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1.
以大豆下胚轴为试验材料,研究了绿原酸(CGA)及其与植物生长调节剂(IAA、IBA)共同作用对大豆下胚轴不定根发生的影响。结果表明,当CGA施用量达到200μmol/L及400μmol/L时,大豆下胚轴生根率分别显著降低了17.8%和26.7%,平均根长分别显著降低了18.7%和35.4%;当植物生长调节剂施用量为2μmol/L时,可显著缓解CGA(100μmol/L)对大豆不定根的抑制作用,使其生根率分别提高12.5%和20.0%,随着生长素施用量的进一步增加(10~50μmol/L),其生根率显著下降;大豆不定根培养24 h内,对照下胚轴基部木质素相对含量降低,CGA处理大豆的木质素相对含量升高。高浓度CGA对大豆不定根形成具有抑制作用,其通过与木质素、生长素的共同作用,实现对大豆不定根发生的调控。  相似文献   

2.
该文研究了外源绿原酸(CGA)对Al胁迫下铝敏感型黑大豆SB根生理生化指标以及根中胁迫相关基因表达的变化,探讨外源CGA缓解SB根铝毒害的效果及分子机理。以不同浓度Al和CGA处理SB,筛选出CGA缓解Al毒害的最佳浓度,测定Al含量、抗氧化系统酶活性、14-3-3蛋白与H~+-ATP酶的表达、H~+泵活性。结果表明:低浓度CGA能缓解Al胁迫下黑大豆SB根伸长抑制,并促进侧根数目增加,而高浓度CGA的缓解效果下降;0.01 g·L~(-1) CGA使Al胁迫下SB根尖Al含量与MDA含量下降,促进根系柠檬酸的分泌。RTPCR和Western Bloting分析表明0.01 g·L~(-1) CGA促进Al胁迫下SB根中14-3-3b、14-3-3m、14-3-3k和GHA2基因(质膜H~+-ATP酶)的表达,抑制MATE基因的表达。同时,0.01 g·L~(-1) CGA能促进Al胁迫下质膜H~+-ATP酶蛋白磷酸化水平以及其与14-3-3蛋白结合,且能提高质膜H~+-ATP酶和H~+泵活性。因此推测外源CGA可能通过增加侧根数,增强14-3-3蛋白和质膜H~+-ATP酶基因蛋白表达水平和互作,弥补Al胁迫下MATE表达的抑制,增加柠檬酸的分泌,增强SB对铝毒害的耐受性。  相似文献   

3.
研究绿原酸(Chlorogenic acid,CGA)对静息状态及脂多糖(Lipopolysaccharides,LPS)激活状态下小鼠腹腔巨噬细胞功能的影响。采用不同浓度的绿原酸作用于静息的和经LPS刺激的小鼠腹腔巨噬细胞,噻唑蓝(MTT)比色法检测细胞活力,中性红吞噬实验检测巨噬细胞吞噬能力,Griess法检测NO的产生,酶联免疫法(ELISA)检测巨噬细胞细胞培养上清液中IL-1β、TNF-α、IL-10的分泌水平。试验结果表明在正常状态及LPS激活状态下,绿原酸均能提高下小鼠腹腔巨噬细胞的代谢活力、增强吞噬能力、增加NO、促炎性细胞因子IL-1β、TNF-α的分泌量,降低抑炎性细胞因子IL-10的分泌量,且作用效果呈剂量依赖性。  相似文献   

4.
绿原酸药效学研究进展   总被引:7,自引:0,他引:7  
近年来,对绿原酸的研究较多,研究证明绿原酸具有广泛的药理作用,如抗氧化、抑菌、抗病毒、抗炎、抗肿瘤、保肝作用、抗内毒素等。对绿原酸的深入研究,将有利于在新药研发和临床应用中避开不良反应,增强临床疗效,为我们开发和利用绿原酸提供重要的理论依据。  相似文献   

