共查询到20条相似文献,搜索用时 171 毫秒
1.
Hamid Babavalian Mohammad Ali Amoozegar Ahmad Ali Pourbabaee Mehrdad Moosazadeh Moghaddam Fatemeh Shakeri 《Microbiology》2013,82(4):466-474
Iran has many hypersaline environments, both the permanent and seasonal ones. One of the seasonal hypersaline lakes in the central desert zone is Aran-Bidgol Lake in which microbial diversity has not been characterized, thus the potential usage of this microbial community in biotechnology remained unknown. In this study, screening the halophilic hydrolytic enzyme-producing bacteria from different areas of this lake led to isolation of 61 gram-positive and 22 gram-negative moderately halophilic bacteria. These bacterial isolates were shown to produce a wide variety of hydrolytic enzymes including DNase, inulinase, amylase, lipase, pectinase, protease, chitinase, pullulanase, cellulase, and xylanase. The most common enzymes were DNase and inulinase in gram-positive bacteria, lipase in gram-negative bacteria, and pullulanase and cellulase in gram-positive cocci. Interestingly, combined hydrolytic activates were observed in some isolates. According to their phenotypic characteristics and comparative partial 16S rRNA sequence analysis, the moderately halophilic strains belonged to the genera Halobacillus, Thalassobacillus, Bacillus, Salinicoccus, Idiomarina, Salicola, and Halomonas. 相似文献
2.
M. Roayaie Ardakani A. Poshtkouhian M. A. Amoozegar H. Zolgharnein 《Indian journal of microbiology》2012,52(1):94-98
Extracellular hydrolytic enzymes such as amylases, proteases, lipases and DNases have quite diverse potential usages in different
areas such as food industry, biomedical sciences and chemical industries, also it would be of great importance to have available
enzymes showing optimal activities at different values of salt concentrations and temperature. Halophiles are the most likely
source of such enzymes, because not only their enzymes are salt-tolerant, but many are also thermotolerant. The purpose of
this study was isolation of hydrolytic extracellular enzyme producing halophilic bacteria from water and sediment of the Persian
Gulf. Isolated bacteria from water and sediment were inoculated in media with concentration of 0–20% NaCl to determine the
optimum salt concentration for growth, isolates were also inoculated in 4 types of solid medium containing substrates of 3
extracellular hydrolytic enzymes including amylase, Protease and Lipase, to determine the quantitative detection of enzyme
production, selected strains after more accurate physiological and biochemical studies were identified regarding phylogeny
and molecular characteristics using 16S rRNA technique. Isolated enzyme producing bacteria belong to Pseudoalteromonas genera. 相似文献
3.
I. Boyadzhieva I. Tomova N. Radchenkova M. Kambourova A. Poli E. Vasileva-Tonkova 《Microbiology》2018,87(4):519-528
Investigations on the microbial life in several coastal solar salterns have revealed the presence of novel organisms and synthesis of unusual molecules active in extreme conditions which might be useful in different biotechnological industries. Biodiversity of heterotrophic aerobic bacteria isolated from two salterns, Pomorie salterns and Burgas salterns located at Burgas Bay, Black Sea coast, Bulgaria, as well as ability of the isolates to synthesize biotechnologically valuable compounds were investigated. The results revealed high taxonomic and metabolic bacterial diversity—we isolated 20 morphologically different moderately halophilic and two halotolerant strains affiliated with 11 species from eight genera referred to the phyla Proteobacteria, Firmicutes, and Actinobacteria. Gram-negative bacteria belonged to the genera Halomonas, Chromohalobacter, Salinivibrio, Cobetia, and Nesiotobacter, and gram-positive strains were representatives of the genera Virgibacillus, Salinicoccus, and Brevibacterium. All isolates were found to be alkalitolerant, and 41% of them were psychrotolerant. The strains degraded nine of the tested 18 substrates; polygalacturonase, catalase, phytase, and lipase producers were predominant. This is the first reported detection of xanthan lyase, gellan lyase, arabinase, and phytase activities in halophilic bacteria. Nine of the strains belonging to five different genera were found to produce exopolysaccharides (EPS). The highest level of EPS was observed in Chromohalobacter canadensis strain 28. More than a half of the strains displayed antimicrobial activity against one to five test bacteria and yeasts. The present study is the first report on halophilic bacteria isolated from salterns at the Black Sea coast indicating that the investigated area is an untapped resource of halophilic bacteria with biotechnological potential. 相似文献
4.
5.
