显示弹性,胶原纤维的双重组合染色法

在特殊染色中,分别显示胶原纤维和弹性纤维的方法较多,但是这二种染色效果较差,多年来为了使动物和人体组织中能同时可靠的显示胶原和弹性纤维成分,并在较长时间内不褪色。为此我们进行了反复的实验性研究,在选用多种染料中,发现酸性染料 Ponceau S(丽春红 S),碱性染料Victoria blue(维多利亚蓝)等试剂,经过重新组合后,能更好的显示胶原纤维呈红色,弹性纤维呈蓝绿色,肌肉等呈黄色,染色色彩鲜艳,对比清晰。其方法简单,容易掌握,这是一种较为理想的双重组合染色方法。     

丽春红2R—亮绿染色法在显示神经髓鞘中的应用

任何因素所致的神经损伤 ,均可导致髓鞘的变性、髓鞘的脱失程度。常规髓鞘染色多采用传统的苏木素及锇酸染色法。苏木素法染色时间过长 (约 2 4h) ,染色步骤复杂 ,温度需控制在 5 0～ 5 5℃ ,分化时由于分化液作用速度较快 ,不便于掌握 ,易造成染色失败 ;而锇酸价格昂贵 ,浸透性差。这样将使神经组织的病理诊断及研究受到很大影响。为此 ,我们根据神经髓鞘的组织结构特点及染色原理 ,采用丽春红 2 R-亮绿组织化学染色方法 ,收到了满意的效果。材料和方法1.标本来源 :活检、尸检及动物正常或病变的脑组织、脊髓和周围神经组织 ,均经福尔马林…     

SR-G-AB显示胶原、网状纤维和粘液的复合染色法

在组织细胞的染色中,为了证明双重纤维和粘液物质,通过分别选用和组合的天狼星红(Sirius)苦味酸、Comori和阿尔辛蓝(Alcian blue)醋酸染色试剂,已能够显示鼠肺组织中胶原纤维呈红色,网状纤维呈黑色,粘液物质呈绿蓝色,背景呈黄色。对比清晰,色彩鲜艳,是较为理想的复合染色方法。     

实验动物组织血管铁质反应与胶原、弹性纤维和肌纤维的组合染色法

在进行实验动物组织染色反应中,为了证明血管铁质物质与胶原、弹性纤维和肌纤维的分布情况,选用丽春红S苦味酸(Ponceau S picric acid)、间苯二酚碱性复红(Resorein basic fuchsin)与Perls铁法(简称P-PA-R-BF-P法),对大鼠组织进行组合染色,能够较好显示组织血管中铁质物质与胶原、弹性纤维和肌纤维,铁质物质呈蓝色,胶原纤维呈红色,弹性纤维呈棕色,肌纤维呈黄色。这是一种相互对比清晰的组合染色方法。     

神经髓鞘染色新方法的建立及其原理探讨

在神经病理诊断和研究工作中,髓鞘染色是一种常用的方法。任何因素的神经纤维损伤,均可导致髓鞘的变性、崩解或脱失。普通染色中髓鞘不易着色,在正常或病理情况下均需要特殊染色法来观察髓鞘的损害程度。常规髓鞘特殊染色多采用传统的苏木素（如Wei1氏和Loyez氏法）及锇酸染色法。     

一种新的显示髓鞘和神经纤维的双重染色方法

一种新的显示髓鞘和神经纤维的双重染色方法邢惠清,田玉旺,丁华野,邓永江,黄晓南（北京军区总医院病理科,北京１００７００）区别髓鞘是否完整和坏变,常规采用Ｗｉｌｌ氏染色法,此法要求：切片厚度１５—２０μｍ;染色温度５６—６０°及时间要求甚严;褪色难以掌...     

变色素2R水溶液显示神经髓鞘的新方法

本文应用变色素 2R(Chromotrope 2R)显示神经髓鞘的新方法,将神经髓鞘染成红色,形态清晰,颜色鲜艳,操作简便,易于掌握,是显示神经髓鞘较为优良的方法,为神经组织的病理诊断和研究提供了新的技术方法.标本来源:尸检脑组织、脊髓和周围神经组织,经10%甲醛固定,石蜡包埋,切片5μm.试剂的配制:①变色素2R染液:变色素2R(北京化工厂)0.5g,磷钨酸0.6g,冰醋酸1.0g,蒸馏水加至100ml.放置4℃冰箱长期保存,用前回温.②0.2%冰醋酸水溶液:冰醋酸0.2ml,蒸馏水加到100ml.染色步骤:     

结晶紫染色法显示弹力纤维的应用

病理诊断常用 Comori醛复红 ,Weigert来复红 ,Verhochff法 Unna地衣红对弹力纤维[1] 进行特殊染色来观察显示皮肤、肺、血管组织中弹力纤维的增生、变性、散乱、断裂 ,其染色结果可呈紫色、兰黑色、褐色 ,但复染苏木素定位胞核和 VG显示胶原纤维染色后 ,对比不鲜明 ,经尝试用结晶紫改良 Weigert染色法后 ,弹力纤维呈绿色 ,胶原纤维红色 ,肌肉组织及背景黄色 ,对比鲜明 ,色彩艳丽、清晰 ,收到了理想效果。材料和方法1 .材料1 .1　标本来源 :本室活检和尸检正常或病变的心肌、皮肤、肺组织 ,经福尔马林固定 ,常规石蜡切片 5 μm。1 .2　染…     

介绍一种新的神经髓鞘染色法

介绍一种新的神经髓鞘染色法田玉旺,丁华野,邢惠清,黄晓南（北京军区总医院病理科北京１００７００）在神经病理的诊断和研究工作中,髓鞘染色是一种常规的染色方法。古典的染色方法具有下列不足之处：染色时间过长,结果欠佳,步骤复杂,分化难以掌握,且易造成染色失...     