5.
嗜铬粒蛋白(CGA)是存在于分泌细胞的由439个氨基酸组成的可溶性蛋白。近年的研究发现CGA的N端具有抗血管收缩、抗细菌和抗真菌的功能。为了寻找高效低毒的抗真菌片段,利用PCR技术扩增了编码人嗜铬粒蛋白N端1-76位氨基酸(CGA1-76)的DNA片段,并将之克隆进本实验构建的枯草杆菌诱导型表达载体pSBPTQ,获得含CGA1-76基因的重组质粒pSVTQ,转化蛋白酶三缺陷的枯草杆菌DB403。经蔗糖诱导后,CGA1-76片段在枯草杆菌DB403(pSVTQ)中获得表达,产物分泌到细胞外,分泌量约为5mg/L,占总分泌蛋白的133% 。测试了表达产物对几种丝状真菌和酵母的抑制作用,发现在4μmol/L的浓度下,枯草杆菌表达的重组CGA1-76对镰刀菌、链格孢霉及白假丝酵母有明显的抑制作用。  相似文献   

6.
杜仲细胞悬浮培养生产绿原酸的初步研究   总被引:2,自引:0,他引:2  
对影响杜仲细胞悬浮培养及其次生代谢物绿原酸产生的几种主要因子进行了研究。结果表明,在杜仲细胞悬浮培养生产绿原酸的过程中,第15天绿原酸的含量达到最大值。35g/L的蔗糖为最适碳源,MS培养基为最适悬浮培养基,pH为5.3时利于绿原酸的合成,2,4-D、NAA对绿原酸合成的促进效果不大,添加1.0mg/L的6-BA绿原酸的合成效果较好。  相似文献   

7.
建立HPLC同时测定大叶冬青叶中新绿原酸、绿原酸、隐绿原酸、咖啡酸、对羟基肉桂酸、芦丁、异槲皮苷、异绿原酸B、异绿原酸A和异绿原酸C 10种多酚类化合物的定量分析方法,研究不同产地及不同采收时期大叶冬青叶中10种多酚类化合物的含量差异及其抗氧化活性。采用HPLC测定大叶冬青叶50%甲醇提取液中10种多酚类成分的含量,并选用DPPH法对其抗氧化活性进行初步探索。结果表明,10种多酚类化合物分离效果较好,标准曲线在检测范围内具有良好的线性(r0.999 5),平均加样回收率在96.58%~112.03%,RSD4%(n=6)。大叶冬青叶50%甲醇提取液抗氧活性良好。本实验建立的方法快速、准确、重复性好,可用于同时测定大叶冬青叶中新绿原酸、绿原酸、隐绿原酸、咖啡酸、对羟基肉桂酸、芦丁、异槲皮苷、异绿原酸B、异绿原酸A、异绿原酸C 10种成分的含量;不同产地、不同采收时期大叶冬青叶中10种多酚类化合物含量存在一定差异;不同来源大叶冬青叶50%甲醇提取液抗氧化能力具有明显差异。  相似文献   

8.
向日葵籽是一种良好的蛋白质来源,但是,其多酚组份的存在限制了它们在食品工业中的应用。咖啡酸和绿原酸是向日葵籽里的主要多酚化合物,在碱性PH值时,咖啡酸引起食品产生轻度的桃红褪色,绿原酸则使食品褪变为深绿色,并且会降低蛋白质的消化率。现在研究人员可以用一种快速的、高度选择性的高效液相色谱技术来对绿原酸和咖啡酸  相似文献   

9.
对从稀悬液中采收小球藻的一种胶体气态阿夫隆(CGA)弥散新技术做了研究。CGA是直径25微米的小气泡。每个泡被包在一个表面活性剂的液壳中。利用CGA的漂浮性形成藻一泡复合体井漂浮至液面。采用阳离子表面活性剂(氯小二烷基毗咤)形成CGA时,  相似文献   

10.
嗜铬颗粒蛋白A(Chromogranin A,CGA)属于独特的酸性可溶性蛋白质家族,遍布于动物内分泌系统、神经系统和免疫系统,与其他激素和多肽共贮存、共分泌.在CGA上有许多碱性氨基酸对,成为潜在的激素原转化酶切割位点.很多切割产物已经被鉴定,其中有些具有生物学功能.近年的研究表明,在多种病理、生理状态下,CGA及其衍生片段在组织中含量升高,发挥抑制性生理功能或作为疾病诊断指标.嗜铬颗粒蛋白A的N端衍生片段在机体防御系统中的抗菌功能、在心血管系统中的抗血管收缩功能已被阐明,使其作为临床候选药物具有巨大潜力;CGA完整片段在内分泌肿瘤和神经内分泌肿瘤中含量升高,可作为内分泌肿瘤和神经内分泌肿瘤诊断指标,但其在肿瘤发生过程中的作用还未见报道.  相似文献   

