共查询到20条相似文献,搜索用时 31 毫秒
1.
Xu-De Wang Xian-En Zhang Yong-Chao Guo Zhi-Ping Zhang Zhu-An Cao Ya-Feng Zhou 《Biotechnology letters》2009,31(5):711-717
The gdh and gdhr genes, encoding B12-dependent glycerol dehydratase (GDH) and glycerol dehydratase reactivase (GDHR), respectively, in Klebsiella pneumoniae, were cloned and expressed in E. coli. Part of the β-subunit was lost during GDH purification when co-expressing α, β and γ subunit. This was overcome by fusing
the β-subunit to α- or γ-subunit with/without the insertion of a linker peptide between the fusion moieties. The kinetic properties
of the fusion enzymes were characterized and compared with wild type enzyme. The results demonstrated that the fusion protein
GDHALB/C, constructed by linking the N-terminal of β-subunit to the C-terminal of α subunit through a (Gly4Ser)4 linker peptide, had the greatest catalytic activity. Similar to the wild-type enzyme, GDHALB/C underwent mechanism-based
inactivation by glycerol during catalysis and could be reactivated by GDHR.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
2.
Evelyne Jouvin-Marche Mariza Goncalves Morgado Nikolaus Trede Patrice N. Marche Dominique Couez Isabelle Hue Carine Gris Marie Malissen Pierre-André Cazenave 《Immunogenetics》1989,30(2):99-104
Genomic DNA from a large panel of inbred strains of mice were hybridized sequentially with 15 Vα, 2 Vδ, 1 Cα, and 1 Cδ probes. Most of the Vα probes detected a high degree of plymorphism and have allowed the definition of five mouse T-cell receptor α (Tcr
α) haplotypes. One of these haplotypes (Tcr
α
e
) appears to arise from a recombination between theTcr
α
b
andTcr
α
a
haplotypes, the latter being the most frequently found in the conventional inbred strains. This recombination event clearly
indicates that the members of at least 11 Vα subfamilies are not closely linked but highly interspersed with one another on chromosome 14. 相似文献
3.
P. Bhattacharya 《Journal of biosciences》1999,24(4):441-444
Accumulation of immunoglobulin Ig RNA (from several loci viz., CH, Cα, Jk-Ck and Sμ during Igμ isotype switching) in B cells and T cell receptor (TCR) RNAs (α, β andγ) in T cells of unusual sizes emanating from germline and rearranged genes were reported to accumulate in human and mouse
(and murine too). The precise mechanism and function of these sterile RNA species are yet to be delineated. Similar accumulation
of RNA species of unusual sizes were identified with DNA-RNA hybridization and isolation of cDNA employing with DNA and antibody
probes in mouse hybridoma, murine tumour, non-human primate marmoset tumour and human leukemic cells. 相似文献
4.
Laurent Vidard Thierry Roger Ghislaine Pham Jacques Couderc Yolande Bouthillier Jean-Claude Mevel Denise Mouton Michel Seman 《Immunogenetics》1990,32(1):27-33
Tcrb andTcrg gene polymorphism was investigated in high (H) and low (L) responder Biozzi mice from selection I, II, and GS by Southern blot analysis with appropriateV andC probes. No polymorphism of theTcrb haplotype was detected between H and L mice in all selections which were all found to be of the BALB/c type. The H-I and H-II g genotype was of BALB/c and DBA/2 type, respectively. In contrast, a newTcrg haplotype shared by L-I and L-II mice was identified and characterized by C1, 2, 3, C4, V1, 2, 3, V5, and V6 restriction fragment length polymorphisms (RFLPs).Tcrg genotypes were not fixed in the GS selection and two additional new haplotypes were identified in two L-GS mice. An attempt was made to correlate the L-Ig genotype with the low responder status by analyzingg haplotypes among highest and lowest responder (H-1 x L-I)F2 hybrids immunized with sheep red blood cells (SRBC). No correlation was found in this segregation study, whereas a highly significant one was established with theH-2 haplotype, a locus already known to participate in the genetic control of H-I/L-I difference. The lack of correlation between SRBC response and theTcrg genotype was consistent with the heterogenousg haplotypes found in mice of the GS selection. Together, the present results suggest that H and L mice have the sameTcrab potential repertoire and that T-cell receptor (Tcr) genes cannot be considered as immune response genes in this model. Our results also indicate that the F2 segregation analysis, given a polymorphic gene, is suitable for an investigation of its immune response functions. 相似文献
5.