神经细胞尼氏体染色方法改良

组织细胞的染色无论在病理学诊断还是在科学研究与教学工作中,都具有重要的意义和使用价值。为了更好的研究神经组织,方便医学诊断、科研和教学工作,对3种常见尼氏体染色方法包括硫堇、甲苯胺蓝、吉姆萨染色法做了一些改良。改进的尼氏体染色法能够使脑组织切片更清晰美观,更有利于科研工作者对神经组织及尼氏体的研究。     

Demonstration of Lipofuscin and Nissl Bodies in Crystal Violet Stained Sections Using a Fluorescence Technique or Pyronin Y Stain

This paper presents two simple, reliable methods for identification of lipofuscin and Nissl bodies in the same section. One method shows that lipofuscin stained with crystal violet retains its ability to fluoresce and can be observed under the fluorescence microscope after the stain has faded. Fading is accompanied by a gradual increase in the intensity of the fluorescence and is complete in about 5 min. Exciting illumination from this part of the spectrum also substantially fades staining of other autofluorescing tissue elements, such as lipids. Nonfluorescing structures, such as Nissl bodies, remain stained. By changing from transillumination with tungsten light to epifluorescent illumination and vice versa, both types of structures—Nissl bodies and lipofuscin—can be identified in the same section. The second technique uses pyronin Y for staining Nissl bodies in preparations previously stained with crystal violet. Nissl bodies are stained pink but lipofuscin remains violet. Lipofuscin in these sections also remains autofluorescent after the crystal violet stain has faded under violet or near-UV light.     

Demonstration of lipofuscin and Nissl bodies in crystal violet stained sections using a fluorescence technique or pyronin Y stain

This paper presents two simple, reliable methods for identification of lipofuscin and Nissl bodies in the same section. One method shows that lipofuscin stained with crystal violet retains its ability to fluoresce and can be observed under the fluorescence microscope after the stain has faded. Fading is accompanied by a gradual increase in the intensity of the fluorescence and is complete in about 5 min. Exciting illumination from this part of the spectrum also substantially fades staining of other autofluorescing tissue elements, such as lipids. Nonfluorescing structures, such as Nissl bodies, remain stained. By changing from transillumination with tungsten light to epifluorescent illumination and vice versa, both types of structures--Nissl bodies and lipofuscin--can be identified in the same section. The second technique uses pyronin Y for staining Nissl bodies in preparations previously stained with crystal violet. Nissl bodies are stained pink but lipofuscin remains violet. Lipofuscin in these sections also remains autofluorescent after the crystal violet stain has faded under violet or near-UV light.     

Short Nissl Staining for Incubated Cryostat Sections of the Brain

Nissl stain often binds poorly to cryostat sections which have been incubated in solutions of radiolabeled ligands. Such incubation is used in receptor autoradiography of the brain when using the in vitro method. We have developed a rapid (16 min) modification of Nissl staining for sections that bind stain poroly, e.g., incubated sections. The method stains well sections which cannot be stained with other rapid Nissl staining methods.     

Short Nissl Staining for Incubated Cryostat Sections of the Brain

Nissl stain often binds poorly to cryostat sections which have been incubated in solutions of radiolabeled ligands. Such incubation is used in receptor autoradiography of the brain when using the in vitro method. We have developed a rapid (16 min) modification of Nissl staining for sections that bind stain poroly, e.g., incubated sections. The method stains well sections which cannot be stained with other rapid Nissl staining methods.     

Laser-Capture Microdissection Impairs Activity-Based Protein Profiles for Serine Hydrolase in Human Lung Adenocarcinoma

Laser-capture microdissection (LCM) enables the selection of a specific and pure cell population from a heterogenous tissue such as tumors. Activity-based protein profiling/profile (ABPP) is a chemical technology using enzyme-specific active site-directed probes to read out the functional state of many enzymes directly in any proteome. The aim of this work was to assess the compatibility of LCM with downstream ABPP for serine hydrolase (SH) in human lung adenocarcinoma. Fresh frozen lung adenocarcinoma tissue was stained with hematoxylin, toluidine blue, or methyl green (MG). Proteome from stained tissue was labeled further with SH-directed probes, and ABPPs were determined on a one-dimensional gel-based approach. This allowed us to assess the impact of staining procedures on their ABPPs. The effect of the LCM process on ABPPs was assessed furthermore using MG-stained lung adenocarcinoma tissue. The staining procedures led to strong changes in ABPPs. However, MG staining seemed the most compatible with downstream ABPP. MG-stained, laser-captured, microdissected tissue showed additional change in profiles as a result of the denaturing property of extraction buffer but not to the microdissection process itself. LCM staining procedures but not microdissection per se interfered with downstream ABPP and led to a strong change in ABPPs of SHs in human lung adenocarcinoma.     

