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1.
昆虫信息素结合蛋白的研究概况   总被引:1,自引:0,他引:1  
朱彬彬  姜勇  雷朝亮 《昆虫知识》2005,42(3):240-243
在昆虫感受信息素的嗅觉反应中,信息素结合蛋白发挥了重要的作用。它作为脂溶性信息素的溶剂和载体,在亲水性淋巴液中起着运载信息素和使之失活的双重作用。由于它在昆虫识别信息素物质中起着重要的作用,近1 0年来,国内外对其进行了广泛、深入的研究。文章从信息素结合蛋白的生化特点、表达情况、代谢以及生理功能等方面的概况进行综述。  相似文献   

2.
实夜蛾属和铃夜蛾属昆虫性信息素通讯系统的研究进展   总被引:3,自引:3,他引:0  
实夜蛾属Heliothis和铃夜蛾属Helicoverpa昆虫的性信息素通讯系统主要包括雌蛾的性信息素合成和雄蛾对性信息素接收两个方面,每方面都有分子、细胞、系统水平上进行协同作用的生物过程。性信息素生物合成激活肽(PBAN)与其受体作用,启动信号转导系统,从而激活合成性信息素的酶系统来合成性信息素,利用化学和生物测定的方法鉴定出具有诱蛾活性的性信息素腺体组分及行为功能;性信息素分子与性信息素结合蛋白(PBP)的复合体同受体相互作用,启动信号转导系统,诱导产生神经信号,从而引起一系列性行为反应。这些生物过程受到各种内部和外部因素的影响。  相似文献   

3.
《环境昆虫学报》2014,(4):585-596
本文从螟蛾总科昆虫性信息素分泌腺体及分泌节律、性信息素受体及结合蛋白、性信息素的结构及组成特点、近缘种相似性及种内变异等方面综述了螟蛾总科昆虫性信息素的研究进展。最后对需要进一步研究的问题提出了展望。  相似文献   

4.
昆虫聚集信息素   总被引:14,自引:0,他引:14  
姜勇  雷朝亮  张钟宁 《昆虫学报》2002,45(6):822-832
昆虫聚集信息素是昆虫重要的信息化学物质之一,对昆虫的聚集行为有重要意义。近三十年来,国外鉴定了多种昆虫聚集信息素,主要成分为一些烃、醇、醛、酮、酯、酸、酸酐、胺以及腈类化合物,但其在有害生物可持续治理中的应用潜能尚未充分利用;昆虫聚集信息素的来源多样,除蛹外,多个虫态均有聚集信息素释放,有些学者甚至把一些寄主释放的挥发物作为聚集信息素的组分;同种昆虫,不同生理状态,其聚集信息素可以完全不同或同一信息化学物质的功能不同;但是,并非所有昆虫的聚集行为均为聚集信息素调节,利他素、性信息素以及报警信息素等其它信息化学物质均能导致一些昆虫的聚集。本文综述了5目17科55种昆虫的聚集信息素。  相似文献   

5.
翁琛  张林雅  赵磊  付余霞  罗晨  李红亮 《昆虫学报》2013,56(10):1110-1116
【目的】研究中华蜜蜂Apis cerana cerana信息素结合蛋白ASP1与蜜蜂信息素及某些植物挥发物分子的结合功能。【方法】构建中蜂ASP1的原核表达载体, 对其进行重组蛋白的诱导表达和分离纯化, 并得到具有生化活性的中蜂ASP1重组蛋白, 最后以1-NPN作为荧光报告探针, 通过荧光竞争结合实验研究中蜂重组ASP1蛋白与蜜蜂信息素及其他气味分子的结合功能。【结果】在22种潜在信息气味物质中, 有7种与中蜂ASP1有较强的结合能力, 能将1-NPN的相对荧光强度降至50%以下。其中发现蜂王信息素两种成分对 羟基苯甲酸甲酯和香草醇的竞争能力最强, 可分别引起1-NPN相对荧光值下降99.31%和95.50%, 解离常数KD分别为13.39和98.44 μmol/L; 而与除蜂王信息素外的其他信息素如幼虫信息素和工蜂信息素等分子均不结合。此外中蜂ASP1对于水杨酸甲酯、 苯乙醛、 3, 4-二甲基苯甲醛4-烯丙基藜芦醚和β-紫罗兰酮等5种植物挥发物质能产生强度不一的结合。【结论】中蜂信息素结合蛋白ASP1对蜂王信息素具有非常强的特异性, 同时也能结合某些植物挥发性气味分子, 暗示中蜂ASP1是一种以蜂王信息素识别为主要功能、 植物挥发物识别为次要功能的多功能信息素结合蛋白。  相似文献   