Tomomasa Watanabe Nobumoto Miyashita Masahiko Nishimura Naruya Saitou Yukimasa Hayashi Kazuo Moriwaki 《Biochemical genetics》1989,27(1-2):119-130
Restriction endonuclease fragment length variants in mice were compared by Southern blot analysis using the cDNA probe pcXP33 for the chymotrypsin gene. The variants were detected in the restriction patterns generated by fragments from digestions withBglII,EcoRI,HindIII,Pstl,SacI, andXbaI. The set of protein phenotypes and the restriction patterns of chymotrypsin gene were examined in many laboratory strains and wild subspecies. Most laboratory strains (26 strains) are grouped into a set defined as Set 1, but only a few laboratory strains (AU/SsJ and five BALB/c sublines) are classified as belonging to Set 2. Of wild subspecies, only BRV-MPL (M. brevirostris) can be placed in Set 1, while DOM-BLG and SK/Cam (M. domesticus) belong in Set 2. The assignment of an appropriate set defined by the characteristics of the chymotrypsin gene has also been investigated inM. musculus, two Chinese subspecies,M. yamashinai, M. molossinus, andM. castaneus, and the evolutionary relationship between laboratory mice and various subspecies ofMus has been examined. 相似文献
6.
By the use of the Immobiline technique at pH ranges 7.0–7.6 and 6.9–7.9, 16 different hemoglobin (Hb) phenotypes were observed
in 61 English Saanen goats. They are explained in this breed by a genetic theory of five β-globin genes (A
4,A
6,A
8,E, andD) and two closely linked α-globin loci (′α and ″α) of which the ″α has a variant allele, provisionally called ″α
X
. Family data together with observed and expected Hb frequencies were in agreement with the genetic theory. Among six Barbary
sheep there were three Hb phenotypes explained by the occurrence of the β-chain allelesB andC
na. 相似文献
7.
Yuka Sakai Shigeki Miyawaki Atsuko Shimizu Kyoko Ohno Tomomasa Watanabe 《Biochemical genetics》1991,29(1-2):103-113
Restriction endonuclease fragment length variations (RFLV) were detected in mice with DNA probes for myelin basic protein (Mbp), glucocorticoid receptor-1 (Grl-1), and Friend MuLV integration site-2 (Fim-2). RFLV of theMbp gene were found inSacI restriction patterns, RFLV of theGrl-1 gene were found inEcoRV patterns, and RFLV of theFim-2 were found inBglII patterns. A three-point backcross was carried out by the backcross mating (C57BL/KsJ-spm/spm × MOL-MIT)F1 males × C57BL/KsJ-spm/spm; spm is an autosomal recessive gene causing sphingomyelinosis. From the results,spm, Grl-1, Fim-2, andMbp loci were mapped on chromosome 18, and the following order of genes is proposed, with distances between genes in parentheses: centromere—spm—(7.8 cM)—Grl-1—(7.8 cM)—Fim-2—(39.1 cM)—Mbp—telomere. All laboratory strains and two European subspecies (Mus mus domesticus andM. m. brevirostris) carry theGrl-1
a
,Fim-2
a
, andMbp
a
alleles. In contrast, another wild subspecies from Europe (M. m. musculus) and some Asian subspecies (M. m. molossinus, Chinese mice of wild origin, andM. m. yamashinai) carry theGrl-1
b
,Fim-2
b
, andMbp
b
alleles. Onlycastaneus strains carry the intermediate combination of theGrl-1
b
,Fim-2
a
, andMbp
b
alleles. 相似文献
8.