Diversity of moderately halophilic bacteria producing extracellular hydrolytic enzymes 总被引:8,自引:0,他引:8
AIMS: The aim of this study was to determine the diversity of moderately halophilic bacteria with hydrolase activities. METHODS AND RESULTS: Screening bacteria from different hypersaline environments in South Spain led to the isolation of a total of 122 moderately halophilic bacteria able to produce different hydrolases (amylases, DNases, lipases, proteases and pullulanases). These bacteria are able to grow optimally in media with 5-15% salts and in most cases up to 20-25% salts. In contrast to strains belonging to previously described species, that showed very little hydrolase activities, environmental isolates produced a great variety of hydrolases. These strains were identified as members of the genera: Salinivibrio (55 strains), Halomonas (25 strains), Chromohalobacter (two strains), Bacillus-Salibacillus (29 strains), Salinicoccus (two strains) and Marinococcus (one strain), as well as eight non-identified isolates. CONCLUSIONS: Moderately halophilic bacteria are a source of hydrolytic enzymes such as amylases, DNases, lipases, proteases and pullulanases. SIGNIFICANCE AND IMPACT OF THE STUDY: Although most culture collection strains are not able to produce hydrolases, it has been shown that environmental isolates can produce these potentially biotechnological important enzymes. 相似文献
6.
新疆艾丁湖中度嗜盐苯酚降解菌多样性研究 总被引:1,自引:0,他引:1
高盐含酚废水属于极难处理的废水之一,筛选具有生物学降解能力的嗜盐菌有助于解决这一难题。从新疆艾丁湖盐湖中分离筛选能够降解苯酚的中度嗜盐菌,了解盐湖中度嗜盐苯酚降解菌的多样性组成和降解能力。研究结果表明,10%(质量分数)的盐浓度条件下,分离得到166株嗜盐菌,通过以苯酚为唯一碳源的培养基进行降解活性筛选后得到45株阳性菌,根据细菌16S rRNA基因序列系统进化分析,这45株菌分别归类到3个门,5个科,9个属。其中拟诺卡氏菌属(Nocardiopsis)是优势菌,占总量的68.8%,其余菌分布于Bacillus、Gracilibacillus、Pontibacillus、Halobacillus、Marinococcus和Halomonas属。在含100 mg/L苯酚的液体培养基,经过10 d培养后,这45株菌降解效率为1%~17%。本研究为工业应用提供了嗜盐微生物种质资源,极具进一步发掘和研究价值。 相似文献
7.
采用高盐的牛肉膏蛋白胨培养基(盐浓度为8%NaCl),研究江苏省盐城市盐场土壤里中度嗜盐菌的分布情况及种群特征。从盐城市的射阳、新滩、灌东三处盐场土壤中共采集和分离得到13株中度嗜盐菌。通过形态观察、生理生化分析、16S rRNA序列分析和系统进化分析等方法进行初步鉴定,结果表明:分离到的中度嗜盐菌分属3个属,Virgibacillus属4株、Halomonas属7株和Marinobacter属2株。研究结果揭示盐城市的盐场存在较为丰富的中度嗜盐菌,具有较高的研究和利用价值。 相似文献
8.
四川冬菜中细菌群落组成及多样性 总被引:1,自引:0,他引:1
【目的】了解腌制4年的四川南充冬菜中细菌群落组成及多样性。【方法】通过16S rDNA多样性分析样品细菌落组成;采用16S rDNA-RFLP方法分析从样品中分离出的纯培养细菌。【结果】16S rDNA多样性分析结果表明,样品中细菌主要属于变形杆菌门(Proteobacteria)和厚壁菌门(Firmicutes),分别占克隆文库的87.9%、7.1%,其中包括Virgibacillus kekensis,Marinococcus albus,Salinicoccus sp.,Lactobacillus halophilus和Halomonas等中度嗜盐菌,仅有5%属于放线菌门(Actinobacteria)。通过纯培养方法从冬菜中分离到35株菌,16S rDNA-RFLP分析结果表明,34株属于厚壁菌门(Firmicutes),包括Virgibacillus,Bacillus megaterium和Gracilibacillus saliphilus等中度嗜盐菌,1株属于放线菌门(Actinobacteria)。【结论】冬菜中细菌群落多样性较低,以中度嗜盐菌为主。 相似文献
9.