6.
郑凯迪  杜永均 《昆虫学报》2012,55(9):1093-1102
蛾类昆虫性信息素受体首先从烟芽夜蛾Heliothis virescens和家蚕Bombyx mori中鉴定出来, 到目前为止已经克隆得到了19种蛾类昆虫的几十种性信息素受体基因, 并且这些基因在系统发育树中聚成一个亚群。性信息素受体从蛾类蛹期开始表达, 主要表达在雄性触角的毛形感器中, 少部分受体在雌性触角、 雄性触角其他感器以及身体其他部位中也有表达。大部分蛾类性信息素受体的配体并不是单一的, 而是能够对多种性信息素组分有反应, 部分性信息素受体还能够识别性信息素以外的其他物质, 还有一部分性信息素受体的识别配体目前尚不清楚。另外发现在雌性蛾类触角中也存在一些嗅觉受体能够识别雄性分泌的性信息素。在蛾类性信息素受体与性信息素识别的过程中, 性信息素结合蛋白不仅能够特异性地运送配体到嗅觉神经元树状突上, 还能够提高性信息素与性信息素受体之间的结合效率。另外, OrCo类受体与性信息素受体共表达在嗅觉神经元中, 在蛾类性信息素受体与配体的识别过程中扮演了重要角色。但是蛾类信息素对神经元刺激的终止并非由性信息素受体控制, 而是由细胞中的气味降解酶等其他因子调控。蛾类性信息素受体研究中还有很多疑问需要解答, 其过程可能比我们想象的更为复杂。  相似文献   

7.
植物源挥发物对昆虫信息素的增效作用及其增效机制   总被引:4,自引:0,他引:4  
植物源挥发物和昆虫信息素是昆虫的重要信息物质,二者协同作用以调节昆虫的行为.通过增加触角电位、信息素接收神经元动作电位和脉冲频率,特异性植物源挥发物能显著增强昆虫性信息素和聚集信息素的引诱力.这种对昆虫信息素的增效作用受昆虫体内的章鱼胺及其受体介导.特异性植物源挥发物和章鱼胺受体结合,降低性信息素接收神经元对性信息素的反应阈值,增强性信息素接收神经元敏感性.这可能是植物源挥发物对昆虫信息素具有增效作用的主要机制.  相似文献   

8.
蛾类性信息素研究进展   总被引:14,自引:0,他引:14  
韦卫  赵莉蔺  孙江华 《昆虫学报》2006,49(5):850-858
蛾类是昆虫性信息素中研究较多的类群,已有565种的性信息素成份被鉴定,其相关研究在害虫监测和防治上得到实际应用的同时,已涉及到生态、生化、遗传等诸多方面,特别是在物种多样性的化学表达及物种的生殖隔离现象的分子水平的表达上,提供了研究的典范。本文将介绍蛾类性信息素的多样性、性信息素化学结构鉴定、微量成分的作用、合成性信息素的利用、性信息素生物合成酶、性信息素生产的调节机制及性信息素的感受机制等方面的研究现状和存在的问题。  相似文献   

9.
昆虫的性信息素及其应用   总被引:18,自引:0,他引:18  
较系统地介绍了昆虫性信息素领域取得的成就,包括昆虫性信息素的种类、化学、生物学、结构鉴定及信息应用等,并进行了展望。  相似文献   

