Acute effect of cold on the antioxidant enzymes activities and uncoupling protein-1 content in the brown fat of 6-hydroxydopamine-treated rats

1. Uncoupling protein-1 (UCP-1) content and activity of antioxidant enzymes, copper–zinc superoxide dismutase (CuZnSOD), manganese superoxide dismutase (MnSOD) and catalase (CAT), as well as that of the monoamine oxidase (MAO) in the interscapular brown adipose tissue (IBAT) of 6-hydroxydopamine (6-HDA)-treated rats at 22 °C or cold (4 °C, 4 h), were studied.

2. Results indicate that the intact sympathetic nerves (SN) are necessary for the maintenance of basal level of IBAT UCP-1 t and SODs, but not for MAO. They also suggest that in the regulation of IBAT UCP-1 content, in 6-HAD-treated rats exposed to cold, in which this was normalized, and other mechanisms rather than SN are involved.

Effect of heat and cold exposure on the rat brain monoamine oxidase and antioxidative enzyme activities

1. Changes in MAO and antioxidative enzymes copper-zinc superoxide dismutase (CuZnSOD), manganese superoxide dismutase (MnSOD) and catalase (CAT) activities were examined in the hypothalamus and the hippocampus of Wistar rats exposed to cold stress (6 °C) for 180 min and heat stress (38 °C) for 60 min.

2. Extreme environmental temperatures caused stressor-specific changes in the hypothalamic and hippocampal MAO and antioxidative enzyme activities, being dependent on the stressor applied (cold or heat) but not on the brain region studied (the hypothalamus or hippocampus).

Thermal acclimation, hydroperoxide detoxifying enzymes and oxidative stress in the lung and liver of Rana perezi

1. 1.Rana perezi adult frogs were acclimated to cold (10 ± 2°C) and warm (29 ± 1°C) temperatures for 4 months.

2. 2.After acclimation, a partial compensation of the oxygen consumption of the animals was found because of a reduction of its thermal sensitivity.

3. 3.Activities of liver and lung catalase, selenium (Se)-dependent and Se-independent glutathione peroxidases were not changed by thermal acclimation.

4. 4.Tissue peroxidation (TBA-RS) increased in the liver of heat acclimated animals.

5. 5.Hydroperoxide detoxifying enzyme activities did not show inverse compensation of temperature during acclimation. It is proposed that the pattern of thermal compensation shown by these enzymes in different species depends on a variety of factors including: (a) the thermal sensitivities of hydroperoxide producing and scavenging systems; (b) the changes induced by acclimation in the rate of hydroperoxide generation.

Stereological analysis of mitochondria from brains of temperature acclimated goldfish, Carassius auratus L. (5 and 30°C)

1. 1.|The mitochondrial population in hypothalamic and hypophysial brain tissue from warm (30°C) and cold (5°C) acclimated goldfish (Carassius auralus L.) was analyzed using sterological techniques.

2. 2.|It was revealed that there is a significantly larger volume density (Vv) in the cold acclimated tissue, with no significant difference in either of the surface densities (Svext and Svint) from either of the brain areas.

3. 3.|The hypothalamic brain tissue has a significantly lower specific surface (S/V) in the cold acclimated tissue but there is not a significant difference in this parameter for the hypophysial brain tissue.

4. 4.|The values for these three parameters (Vv, Svext and SVint, and S/V) indicate that mitochondria from acclimated brain tissue undergo shape changes in response to thermal stress.

5. 5.|We suggest that the shape changes may be related to the change in the phospholipid composition of the inner mitochondrial membrane with acclimation temperature.

Biochemical changes in tissue composition of Periplaneta americana (Linn.) acclimated to high and low temperatures

1. 1.|Cold acclimation apparently favours an increase of water content in fat body, but not in coxal muscle, of cockroaches.