Tomomasa Watanabe Kyoko Ohno Atsuko Shimizu Yuka Sakai Masahide Takahashi Naoki Takahashi 《Biochemical genetics》1990,28(5-6):257-266
Restriction endonuclease fragment length variations (RFLV) were detected by use of the cDNA probeHox-3.1 for the homeo box-3.1 gene and also thec-myc oncogene probe for exon 2. RFLV ofHox-3.1 were found inHindIII restriction patterns, and RFLV of theMyc-1.2 gene inEcoRV patterns. From the RFLV, theHox-3.1 andMyc-1.2 genes were mapped on chromosome 15. Three-point cross test data showed that the frequency of recombination is 26.4% betweenMyc-1.2 andGpt-1, 30.2% betweenGpt-1 andGdc-1, and 9.4% betweenGdc-1 andHox-3.1. The following order of these genes is proposed,Myc-1.2—Gpt-1—Gdc-1—Hox-3.1. All laboratory strains carry theHox-3.1
a andMyc-1.2
a alleles. Among strains of wild origin,domesticus strains carry only theHox-3.1
a andMyc-1.2
a alleles, as do the laboratory strains. One strain ofbrevirostris carries theHox-3.1
a andMyc-1.2
b alleles. Other wild subspecies from Europe and Asia,M. m. musculus, M. m. castaneus, M. m. molossinus, Chinese mice of wild origin, andM. m. yamashinai carry theHox-3.1
b andMyc-1.2
b alleles. 相似文献
9.
Daniela Herrmann James M. Sodetz Christian Rittner Peter M. Schneider 《Immunogenetics》1989,30(4):291-295
The eighth component of human complement consists of three subunits of different molecular mass, which are coded for by three
separate genetic loci. Polymorphisms have been described at the protein level for the alpha and beta subunits by means of
sodium dodecyl sulfate gel electrophoresis and isoelectric focusing. Using a full-length humanC8β cDNA probe, we have studied more than 100 individuals by Southern blot analysis to detect DNA polymorphisms. We have found
two restriction fragment length polymorphisms (RFLPs) with the enzymesTaqI andBam HI. TheTaq I polymorphism is defined by two alleles, i.e., a single 4.9 kb fragment or two 2.8/2.1 kb fragments. The allele frequencies
are 0.68 and 0.32, respectively. The second RFLP withBam HI is correlated with theTaq I variants: 3 kbBam HI; 4.9 kbTaq I and 3.3 kbBam HI; 2.8/2.1 kbTaqI. Both RFLPs could be mapped to the 3′ portion of theC8β gene. Based on the size of genomic restriction fragments, theC8β gene can be estimated to have a size of 32–36 kb. Because of the even frequency distribution, theC8β DNA polymorphisms may be useful in gene mapping and disease association studies. 相似文献
10.
Solaiman DK 《Journal of industrial microbiology & biotechnology》2003,30(5):322-326
Pseudomonas resinovorans harbors two isogenic poly(hydroxyalkanoates) (PHAs) synthase genes (phaC1
Pre
, phaC2
Pre
) responsible for the production of intracellular medium-chain-length (mcl-)PHAs. Sequence analysis showed that the putative
gene-products of these genes contain a conserved α/β-hydrolase fold in the carboxy-terminal half of the proteins. Hybrid genes pha7 and pha8 were constructed by exchanging the α/β-hydrolase-fold coding portions of phaC1
Pre
and phaC2
Pre
at the 3′ terminal. When grown with decanoate as carbon source, the pha7- or pha8-transformed Escherichia coli LS1298 produced PHAs containing 73–75% β-hydroxydecanoate (β-HD) and 25–27% β-hydroxyoctanoate (β-HO). Deletion mutants,
Δpha7 and Δpha8, were isolated during the PCR-based construction of pha7 and pha8, respectively. Cells harboring these mutants produced PHAs containing 55–60 mol% β-HD and 40–45 mol% β-HO. These results
demonstrate the feasibility of generating active hybrid mcl-PHA synthase genes and their mutants with the potential of producing
polymers having a varied repeat-unit composition. 相似文献
11.
