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1.
Construction of an RFLP linkage map for cultivated sunflower 总被引:5,自引:0,他引:5
C. C. Jan B. A. Vick J. F. Miller A. L. Kahler E. T. Butler III. 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,96(1):15-22
An RFLP linkage map was constructed for cultivated sunflower Helianthus annuus L., based on 271 loci detected by 232 cDNA probes. Ninety-three F2 plants of a cross between inbred lines RHA 271 and HA 234 were used as the mapping population. These genetic markers plus
a fertility restoration gene, Rf
1, defined 20 linkage groups, covering 1164 cM of the sunflower genome. Of the 71 loci 202 had codominant genotypic segregation,
with the rest showing dominant segregation. Thirty-two of the 232 probes gave multiple locus segregation. There were 39 clusters
of tightly linked markers with 0 cM distance among loci. This map has an average marker-to-marker distance of 4.6 cM, with
11 markerless regions exceeding 20 cM.
Received: 17 June 1997 / Accepted: 19 June 1997 相似文献
2.
A molecular genetic map of cassava (Manihot esculenta Crantz) 总被引:12,自引:0,他引:12
M. Fregene F. Angel R. Gomez F. Rodriguez P. Chavarriaga W. Roca J. Tohme M. Bonierbale 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,95(3):431-441
A genetic linkage map of cassava has been constructed with 132 RFLPs, 30 RAPDs, 3 microsatellites, and 3 isoenzyme markers
segregating from the heterozygous female parent of an intraspecific cross. The F1 cross was made between ‘TMS 30572’ and ‘CM 2177-2’, elite cassava cultivars from Nigeria and Colombia, respectively. The
map consists of 20 linkage groups spanning 931.6 cM or an estimated 60% of the cassava genome. Average marker density is 1
per 7.9 cM. Since the mapping population is an F1 cross between heterozygous parents, with unique alleles segregating from either parent, a second map was constructed from
the segregation of 107 RFLPs, 50 RAPDs, 1 microsatellite, and 1 isoenzyme marker from the male parent. Comparison of intervals
in the male-and female-derived maps, bounded by markers heterozygous in both parents, revealed significantly less meiotic
recombination in the gametes of the female than in the male parent. Six pairs of duplicated loci were detected by low-copy
genomic and cDNA sequences used as probes. Efforts are underway to saturate the cassava map with additional markers, to join
the male- and female-derived maps, and to elucidate genome organization in cassava.
Received: 5 July 1996/Accepted: 22 November 1996 相似文献
3.
D. E. Harry B. Temesgen D. B. Neale 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(3):327-336
We report a strategy for developing codominant PCR-based genetic markers by using sequenced cDNA clones from loblolly pine
(Pinus taeda L.). These clones were previously used as probes for detecting restriction fragment length polymorphisms (RFLPs) to generate
linkage maps. After assessing the complexity of banding patterns from Southern blots, we selected clones representing relatively
simple gene families, and then determined nucleotide sequences for about 200 bp at each end of the cDNA inserts. Specific
PCR primers were designed to amplify samples of genomic DNA derived from two loblolly pine mapping populations. Polymorphisms
were detected after digesting the amplified DNA fragments with a battery of restriction endonucleases, and most polymorphisms
were inherited in a Mendelian fashion. These newly identified genetic markers are codominant and relatively simple to use.
By assaying DNA from individuals used to construct RFLP maps, we show that most of these markers map to the same position
as the RFLP loci detected using their corresponding cDNAs as probes, implying that these markers have been converted from
RFLP to PCR-based methods. These PCR-based markers will be useful for genome mapping and population genetics.
Received: 10 February 1998 / Accepted: 25 February 1998 相似文献
4.
B. S. Vivek P. W. Simon 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,99(1-2):58-64
A 109-point linkage map consisting of three phenotypic loci (P
1, Y
2, and Rs), six restriction fragment length polymorphisms (RFLPs), two random amplified polymorphic DNAs (RAPDs), 96 amplified fragment
length polymorphisms (AFLPs), and two selective amplification of microsatellite polymorphic loci (SAMPL) was constructed for
carrot (Daucus carota L. ssp. sativus; 2n=2x=18). The incidence of polymorphism was 36% for RFLP probes, 20% for RAPD primers, and 42% for AFLP primers. The overall incidence
of disturbed segregation was 18%. Linkage relationships at a LOD score of 4.0 and θ=0.25 indicated 11 linkage groups. The
total map length was 534.4 cM and the map was clearly unsaturated with markers spaced at 4.9 cM. AFLP P6B15 was 1.7 cM from
P
1, AFLP P1B34 was 2.2 cM from Y
2, and AFLP P3B30XA was 8.1 cM from Rs.
