共查询到19条相似文献,搜索用时 137 毫秒
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竹黄的分离培养研究进展 总被引:11,自引:1,他引:10
竹黄因其主要活性成分竹红菌素在医药、食品及农业领域的广泛应用前景而日渐受到广大研究者的关注。本文综述了竹黄的生物学性状、无性型的分离、及固体和液体培养的研究现状。探讨了人工培养的未来发展。 相似文献
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蚜虫与其胞内共生细菌的相互作用 总被引:3,自引:0,他引:3
蚜虫—巴克纳氏菌之间是一种典型的互利共生关系,两者相互依存,缺少一方,另一方便不能生存。研究表明,共生细菌能为寄主蚜虫提供必需氨基酸和维生索,并对寄主具有一些非营养功能,如促进蚜虫传播循环性病毒等。寄主蚜虫则是为共生菌提供一个合适的生存场所,并对共生菌的生长和繁殖进行调控。现代分子生物学技术从基因水平证明了蚜虫与共生菌的相互依赖性。 相似文献
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《基因组学与应用生物学》2016,(4)
为了提高竹黄菌(Shiraia bambusicola P.Hennings)中原生质体用于遗传转化时的转化效率,研究竹黄菌原生质体制备及再生的最佳条件,建立竹黄菌原生质体的高效遗传转化体系。本研究以竹黄菌为材料,采用正交试验分析方法,对影响竹黄菌原生质体制备及再生的主要因素,不同酶解时间、酶解温度、渗透压稳定剂及菌龄、不同再生培养基等进行了系统的研究。结果表明,以0.6 mol/L Na Cl为稳渗剂,用组合酶(质量分数1%裂解酶和质量分数1.5%崩溃酶按4:6体积比混合)在33℃条件下对菌龄为6 d的菌丝体酶解2 h,原生质体产量最高,达到10.57×10~6个/m L;以0.6 mol/L蔗糖为稳渗剂,用组合酶(质量分数1%裂解酶和质量分数1.5%崩溃酶按4:6体积比混合)在29℃条件下对菌龄为4 d的菌丝体酶解2 h,原生质体再生率最高,为0.293%;最适合竹黄菌原生质体再生的培养基是TB3再生培养基。 相似文献
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先对不同产地采集的竹黄菌进行筛选,得到优产竹红菌素的菌株,然后采用单因子和3因素3水平正交试验法对竹红菌素液体发酵条件进行优化,在优化培养基的基础上,选用不同浓度的Cr3+、Fe3+、Cu2+和Ca2+对竹红菌素进行离子调控研究。结果表明:从休宁所采集的菌株不仅生长速度最快,发酵所产的竹红菌素含量也最高;竹红菌素最佳发酵碳源是葡萄糖,最佳发酵氮源是硝酸钠,最佳培养基组合为2%葡萄糖,0.2%硝酸钠,pH7.5;Cr3+和Fe3+浓度为0.005%时竹红菌素含量均最高;0.05%的Ca2+最有利于竹红菌素的分泌;Cu2+为0.03%时竹红菌素含量达到最大值。 相似文献
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1,5-Dihydroxy-3-methoxy-7-methylanthracene-9,10- dione (shiraiarin) is a kind of antitumor and antibacterial anthraquinone, and was produced for the first time from the submerged fermentation of Shiraia bambusicola, as confirmed by ESI-MS and NMR. The production of shiraiarin was significantly influenced when varying the carbon source, and a high amount of shiraiarin was only achieved when using lactose. The production of shiraiarin was also stimulated when using NaNO3 as the nitrogen source, whereas other nitrogen sources inhibited its production. Shiraiarin was formed during the stationary phase with a pH value higher than 8. The production of shiraiarin was inhibited by sporulation. 相似文献
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华东竹黄菌不同居群遗传分化的RAPD分析 总被引:2,自引:0,他引:2
为了解竹黄地理居群间的遗传分化,本研究采用随机引物扩增多态性DNA分子标记技术对江苏、安徽和浙江3省的8个居群共32个竹黄样本进行了遗传多样性分析.从50个RAPD引物中筛选得到了5个随机引物,对供试材料的DNA进行扩增,共检测出77个位点,其中多态性位点52个,多态性位点比率为67.53%.UPGMA聚类分析结果表明,这8个居群分为三类:安吉居群、临安居群、宜兴居群、广德居群和泾县居群聚为一类;宁国居群和休宁居群聚为一类;淳安居群单独为一类.遗传多样性分析表明8个竹黄居群中,淳安居群的遗传多样性水平最高,安吉居群的遗传多样性水平最低.Nei's基因多样性指数和Shannon信息指数均表明竹黄物种水平的遗传多样性高于居群水平. 相似文献
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以紫外(UV)与亚硝基胍诱变的竹黄菌(Shiraia bambusicola)菌株NU12和UV4为出发菌株,60℃处理5 min、紫外(距离30 cm,30 W)照射90s进行双亲原生质体灭活,通过30%聚乙二醇PEG6000介导原生质体融合20 min.结合平板初筛和高效液相色谱( HPLC)分析进行复筛,通过3轮重组融合操作,筛选出6株高产竹红菌甲素的融合株.其中融合菌株FIII - 21的竹红菌甲素产量达到80.4 mg/L,比原始出发菌株提高了58.9%~167.1%,且遗传稳定,具有较高的医药及工业应用价值. 相似文献
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Antiangiogenic activity of 11,11'-dideoxyverticillin, a natural product isolated from the fungus Shiraia bambusicola 总被引:2,自引:0,他引:2
Chen Y Zhang YX Li MH Zhao WM Shi YH Miao ZH Zhang XW Lin LP Ding J 《Biochemical and biophysical research communications》2005,329(4):1334-1342
The fungus Shiraia bambusicola yields the phytochemical 11,11'-dideoxyverticillin, which has been shown to possess potent anticancer activity both in vitro and in vivo. In this study, we reveal that 11,11'-dideoxyverticillin has anti-angiogenic activities and explore the potential mechanisms for this effect. Treatment with 11,11'-dideoxyverticillin inhibited the proliferation of human umbilical vein endothelial cells (HUVECs) with IC(50) values of 0.17+/-0.05muM for VEGF-stimulated cells and 0.39+/-0.08muM for serum-stimulated cells. 11,11'-Dideoxyverticillin also antagonized the antiapoptotic effects of VEGF on serum-deprived HUVECs, inhibited VEGF-induced HUVEC migration in vitro, and blocked serum-induced HUVEC tube formation. Moreover, 11,11'-dideoxyverticillin completely blocked VEGF-induced microvessel sprouting from Matrigel-embedded rat aortic rings and vessel growth in Matrigel plugs in mice. In addition, 11,11'-dideoxyverticillin decreased VEGF secretion by MDA-MB-468 breast cancer cells, and significantly suppressed VEGF-induced tyrosine phosphorylation of Flt-1 and KDR/Flk-1. This inhibition of receptor phosphorylation was correlated with a marked decrease in VEGF-triggered pERK activation and a dramatic increase in pP38 MAPK, but no apparent change in pAkt. Together, these findings strongly suggest that 11,11'-dideoxyverticillin is a structurally novel angiogenesis inhibitor. 相似文献