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1.
Gerardo Montelongo-Ruíz Yolanda del Rocio Moreno-Ramírez María Cruz Juárez-Aragón Nohemí Niño-García Reyna Ivonne Torres-Acosta Torres-Castillo Jorge Ariel 《Phyton》2020,89(2):361-373
This work aims to determine the phytochemical characterization of the
pericarp of Chamaedorea radicalis Mart. fruit as a non-timber product with
potential to obtain phytochemicals with potential applications in the industry.
Fruit from C. radicalis were grouped in four ripening stages named as S1, S2,
S3 and S4, according to maturity; S1 the most unripe stage and S4 the completely
ripe stage. Determinations of total phenolic compounds, free radical scavenging
activities and total flavonoid contents using spectrophotometric methods were
done. Also, the tentative identification of phytochemicals during fruit ripening
was done using UPLC-MS-MS. Total phenolic compound (TPC) content ranged
from 7.24 to 12.53 mg gallic acid equivalents per gram of fresh weight (mg GAE/
g FW). Total flavonoids (TF) contents ranged from 0.163 to 0.23 mg of quercetin
equivalents per g FW (mg QE/g FW). Free radical scavenging activity against
DPPH and ABTS radicals varied from 40.80 to 53.68 and from 22.29 to
37.76 mmol Trolox equivalents g FW (mmol TE/g FW), respectively. Antioxidant assay in vitro by FRAP (ferric reducing antioxidant power) method showed
that S3 was the highest level with antioxidant power while S4 was the lowest
with Red ripeness stage showed the lowest contents for all determinations. Mass
spectrometry allowed detection of 26 compounds, including phenolics, alkaloids
and saponins. Afzelin, Kaempferol 3-neohesperidoside and the four saponins
identified were present in all ripeness stages. Preliminary phytochemical identi-
fication and the spectrophotometric determinations showed that the pericarp of
C. radicalis presented antioxidants and compounds related to alkaloids, phenolics and saponins. The presence and abundance of each phytochemical regarding
each ripeness stage should be considered. 相似文献
2.
Kuntal Das Syed Mohammed Basheeruddin Asdaq M. Saifulla Khan Sravani Singirikonda Abdulhakeem S. Alamri Walaa F. Alsanie Majid Alhomrani Sreeharsha Nagaraja Katharigatta N. Venugopala 《Phyton》2022,91(10):2349-2365
The current study used RP-HPLC to compare phytochemicals and estimate the bioactive constituents found in Stevia rebaudiana Bert. (SRB) leaves collected from two different geographical sources. SRB leaves were collected from Bangalore, Karnataka, India, and Reduit, Mauritius. Extracts were prepared using ethanol and aqueous solvents. Proximate analysis was used to evaluate moisture content, ash values, crude fibers, and extractive values. Following that, preliminary phytochemical screening was done on both ethanol leaves extracts, and subsequently total flavonoid content was determined. In addition, TLC chromatograms and RP-HPLC studies were performed on both plant extracts to determine the presence of flavonoid components in both leaves extracts, followed by in vitro anti-diabetic activity was performed with alpha amylase and alpha glucosidase enzymes against Acarbose as standard. Results revealed that both the extracts from two different geographical sources varied significantly with the yield, content of chemicals, and presence of quercetin (flavonoid) content when estimated through the RP-HPLC standardized method. Glycosides, flavonoids, proteins, steroids, saponins, terpenoids, and phenols were found in various concentrations during phytochemical screening. Among both zones, the ethanol leaves extract of SRB taken from Mauritius had a greater content of phytochemicals and a higher yield than other extracts due to the soil nature. The Mauritius sample had greater total flavonoid levels as well as more quercetin (0.92 ± 0.011) than the other extracts. Following that, ethanol extract inhibited enzymes (alpha amylase, alpha glucosidases) more than aqueous extract, and this inhibition was dose dependent. Among them, the Mauritius ethanol sample showed higher anti-diabetic efficacy than the Indian sample, but this difference was not significant. Overall, SRB ethanol leaves extracts outperformed other leaves extracts in terms of yield, phytoconstituents, and total flavonoids. Overall, both SRB samples had high quercetin levels and possessed anti-diabetic potential, but they were greater in the Mauritius sample, demonstrating that plant traits are influenced by geographic location. 相似文献
3.