Hoh JF Kim Y Lim JH Sieber LG Lucas CA Zhong WW 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》2007,177(2):153-163
Cardiac myosins and their subunit compositions were studied in ten species of marsupial mammals. Using native gel electrophoresis,
ventricular myosin in macropodoids showed three isoforms, V
1, V
2 and V
3, and western blots using specific anti-α- and anti-β-cardiac myosin heavy chain (MyHC) antibodies showed their MyHC compositions
to be αα, αβ and ββ, respectively. Atrial myosin showed αα MyHC composition but differed from V
1 in light chain composition. Small marsupials (Sminthopsis crassicaudata, Antechinus stuartii, Antechinus flavipes) showed virtually pure V
1, while the larger (1–3 kg) Pseudocheirus peregrinus and Trichosurus vulpecula showed virtually pure V
3. The five macropodoids (Bettongia penicillata, Macropus eugenii, Wallabia bicolour, M. rufus and M.
giganteus), ranging in body mass from 2 to 66 kg, expressed considerably more α-MyHC (22.8%) than expected for their body size. These
results show that cardiac myosins in marsupial mammals are substantially the same as their eutherian counterparts in subunit
composition and in the correlation of their expression with body size, the latter feature underlies the scaling of resting
heart rate and cardiac cross-bridge kinetics with specific metabolic rate. The data from macropodoids further suggest that
expression of cardiac myosins in mammals may also be influenced by their metabolic scope. 相似文献
12.
Andreas Lössl N. Adler R. Horn U. Frei G. Wenzel 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,99(1-2):1-10
One hundred and eighty dihaploid clones used for protoplast fusions, and 144 tetraploid German potato cultivars were analysed
for their cytoplasms using 11 homologous mt DNA-probes, and were classified as mitochondrial (mt) types α, β, γ, δ, and ɛ
according to their RFLP patterns. From the 4x cultivars, 79 had the typical mt-type β of Solanum tuberosum being different from the 46 cvs which had the mt-α type and 19 others with mt-γ. A dendrogram shows their relationships to
other Solanum species. The distantly related mt-ɛ was only found in di-haploids, and particularly in clones deriving from Solanum phureja and Solanum andigena. Accessory mt types will be actualized on website (http://www.edv.agrar.tu-muenchen.de/pbpz/ mm/mt/al1.htm). In order to
evaluate the genetic potential of novel plastid-mitochondrial configurations we have analyzed four representative populations,
which derive from different fusion-combination classes: [α (+) β], [α (+) γ], [α (+) δ] and [α (+) ɛ]. On the mitochondrial
expression level, hybrids from an [α (+) ɛ] fusion could be distinguished by in-organello translation from [α (+) β] hybrids, and other di-haploids, by an additional translation product of 15 kDa. In fusion parents
with mt-α and -γ an additional atp6 reading frame is detectable in sub-stoichiometric amounts by the use of specific PCR primers. The gene differs from the original
211 bp 3′ from the stop codon. Novel RFLP-patterns in 10% of the somatic hybrids were due to a high-rate replication of this
pre-existing parental genome region. A second characteristic for somatic hybrids was the partial addition of parental mt sub-genomes.
The major part of them revealed a new organization in their mt genomes at the mt-type characteristic loci rpl5, rps14, cob, rps10, coxI and rpl2, which contain recombination-specific repeats homologous to Petunia spp. and Nicotiana. A schematic model for the formation of novel mitochondrial genomes in potato somatic hybrids is provided.
Received: 7 November 1998 / Accepted: 30 November 1998 相似文献
13.
