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1.
燕红  钟方  高新亮  刘蕊 《生态学杂志》2012,31(4):1000-1008
将采集于黑龙江省大庆市盐碱地的土壤样品经适当稀释后,涂布于高盐碱的培养基中,分离纯化获得41株菌。经过不断分别或同时提高培养基的盐、碱浓度进行耐盐碱菌株的筛选,获得1株耐盐碱菌株7,其在pH值13且盐(NaCl)浓度达到200g.L-1的培养基中仍可生长,该菌株既属于极端嗜盐微生物又属于极端嗜碱微生物。分别于不同碳源、氮源、碳氮比、初始pH值和培养温度条件下,对菌株7的生物学特性和盐、碱去除能力进行研究。结果表明,该菌株生长和去除盐碱的最佳碳源为牛肉膏;最佳氮源为蛋白胨;碳氮比为4:1时,最有利于菌株的生长和对盐、碱的去除;菌株生长的最适初始pH值为9.5,最适温度为20℃,而盐、碱去除的最佳温度则为30~35℃。  相似文献   

2.
为了比较分析盐碱土壤与非盐碱土壤微生物资源抗盐碱性差异,本研究利用含有不同浓度Na2CO3、NaHCO3和pH的培养基对盐碱土壤和非盐碱土壤细菌进行培养计数.结果显示:非盐碱土壤出菌数量随Na2CO3、pH和NaHCO3浓度升高而下降,盐碱土壤细菌出菌数量随着Na2CO3、pH和NaHCO3浓度升高先是升高然后下降,最高值分别出现在200 mmol/L NaHCO3、50 mmol/L Na2CO3和pH 9.0的分离平板上.此外,高Na2CO3、pH和NaHCO3浓度的平板中盐碱土壤出菌数量远高于非盐碱土壤;以上结果可见,耐盐碱细菌资源主要集中分布在盐碱土壤中,在非盐碱土壤中虽有分布,但是仅占有很少一部分.  相似文献   

3.
盐碱协同胁迫对向日葵抗氧化酶系统的影响   总被引:2,自引:0,他引:2  
根据中国东北盐碱土壤特点,将4种盐NaCl、NaHCO3、Na2SO4和Na2CO3按不同比例混合,模拟出25种盐度和pH值各不相同的复杂盐碱条件(盐浓度为50~250 mmol/L,pH值为712~1046),并对向日葵苗进行盐碱混合胁迫处理,研究了向日葵超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)酶等抗氧化酶系统和丙二醛(MDA)的盐碱协同胁迫效应.结果表明, 向日葵抗氧化物酶活性强弱同时与盐度和碱度密切相关,3种抗氧化物酶活性对于盐浓度的反应相似,均为其含量随着盐浓度的升高开始逐渐升高然后下降,而对于pH的影响,不同酶反应结果不同.即随着pH值升高,SOD酶活性和CAT酶活性降低,而POD酶活性反应则是随着pH值升高活性也升高.双向方差分析(ANOVA)结果表明:盐碱效应对于3种酶活力的影响是显著的.其中,盐效应对POD和SOD活性的影响比pH值的影响大,而pH值对CAT活性的影响效应比盐效应大.除SOD外,盐碱效应的交互作用显著 (P<0001).抗氧化酶系统和MDA含量两者间相关性和逐步回归分析表明,3种酶对MDA的影响效应随其强度不同呈现显著不同.其中SOD是1个主导因子,CAT 处于次位, 而POD的影响不大,甚至可以忽略.  相似文献   

4.
塔里木盆地荒漠盐碱生境土壤放线菌的初步研究   总被引:3,自引:0,他引:3  
采用常规方法研究了新疆塔里木盆地荒漠盐碱生境土壤放线菌区系、放线菌的耐盐、碱性及对植物病原真菌的拮抗性。结果表明:土壤放线菌区系较单一,仅分离到链霉菌属和拟诺卡氏菌属,其中以链霉菌属为主(98.57%),链霉菌可分10个类群,以白孢类群为优势类群。土壤含盐量愈高,链霉菌组成愈简单。放线菌的最适生长盐浓度多集中在6%~8%,最适生长pH集中在7~8。24株供试放线菌中,有4株中度嗜盐放线菌对供试靶标菌有拮抗性,占供试菌的16.67%。  相似文献   