2. 2.|A remarkable enhancement in the accumulation of total protein in fat body characterizes the cold acclimation of cockroaches, particularly adult males (175% increase in protein/DNA ratio). The increase in protein content of coxal muscle during acclimination to 15°C, observed in nymphs (16%) and males (16%) but not in females, is less pronounced than that of fat body.

3. 3.|A diminution (28–32%) in the free amino acid/DNA ratio due to cold acclimation has been recorded in both coxal muscle and fat body of nymphs and females, but not in males.

4. 4.|No qualitative change occurs in the free amino acid spectrum of haemolymph and tissues of this insect during acclimation to 15 and 35°C.

5. 5.|An augmentation (15–30%) of the RNA/DNA ratio occurs in fat body and coxal muscle of nymphs and males but in fat body alone of females following cold acclimation.

6. 6.|The glycogen reserve has been shown to increase by up to 30% in fat body and coxal muscle of cold acclimated cockroaches compared to warm acclimated ones.

The effect of direct and indirect hand heating on finger blood flow and dexterity during cold exposure

1. The aim of this study was to investigate if finger temperature or finger blood flow is the critical factor for maintenance of finger dexterity during cold exposure.

2. Subjects were exposed twice to −25°C air for 3 h by using a Torso Heating Test (THT) where the torso was maintained to 42°C with a heating vest while the hands were bare, and a Hand Heating Test (HHT) where the hands were heated with heated gloves.

3. Despite similar finger temperatures, finger blood flow was eight times lower and finger dexterity was decreased in HHT as compared to THT.

4. It is concluded that finger blood flow is the critical factor to maintain finger dexterity in the cold.

The effect of hypothermia on the cardiovascular system and the pressor actions of angiotensin II

1. 1.|The effect of hypothermia (24°C) on the pressor action of angiotensin II (ANG II) was studied in anaesthetized rats.

2. 2.|Hypothermia prolonged the pressor response to ANG II leading to an increase in the estimated half-life of ANG II.

3. 3.|Hypothermia also caused a significant increase in stroke volume and a significant decrease in heart rate with no change in cardiac output.

4. 4.|It is conclued that hypothermia causes a prolongation of the pressor action of ANG II probably by reducing the activity of the catabolic enzymes leading to an increase in ANG II half-life.

The effect of acclimation temperature and the removal of peripheral temperature receptors on body temperature preference in the cockroach, Periplaneta americana

1. 1.|Body temperature preferences were compared between cockroaches acclimated to different ambient temperatures and between 25°C acclimated cockroaches and cockroaches deprived of their peripheral temperature receptors.

2. 2.|Acclimation to 35°C resulted in a significantly higher mean body temperature and low body temperature selected compared with 25°C acclimated cockroaches.

3. 3.|Cockroaches deprived of their peripheral temperature receptors showed a significantly higher mean high body temperature selected when compared to normal 25°C acclimated cockroaches.

4. 4.|It is concluded that cockroach temperature regulation is more precise than expected and that central temperature receptors are the primary sensing elements for cockroach thermoregulation.

Compensation limits of fish muscle myofibrillar ATPase enzyme to environmental temperature

1. 1.|Goldfish acclimated to a range of temperatures between 5 and 35°C were found to only compensate the specific activity of their myofibrillar ATPase enzyme between 10 and 30°C.

2. 2.|The preferred temperatures of goldfish acclimated to 5°C and to 30°C were determined to be about 10 and 26°C respectively.

3. 3.|It is conlcuded that goldfish are only able to acclimate their myofibrillar ATPase system to temperatures between 10 and 30°C, but acclimation to these temperatures enables them to tolerate extremes.

Effects of fasting on heat balance and nonshivering thermogenesis in febrile adult guinea pigs

1. 1. The calorigenic responses of 60-h fasted (F) and control (C) guinea pigs to E. coli endotoxin (5 μg·kg⁻¹ i.v.) injection were compared at T_a = 25°C.

2. 2. In contrast to fed (C) guinea pigs, the F group showed significantly lower calorigenic response.