Sandoval A Arikkath J Monjaraz E Campbell KP Felix R 《Cellular and molecular neurobiology》2007,27(7):901-908
(1) Voltage-gated Ca2+ (CaV) channels are multi-subunit membrane complexes that allow depolarization-induced Ca2+ influx into cells. The skeletal muscle L-type CaV channels consist of an ion-conducting CaV1.1 subunit and auxiliary α2δ−1, β1 and γ1 subunits. This complex serves both as a CaV channel and as a voltage sensor for excitation–contraction coupling. (2) Though much is known about the mechanisms by which
the α2δ−1 and β1 subunits regulate CaV channel function, there is far less information on the γ1 subunit. Previously, we characterized the interaction of γ1 with the other components of the skeletal CaV channel complex, and showed that heterologous expression of this auxiliary subunit decreases Ca2+ current density in myotubes from γ1 null mice. (3) In the current report, using Western blotting we show that the expression of the CaV1.1 protein is significantly lower when it is heterologously co-expressed with γ1. Consistent with this, patch-clamp recordings showed that transient transfection of γ1 drastically inhibited macroscopic currents through recombinant N-type (CaV2.2/α2δ−1/β3) channels expressed in HEK-293 cells. (4) These findings provide evidence that co-expression of the auxiliary γ1 subunit results in a decreased expression of the ion-conducting subunit, which may help to explain the reduction in Ca2+ current density following γ1 transfection. 相似文献
14.
Relationship between tolerance and accumulation characteristics of cadmium in higher plants 总被引:3,自引:0,他引:3
Ten plant species belonging to 5 families,i.e., Cruciferae, Cucurbitaceae, Gramineae, Leguminosae, and Solanaceae, were grown in a sand soil at two pH levels. The soil
was subjected to an application of CdCl2 at rates of 0 to 700 mgCd·kg−1 soil. The relationship between Cd concentration in the shoots (tc) and soil (sc; NH4NO3 extractable) was expressed by the equation: log (tc)=α+βlog (sc). The coefficients α and β were estimated for each species at each level of soil pH. Plottings of the scores on α and β axes
showed that the Cd accumulation characteristics in the plants appeared to depend on the families irrespective of soil pH.
Based on theupper critical concentration of Cd in the tops (Ct), the Cruciferae and Leguminosae species were found to be the most and the least tolerant to Cd, respectively. The Ct values correlated exceedingly well with the values of α. 相似文献
15.
GABAA receptors composed of α, β and γ subunits display a significantly higher single-channel conductance than receptors comprised
of only α and β subunits. The pore of GABAA receptors is lined by the second transmembrane region from each of its five subunits and includes conserved threonines at
the 6′, 10′ and 13′ positions. At the 2′ position, however, a polar residue is present in the γ subunit but not the α or β
subunits. As residues at the 2′, 6′ and 10′ positions are exposed in the open channel and as such polar channel-lining residues
may interact with permeant ions by substituting for water interactions, we compared both the single-channel conductance and
the kinetic properties of wild-type α1β1 and α1β1γ2S receptors with two mutant receptors, αβγ(S2′A) and αβγ(S2′V). We found
that the single-channel conductance of both mutant αβγ receptors was significantly decreased with respect to wild-type αβγ,
with the presence of the larger valine side chain having the greatest effect. However, the conductance of the mutant αβγ receptors
remained larger than wild-type αβ channels. This reduction in the conductance of mutant αβγ receptors was observed at depolarized
potentials only (ECl = −1.8 mV), which revealed an asymmetry in the ion conduction pathway mediated by the γ2′ residue. The substitutions at the
γ2′ serine residue also altered the gating properties of the channel in addition to the effects on the conductance with the
open probability of the mutant channels being decreased while the mean open time increased. The data presented in this study
show that residues at the 2′ position in M2 of the γ subunit affects both single-channel conductance and receptor kinetics. 相似文献
16.