Safranin O Staining Using a Microwave Oven

We investigated the effects of microwave irradiation on a safranin O staining method for paraffin sections of formalin fixed rabbit larynx. The control sections were stained according to the conventional method, and the experimental sections were stained in microwave oven for 10 sec at 360 W in Weigert's iron hematoxylin, and for 30 sec at 360 W in fast green and 0.1% safranin O staining solutions. Light microscopic examination of the sections revealed that the microwave heating did not adversely affect the staining properties of cartilage tissue compared to the conventional staining method. Small differences such as darker staining of the matrix and shrinkage of the cytoplasm was observed in some microwave treated sections. The present study revealed that microwave application can be used safely for the safranin O method with the advantage of reduced staining time.     

Safranin O Staining Using a Microwave Oven

We investigated the effects of microwave irradiation on a safranin O staining method for paraffin sections of formalin fixed rabbit larynx. The control sections were stained according to the conventional method, and the experimental sections were stained in microwave oven for 10 sec at 360 W in Weigert's iron hematoxylin, and for 30 sec at 360 W in fast green and 0.1% safranin O staining solutions. Light microscopic examination of the sections revealed that the microwave heating did not adversely affect the staining properties of cartilage tissue compared to the conventional staining method. Small differences such as darker staining of the matrix and shrinkage of the cytoplasm was observed in some microwave treated sections. The present study revealed that microwave application can be used safely for the safranin O method with the advantage of reduced staining time.     

Mucopolysaccharides in the dog spinal cord and dorsal root ganglia. A histochemical study.

A histochemical study of mucopolysaccharides in the dog spinal cord and dorsal root ganglia is reported in this paper. The histochemical techniques used were the following: PAS, colloidal iron, toluidine blue (pH 5.4 and 3.5), thionine (pH 5.4 and 3.5) and alcian blue 8GX (pH 1 and 2.5). Some histological stains were used also. Two types of neurons could be observed in spinal cord sections stained with colloidal iron techniques. In some neuron a border line of mucosubstances could be seen. In the dorsal root ganglia, different patterns of Nissl bodies distribution in neurons were described. This different distribution of Nissl bodies is associated with different metachromatic colorations of neurons. By using the colloidal iron method, two types of neurons were also revealed in the dorsal root ganglia: some neurons are of a yellow, small-sized and star-shaped type and others are of a light green, larger and round-shaped type. Mucosubstances in the endoneurium and perineurium of nerve fibers, in the Ranvier nodules and in the Schmidt-Lantermann incisures were observed. The possibility that the functional rhythm in some cases might be responsible for the difference in coloration between the dorsal root ganglia neurons is suggested.     

金胺O荧光染色在石蜡组织切片麻风病诊断中的应用

目的为了验证金胺O荧光染色法应用于石蜡组织切片麻风杆菌检测的可行性。方法用金胺O荧光法对6例确诊为麻风病的病理组织切片进行染色,并与抗酸染色结果进行对比。结果荧光染色法6例结果均为阳性,在暗背景下麻风杆菌显示明亮淡绿色荧光;在菌量较少荧光染色片中寻找单根麻风杆菌,较抗酸染色片更为容易。结论金胺O荧光染色法可用于石蜡组织切片麻风病的诊断,麻风杆菌单根散在时比抗酸染色法有一定优势。     

Differential Staining of Two Subpopulations of Purkinje Neurons in Rat Cerebellum with Acid Dyes

We present a new method that stains differently two subpopulations of Purkinje cells in the adult rat. Deparaffinized sections of cerebella, fixed by perfusion with buffered glutaraldehyde or Bouin's fluid were stained with 0.5% light green in 50% ethanolf 10-30 min). The excess dye was removed with saturated aqueous picric acid (10-30 min). At this point some Purkinje cells appeared as lightly stained neurons, while others were strongly stained. Slides were immersed in 0.5% aqueous acid fuchsin for approximately 1 min until the lightly stained neurons acquired a red color. Following immersion in 1% phosphotungstic acid, slides were rapidly dehydrated in ethanol, passed to xylene and mounted in Canada balsam. Two subpopulations of Purkinje cells differing in their protein content in somata and proximal dendrites stained differentially by this method. They occurred in all coronal and sagittal sections and in patches or stripes. Their relative proportion varied from lobule to lobule. A second staining method used potassium permanganate as the sole staining reagent. The staining reagent can be used on sections previously stained with the acid dyes. Purkinje cells appeared as subsets of brownish to deep brown stained neurons, the latter ones corresponding to green stained cells in the dichromic method. The results obtained indicated that the subpopulations reflect real differences among individual neurons and are not artifacts. The technique holds promise for identifying and localizing subsets of Purkinje cells differing in their protein content under normal and experimental conditions and for their further characterization by combined staining and histochemical procedures.