Received: 2 September 1998 / Accepted: 28 November 1998 相似文献
5.
A genetic linkage map of cowpea (Vigna unguiculata) developed from a cross between two inbred, domesticated lines 总被引:1,自引:0,他引:1
C. M. Menéndez A. E. Hall P. Gepts 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,95(8):1210-1217
We have constructed a genetic linkage map within the cultivated gene pool of cowpea (2n=2x=22) from an F8 recombinant inbred population (94 individuals) derived from a cross between the inbreds IT84S-2049 and 524B. These breeding
lines, developed in Nigeria and California, show contrasting reactions against several pests and diseases and differ in several
morphological traits. Parental lines were screened with 332 random RAPD decamers, 74 RFLP probes (bean, cowpea and mung bean
genomic DNA clones), and 17 AFLP primer combinations. RAPD primers were twice as efficient as AFLP primers and RFLP probes
in detecting polymorphisms in this cross. The map consists of 181 loci, comprising 133 RAPDs, 19 RFLPs, 25 AFLPs, three morphological/classical
markers, and a biochemical marker (dehydrin). These markers identified 12 linkage groups spanning 972 cM with an average distance
of 6.4 cM between markers. Linkage groups ranged from 3 to 257 cM in length and included from 2 to 41 markers, respectively.
A gene for earliness was mapped on linkage group 2. Seed weight showed a significant association with a RAPD marker on linkage
group 5. This map should facilitate the identification of markers that “tag” genes for pest and disease resistance and other
traits in the cultivated gene pool of cowpea.
Received: 16 September 1996 / Accepted: 25 April 1997 相似文献
6.
An RFLP linkage map of Upland cotton, Gossypium hirsutum L. 总被引:15,自引:0,他引:15
Zachary W. Shappley J. N. Jenkins William R. Meredith Jack C. McCarty Jr. 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(5-6):756-761
Ninety-six F2.F3 bulked sampled plots of Upland cotton, Gossypium hirsutum L., from the cross of HS46×MARCABUCAG8US-1-88, were analyzed with 129 probe/enzyme combinations resulting in 138 RFLP loci.
Of the 84 loci that segregated as co-dominant, 76 of these fit a normal 1 : 2 : 1 ratio (non-significant chi square at P=0.05). Of the 54 loci that segregated as dominant genotypes, 50 of these fit a normal 3: 1 ratio (non-significant chi square
at P=0.05). These 138 loci were analyzed with the MAPMAKER∖ EXP program to determine linkage relationships among them. There were
120 loci arranged into 31 linkage groups. These covered 865 cM, or an estimated 18.6% of the cotton genome. The linkage groups
ranged from two to ten loci each and ranged in size from 0.5 to 107 cM. Eighteen loci were not linked.
Received: 31 March 1998 / Accepted: 29 April 1998 相似文献
7.
N. Nitta M. L. Farman S. A. Leong 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,95(1-2):20-32
A high-density genetic map of the rice blast fungus Magnaporthe grisea (Guy11×2539) was constructed by adding 87 cosmid-derived RFLP markers to previously generated maps. The new map consists
of 203 markers representing 132 independently segregating loci and spans approximately 900 cM with an average resolution of
4.5 cM. Mapping of 33 cosmid probes from the genetic map generated by Sweigard et al. has allowed the integration of two M. grisea maps. The integrated map showed that the linear order of markers along all seven chromosomes in both maps is in good agreement.
Thirty of eighty seven markers were derived from cosmid clones that contained the retrotransposon MAGGY (M. grisea gypsy element). Mapping of single-copy DNA sequences associated with the MAGGY cosmids indicated that MAGGY elements are
scattered throughout the fungal genome. In eight cases, the probes associated with MAGGY elements showed abnormal segregation
patterns. This suggests that MAGGY may be involved in genomic rearrangements. Two RFLP probes linked to MAGGY elements, and
another flanking other repetitive DNA elements, identified sequences that were duplicated in the Guy11 genome. Most of the
MAGGY cosmids also contained other classes of repetitive DNA suggesting that repetitive DNA sequences tend to cluster in the
M. grisea genome.
Received: 17 February 1997 / Accepted: 21 February 1997 相似文献
8.
R. N. Morgan J. P. Wilson W. W. Hanna P. Ozias-Akins 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,96(3-4):413-420
Pearl millet [Pennisetum glaucum (L.) R.Br.] is a warm-season grass used for food, feed, fodder and forage, primarily in countries of Africa and India but
grown around the world. The two most-destructive diseases to pearl millet in the United States are rust (caused by Puccinia substriata var. indica) and pyricularia leaf spot (caused by Pyricularia grisea). Genes for disease resistance to both pathogens have been transferred into agronomically acceptable forage and grain cultivars.