10.
绿盲蝽气味结合蛋白AlucOBP7的表达及气味结合特性   总被引:4,自引:0,他引:4  
气味结合蛋白(odorant binding proteins,OBPs)在昆虫嗅觉识别中起着重要的作用,尤其是在运输外界脂溶性气味分子通过嗅觉感器淋巴液到达嗅觉受体(olfactory receptors,ORs)的过程中发挥关键作用。明确OBPs在昆虫同外界进行信息交流过程中的作用有利于阐明昆虫嗅觉识别的机制,同时可为利用干扰昆虫嗅觉识别来进行害虫防治奠定理论基础。本研究克隆了一个绿盲蝽Apolygus lucorum(Meyer-Dür)气味结合蛋白AlucOBP7基因(GenBank登录号:JQ675724),并进行了原核表达,以1-NPN为荧光探针采用荧光竞争结合实验研究了AlucOBP7蛋白和10种棉花挥发物及6种性信息素类似物的结合能力。结果表明:在10种棉花挥发物中,AlucOBP7能够和2-己酮及水杨酸甲酯有效结合,结合常数分别为55.13μmol/L和28.26μmol/L。在6种盲蝽性信息素类似物中,4-氧代-反-2-己烯醛和AlucOBP7具有较强的结合能力,结合常数为23.14μmol/L。丁酸乙酯、丁酸丁酯及己酸己酯也能够和AlucOBP7有效结合,但结合能力中等,结合常数分别为30.58,39.26和35.81μmol/L。初步推测,AlucOBP7可能是绿盲蝽性信息素结合蛋白(pheromone binding proteins,PBPs),并在感受性信息素和植物挥发物的过程中发挥双重功能。  相似文献   

11.
Once captured by the antenna, the male‐specific pheromone 11‐cis vaccenyl acetate (cVA) binds to an extracellular binding protein called LUSH that undergoes a conformational shift upon cVA binding. The stable LUSH–cVA complex is the activating ligand for pheromone receptors present on the dendrites of the aT1 neurones, comprising the only class of neurones that detect volatile cVA pheromone. This mechanism can explain the single molecule sensitivity of pheromone detection. The receptor that recognizes activated LUSH consists of a complex of several proteins, including Or67d, a member of the tuning odourant receptor family, Orco, a co‐receptor ion channel, and SNMP, a CD36 homologue that may be an inhibitory subunit. In addition, genetic screens and reconstitution experiments reveal additional factors that are important for pheromone detection. Identification and functional dissection of these factors in Drosophila melanogaster Meigen should permit the identification of homologous factors in pathogenic insects and agricultural pests, which, in turn, may be viable candidates for novel classes of compounds to control populations of target insect species without impacting beneficial species.  相似文献   

12.
Odorant‐binding proteins (OBPs) and chemosensory proteins (CSPs) are regarded as carriers of pheromones and odorants in insect chemoreception. These proteins are typically located in antennae, mouth organs and other chemosensory structures; however, members of both classes of proteins have been detected recently in other parts of the body and various functions have been proposed. The best studied of these non‐sensory tasks is performed in pheromone glands, where OBPs and CSPs solubilise hydrophobic semiochemicals and assist their controlled release into the environment. In some cases the same proteins are expressed in antennae and pheromone glands, thus performing a dual role in receiving and broadcasting the same chemical message. Several reports have described OBPs and CSPs in reproductive organs. Some of these proteins are male specific and are transferred to females during mating. They likely carry semiochemicals with different proposed roles, from inhibiting other males from approaching mated females, to marking fertilized eggs, but further experimental evidence is still needed. Before being discovered in insects, the presence of binding proteins in pheromone glands and reproductive organs was widely reported in mammals, where vertebrate OBPs, structurally different from OBPs of insects and belonging to the lipocalin superfamily, are abundant in rodent urine, pig saliva and vaginal discharge of the hamster, as well as in the seminal fluid of rabbits. In at least four cases CSPs have been reported to promote development and regeneration: in embryo maturation in the honeybee, limb regeneration in the cockroach, ecdysis in larvae of fire ants and in promoting phase shift in locusts. Both OBPs and CSPs are also important in nutrition as solubilisers of lipids and other essential components of the diet. Particularly interesting is the affinity for carotenoids of CSPs abundantly secreted in the proboscis of moths and butterflies and the occurrence of the same (or very similar CSPs) in the eyes of the same insects. A role as a carrier of visual pigments for these proteins in insects parallels that of retinol‐binding protein in vertebrates, a lipocalin structurally related to OBPs of vertebrates. Other functions of OBPs and CSPs include anti‐inflammatory action in haematophagous insects, resistance to insecticides and eggshell formation. Such multiplicity of roles and the high success of both classes of proteins in being adapted to different situations is likely related to their stable scaffolding determining excellent stability to temperature, proteolysis and denaturing agents. The wide versatility of both OBPs and CSPs in nature has suggested several different uses for these proteins in biotechnological applications, from biosensors for odours to scavengers for pollutants and controlled releasers of chemicals in the environment.  相似文献   

