共查询到20条相似文献,搜索用时 593 毫秒
1.
2.
3.
4.
5.
6.
7.
Qianzheng Zhu Gulzar Wani Hany H. Arab Mohamed A. El-Mahdy Alo Ray Altaf A. Wani 《DNA Repair》2009,8(2):262-273
Restoration of functionally intact chromatin structure following DNA damage processing is crucial for maintaining genetic and epigenetic information in human cells. Here, we show the UV-induced uH2A foci formation in cells lacking XPC, DDB2, CSA or CSB, but not in cells lacking XPA, XPG or XPF indicating that uH2A incorporation relied on successful damage repair occurring through either GGR or TCR sub-pathway. In contrast, XPA, XPG or XPF were not required for formation of γH2AX foci in asynchronous cells. Notably, the H2A ubiquitin ligase Ring1B, a component of Polycomb repressor complex 1, did not localize at DNA damage sites. However, histone chaperone CAF-1 showed distinct localization to the damage sites. Knockdown of CAF-1 p60 abolished CAF-1 as well as uH2A foci formation. CAF-1 p150 was found to associate with NER factors TFIIH, RPA p70 and PCNA in chromatin. These data demonstrate that successful NER of genomic lesions and prompt CAF-1-mediated chromatin restoration link uH2A incorporation at the sites of damage repair within chromatin. 相似文献
8.
9.
10.
11.
12.
13.
14.
15.
16.
Effect of DNA lesions on transcription elongation 总被引:6,自引:0,他引:6
17.
18.
19.