5.
混合盐碱胁迫对芹菜种子萌发的影响   总被引:2,自引:0,他引:2  
NaCl、Na2SO4、NaHCO3和Na2CO3按不同比例混合,模拟盐度和pH变化规律与天然盐碱地相似的15种盐碱条件,探讨混合盐碱胁迫对芹菜(Apium graveolens)种子萌发的影响.结果表明:芹菜种子的发芽率、发芽势、发芽指数和活力指数均随盐浓度的升高,pH的增大呈下降趋势.芹菜种子的萌发主要受盐浓度的影响,不同盐浓度间的影响差异大;当盐浓度为200 mmol/L时,基本不萌发.  相似文献   

6.
应用逐步回归法,分析了5~9月松嫩盐碱草地植物群落的生物量、丰富度、多样性和均匀度与同月土壤因子间的关系。松嫩盐碱草地植物群落特征受若干土壤因子的共同影响,不同月份影响因子的变化较复杂。5~7月土壤盐分和养分共同影响着松嫩盐碱草地植物群落生物量,但是土壤盐分因子与群落生物量的相关性更明显(5月的Ca2+质量分数,6月和7月的Mg2+质量分数)。土壤盐分因子在整个生长季内对松嫩盐碱草地植物群落生物量的直接作用都大于土壤养分因子的。6月时影响松嫩盐碱草地植物群落丰富度、Simpson指数和Shannon指数的土壤养分因子增加,其中土壤全氮质量分数的直接作用是所有土壤因子中最大的。8月植物群落生物量、Simpson指数和Shannon指数仅受土壤盐分因子影响,其中生物量与土壤pH值以及Simpson指数与土壤碱化度都是极显著负相关,Shannon指数与土壤含盐量显著负相关。6~9月的松嫩盐碱草地植物群落均匀度也同时受土壤养分和盐分因子的作用,其中6月和9月时土壤盐分因子对群落均匀度的直接作用更突出。生长末期(9月)松嫩盐碱草地植物群落生物量与土壤因子间的关系以及生长初期(5月)植物群落均匀度与土壤因子间的关系都不明显。  相似文献   

7.
新疆艾丁湖及邻近地区嗜盐菌和耐盐菌的数量分布   总被引:12,自引:0,他引:12  
以盐(NaCI)为限制性生物因子,对艾丁湖地区原核生物的分布状态进行调查,从距艾丁湖中心1.5km处出发,定距离,分季节取土样及水样(共取样20个)。对样品菌群数量统计表明,在湖水中,春秋两嗜盐菌的数量高于耐盐菌。冬季湖水温度大大降低,盐结晶沉淀,湖水中的盐浓度降低,使得湖水中的耐盐菌的数量高于嗜盐菌的数量。土壤中的有要物比湖水中的丰富,温度也较稳定,因而耐盐菌的数量高于嗜盐菌的数量。距湖岩越远,土壤中对盐依赖性强的细菌越少,反之对盐依赖性弱的菌越多。由样品中分离得到细菌173株,放线菌12株,以不同浓度NaCI为唯一生长限制因子对所分离到的细菌进行生理生化实验,获得嗜盐菌61株,其中除3株可视为新种外(新种的鉴定将另外报告),其它均为嗜盐菌的已知产生菌种,现仅报道艾丁湖及邻近地区以盐为限制因子的原核微生物分布。  相似文献   

8.
大黄鱼细菌性病原哈维氏弧菌培养特性的研究   总被引:1,自引:0,他引:1  
弧菌是危害水产养殖动物的最为严重的病原之一.从患病大黄鱼肝脏分离致病的细菌哈维氏弧菌GYC1108-1为材料,对哈维氏弧菌GYC1108-1株的最佳生长条件及培养基优化进行了测定.综合考察不同的培养温度、培养时间、培养基盐度、培养基的pH值对细菌生长的影响,并采用正交实验法,对培养基配方进行优化.结果表明:不同的培养温度、培养时间、培养基盐度、培养基pH值等均会影响细菌的产量.GYC1108-1适宜生长的盐度为1%-5%、pH为5~9.5、温度为15~35℃、在培养基中添加硫酸铜、硫酸氨会明显促进生长;最佳培养条件及培养基成份为:NaCl 2%,pH8.0,温度30℃,蛋白胨1.0%、牛肉膏0.75%、CuSO4 0.3mg/L、(NH4)2SO4 0.1g/L.  相似文献   

