检查细菌呼吸方式和云霞生的一管多用鉴定培养基

自行研制一种细菌呼吸方式和运动性的一管多用鉴定细菌用培养基,可通过穿刺接种,３７℃,２４－４８ｈ培养后在同一支高层培养基判定好氧性,厌氧性及运动性,经过接种临床常上菌属１２０个菌株的试验结果表明,该培养基有很好的实用性,其本方组合合理,材料易得,成本不高,质量稳定,使用简单,判定容易,可以省去厌氧菌２检查器材的试剂,有助于提高细菌鉴定工作效率 质量。     

检查细菌呼吸方式和运动性的一管多用鉴定培养基

自行研制一种检查细菌呼吸方式和运动性的一管多用鉴定细菌用培养基,可通过穿刺接种,37℃24～48h培养后在同一支高层培养基判定好氧性、厌氧性及运动性,经过接种临床常见40个菌属120个菌株的试验结果表明,该培养基有很好的实用性。其配方组成合理,材料易得,成本不高,质量稳定,使用简单,判定容易,可以省去厌氧菌培养检查器材和试剂,有助于提高细菌鉴定工作效率和质量。     

蛋黄琼脂培养基用于白喉杆菌的培养

白喉,是一种严重影响人民健康的传染病。白喉杆菌的分离培养,是准确诊断以及进行流行病学调查的重要手段。自从吕氏血清斜面、白氏鸡蛋斜面及血碲盐平板等培养基应用以来,对白喉杆菌的检查和研究,起了重要的推动作用和良好效果。在上述培养基的基础上,我们加以简化,制成蛋黄琼脂培养基,现介绍如下:     

七种微生物菌株的保藏结果

我们试用5种方法保藏了7种微生物菌株,现将保藏结果简报如下: 一、保藏方法1.斜面转接低温保藏:将菌种接种在适宜的斜面培养基上,在适温下培养至生长良好     

一株耐高温菌CICC 10853的分离和鉴定

从斜面培养基中分离到一株耐高温菌CICC 10853,对该菌株的分类地位进行了研究。通过形态学、生理生化特征、16S r RNA和rec N基因序列分析的多相鉴定技术,将其鉴定为噬热地芽胞杆菌(Geobacillus thermoleovorans),为后续对该菌的生物学功能研究奠定基础。     

橡皮塞试管斜面保藏某些细菌和酵母菌的效果

我们用橡皮塞试管斜面保藏过一批细菌和酵母菌菌株。现将保藏效果报告如下。一、细菌用牛肉膏蛋白胨琼脂培养基保藏26种70株细菌,结果见表1。     

佛罗里达平菇的生物学特性

本文研究我国从西德引进的佛罗里达平菇菌株的生理特性,包括斜面菌丝生长状况,菌丝生长所需的适宜温度、光照、空气、pH以及理想的碳源和氮源。在棉子壳培养基上生长的子实体的营养成分及其毒性鉴定。为大面积推广应用提供了科学依据。     

黑木耳干耳基组织分离纯菌种技术

笔者经过较长时间的制种实践，反复试验，探索出了一套黑木耳干耳基组织分离纯菌种的技术。此法取材简便，不受季节的限制，一年四季皆可分离，成功率高，且菌种纯度高。１母种分离培养基采用综合培养基，加入抑菌的青霉素或链霉素，浓度为４０×１０－６。调制斜面培养基...     

白软肉多孔菌的人工培养

多孔菌属的菌肉多木质化,少呈软肉质。白软肉多孔菌(Overholts 1977)肉质肥厚,海绵状,幼时鲜嫩,干后香味亦浓,干品经水浸后即变软可食。作者在云南碧罗雪山海拔2600m处的冷杉(Abies sp.)倒木上采到和人工培养成功。 材料和方法 该菌种自1978年从野外分离出菌丝后在马铃薯葡萄糖琼脂培养基上培养,用组织分离法获得纯菌种。每二个月转管一次,低温保存已逾5年。 适合菌丝体生长的培养基和温度:以三种培养基制成斜面,接菌后分别置于不同温度条件下,经过一定天数培养分别测量菌丝体在斜面培养基上的长度。三种培养基分别     

新霉素产生菌Streptomyces fradiae高效价菌株的选育

我们试验了常用的诱变因素紫外线及氮芥子气对新霉菌所引起的死亡率及变异率的影响,其中以氮芥子气引起的变异率较大。从单一氮芥子气处理中,获得61—7—12菌株此原种产生抗菌素的能力有明显提高。经初筛及复试,在摇瓶发酵中较U1—19菌株提高100—120％。61一7一12菌株在粽合斜面培养基及几种合成斜面培养基上的生长速度厦菌落形态与原种均有明显的区刖：生长极为缓慢,在28℃时绠培养10一12天;孢手颜色较U1—19菌株稍深。在对不同碳源利用的生理特性比较中,67—12菌株亦与Ul一19菌株不同。     