11.
Chlorogenic acid, a polyphenol found in coffee, has antihypertensive actions, but epidemiologic data on the effects of coffee on blood pressure are controversial. Specific coffee components that inhibit the hypotensive effect of chlorogenic acid and the physiologic mechanisms underlying the effects of coffee without these components were investigated. One component, hydroxyhydroquinone (HHQ), inhibited the hypotensive effects of chlorogenic acid in spontaneously hypertensive rats (SHR). The attenuation of hypertension by HHQ-free coffee was associated with nitric oxide, the suppression of mRNA expression of NAD(P)H oxidase, and the improvement in endothelium-dependent vasodilation in the aorta. Thus, HHQ-free coffee might regulate vascular tone by improving the bioavailability of nitric oxide in SHR.  相似文献   

12.
Chlorogenic acid prevented a stepwise conversion of plasmid pUC18 DNA, from I-->form II-->form III, induced by 3 mM monochloramine with a half inhibition of 67.4 microM. Chlorogenic acid reacted with monochloramine in a time-dependent manner, and the reaction rate increased with decreasing pH. These results suggest that chlorogenic acid prevents genotoxicity of monochloramine in gastric mucosa.  相似文献   

13.
Chlorogenic acid, a phenolic compound found ubiquitously in plants, is an in vitro antioxidant and metal chelator. Some derivatives of chlorogenic acid are hypoglycemic agents and may affect lipid metabolism. Concentrations of cholesterol and triacylglycerols are of interest due to their association with diseases such as non-insulin-dependent-diabetes- mellitus and obese insulin resistance. As little is known about the effects of chlorogenic acid in vivo, studies using obese, hyperlipidemic, and insulin resistant (fa/fa) Zucker rats were conducted to test the effect of chlorogenic acid on fasting plasma glucose, plasma and liver triacylglycerols and cholesterol concentrations. Aditionally, the effects of chlorogenic acid on selected mineral concentrations in plasma, spleen, and liver were determined. Rats were implanted with jugular vein catheters. Chlorogenic acid was infused (5 mg/Kg body weight/day) for 3 weeks via intravenous infusion. Chlorogenic acid did not promote sustained hypoglycemia and significantly lowered the postprandial peak response to a glucose challenge when compared to the same group of rats before Chlorogenic acid treatment. In Chlorogenic acid-treated rats, fasting plasma cholesterol and triacylglycerols concentrations significantly decreased by 44% and 58% respectively, as did in liver triacylglycerols concentrations (24%). We did not find differences (p > 0.05) in adipose triacylglycerols concentration. Significant differences (p < 0.05) in the plasma, liver, and spleen concentration of selected minerals were found in chlorogenic acid-treated rats. In vivo, chlorogenic acid was found to improve glucose tolerance, decreased some plasma and liver lipids, and improve mineral pool distribution under the conditions of this study.  相似文献   

14.
Previous studies indicated that chlorogenic acid, a compound present in many fruits and vegetables, has anti-cancer activities. We report that chlorogenic acid regulates the expression of apoptosis-related genes and self-renewal-related stem cell markers in cancer cells. The lung cancer cell line A549 was cultured with or without chlorogenic acid. The presence of chlorogenic acid decreased cell proliferation as measured by MTT activity. Polymerase chain reaction (PCR) showed that treatment of cells with chlorogenic acid reduced the expression of BCL2 but increased that of both BAX and CASP3. Chlorogenic acid enhanced annexin V expression as measured using fluorescently labeled annexin V. Chlorogenic acid also induced p38 MAPK and JNK gene expression. Meanwhile, several agents, including SB203580 (p38 MAP kinase inhibitor), N-acetylcysteine (antioxidant inhibitor), dipyridamole (phosphodiesterase inhibitor), and apocynin (NADPH-oxidase inhibitor) blocked chlorogenic acid-induced BAX gene expression. Chlorogenic acid reduced gene expression levels of stem cell-associated markers NANOG, POU5F1, and SOX2. Together these results indicate that chlorogenic acid affects the expression of apoptosis-related genes that are part of oxidative stress and p38 MAP-dependent pathways, as well as genes encoding stem cell markers. In conclusion, chlorogenic acid may contribute to the polyphenolic anti-cancer effect associated with consumption of vegetables and fruits.  相似文献   