9.
陈萍  夏江宝  王善龙  李栋  高芳磊  赵万里  马金昭 《生态学报》2022,42(24):10180-10190
为探讨黄河三角洲滨海滩涂不同密度柽柳林的土壤盐碱和养分特征,明确不同密度柽柳林的“盐谷”及“肥岛”效应,以黄河三角洲山东省滨州市北海新区滨海滩涂的低密度(1100 株/hm2)、中密度(4100 株/hm2)和高密度(7100 株/hm2)柽柳林为研究对象,测定分析不同密度柽柳林及柽柳植株周边不同位置的土壤pH、电导率、速效氮、速效磷、速效钾、有机碳等指标。结果表明:(1)土壤电导率随林分密度增大表现为逐渐减小,在中、高密度柽柳林,土壤电导率分别比低密度降低28.39%、55.74%;随距离柽柳植株远近不同,中、高密度柽柳林土壤电导率表现为根部<冠幅中心<冠幅边缘<株间空地,即中、高密度柽柳林出现“盐谷”效应,而低密度林分未出现。(2)土壤速效氮、速效磷和有机碳含量随林分密度增大表现出先增大后减小,在中、高密度柽柳林,柽柳植株周边不同位置的速效氮、速效磷和有机碳含量存在显著差异(P<0.05),表现为根部>冠幅中心>冠幅边缘>株间空地,而低密度柽柳林差异不显著(P>0.05);即中、高密度柽柳林出现“肥岛”效应,而低密度林分未出现。(3)中密度柽柳林在养分含量、养分富集率方面高于高密度,在盐分富集率方面低于高密度,具有更强的“肥岛”、“盐谷”效应。(4)不同密度柽柳林以及柽柳植株周边不同位置的土壤pH差异均不显著(P>0.05)。林分密度显著影响土壤盐分及养分含量,中密度柽柳林具有显著提高土壤肥力的作用,高密度次之,而低密度较差。不同密度柽柳林的降盐作用表现为中、高密度较好,低密度较差。基于柽柳林改良土壤盐碱与养分的作用,建议黄河三角洲滨海滩涂柽柳合理的初植密度为4100 株/hm2。  相似文献   

10.
施加改良剂对重度盐碱地盐碱动态及杨树生长的影响   总被引:16,自引:0,他引:16  
如何控制盐碱地继续朝向重度盐碱地发展并恢复森林等植被生态系统是国家科技攻关重点方向,也是内陆碳酸钠型盐碱地改良研究的一个重点,以聚马来酸酐(HPMA)和聚丙烯酸(PAA)两种高分子聚合物配合木焦油、木醋液等为降、阻盐碱剂,对重度盐碱地进行改良,以杨树生长情况以及不同层盐碱动态为指标判断其对重度盐碱地的改良效果.结果表明,(1)HPMA与PAA的比较,土壤改良剂聚马来酸酐(HPMA)在阻盐剂(木醋液+木焦油+少量PAA与碱土形成的阻隔盐碱向表层运输的阻盐碱层)阻隔下,使盐碱地pH与盐分明显下降(与对照相比pH下降0.93、盐分下降0.78%),杨树生长速率较高(株高生长速度是对照生长速度的2.41倍、基茎长速度是对照生长速度的2.36倍),改良效果显著.土壤改良剂聚丙烯酸(PAA)与碱土混合在阻盐剂阻隔下, pH与盐分变化较小(与对照相比pH下降0.23、盐分下降0.63%),杨树生长速率缓慢(株高生长速度是对照生长速度的1.43倍、基茎长速度是对照生长速度的1.07倍),较聚马来酸酐处理稍差.(2)加覆膜剂与不加覆膜剂对比发现,加覆膜剂后土壤表层pH值降至8.87,而其他土层pH值无明显变化,覆膜剂对盐碱的作用主要体现在土壤深层与浅层之间的盐碱动态,对深层盐分没有明显抑制作用.(3)通过对不同改良方法根际土与远根土对比发现,经HPMA和PAA处理后根际土壤盐分和pH值均较比远根土降低,说明种植杨树能够强化改良剂的效果.  相似文献   