13.
Pheromone‐binding proteins (PBPs) are thought to bind and transport sex pheromones onto the olfactory receptors on the dendrite membrane of olfactory neurons, and thus play a vital role in sex pheromone perception. However, the function of PBPs has rarely been demonstrated in vivo. In this study, two PBPs (PBP1 and PBP3) of Chilo suppressalis, one of the most notorious pyralid pests, were in vivo functionally characterized using insects with the PBP gene knocked out by the CRISPR/Cas9 system. First, through direct injection of PBP‐single guide RNA (sgRNA)/Cas9 messenger RNA into newly laid eggs, a high rate of target‐gene editing (checked with polled eggs) was induced at 24 h after injection, 21.3% for PBP1‐sgRNA injected eggs and 19.5% for PBP3‐sgRNA injected eggs. Second, by an in‐crossing strategy, insects with mutant PBP1 or PBP3 (both with a premature stop codon) were screened, and homozygous mutants were obtained in the G3 generation. Third, the mutant insects were measured for electroantennogram (EAG) response to female sex pheromones. As a result, both PBP mutant males displayed significant reduction in EAG response, and this reduction in PBP1 mutants was higher than that in PBP3 mutants, indicating a more important role of PBP1. Finally, the relative importance of two PBPs and the possible off target effect induced by sgRNA‐injection are discussed. Taken together, our study provides a deeper insight into the function of and interaction between different PBP genes in sex pheromone perception of C. suppressalis, as well as a valuable reference in methodology for gene functional study in other genes and other moth species.  相似文献   

14.
Pheromone‐binding proteins (PBPs) play important roles in the information exchange between insect sexes, specifically in the process of transporting fat‐soluble odour molecules from the external environment to olfactory receptors through the olfactory sensillum lymph. The PBP functions in this process may explain the sex pheromone identification mechanism used by insects, laying a theoretical foundation for the prevention and control of pests by interfering with olfactory recognition. In the present study, a PBP gene of Cyrtotrachelus buqueti (GenBank accession number: KU845733) is cloned for prokaryotic expression. Using N‐phenyl‐1‐naphthylamine as the fluorescent probe in a competitive binding assay, the ability of CbuqPBP1 to bind 12 sex pheromone analogues and three volatiles of Neosinocalamus affinis shoots is examined. Of the 12 C. buqueti sex pheromone analogues, dibutyl phthalate gives the greatest displacement (inhibitory constant value of 11.1 μm ), whereas the other sex pheromone components show much smaller displacements. Consistent with other PBPs, the three plant volatiles (linalool, benzaldehyde and indole) show only a limited displacement of CbuqPBP1. However, the binding abilities of 1 : 1 ratios of each of the three plant volatiles with dibutyl phthalate show increases of 62.3%, 65.1% and 51.7% over the binding abilities of the three plant volatiles alone. CbuqPBP1 has dual roles in the processes of sensing sex pheromones and plant volatiles.  相似文献   