Badab-e Surt spring is a travertine spring that has low to moderate levels of salt, so it is a good model for isolating moderately halophilic bacteria and investigating the relationship between microbe and environment. For isolating bacterial strains, water and sediment samples were collected from different springheads of the Badab-e Surt spring. Among the 171 bacterial isolates, 110 strains were halophiles. According to comparative partial 16S rRNA sequence analysis, the selected halophilic gram-positive and gram-negative strains were identified as members of the genera: Roseovarius, Labrenzia, Erythrobacter, Erythromicrobium, Massilia, Marinobacter, Halomonas, Shewanella, Pseudomonas, Flavobacterium, Bacillus, Brevibacterium, Staphylococcus, Microbacterium, Kocuria, and Streptomyces. To investigate mineralization, potential strains were screened by the culturing method, and then analyzed with a polarizing and scanning microscope. Five strains, Bss-11a, Bss-3, Bsw-1c1, Bsw-28d, and Bsw-39b, had potential for the mineralization of calcite that very closely resembled species Bacillus cohenii DSM 6307T, Labrenzia aggregate IAM 12614T, Bacillus safensis FO-036bT, Marinobacter flavimaris SW-145T, and Marinobater adhaerens HP15T, respectively. 相似文献
10.
巴里坤湖和玛纳斯湖嗜盐菌的分离及功能酶的筛选 总被引:1,自引:0,他引:1
目的:了解新疆巴里坤湖与马纳斯湖中嗜盐菌及功能酶的多样性。方法:从两湖中采集水样进行菌种分离,采用PCR方法扩增出其16S rRNA基因(16S rDNA),并测定了基因的序列。对分离菌株进行了蛋白酶、淀粉酶、酯酶、脂肪酶、以及纤维素酶的筛选。结果:从两湖水样共分离得到51株嗜盐菌。基于16SrDNA序列的同源性比较和系统发育学分析,发现从两湖分离获得的中度嗜盐菌分别属于Planococcaceae、Bacillacea、Staphylococcus、Halomonadaceae、Salicolaceae以及Pseudomonadacaeae 6个属。分离得到的极端嗜盐古菌属于Halobacteriaceae属。功能酶筛选结果表明产蛋白酶的嗜盐菌共有15株,产酯酶的共有23株,产淀粉酶的共有8株,未获得产脂肪酶和纤维素酶的嗜盐菌。结论:新疆巴里坤湖和马纳斯湖中有丰富的嗜盐微生物资源及酶资源,有重要的研究意义和应用前景。 相似文献
11.
Harmesh Sahay Surendra Singh Rajeev Kaushik Anil K. Saxena Dilip K. Arora 《Biologia》2011,66(5):741-747
Culture dependent phenotypic characterization and 16S rDNA based phylogenetic analyses were applied to study the aerobic halophilic bacterial population present in the Pulicat brackish-water Lake of India. Five different media were employed for isolation of bacteria. A total of 198 morphotypes were recovered, purified and screened for salt tolerance in nutrient agar medium amended with 5–25% NaCl. Based on 16S rDNA restriction fragment length polymorphism analysis with three restriction endonucleases, 51 isolates tolerant to 5% or more NaCl were grouped into 29 clusters. Phylogenetic analysis using 16S rRNA gene sequences revealed that 29 strains could further be allocated into two clades: 19 to Firmicutes and 10 to γ-Proteobacteria. Firmicutes included low G+C Gram-positive bacteria related to family Bacillaceae, which included five genera Bacillus, Virgibacillus, Rummelibacillus, Alkalibacillus and Halobacillus. Another genera included in Firmicutes was Salimicrobium halophilum. In the γ-Proteobacteria group, all the isolates belonged to one genus Halomonas, represented by six different species Halomonas salina, H. shengliensis, H. salifodinae, H. pacifica, H. aquamarina and H. halophila. Most of the isolates exhibited cellulase, xylanase, amylase and protease activities. 相似文献
12.
Meral Birbir Baris Calli Bulent Mertoglu Rahel Elevi Bardavid Aharon Oren Mehmet Nuri Ogmen Ayse Ogan 《World journal of microbiology & biotechnology》2007,23(3):309-316
Tuz Lake is a hypersaline lake located in Central Anatolia, Turkey. The lake and its salterns, Kaldirim and Kayacik, are the
major sources of solar salt for industrial applications in Turkey, especially in the food and leather industries. Use of the
crude solar salt often results in microbial deterioration of the products. We therefore initiated a thorough characterization
of the microbial communities in Tuz Lake and its adjacent salterns, and we present here the results of investigations on diversity
of extremely halophilic Archaea. Twenty-seven colonies of aerobic red or pink Archaea (family Halobacteriaceae) were selected according to colony shape, size, consistency and pigmentation, and characterized according to their phenotypic
characteristics, polar lipid contents, and antibiotic sensitivities. Furthermore, 16S rRNA genes of the isolates were screened
by DGGE analysis and partially sequenced. Phylogenetic analysis showed that most isolates belonged to the genera Haloarcula, Halorubrum and Halobacterium. Haloarcula was found to be dominant both in Tuz Lake and in the saltern samples. Halorubrum species were isolated from Tuz Lake and from the Kaldirim saltern, and Halobacterium species were recovered from Tuz Lake and from the Kayacik saltern. All strains showed various activities of hydrolytic enzymes
(proteases, amylases, cellulases, and others), activities which are responsible for the detrimental effects of the crude salt
in food and leather products. 相似文献
13.