3. 3. The brown adipose tissue (BAT) thermogenesis judge by the BAT temperature did not change significantly. Shivering activity was not different from that in the C group.

4. 4. The results conclude that fasting attenuates endotoxin-induced fever and this attenuation is due to suppression of nonshivering thermogenesis (NST) in guinea pigs.

5. 5. The results from direct calorimetry indicate that endotoxin injection evoked a prominent increase in heat production, while the changes in heat loss do not have an important role.

Work at cold storage

1. 1. Work activities in cold storage rooms were assessed by a mailed questionnaire survey of cold storage facilities in Japan.

2. 2. There are nearly 4000 cold storage facilities and about 80% are being kept at temperatures below −20°C.

3. 3. The chief items of stock in storage were marine products, livestock products, frozen food and agricultural products.

4. 4. Methods used for loading and unloading in cold storage rooms are forklift, manual handling, and automatic machines.

5. 5. Use of forklifts appeared to be widespread.

6. 6. Working time differed according to the ambient temperature of the cold storage rooms.

7. 7. Common ailments of cold storage workers are lumbago, bronchitis, neuralgia etc.

The effect of acclimation temperature on energy metabolism as a function of body weight in rats

1. 1|Energy metabolism of 38 rats acclimated to 10, 20 and 30°C was measured at these ambient temperatures and the regression equations over body weight were calculated.

2. 2|Expressing metabolic rate as M = kWⁿ, the results showed that the value of n is apparently variable according to the acclimation and measurement temperatures.

3. 3|It was also shown that the 2/3 power of body weight is a suitable parameter to express metabolic rate, regardless of acclimation or environmental temperatures.

Comparison of thermal responses with and without cold protective clothing in a warm environment after severe cold exposures

1. 1. Ten male students remained in a severely cold room (-25°C) for 20 min. thereafter, they transferred in a warm room (25°C) for 20 min.

2. 2. This pattern was repeated three times, total cold exposure time amounting to 60 min.

3. 3. In the warm room, the subjects removed their cold-protective jackets, or wore them continously.

4. 4. Rectal temperature, skin temperatures, manual performance and thermal comfort were measured during the experiment.

5. 5. Removing cold-protective jackets after severe cold exposure increased peripheral skin temperatures and reduced the discomfort in the warm room.

6. 6. However, these results were accompanied by a greater decrease in rectal temperature and manual performance.

7. 7. It is recommended that workers continue to wear cold-protective clothing in the warm areas outside of the cold storage to prevent decreases in deep body temperature and work efficiency caused by repated cold exposures.

Thermal acclimation and hardening in tadpoles of the bullfrog, Rana catesbeiana

1. 1.|Critical thermal maxima (CTMax) and minima (CTMin) were measured to evalute thermal hardening in Rana catesbeiana.

2. 2.|Tadpoles show heat hardening and CTMax acclimation, and both responses are influenced by developmental stage.

3. 3.|The first evidence of cold hardening in vertebrates is reported here.

4. 4.|Heat hardening significantly reduces cold tolerance, but there is otherwise no evidence of a cross-hardening effect.

The effect of adaptation to cold and re-adaptation to room temperature on the level of glutathione in rat tissues

1. Glutathione (GSH) was assayed in plasma, liver and IBAT in control (22±1°C) and cold adapted rats (45 days in 5±1°C), and in rats cold adapted and brought back to room temperature after 1, 3, 7 and 15 days.

2. Adaptation to the cold led to reduced GSH in the liver and plasma while the level in intrascapular brown adipose tissue (IBAT) was increased in comparison to controls.

3. On the first day of re-adaptation, plasma GSH was similar to the control level, as were hepatic levels on the first and fifteenth day, but GSH in IBAT remained higher even after fifteen days.