15.
Male moths respond to conspecific female-released pheromones with remarkable sensitivity and specificity, due to highly specialized chemosensory neurons in their antennae. In Antheraea silkmoths, three types of sensory neurons have been described, each responsive to one of three pheromone components. Since also three different pheromone binding proteins (PBPs) have been identified, the antenna of Antheraea seems to provide a unique model system for detailed analyzes of the interplay between the various elements underlying pheromone reception. Efforts to identify pheromone receptors of Antheraea polyphemus have led to the identification of a candidate pheromone receptor (ApolOR1). This receptor was found predominantly expressed in male antennae, specifically in neurons located beneath pheromone-sensitive sensilla trichodea. The ApolOR1-expressing cells were found to be surrounded by supporting cells co-expressing all three ApolPBPs. The response spectrum of ApolOR1 was assessed by means of calcium imaging using HEK293-cells stably expressing the receptor. It was found that at nanomolar concentrations ApolOR1-cells responded to all three pheromones when the compounds were solubilized by DMSO and also when DMSO was substituted by one of the three PBPs. However, at picomolar concentrations, cells responded only in the presence of the subtype ApolPBP2 and the pheromone (E,Z)-6,11-hexadecadienal. These results are indicative of a specific interplay of a distinct pheromone component with an appropriate binding protein and its related receptor subtype, which may be considered as basis for the remarkable sensitivity and specificity of the pheromone detection system.  相似文献   

16.
【目的】为了更好地了解性信息素结合蛋白(pheromone binding proteins,PBPs)在桃蛀螟Conogethes punctiferalis(Guenée)嗅觉识别过程中的作用,明确其与配体化合物的结合特性。【方法】本研究利用RT-PCR结合RACE方法克隆了桃蛀螟一个性信息素结合蛋白基因;采用Real-time PCR方法分析了该蛋白在桃蛀螟不同发育阶段及雌雄蛾间的表达差异;利用荧光竞争结合实验对Cpun-PBP1蛋白与16种配基化合物的结合特性进行了分析。【结果】克隆了一个桃蛀螟性信息素结合蛋白基因,命名为Cpun-PBP1(Gen Bank登录号:KP027486)。Cpun-PBP1开放阅读框全长510 bp,编码169个氨基酸,预测分子量为19.12 k Da,等电点为5.09,N-末端包括由起始位置开始的30个氨基酸组成的信号肽。蛋白特征分析显示,该氨基酸序列具有昆虫气味结合蛋白的典型特征,即含有6个保守的半胱氨酸残基。Cpun-PBP1在桃蛀螟成虫阶段表达量最高,且几乎全部在触角中表达,卵期微量表达,幼虫期和蛹期均不表达。通过构建Cpun-PBP1原核表达载体,诱导并获得Cpun-PBP1重组蛋白。荧光竞争结合实验对2种性信息素组分和14种寄主植物挥发物的结合力发现,Cpun-PBP1不但能有效地与桃蛀螟性信息素组分(顺-10-十六碳烯醛和十六醛)结合,结合常数分别为7.32和9.39μmol/L;还能与8种寄主植物挥发物有效结合;其中,与莰烯的结合能力最强,结合常数为3.76μmol/L。【结论】根据这些结果,我们推测Cpun-PBP1在桃蛀螟感受性信息素和寄主植物挥发物的过程中发挥着双重作用。  相似文献   

17.
灰翅夜蛾属昆虫的性信息素研究概况   总被引:8,自引:0,他引:8  
本文从雌蛾性信息素组分鉴定、雄蛾对性信息素的行为反应和性信息素的应用3个方面,综述了灰翅夜蛾属昆虫性信息的研究进展,并展望了此属昆虫性信息素的研究前景。  相似文献   

18.
PBAN及其对昆虫性外激素的调控   总被引:1,自引:0,他引:1  
张丛  王琛柱 《昆虫知识》2001,38(5):326-330
本文综述了性外激素生物合成激活神经肽 (PBAN)及其对昆虫尤其是鳞翅目昆虫性外激素产生的调控 ,包括PBAN的结构、产生、转运、作用方式及保幼激素和蜕皮激素对性外激素合成的作用 ,并展望了未来的研究方向。  相似文献   

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