Birbir Meral Ogan Ayse Calli Baris Mertoglu Bulent 《World journal of microbiology & biotechnology》2004,20(6):613-621
Tuzkoy is a large and unpolluted salt mine in Central Anatolia, Turkey. High quality salt in this mine may be supplied to
the table salt market after a simple process instead of complicated refining. However, the extremely halophilic microbial
content of the salt has not yet been investigated which may result in detrimental effects in industrial processes such the
food and leather industries, if unprocessed salt is directly used. Therefore, to identify and characterize the microbial contaminants
in Tuzkoy salt, chemical and microbial analyses were conducted on salt crystal samples collected from three different locations
of the mine. Generally 105–106 colony forming units of extremely halophilic microorganisms were detected per gram of salt samples. Twelve colonies were
selected randomly for further characterization. Phenotypic characterization, lipid analysis, antibiotic susceptibility tests
and positive PCR amplification results with Archaea-specific primers confirmed that all strains were halophilic Archaea belong to family Halobacteriaceae. According to denaturing gradient gel electrophoresis (DGGE) results, 10 comparatively different strains were selected for
DNA sequencing. DNA sequences and phylogenetic analyses revealed that the isolated strains were mainly species of the genera
Halobacterium, Haloarcula, Natrinema and Halorubrum. In addition enzymatic activity tests were also conducted to evaluate the salt quality for industrial applications. Results
of gelatinase, caseinase, amylase, cellulase and lipase activity tests revealed that the isolated strains produced hydrolytic
enzymes, which could cause deterioration in salt-treated food and hide. It may be pointed out that cellulase activity in halophilic
Archaea has not been reported previously. Moreover, β-galactosidase activity has been reported in some Haloferax and Halorubrum species but not in the genus Halobacterium.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
14.
Hamid Babavalian Mohammad Ali Amoozegar Shirin Zahraei Rokhsareh Rohban Fatemeh Shakeri Mehrdad Moosazadeh Moghaddam 《Indian journal of microbiology》2014,54(4):444-449
This research is a comparative study on the diversity of halophilic bacteria with hydrolytic activities in three significant hypersaline lakes; Urmia in the northwest and Howz-Soltan and Aran-Bidgol in the central desert in Iran. Isolated strains from these saline lakes were found to be halotolerant, moderately and extremely halophilic bacteria. The bacteria in each saline lake were able to produce different hydrolytic enzymes including amylase, protease, lipase, DNase, inulinase, xylanase, carboxy methyl cellulase, pectinase and pullulanase. 188, 302, 91 halophilic strains were isolated from Urmia Lake, Howz-Soltan and Aran-Bidgol playa, respectively. The numbers of Gram-positive strains were more than Gram-negatives, and among Gram-positive bacteria; spore-forming bacilli were most abundant. Due to the unique physico-chemical conditions of the lake environments, the hydrolytic activities of isolated strains were significantly different. For instance, isolated strains from Howz-Soltan playa did not produce pectinase, DNase, amylase, lipase and inulinase, while the isolates from Aran-Bidgol playa had a great ability to produce pectinase and DNase. The strains from Urmia Lake were also good producers of DNase but failed to show any chitinase activity. The diversity of halophilic bacteria from the mentioned three saline lakes was also determined using PCR-amplified 16S rRNA followed by phylogenetic analysis of the partial 16S rRNA sequences.
Electronic supplementary material
The online version of this article (doi:10.1007/s12088-014-0481-9) contains supplementary material, which is available to authorized users. 相似文献15.