Tomomasa Watanabe Shigeo Masaki Naoki Takahashi Masahiko Nishimura Hideki Kato 《Biochemical genetics》1988,26(11-12):805-813
Restriction endonuclease fragment length polymorphisms (RFLPs) were found using the cDNA probe Hox-2.1 for the homeo box-2.1 gene in the mouse. Polymorphism was detected in restriction patterns generated by fragments fromHindIII digestion. The great majority of laboratory strains of mice carries theHox-2.1 a allele. Only two laboratory strains carry theHox-2.1 b allele. Among strains of wild origin, the European subspecies (Mus m. domesticus, M. m. brevirostris, andM. m. musculus) and some Asian subspecies (M. m. castaneus) carry theHox-2.1 a allele. The subspecies from Far Eastern countries (M. m. molossinus, Chinese mice of wild origin, andM. m. yamashinai) carry theHox-2.1 ballele. Using the RFLP, theHox-2.1 gene was mapped on chromosome 11. Three-point cross test data showed that the recombination frequency is 29.6% between theHba and theHox-2.1 genes and 23.5% between theHox-2.1 and theEs-3 genes. The gene order ofHba-Hox-2.1-Es-3 has been confirmed. 相似文献
17.
Hoh JF Withers KW Zhong WW 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》2008,178(3):279-284
The effect of drug-induced hypothyroidism on ventricular myosin gene expression was explored in a small marsupial, Antechinus flavipes. Pyrophosphate gel electrophoresis, SDS-PAGE and western blotting were used to analyse changes in native myosin isoforms
and myosin heavy chains (MyHCs) in response to hypothyroidism. In some animals, five instead of the normal three native myosin
components were found: V1a, V1b,V1c, V2 and V3, in order of decreasing mobility. In western blots, V1a, V1b, and V1c reacted with anti-α-MyHC antibody, but not with anti-β-MyHC, whereas V2 and V3 reacted with anti-β-MyHC antibody. SDS-PAGE of the unusual ventricular myosins revealed three MyHC isoforms, two of which
bound anti-α-MyHC antibody while the third bound anti-β-MyHC antibody. We conclude that V1a, V1b, V1c are triplets arising from the dimerization of two distinct α-MyHC isoforms. Hypothyroidism, verified by metabolic studies,
decreased α-MyHC content significantly (t-test, P < 0.001) from 91.6 ± 5.9% (SEM, n = 4) in control animals to 67.2 ± 5.7% (SEM, n = 4) in hypothyroid animals, with a concomitant increase in β-MyHC content. We conclude that in adult marsupials, ventricular
myosins are also responsive to changes in the thyroid state as found in eutherians, and suggest that evolution of the molecular
mechanisms underlying this thyroid responsiveness predate the divergence of marsupials and eutherians. 相似文献
18.
Tomofumi Okuno Shin Ji Motobayashi Hitoshi Ueno Katsuhiko Nakamuro 《Biological trace element research》2005,106(1):77-93
The objective of this study was to purify and characterize a mouse hepatic enzyme that directly generates CH3SeH from seleno-l-methionine (l-SeMet) by the α,γ-elimination reaction. The l-SeMet α,γ-elimination enzyme was ubiquitous in tissues from ICR mice and the activity was relatively high in the large intestine,
brain, and muscle, as well as the liver. Aging and sex of the mice did not have any significant influence on the activity
in the liver. The enzyme was purified from the mouse liver by ammonium sulfate precipitation and four kinds of column chromatography.