A study was undertaken to identify molecular markers for three rust loci and one pyricularia resistance locus. Three segregating
populations were screened for RAPDs using random decamer primers and for RFLPs using a core set of probes detecting single-copy
markers on the pearl millet map. The rust resistance gene Rr
1
from the pearl millet subspecies P. glaucum ssp. monodii was linked 8.5 cM from the RAPD OP-G8350. The linkage of two RFLP markers, Xpsm108 (15.5 cM) and Xpsm174 (17.7 cM), placed the Rr
1
gene on linkage-group 3 of the pearl millet map. Rust resistance genes from both Tift 89D2 and ICMP 83506 were placed on linkage-group 4 by determining genetic linkage to the RFLP marker Xpsm716 (4.9 and 0.0 cM, respectively). Resistance in ICMP 83506 was also linked to the RFLP marker Xpsm306 (10.0 cM), while resistance in Tift 89D2 was linked to RAPD markers OP-K19350 (8.8 cM) and OP-O8350 (19.6 cM). Fragments from OP-K19 and OP-O8 in the ICMP 83506 population, and Xpsm306 in the Tift 89D2 population, were monomorphic. Only one RAPD marker (OP-D11700, 5.6 cM) was linked to pyricularia leaf spot resistance. Attempts to detect polymorphisms with rice RFLP probes linked to
rice blast resistance (Pyricularia oryzae; syn=P. grisea) were unsuccessful.
Received: 19 May 1997 / Accepted: 21 October 1997 相似文献
9.
S. C. K. Milach H. W. Rines R. L. Phillips 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,95(5-6):783-790
Restriction fragment length polymorphism (RFLP) analysis provides a valuable tool for characterizing and understanding relationships
among genes for useful traits in crop species, particularly in ones with complex genomes such as the hexaploid cultivated
oat Avena sativa L. (2n=6x=42). Using Bulked Segregant Analysis (BSA) and F2 RFLP linkage data, we mapped three dominant oat dwarfing loci to different regions of the oat genome. Dw6, in oat line OT207, is 3.3±1.3 cM from the Xumn145B locus, which has not been placed on the hexaploid oat linkage map. Dw7, in line NC2469-3, is 4.3±2.3 cM from Xcdo1437B and 33±4.1 cM from Xcdo708B. This places Dw7 to linkage group 22. Dw8, in the Japanese lines AV17/3/10 and AV18/2/4, mapped 4.9±2.2 cM from Xcdo1319A in an AV17/3/10בKanota’ F2 population and 6.6±2.6 cM from it in an AV18/2/4בKanota’ population. This places Dw8 to linkage group 3. Aneuploid analysis of markers linked to the dwarfing genes located Dw6 on the smallest oat chromosome (chromosome 18) and Dw7 on the longest satellited chromosome (chromosome 19). The RFLP markers closely linked to the three dwarfing genes identify
distinct regions of the oat genome that contribute to plant height and they should be useful in characterizing new genetic
sources of dwarfness in oat.
Received: 8 May 1997 / Accepted: 20 May 1997 相似文献
10.
W. Y. Cheung L. Friesen G. F. W. Rakow G. Séguin-Swartz B. S. Landry 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,94(6-7):841-851
A genetic linkage map of Brassica juncea was constructed based on restriction fragment length polymorphism (RFLP) detected by anonymous cDNA markers from B. napus, using a segregating F1-derived doubled haploid (DH) progeny from a cross between a canola-quality mustard line (J90-4317) and a high-oil-content
mustard line (J90-2733). The RFLP probes consisted of 229 cDNA probes from B. napus and a B. napus tandem repeat sequence, RDA2. The map consisted of 343 marker loci arranged in 18 major linkage groups plus five small segments
with two to five marker loci, covering a total map distance of 2073 cM. Twenty-four percent of the markers were dominant in
nature. Sixty-two percent of the marker loci were duplicated, and the majority were involved in inter-linkage group duplications,
illustrating that complex duplications and subsequent rearrangements occurred after allopolyploidy. Deviation from the Mendelian
segregation ratio for a DH population was observed for 27% of the markers. Two-thirds of these markers with a skewed segregation
were clustered in 6 linkage groups and two unassigned segments. The overall average marker interval of the B. juncea map reported here was 6.6 cM, which would provide a marker density satisfactory for efficient use of the map in breeding
applications, such as tagging of important agronomic traits and marker-assisted selection.
Received: 14 May 1996 / Accepted: 11 October 1996 相似文献