María de Lourdes Moreno María Teresa García Antonio Ventosa & Encarnación Mellado 《FEMS microbiology ecology》2009,68(1):59-71
In order to explore the diversity of extreme halophiles able to produce different hydrolytic enzymes (amylase, protease, lipase and DNAse) in hypersaline habitats of South Spain, a screening program was performed. A total of 43 extreme halophiles showing hydrolytic activities have been isolated and characterized. The isolated strains were able to grow optimally in media with 15–20% (w/v) total salts and in most cases, growth was detected up to 30% (w/v) total salts. Most hydrolase producers were assigned to the family Halobacteriaceae , belonging to the genera Halorubrum (22 strains), Haloarcula (nine strains) and Halobacterium (nine strains), and three isolates were characterized as extremely halophilic bacteria (genera Salicola, Salinibacter and Pseudomonas ). An extremely halophilic isolate, strain IC10, showing lipase and protease activities and identified as a Salicola strain of potential biotechnological interest, was further studied. The optimum growth conditions for this strain were 15–20% (w/v) NaCl, pH 8.0, and 37 °C. Zymographic analysis of strain IC10 detected the lipolytic activity in the intracellular fraction, showing the highest activity against p -nitrophenyl-butyrate as a substrate in a colorimetric assay, whereas the proteolytic activity was detected in the extracellular fraction. This protease degraded casein, gelatin, bovine serum albumin and egg albumin. 相似文献
16.
Mădălin Enache Takashi Itoh Masahiro Kamekura Gabriela Popescu Lucia Dumitru 《Central European Journal of Biology》2008,3(4):388-395
We isolated a number of extremely halophilic archaea from four salt lakes (Red Bath, Green Bath, Shepherd Bath and Bride Cave)
located in Slanic Prahova, Romania. The characterization of 12 representative isolates by polyphasic approach revealed that
11 strains were members of the genus Haloferax and only one was a member of the genus Haloarcula. The 11 Haloferax isolates possessed sulfated diglycosylarchaeol-1 as the major membrane glycolipid, and G+C contents of total DNA were 63.4–65.8
mol%. The predominant isolation of Haloferax species from the lakes may suggest that the underground salt deposit possesses Haloferax species as the major biota of ancient origin. To the best of the authors’ knowledge, this is the first paper on the survey
of halophilic archaea of man-made young salt lakes. 相似文献
17.
Furkan Orhan 《Geomicrobiology journal》2013,30(1):10-16
In the current study, 18 salt-tolerant bacteria were isolated from salt-affected soil in the east Anatolian region. The obtained isolates were identified and characterized by conventional (morphology, physiology, and biochemical tests) and molecular techniques 16 rDNA. Among 18 sequenced isolates, 6 Bacillus, 1 Halomonas, 2 Halobacillus, 2 Zhihengliuella, 2 Oceanobacillus, 1 Virgibacillus, 1 Staphylococcus, 1 Thalassobacillus, 1 Exiguobacterium were identified with high similarity to previously identified strains in the literature. According to the results obtained, investigated bacterial strains have high salt tolerance and significant enzyme activities that can improve soil nutrient cycling and fertility. To the best of our knowledge, the current article is the first study in evaluation and diversity of potential halophlic/halotolerant bacterial strains in salt-affected soils of the east Anatolian region. 相似文献
18.
B. Essghaier M.L. Fardeau J.L. Cayol M.R. Hajlaoui A. Boudabous H. Jijakli N. Sadfi-Zouaoui 《Journal of applied microbiology》2009,106(3):833-846
Aims: Grey mould caused by Botrytis cinerea is an economically important disease of strawberries in Tunisia and worldwide. The aim of this study was to select effective halophilic bacteria from hypersaline ecosystems and evaluate the abilities of antifungal bacteria to secrete extracellular hydrolytic enzymes, anti- Botrytis metabolites and volatiles.
Methods and Results: Grey mould was reduced in strawberry fruits treated with halophilic antagonists and artificially inoculated with B. cinerea . Thirty strains (20·2%) were active against the pathogen and reduced the percentage of fruits infected after 3 days of storage at 20°C, from 50% to 91·66%. The antagonists were characterized by phenotypic tests and 16S rDNA sequencing. They were identified as belonging to one of the species: Virgibacillus marismortui , B. subtilis , B. pumilus , B. licheniformis , Terribacillus halophilus , Halomonas elongata , Planococcus rifietoensis , Staphylococcus equorum and Staphylococcus sp. The effective isolates were tested for antifungal secondary metabolites.
Conclusions: Moderately halophilic bacteria may be useful in biological control against this pathogen during postharvest storage of strawberries.