Thin-Layer Lactose Agar for Pollen-Tube Culture of Tradescantia To Enhance Planar Distribution of Chromosomes

Mature pollen grains of Tradescantia paludosa were sown on a thin layer of lactose-agar medium at 38-39 C. After 16 hr incubation, slides were fixed in aceto-alcohol (1:3; for 1-3 hr, hydrolyzed in 1 N HC1 at 60 C, and treated with water at 65 C. Delamination of the upper layer of medium was accomplished in cold water. The delaminated upper layer of solidified medium containing most of the ungerminated pollen and underdeveloped pollen tubes was flushed off with running water. The remaining single layer of pollen tubes was flattened and firmly affixed to the slide by pressing under a coverglass on a hot surface at 80 C. The quick-freeze technique was used to remove the coverglass prior to staining, dehydration and permanent mounting. Preparations made according to this procedure gave a planar chromosome distribution and approximately 160 analyzable metaphase figures per slide, thus facilitating aberration analysis and autoradiography. Comparative studies on the effectiveness of colchicine, Colcemid, and acenapthene in arresting mitotic nuclei at metaphase indicated that Colcemid was more effective than the others, but that it caused deformities of the metaphase chromosomes and induced chromatid breaks at a concentration of 0.2 mg/ml or higher. Colchicine is a more favorable chemical than the other two.     

Graded Impregnation of Nervous Tissue Stained by the Golgi Procedure

A simple technique is described by which retinal whole mounts or slices of brain can be impregnated by the Golgi procedure in a graded manner. The unfixed tissue is laid on a glass slide and covered with a layer of Telfa gauze which is tied in place. Following fixation, the progress of staining, which begins beneath holes in the gauze, is followed by direct observation through the slide. The tissue is impregnated or reimpregnated until the desired results are obtained.     

Surface Printing of Plant Leaves for Phylogenetic Studies

A solution of plastic consisting of toluene, 720 ml; methanol, 180 ml; ethyl cellulose (Ethocel, standard 7 CPS), 250 gm; and Dow resin 276 V-2, 75 gm is applied to a leaf surface which has been dampened with toluene. The plastic is spread to a thin film with the edge of a card and allowed to dry. After drying, the plastic may be peeled from the leaf surface and either stored dry in a small envelope or mounted permanently on a microscope slide. Permanent mounts are prepared by placing a small section of the peel from the upper and lower surfaces of the leaf on a microscope slide and covering with a No. 1 cover glass. A small spot of balsam on each corner of the cover glass secures the glass in position. This air mount has proved to be superior to water, glycerol or balsam mounts. Fresh leaves are washed with a mild detergent before application of the plastic. Herbarium specimens are soaked in water overnight to restore the leaf to a semiturgid condition. Five species of different plant families have been illustrated to show the diagnostic features of the surface of the cuticle. An isolated layer of epidermal cells obtained by chemical maceration permitted cell and imprint comparison. The remarkable amount of detail shown by the prints is an aid for phylogenetic studies and may make the recognition of fossil cuticles possible.     

Erratum

A solution of plastic consisting of toluene, 720 ml; methanol, 180 ml; ethyl cellulose (Ethocel, standard 7 CPS), 250 gm; and Dow resin 276 V-2, 75 gm is applied to a leaf surface which has been dampened with toluene. The plastic is spread to a thin film with the edge of a card and allowed to dry. After drying, the plastic may be peeled from the leaf surface and either stored dry in a small envelope or mounted permanently on a microscope slide. Permanent mounts are prepared by placing a small section of the peel from the upper and lower surfaces of the leaf on a microscope slide and covering with a No. 1 cover glass. A small spot of balsam on each corner of the cover glass secures the glass in position. This air mount has proved to be superior to water, glycerol or balsam mounts. Fresh leaves are washed with a mild detergent before application of the plastic. Herbarium specimens are soaked in water overnight to restore the leaf to a semiturgid condition. Five species of different plant families have been illustrated to show the diagnostic features of the surface of the cuticle. An isolated layer of epidermal cells obtained by chemical maceration permitted cell and imprint comparison. The remarkable amount of detail shown by the prints is an aid for phylogenetic studies and may make the recognition of fossil cuticles possible.     

Modification of the anal tape method for detection of pinworms in rodents

A modification of Graham's anal tape technique for detection of oxyurid worm eggs involves a coverslip or microscope slide covered with a thin layer of adhesive. The worm eggs stick to the adhesive and can be directly observed under the microscope.     