Effect of propranolol and cold exposure on the activities of antioxidant enzymes in the brain of rats adapted to different ambient temperatures

The exposure to cold (6 h; 6°C) induced a significant decrease in both hypothalamic and brain stem CuZn-superoxide dismutase as well as an increase in Mn-superoxide dismutase and catalase activities in Wistar male rats, acclimated to 6±1°C as compared to those acclimated to 22±2°C. If the rats were administered with propranolol (15 mg/kg), which is a β-adrenoceptor blocker, there were no significant differences in the enzyme activities in any of the brain regions of the two groups studied. It was concluded that acute exposure to cold induces changes in the hypothalamic and brain stem antioxidant enzyme activities dependent on the previous acclimation to different ambient temperatures and propranolol administration.     

A relationship between heat load and plasma enzyme concentration

1. 1. The sensitivity of serum enzyme levels as indicators of tissue damage is less well established in the prodromal period of heatstroke, especially for sub-lethal stress conditions.

2. 2. Anaesthetized rats were exposed to two different sets of thermal conditions.

3. 3. Plasma levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), creatine kinase (CK) and lactate dehydrogenase (LD) were assayed in each group upon termination of stress, 6 h post-stress and 24 h post-stress.

4. 4. The tissue “damage” sustained was mild to moderate and completely reversible.

5. 5. The rate of rise in body temperature may constitute an important factor in the ultimate pathology.

6. 6. CK proved to be the most sensitive parameter of tissue “damage”.

TLV in cold environments

1. 1. The risks encountered during cold exposure are general body cooling or local cooling of parts of th body.

2. 2. Measures of cold stress must account for the effects of climate, clothing and metabolic heat production on heat balance.

3. 3. The combinaed effect of air temperature, mean radiant temperature, humidity and air velocity determines the cooling power of the environment.

4. 4. The cooling power can be easily converted into a required insulation value (IREQ) for whole body heat balance.

5. 5. Extensive cooling of hands and feet may be a limiting factor, even when sufficient total insulation is provided. In addition the cooling effect of wind on unprotected skin must be considered.

6. 6. Recommendation regarding acceptable exposures can be expressed as lowest ambient temperatures and time limits as function of available protection and activity level, with due attention to both general and local effects.

Preferred temperature of the elderly after cold and heat exposures determined by individual self-selection of air temperature

1. 1. The purpose of the study was to investigate the preferred temperature of the elderly after cold and heat exposures.

2. 2. Eight elderly and 9 young females wearing the same type of clothing were exposed to cold (10°C), moderate (25°C) or hot (35°C) environments for 30 min in the exposure room.

3. 3. Then they moved to the self-control room in which the temperature was set at 25°C, and the room temperature increased or decreased continuously by 0.4°C every minute.

4. 4. The subjects were instructed to operate the switch when they felt uncomfortably warm or cool during a 90-min period.

5. 5. In operating the switch, the changing in room temperature shifted to the opposite direction.

6. 6. The ambient temperature was recorded continuously and analyzed as the preferred temperature, which was defined as the midpoint temperature of the crest and trough of temperature records.

7. 7. The preferred temperatures after the cold exposure were significantly higher than those of other exposure conditions in the elderly.

8. 8. On the other hand, in the young, there was no significant difference in the preferred temperature among the exposure conditions.

9. 9. Although the effect of exposure to cold or hot environments decreased in the latter parts of self-control, the elderly still preferred the higher temperature after cold exposure.

Central thermosensitivity and the integrative responses of hypothalamic neurons

1. Hypothalamic thermosensitive neurons are important in the integrative regulation of body temperature. In addition to receiving afferent thermal input, their activity correlates with the stimulation of thermoregulatory mechanisms.

2. While cold sensitivity is synaptically mediated, warm sensitivity depends on inherent cellular responses. Warm sensitive neurons may also respond to other homeostatic variations, hormones, and endogenous mediators.

3. In contrast to peripheral thermoresponsiveness, which depends on conductance changes that regulate membrane potential, hypothalamic warm sensitivity relies on regulating the prepotential phase of the action potential.

4. Additionally, the recent morphologic characterization of these neurons supports the criterion for warm sensitivity.