0407 and SCH0408 were
identified as Alteromonas marina, Shewanella oneidensis, Roseobacter gallaeciensis and Bacillus atrophaeus, respectively. The growth inhibition zone produced by the test bacterial extracts against the target strains were three to
eight times smaller when compared to that of TBTO. Even though, SCH0402 showed six times weaker antibacterial activity, the
repellent activity was three times stronger than TBTO. Therefore, the higher negative chemotactic activity would be better
to select eco-friendly antifouling compounds than the other antibacterial activities. 相似文献
14.
Ilkay Orhan Berrin Özçelik Sinem Aslan Murat Kartal Taner Karaoglu Bilge Şener Salih Terzioglu M. Iqbal Choudhary 《Phytochemistry Reviews》2007,6(1):189-196
Purpose of the present study was to evaluate antioxidant, antibacterial, antifungal, and antiviral activities of the petroleum
ether, chloroform, ethyl acetate and methanol extracts as well as the alkaloid fraction of Lycopodium clavatum L. (LC) from Lycopodiaceae growing in Turkey. Antioxidant activity of the LC extracts was evaluated by 1,1-diphenyl-2-picrylhydrazyl
(DPPH) radical-scavenging method at 0.2 mg/ml using microplate-reader assay. Antiviral assessment of LC extracts was evaluated
towards the DNA virus Herpes simplex (HSV) and the RNA virus Parainfluenza (PI-3) using Madin-Darby Bovine Kidney (MDBK) and Vero cell lines. Antibacterial and antifungal activities of the extracts
were tested against standard and isolated strains of the following bacteria; Escherichia coli, Pseudomonas aeruginosa, Proteus mirabilis, Acinobacter baumannii, Klebsiella pneumoniae, Staphylococcus aureus, Bacillus subtilis as well as the fungi; Candida albicans and C. parapsilosis. All of the extracts possessed noteworthy activity against ATCC strain of S. aureus (4 μg/ml), while the LC extracts showed reasonable antifungal effect. On the other hand, we found that only the chloroform
extract was active against HSV (16–8 μg/ml), while petroleum ether and alkaloid extracts inhibited potently PI-3 (16–4 μg/ml
and 32–4 μg/ml, respectively). However, all of the extracts had insignificant antiradical effect on DPPH. In addition, we
also analyzed the content of the alkaloid fraction of the plant by capillary gas chromatography-mass spectrometry (GC-MS)
and identified lycopodine as the major alkaloid. 相似文献
15.
Ahlam Sayout Aicha Ouarhach Reda Rabie Ilham Dilagui Nabila Soraa Abderrahmane Romane 《化学与生物多样性》2020,17(1)
The genus Lavandula is known for its different uses in traditional medicine. This study is interested in the chemical composition of Lavandulapedunculata subsp.atlantica (Braun‐Blanq. ) Romo as well as evaluating its antibacterial potential against multi‐resistant strains. The analysis of Lavandulaatlantica essential oil (LAEO) allows the identification of 47 components representing 93.6 % of all identified. The main constituent of LAEO was camphor (50.4 %), followed by fenchone (14.1 %) and camphene (5.6 %). The antibacterial assays revealed that LAEO was active against all the studied bacteria. A preliminary study of the relationship between certain terpenoids and antibacterial activity was also carried out in order to note the compound(s) that are responsible for LAEO's antibacterial activity. This study showed that the activity of the essential oil may be due to the presence of certain minor compounds such as carvone, considering the presence of the synergistic effect between the essential oil. 相似文献
16.
Human antimicrobial peptide CAP18/LL37 (hCAP18/LL37) was expressed in Pichia pastoris and its antibacterial activity was tested against pathogenic bacteria. The full length ORF of hCAP18/LL37 was cloned into
the pPICZaA vector followed by integration into the genomic AOX1 gene of P. pastoris. Agar diffusion assay demonstrated that the different hCAP18/LL37 transformants showed various antibacterial activities against
Staphylococcus aureus, Micrococcus luteus, and Salmonella gastroenteritis. The secreted form of hCAP18/LL37 exhibited its maximum activity after 72 h incubation with 2% methanol in MM media, not
in BMM. This result suggests that the yeast secreted expression system can be used as a production tool of antimicrobial peptides
for industrial or pharmaceutical application. 相似文献
17.