Protein profiled features patterned via confocal microscopy

Protein patterns were printed using conventional microlithographic materials in a bilayer arrangement and unconventional exposure tools. The bilayer resist stack consisted of a lower poly(tert-butyl methacrylate) layer and an upper diazonaphtoquinone/novolak layer. The protein features were printed in either 'contact printing', or 'step and repeat' mode. The latter printing mode can be managed in a flow-cell consisting of a standard microscope slide and cover slip, spaced apart by about 20 microm, as follows: (i) the exposure step is carried out in the cell using focused 488 nm beam of a confocal laser scanning microscope; (ii) the development step is performed by flowing the photoresist developer through the cell; (iii) the selective deposition of the protein (FITC-labelled avidin) is achieved via the flow of the protein solution through the cell until a desired contrast has been reached; (iv) the control of the process is assured using on-line monitoring of the photo-activated red fluorescence of the developing resist layer, and of the green fluorescence of the FITC-protein patterns, respectively. The protein printing technique uses equipment routinely available in biological laboratory. The 'step and repeat' patterning yields high and controllable resolution. The process can be applied in the fabrication of medical microanalysis devices.     

A Numbered-Grid Locator Slide for Relocating Microscopic Fields

Four strips of drawing paper, each 22 × 30 cm, are taken. Numbers are typed from a typewriter with elite type face in such a way that, when pasted side by side along their lengths on another sheet of drawing paper, they yield a 88 × 30 cm master copy carrying numbers commencing from 1001 at top left and ending with 6680 at bottom right, each number preceded and succeeded by a dot. The upper and lower edges of this rectangle containing these figures are then extended by lines 2 cm each to the left and the ends jointed to make up a 90 × 30 cm master copy from which a 3 × 1 inch (7.5 × 2.5 cm) slide is prepared by photographic reduction. To use this slide, the microscopic field is found, the slide carrying the tissue is exactly replaced by the locator slide, and the number recorded. For subsequent reference, the procedure is reversed by first finding the number and then replacing the locator slide by the one bearing the tissue     

非线性模型在蛋白质薄膜厚度测定中的应用

分子通过测定蛋白质薄膜厚度变化而定量地研究生物分子间的相互作用,探讨了基于光学干涉光的薄膜厚度的测量方法,借助于在玻璃基底表面沉积的聚苯乙烯薄膜对噪声的抑制,使用非线性回归模型对生物传感器的检测信号进行了分析。通过反射干涉光谱光测定到乙型肝炎表面抗原在聚苯乙烯－玻璃表面的吸附使薄膜厚度增加了３．３ｎｍ。随着５μｇ／ｍｌ,１０μｇ／ｍｌ,２０μｇ／ｍｌ,３０μｇ／ｍｌ和５０μｇ／ｍｌ浓度的乙型肝炎表     

Leptospirotic etiology in pulmonary and upper respiratory tract pathology

A number of 3,200 febrile patients who presented upon admission to hospital primary pulmonary or upper respiratory tract impairment either as single forms of manifestation or associated to other syndromes were tested. The cases were screened by the rapid slide agglutination reaction with heat inactivated Patoc antigen and leptospirotic etiology was confirmed by the ultramicroscopic agglutination reaction with 18 live circulating pathogenic antigens. 64 leptospirosis cases with pulmonary impairment were confirmed and in 52 cases the upper respiratory tract was involved. Particular aspects of leptospirosis with pulmonary impairment: 71.8% of cases had a clinical diagnosis of interstitial pneumonia; 89% of cases presented important chest x-ray modifications; in an approximately equal number of cases the pulmonary involvement was the single manifestation or was associated with other syndromes; icterohaemorrhagiae, wolffi and pomona were the frequently encountered infecting serotypes. Particular aspects for leptospirosis involving the upper respiratory tract: 84.6% of cases had a clinical diagnosis of acute rhino-pharyngotracheitis; in 86.5% of cases the upper respiratory tract impairment was the single feature; the infecting serotypes were in decreasing order of frequency as follows: icterohaemorrhagiae, pomona, wolffi, canicola, grippotyphosa.     

Positioning, displacement, and localization of cells using ultrasonic forces

This paper presents a method and a device to position and displace cells. The cells are suspended in a fluid layer trapped between the device and an arbitrary surface such as an object slide or a wafer. The device vibrates at ultrasonic frequencies causing a pressure field in the fluid layer. This pressure field results in a force-field capable of positioning cells. Depending on the way in which the device is excited a 2-D or 3-D force-field can be generated, positioning the cells in lines or points respectively. Furthermore, it is possible to subsequently displace the cells with micrometer accuracy. This has been demonstrated using HL60 and MCF10A cells, and can be achieved without causing damage to the cells.