These procedures yielded a homogeneous enzyme, which was purified approx 1000-fold relative to mouse liver extract. Overall
recovery was approx 8%. The purified enzyme had a molecular mass of approx 160 kDa with four identical subunits. The K
m
value of the enzyme for the catalysis of l-SeMet was 15.5 m M, and the V
max was 0.29 units/mg protein. Pyridoxal 5′-phosphate (pyridoxal-P) was required as a cofactor because the holoenzyme could be
resolved to the apoenzyme by incubation with hydroxylamine and reconstituted by addition of pyridoxal-P. The enzyme showed
the optimum activity at around pH 8.0 and the highest activity at 50°C; it catalyzed the α,γ-elimination reactions of several
analogs such as d,l-homocysteine and l-homoserine in addition to l-SeMet. This enzyme also catalyzed the α,β-elimination reaction of Se-methylseleno-l-cysteine. However, l-methionine was inerts. Therefore, the purified enzyme was different from the bacterial l-methionine γ-lyase that metabolizes l-SeMet to CH3SeH, in terms of the substrate specificity. These results were the first identification of a mammalian enzyme that specifically
catalyzes the α,γ-elimination reaction of l-SeMet and immediately converts it to CH3SeH, an important metabolite of Se. 相似文献
19.
Asako Ando Jun Kawai Masahiro Maeda Kimiyoshi Tsuji John Trowsdale Hidetoshi Inoko 《Immunogenetics》1989,30(4):243-249
A genomic clone specifying a new HLA class II antigen β chain,DQB3, was isolated from a human genomic phage library using aDQB1 cDNA probe under low stringency conditions. Southern hybridization and nucleotide sequence analyses identified the β2 domain
exon (exon 3) with several deleterious mutations and the CP-TM-CY exon [connecting peptide, transmembrane, and cytoplasmic
regions, (exon 4)], but the first, second, and fifth exons encoding the 5′ UT-leader, the β1 domain, and the 3′ UT domain
of normal β chains, respectively, were entirely missing. The nucleotide sequences of these two exons were distinct from those
of other class II β chain genes, but slightly more related to theDQB1 andDQB2 genes than to other class II genes. TheDQB3 sequence mapped betweenDQA2 andDQB1, 15 kb upstream fromDQA2, by analysis of overlapping cosmid clones. This mapping was supported by the fact thatTaq I,Msp I, andBam HIDQB3 polymorphisms were perfectly correlated with theDQA2 polymorphism and not with any polymorphisms in theDR orDQ subregion, suggesting the presence of a hot spot for recombination betweenDQB3 andDQB1.
The nucleotide sequence data reported in this paper have been submitted to the GenBank nucleotide sequence database and have
been assigned the accession number M26577. 相似文献
20.
Sluan D. Lin Phil Cooper Jingly Fung Heinz-Ulrich G. Weier Edward M. Rubin 《Mammalian genome》2000,11(11):1024-1029
Genetic factors affecting postnatal γ-globin expression—a major modifier of the severity of both β-thalassemia and sickle
cell anemia—have been difficult to study. This is especially so in mice, an organism lacking a globin gene with an expression
pattern equivalent to that of human γ-globin. To model the human β-cluster in mice, with the goal of screening for loci affecting
human γ-globin expression in vivo, we introduced a human β-globin cluster YAC transgene into the genome of FVB/N mice. The
β-cluster contained a Greek hereditary persistence of fetal hemoglobin (HPFH) γ allele, resulting in postnatal expression
of human γ-globin in transgenic mice. The level of human γ-globin for various F1 hybrids derived from crosses between the FVB/N transgenics and other inbred mouse strains was assessed. The γ-globin level
of the (C3HeB/FeJ × FVB/N)F1 transgenic mice was noted to be significantly elevated. To map genes affecting postnatal γ-globin expression, we performed
a 20-centiMorgan (cM) genome scan of a (C3HeB/FeJ × FVB/N)F1 transgenics × FVB/N backcross, followed by high-resolution marker analysis of promising loci. From this analysis we mapped
a locus within an 18-cM interval of mouse Chromosome (Chr) 1 (LOD = 4.3) that contributes 10.9% of variation in γ-globin level.
Combining transgenic modeling of the human β-globin gene cluster with quantitative trait analysis, we have identified and
mapped a murine locus that impacts on human γ-globin level in vivo.
Received: 26 January, 2000 / Accepted: 2 May 2000 相似文献