11.
Two hundred forty-three isolates of alfalfa nodule bacteria (Sinorhizobium meliloti) were obtained from legume nodules and soils sampled in the northern Aral region, experiencing secondary salinization. Isolates obtained from nodules (N isolates) were significantly more salt-tolerant than those from soils (S isolates) when grown in a liquid medium with 3.5% NaCl. It was found that wild species of alfalfa, melilot, and trigonella preferably formed symbioses with salt-tolerant nodule bacteria in both salinized and nonsalinized soils. Only two alfalfa species, Medicago falcata and M. trautvetteri, formed efficient symbioses in soils contrasting in salinity. The formation of efficient symbiosis with alfalfa in the presence of 0.6% NaCl was studied in 36 isolates (N and S) differing in salt tolerance and symbiotic efficiency. Fifteen isolates formed efficient symbioses in the presence of salt. The increase in the dry weight of the plants was 25-68% higher than in the control group. The efficiency of symbiotic interaction under salinization conditions depended on the efficiency of the isolates under standard conditions but did not correlate with the source of nodule bacteria (soil or nodule) or their salt tolerance. The results indicate that nodule bacterium strains forming efficient symbioses under salinization conditions can be found.  相似文献   

12.
Cementation of salt-containing soils can be achieved by salt-tolerant or halophilic calcite precipitation bacteria. Therefore, the isolation of calcite-producing bacteria in the presence of salt is the first step in the microbial cementation of saline soils. Urease producing bacteria can cause calcite nano-crystals to precipitate by producing urease in the presence of urea and calcium. The purpose of this study was to isolate urease producing halophilic bacteria in order to make calcite precipitate in saline soil. The calcite and the properties of the strains were further analyzed by X-ray diffraction (XRD) and scanning electron microscope equipped with an energy dispersive X-ray detector. In this study, a total of 110 halophilic strains were isolated, from which 58 isolates proved to have the ability of urease production. Four strains were identified to produce nano-calcite using urease activity in the precipitation medium. The XRD studies showed that the size of these particles was in the range of 40–60 nm. Strain H3 revealed that calcite is mostly produced in the precipitation medium containing 5% salt in comparison with other strains. This strain also produced calcite precipitates in the precipitation medium containing 15% salt. Phylogenetic analysis indicated that these isolates are about 99–100% similar to Staphylococcus saprophyticus.  相似文献   

13.
Two hundred forty-three isolates of alfalfa root-nodule bacteria (Sinorhizobium meliloti) were obtained from nodules and soils sampled in the northern Aral region, experiencing secondary salinization. Isolates obtained from nodules (N isolates) were significantly more salt-tolerant than those from soils (T isolates) when grown in a liquid medium with 3.5% NaCl. It was found that wild species of alfalfa, melilot, and trigonella preferably formed symbioses with salt-tolerant root-nodule bacteria in both salinized and nonsalinized soils. Only two alfalfa species, Medicago falcata and M. trautvetteri, formed efficient symbioses in soils contrasting in salinity. The formation of efficient symbiosis with alfalfa in the presence of 0.6% NaCl was studied in 36 isolates (N and T) differing in salt tolerance and symbiotic efficiency. Fifteen isolates formed efficient symbioses in the presence of salt. The increase in the dry weight of the plants was 25–68% higher than in the control group. The efficiency of symbiotic interaction under salinization conditions depended on the symbiotic efficiency of the isolates under standard conditions but did not correlate with the source of root-nodule bacteria (soil or nodule) or their salt tolerance. The results indicate that the strains of root-nodule bacteria forming efficient symbioses under salinization conditions can be found.  相似文献   

14.
【目的】通过对吉尔吉斯共和国比什凯克地区一处盐碱土壤样品可培养细菌的分离筛选,初步了解该地区土壤微生物生理多样性和系统发育多样性。【方法】利用加盐(NaCl 5%)的R2A、TSB、1/4×NA和Gause No.1培养基筛选耐盐碱菌株。对部分分离菌株的革兰氏染色、耐盐性、生长温度范围、pH耐受、产酶性能进行了比较,进而采用核糖体DNA扩增片段限制性酶切分析(ARDRA)研究了比什凯克地区耐盐碱细菌的群落结构和多样性。【结果】从比什凯克地区盐碱土样中分离得到120株耐盐碱细菌,通过限制性内切酶Hae III对纯化菌株的16S rRNA基因进行酶切分型,根据ARDRA的酶切图谱,将其划分为19个操作分类单元。16S rRNA基因序列测定和系统发育分析结果显示,分离菌株分布于厚壁菌门、放线菌门、变形菌门下的17个种属,且部分菌株的16S rRNA基因序列同源性低于97%,可能是潜在的新种。【结论】比什凯克地区盐碱土样中的耐盐碱细菌不仅具有丰富的多样性,还蕴藏着具有地域特点的新菌种资源。  相似文献   