15.
Chlorogenic acid is the major polyphenol in foods derived from plants and is a good substrate for polyphenol oxidase. Chlorogenic acid quinone (CQA-Q), which is an oxidative product of chlorogenic acid by polyphenol oxidase, is an important intermediate compound in enzymatic browning. CQA-Q was prepared, and its properties and the relationship with browning were examined. The quinone solution was yellow or orange, and its molecular absorption coefficient was estimated to be 1.7 x 10(3) for 325 nm and 9.7 x 10(2) for 400 nm in an acidic aqueous solution. Chlorogenic acid and H2O2 were spontaneously generated in the CQA-Q solution as the yellowish color of the solution gradually faded. A pale colored polymer was the major product in the reaction solution. Amino acids such as lysine and arginine added to CQA-Q solution did not repress the fading of the yellowish color of the solution. We concluded from these results that CQA-Q itself and a mixture of CQA-Q and amino acids did not form intensive brown pigments in the acidic aqueous solution. H2O2 spontaneously formed in the CQA-Q solution, and other polyphenols might have played an important role in the formation of the brown color by enzymatic browning.  相似文献   

16.
Mc Clure , T. T. (Plant Pest Control Division, ARS, USDA, Washington, D. C.) Chlorogenic acid accumulation and wound healing in sweet potato roots . Amer. Jour. Bot. 47(4) : 277—280. Illus. 1960.–Chlorogenic acid accumulation in cells adjacent to a wound occurs before suberization and wound-periderm formation. Suberization during wound healing was highly correlated with chlorogenic acid accumulation and with wound-periderm formation. The possible role of chlorogenic acid as a source of chemical units for suberization is suggested. Histochemical tests indicate that suberization during wound healing may be a form of lignification. Over 5 times as much lignin was found by chemical analysis in the tissues of healed surfaces as in controls.  相似文献   

17.
柯玉琴  潘廷国 《应用生态学报》2002,13(10):1303-1306
随NaCl胁迫浓度的提高 ,甘薯苗期株高和叶面积生长受抑制 ;叶片IAA水平下降 ,而IAA氧化酶和POD活性提高 .不耐盐品种生长受抑制程度及IAA水平下降幅度均大于中耐盐品种和耐盐品种 ,叶片IAA氧化酶和POD活性上升的幅度大于中耐盐及耐盐品种 .同时浓度NaCl胁迫还引起了叶片绿原酸含量的增加 ,中耐盐品种栗子香和不耐盐品种胜利百号在 170mmol·L-1的NaCl浓度胁迫下 ,绿原酸含量上升达到最高值 ,耐盐品种徐薯 18绿原酸含量达到最高值的浓度是 2 5 5mmol·L-1,而后随NaCl胁迫浓度的提高 ,绿原酸含量均有下降 ,但仍高于对照 .  相似文献   

18.
Phytochemistry Reviews - Chlorogenic acids (CQAs), the esters of caffeic and quinic acid, are biologically important phenolic compounds present in many plant species. Nowadays the knowledge about...  相似文献   

19.
杜仲茶加工工艺与营养成分相关性研究   总被引:3,自引:0,他引:3  
本文利用高效液相色谱仪、氨基酸自动分析仪等对不同加工工艺所生产的杜仲茶中的绿原酸、氨基酸、水溶性糖及蛋白质的含量进行了测定。结果表明:采用炒青的加工工艺所得的杜仲茶质量最佳。  相似文献   

20.
Chlorogenic acid is the major polyphenol in foods derived from plants and is a good substrate for polyphenol oxidase. Chlorogenic acid quinone (CQA-Q), which is an oxidative product of chlorogenic acid by polyphenol oxidase, is an important intermediate compound in enzymatic browning. CQA-Q was prepared, and its properties and the relationship with browning were examined. The quinone solution was yellow or orange, and its molecular absorption coefficient was estimated to be 1.7×103 for 325 nm and 9.7×102 for 400 nm in an acidic aqueous solution. Chlorogenic acid and H2O2 were spontaneously generated in the CQA-Q solution as the yellowish color of the solution gradually faded. A pale colored polymer was the major product in the reaction solution. Amino acids such as lysine and arginine added to CQA-Q solution did not repress the fading of the yellowish color of the solution. We concluded from these results that CQA-Q itself and a mixture of CQA-Q and amino acids did not form intensive brown pigments in the acidic aqueous solution. H2O2 spontaneously formed in the CQA-Q solution, and other polyphenols might have played an important role in the formation of the brown color by enzymatic browning.  相似文献   

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