Dae-Sung Lee Min-Seung Kang Hye-Jin Hwang Sung-Hwan Eom Ji-Young Yang Myung-Suk Lee Won-Jae Lee You-Jin Jeon Jae-Sue Choi Young-Mog Kim 《Biotechnology and Bioprocess Engineering》2008,13(6):758-764
We have been attempting for some time to discover a compound evidencing antibacterial activity against methicillin-resistant
Staphylococcus aureus (MRSA). The dieckol isolated from Ecklonia stolonifera has been shown to exhibit antibacterial activity against methicillin-susceptible S. aureus (MSSA) and MRSA. The minimum inhibitory concentrations (MICs) of dieckol were determined in a range of 32 to 64 μg/mL against
standard MSSA and MRSA strains. Furthermore, dieckol clearly reversed the high-level ampicillin and penicillin resistance
of MRSA. The MICs of ampicillin against two standard strains of MRSA were dramatically reduced from 512 to 0.5 μg/mL in combination
with 1/4 MIC of dieckol (16 μg/mL). The fractional inhibitory concentration (FIC) indices of ampicillin and penicillin were
measured from 0.066 to 0.266 in combination with 8 or 16 μg/mL of dieckol against all tested MRSA strains, thereby suggesting
that dieckol-ampicillin or dieckol-penicillin combinations exert a synergistic effect against MRSA. The results of this study
indicate that dieckol, administered in combination with β-lactams, may prove effective in the treatment of MRSA infections.
Our finding may also contribute to the development of an alternative phytotherapeutic anti-MRSA agent. 相似文献
18.
Yang Yang Shunying Zhu Xiaoqiang Cai Lifei Tang Deqing Liu Jinghua Huang Guolin Zou 《Frontiers of Biology in China》2008,3(4):402-407
Essential oil of the subterranean part of Cacalia tangutica (Maxim.) Hand.-Mazz was analyzed by gas chromatography (GC)-mass spectrum (MS) technique in two different capillary columns
of different polarities. Thirty-one components were identified in the oil and the main compounds were α-zingiberene (13.49%), germacrene D (10.76%), α-pinene (8.54%), caryophyllene(Z-) (6.36%), linalool (6.16%), β-myrcene (4.89%), β-ocimene (Z-) (4.40%)and ocimenone(Z-) (3.58%). The antimicrobial activity of the oil was evaluated against 2 fungi and 12
bacteria including 6 clinically isolated strains using the agar disc diffusion and broth microdilution methods. The results
show that the oil presented a broad antimicrobial spectrum and had better antimicrobial activity against yeast and gram-positive
bacteria. The minimum inhibitory concentration values were 0.16–5.00 g/L and minimum bactericidal concentration values were
0.16–5.00 g/L.
__________
Translated from Journal of Wuhan University (Science Edition), 2007, 53 (2): 198–203 [译自: 武汉大学学报(理学版)] 相似文献
19.
Angelica del V. Chebel Adolfina R. Koroch Héctor R. Juliani Héctor R. Juliani Victorio S. Trippi 《In vitro cellular & developmental biology. Plant》1998,34(3):249-251
Summary Cultures ofMinthostachys mollis were established from nodal explants obtained under aseptic conditions. Explants were cultured on Murashige and Skoog (MS)
half-strength medium containing 6-benzyladenine (BA), and/or naphthaleneacetic acid (NAA). Optimum numbers of healthy shoots
were induced on media containing 0.05 μM NAA plus 2.2 μM BA; higher concentrations caused more hyperhydricity and less extension. Rooting was achieved on half-strength MS medium
with 0.05 μM NAA. Plantlets were acclimatized and successfully transferred to soil. Essential oil composition of the regenerated plants
were determined by gas chromatography/mass spectrometry and little differences were found in the essential oil composition
with the plant grown in the wild. 相似文献
20.
Antifouling Potential of Some Marine Organisms from India Against Species of Bacillus and Pseudomonas 总被引:2,自引:0,他引:2
Crude methanolic extracts of 37 marine organisms (16 species of flora, 21 species of fauna) were screened for antibacterial
properties against 5 strains of bacteria isolated from marine environments. Of these, 10 plant and 9 animal extracts exhibited
antibacterial activity against at least one bacterial strain. The extracts of 6 species were active against all the strains:
i.e., Stoechospermum marginatum (brown algae), Cymodocea rotundata (seagrass), Petrosia sp. and Psammaplysilla purpurea (sponges), Sinularia compressa (soft coral), and Cassiopeia sp. (jellyfish). Among the plants, Padina tetrastromatica (brown algae) extract exhibited significant activity (9–11-mm inhibition zone at 500 μg per 6-mm disc) against Bacillus pumilus and Pseudomonas vesicularis, while the extracts of Petrosia, Psammaplysilla, and Cassiopeia were strongly active (11–13-mm inhibition zone at 500 μg per 6-mm disc) against B. circulans and P. putida. It was further confirmed that the attachment of bacterial strains on glass slides was inhibited remarkably with increasing
concentrations of bioextracts of Petrosia sp. and Psammaplysilla purpurea. The present findings could form the basis for exploring the antibacterial potential of bioactive molecules from some of
the marine organisms that exhibited moderate to strong antibacterial properties. 相似文献