15.
Types and properties of some bacteria isolated from hypersaline soils   总被引:4,自引:2,他引:2  
Five rhizosphere soil samples from the dominant xerophytic plants, and nearby root-free soil samples were obtained from a series of hypersaline soils (5.0–10.7% NaCl) from sites near Alicante in Spain. Physico-chemical analyses were made, and the bacterial flora estimated using three different plating media. Counts from rhizosphere soil were always significantly higher than those from root-free soils. A total of 211 strains isolated were purified and identified to genus level; 12 could not be classified. The range of salt concentration allowing growth was determined for each isolate, but this did not correlate with the salt content of the soil habitat. Most isolates appeared to be typical moderate halophiles (with optimum growth between 5 and 15% salts), but about half of them grew on normal media with only 0.9% naCl, a notable difference from moderately halophilic aquatic bacteria. Extreme halophiles were rare but this may have been due to an insufficient incubation period.  相似文献   

16.
嗜酸丝状放线菌的选择性分离与多样性   总被引:3,自引:0,他引:3  
摘要:【目的】针对酸性土壤中的嗜酸丝状放线菌,建立有效的选择性分离方法,并了解其多样性。【方法】用不同的样品预处理方式和分离培养基,并添加不同的抑制剂进行分离;根据放线菌的菌落数和出菌率确定最佳分离方法组合。采用最佳分离方法对从江西采集的17份酸性土壤样品进行分离;根据培养特征对分离菌株进行分群,进一步通过对各类群的显微形态观察和pH梯度生长实验确定代表菌株;对代表菌株进行16S rRNA基因序列分析研究其多样性。【结果】嗜酸丝状放线菌的最佳分离方法为:土壤样品经分散差速离心预处理后,涂布添加了放线菌酮、制霉菌素和萘啶酮酸(各50 mg/L)的GTV培养基。用此方法共分离到放线菌369株,归为10个不同的颜色类群,其中6.6%为严格嗜酸放线菌,72.4%为中度嗜酸放线菌,21.0%为耐酸放线菌。52株嗜酸放线菌代表菌株分布于放线菌目中的12个属:链霉菌属(Streptomyces)、小单孢菌属(Micromonospora) 、诺卡氏菌属(Nocardia)、野野村菌属(Nonomuraea) 、韩国生工属(Kribbella) 、小双孢菌属(Microbispora)、马杜拉菌属(Actinomadura)、拟无枝菌酸菌属(Amycolatopsis)、指孢囊菌属(Dactylosporangium)、伦茨氏菌属(Lentzea)、游动四孢菌属(Planotetraspora) 和链嗜酸菌属(Streptacidiphilus),其中链霉菌分离菌株在系统发育树上形成12个不同的进化类群。【结论】所建立的选择性分离方法可用于土壤嗜酸丝状放线菌的高效分离;江西酸性土壤含有丰富多样的嗜酸丝状放线菌种属。  相似文献   

17.
Rogosa broth, without its salt supplement and dissolved in deionized water, was adapted for the selective isolation and enumeration of enterococci. This medium supported good growth of enterococci, but it suppressed growth of other lactic acid bacteria. The sensitivity and specificity of the medium were tested after addition of various increasing concentrations of NaN(3) against known strains of enterococci and other bacteria. Many strains of Streptococcus faecium showed low azide tolerance; optimal growth was obtained at a concentration of 0.01% NaN(3), which totally or partially inhibited unrelated species of lactic acid bacteria. The selectivity of the medium was further increased by pH adjustment to 9.6. Carbonate and Tween 80 were added to overcome partial inhibition of enterococcal growth by the new combination of selective conditions. The final medium was evaluated in agar form in isolations from human and animal feces, polluted water, meat, and dairy products. Counts were obtained after 16 to 17 h of incubation at 37 C. The isolates satisfactorily conformed to the group characteristics of enterococci.  相似文献   