Significance and Impact of the study: The use of such bacteria may constitute an important alternative to synthetic fungicides. These moderate halophiles can be exploited in commercial production and application of the effective strains under storage and greenhouse conditions. 相似文献
Methods and Results: Grey mould was reduced in strawberry fruits treated with halophilic antagonists and artificially inoculated with B. cinerea . Thirty strains (20·2%) were active against the pathogen and reduced the percentage of fruits infected after 3 days of storage at 20°C, from 50% to 91·66%. The antagonists were characterized by phenotypic tests and 16S rDNA sequencing. They were identified as belonging to one of the species: Virgibacillus marismortui , B. subtilis , B. pumilus , B. licheniformis , Terribacillus halophilus , Halomonas elongata , Planococcus rifietoensis , Staphylococcus equorum and Staphylococcus sp. The effective isolates were tested for antifungal secondary metabolites.
Conclusions: Moderately halophilic bacteria may be useful in biological control against this pathogen during postharvest storage of strawberries.
Significance and Impact of the study: The use of such bacteria may constitute an important alternative to synthetic fungicides. These moderate halophiles can be exploited in commercial production and application of the effective strains under storage and greenhouse conditions. 相似文献
19.
Lei Chen Guangyu Wang Tong Bu Yunbin Zhang Yixin Wang Ming Liu Xiukun Lin 《World journal of microbiology & biotechnology》2010,26(5):879-888
A total of 45 moderately halophilic bacteria was isolated from sediment and saline water collected from the Weihai Solar Saltern
(China). The phylogenetic position of all the isolated strains was determined by 16S rRNA sequencing. The halophilic strains
were tested for their antimicrobial activity. Cytotoxicity assay was performed to determine which of the halophilic strains
could inhibit proliferation of human hepatocellular carcinoma Bel 7402 cells. Our results showed that all of the isolated
45 strains displayed moderately halophilic characteristics. Phylogenetic analysis indicated that 17 of the isolated strains
were related to the phylum Firmicutes and belonged to four genera, Bacillus, Halobacillus, Planococcus and Salinicoccus. The other strains identified as genus of Halomonas belonged to phylum γ-Proteobacteria. Most of the halophilic bacterial strains showed potent activities against Gram-positive
bacteria, human pathogenic fungi and plant pathogenic fungi. In addition, the crude extracts from 14 halophilic bacterial
strains showed cytotoxic activity against tumor cells Bel 7402, and five of them showed remarkable activities with IC50 less than 40 μg ml−1. Our results suggest that the moderately halophilic bacteria may be developed as promising sources for the discovery of novel
bioactive substances. 相似文献
20.
Joshi AA Kanekar PP Kelkar AS Shouche YS Vani AA Borgave SB Sarnaik SS 《Microbial ecology》2008,55(2):163-172
Aerobic, alkaliphilic bacteria were isolated and characterized from water and sediment samples collected in the winter season,
January 2002 from alkaline Lonar lake, India, having pH 10.5. The total number of microorganisms in the sediment and water
samples was found to be 102–106 cfu g−1 and 102–104 cfu ml−1, respectively. One hundred and ninety-six strains were isolated using different enrichment media. To study the bacterial
diversity of Lonar lake and to select the bacterial strains for further characterization, screening was done on the basis
of pH and salt tolerance of the isolates. Sixty-four isolates were subjected to phenotypic, biochemical characterization and
16S rRNA sequencing. Out of 64, 31 bacterial isolates were selected on the basis of their enzyme profile and further subjected
to phylogenetic analysis. Phylogenetic analysis indicated that most of the Lonar lake isolates were related to the phylum
Firmicutes, containing Low G+C, Gram-positive bacteria, with different genera: Bacillus, Paenibacillus, Alkalibacillus, Exiguobacterium, Planococcus, Enterococcus and Vagococcus. Seven strains constituted a Gram-negative bacterial group, with different genera: Halomonas, Stenotrophomonas and Providencia affiliated to γ-Proteobacteria, Alcaligenes to β-Proteobacteria and Paracoccus to α-Proteobacteria. Only five isolates were High G+C, Gram-positive bacteria associated with phylum Actinobacteria, with
various genera: Cellulosimicrobium, Dietzia, Arthrobacter and Micrococcus. Despite the alkaline pH of the Lonar lake, most of the strains were alkalitolerant and only two strains were obligate alkaliphilic.
Most of the isolates produced biotechnologically important enzymes at alkaline pH, while only two isolates (ARI 351 and ARI
341) showed the presence of polyhydroxyalkcanoate (PHA) and exopolysaccharide (EPS), respectively. 相似文献