18.
Re‐establishing native vegetation in stressed soils is of considerable importance in many parts of the world, leading to significant interest in using plant–soil symbiont interactions to increase the cost‐effectiveness of large‐scale restoration. However, effective use of soil microbes in revegetation requires knowledge of how microbe communities vary along environmental stress gradients, as well as how such variation relates to symbiont effectiveness. In Australia, shrubby legumes dominate many ecosystems where dryland salinity is a major issue, and improving plant establishment in saline soils is a priority of regional management agencies. In this study, strains of rhizobial bacteria were isolated from a range of Acacia spp. growing in saline and non‐saline soils. Replicates of each strain were grown under several salinity levels in liquid culture and characterized for growth and salt tolerance. Genetic characterization of rhizobia showed considerable variation among strains, with salt tolerance and growth generally higher in rhizobial populations derived from more saline soils. These strains showed markedly different genetic profiles and generic affiliations to those from more temperate soils, suggesting community differentiation in relation to salt stress. The identification of novel genomic species from saline soils suggests that the diversity of rhizobia associated with Australian Acacia spp. is significantly greater than previously described. Overall, the ability of some symbiotically effective strains to tolerate high salinity is promising with regard to improving host plant re‐establishment in these soils.  相似文献   

19.
Soil bacteria may have properties of plant growth promotion but not be sufficiently beneficial for plants under stress conditions. This challenge has led researchers to extend their searches into extreme environments for potential soil bacteria with multiple plant beneficial traits as well as abiotic stress tolerance abilities. In the current study, an attempt was made to evaluate soil bacteria from an extreme environment, volcano soils, based on plant growth promoting and abiotic stress mitigating characteristics. The screening led to the isolation of eight (NBRISH4, NBRISH6, NBRISH10, NBRISH11, NBRISH13, NBRISH14, NBRISH16 and NBRISH26) bacterial isolates capable of withstanding stresses, namely temperature (up to 45 °C), salt (up to 2 M NaCl) and drought (up to 60% Poly Ethylene Glycol 6000) in vitro. Further, the selected isolates were notable for their in vitro temporal performance with regards to survival (in terms of colony count), phosphate solubilisation, biofilm formation, auxin, alginate and exo-polysaccharide production abilities under abiotic stresses i.e. 40 °C temperature; 500 mM NaCl salt and drought (PEG) conditions. In vivo seed treatments of individual selected bacteria to maize plants resulted into significant enhancement in root and shoot length, root and shoot fresh and dry weight and number of leaves per plant. Overall, the plant growth promoting and abiotic stress tolerance ability was most evident for bacterial isolate NBRISH6 which was identified as an Ochrobactrum sp. using 16S rRNA based phylogenetic analysis.  相似文献   

20.
溶磷真菌的筛选及耐盐特性分析   总被引:1,自引:0,他引:1  
【背景】土壤盐渍化已成为影响土壤质量和作物产量的重要因素之一,利用微生物改良盐渍化土壤是既经济又环保的方法。【目的】从不同土壤样品和生物肥料中筛选溶磷能力较强的真菌并讨论其耐盐能力,为盐渍化土壤改良提供菌种资源。【方法】采用平板培养法筛选有一定溶磷能力的真菌,经ITS序列分析初步确定菌株的分类地位。以溶磷能力为考察指标,以NaCl梯度和磷酸钙梯度为考察因素,分析不同菌株利用无机磷源的能力,以及溶磷能力与pH的关系。【结果】共筛选得到16株具有较强溶磷能力的真菌,其中4株真菌对水稻发芽有显著的促进作用,它们是1株长枝木霉(MF-1)和3株踝节菌属菌株(SD-2、XJ-7和HLJ-3)。菌株SD-2和XJ-7生长的耐盐能力显著好于菌株MF-1和HLJ-3。当NaCl浓度为5%时,菌株XJ-7的溶磷能力最好,溶磷率可达9.50%;当NaCl浓度为1%时,菌株HLJ-3的溶磷能力较好,溶磷率为6.93%;当NaCl浓度为0时,菌株SD-2和MF-1的溶磷能力较强,溶磷率分别为9.07%和3.73%。进一步研究发现踝节菌属真菌的溶磷能力与菌液pH呈显著负相关关系。【结论】筛选获得的4株真菌其溶磷能力在不同盐环境中有显著差异,为今后土壤改良和生物肥料中菌种的选择提供理论依据和试验基础。  相似文献   

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