Kosteletzkya virginica (K. virginica) is used for revegetation of salt-affected coastal tidal flats and as a raw material
of biodiesel. K. virginica root tuber, a biowaste with low economic value, is rich in bioactive compounds. This
study aimed to extract and identify flavonoids from K. virginica root tubers. The optimal extraction conditions
were 1/25 (w/v) solid/liquid ratio, 40% ethanol concentration at 40°C for 60 min. Under these conditions,
65.2 ± 3.7 mg/g total flavonoid content was extracted from the roots, which were collected from salinized soil
in late autumn of the third year. Antioxidant activity was evaluated through 1,1-diphenyl-2-picrylhydrazyl,
hydroxyl radical, and superoxide anion scavenging assays. The extracted flavonoids exhibited antioxidant activity
in a dose-dependent manner. Five flavonoids, glucoliquiritin apioside, licoisoflavone B, 5-methoxy-7,8-diprenyl-
flavone, 7,2′-dihydroxy-6,8-dimethyl-4′,5′-methylenedioxyflavan, and 5,7,4′-trihydroxy-3′-methoxy-6,8-di-Cmethylflavanone, were identified by ultra-performance liquid chromatography–tandem mass spectrometry.
Our results suggest that the flavonoids of K. virginica root tubers might be potent antioxidants and can be effectively applied as an ingredient in food and natural medicine. 相似文献
4.
Brassica rapa var. rapa (turnip) is an important crop in Qinghai-Tibet
Plateau (QTP) with anti-hypoxic effect. Turnip is rich in glucosinolates,
isothiocyanates and phenolic compounds with diverse biological activities,
involving anti-oxidant, anti-tumor, anti-diabetic, anti-inflammatory, anti-microbial,
hypolipidemic, cardioprotective, hepatoprotective, nephroprotective and analgesic
properties. In this study, the ethyl acetate (EtOAc) and butanol parts of Brassica
rapa were first revealed with inhibitory effects on α-glucosidase, whereas the water
part was inactive. Subsequent bioassay-guided isolation on the EtOAc and butanol
parts yielded 12 compounds, involving three indole derivatives, indole-3-
acetonitrile (1) 4-methoxyindole-3-acetonitrile (2) and indole-3-aldehyde (3) two
flavonoids, liquiritin (4) and licochalcone A (5) two phenylpropanoids, sinapic acid
(6) and caffeic acid (7) two phenylethanol glycosides, 2-phenylethyl β-
glucopyranoside (8) and salidroside (9) and three other compounds, syringic acid
(10) adenosine (11) and (3β, 20E)-ergosta-5, 20 (22)-dien-3-ol (12) Licochalcone
A (5) and caffeic acid (7) showed α-glucosidase inhibitory activity with IC50 values
of 62.4 ± 8.0 μM and 162.6 ± 3.2 μM, comparable to the positive control, acarbose
(IC50 = 142 ± 0.02 μM). Docking study suggested that licochalcone A (5) could
well align in the active site of α-glucosidase (docking score = -52.88) by forming
hydrogen bonds (Gln1372, Asp1420, Gln1372, Arg1510), hydrophobic effects
(Tyr1251, Tyr1251, Trp1355, Phe1560, Ile1587, Trp1355, Phe1559, Phe1559) and
π-π stacking interaction (Trp1355). This study provides valuable information for
turnip as a new resource in searching anti-diabetic candidates. 相似文献
5.
Houda Bouslimi Renata Ferreira Nesrine Dridi Pedro Brito Susete Martins-Dias Isabel Caçador Noomene Sleimi 《Phyton》2021,90(1):145-158
Soil contamination by toxic trace metal elements, like barium (Ba), may stimulate various undesirable changes in the metabolic activity of plants. The plant responses are fast and with, direct or indirect, generation of reactive oxygen species (ROS). To cope with the stress imposed by the ROS production, plants developed a dual cellular system composed of enzymatic and non-enzymatic players that convert ROS, and their by-products, into stable nontoxic molecules. To assess the Ba stress response of two Brassicaceae species (Brassica juncea, a glycophyte, and Cakile maritime, a halophyte), plants were exposure to different Ba concentrations (0, 100, 200, 300 and 500 μM). The plants response was evaluated through their morphology and development, the determination of plant leaves antioxidant enzymatic activities and by the production of plants secondary metabolites. Results indicated that the two Brassicaceae species have the ability to survive in an environment containing Ba (even at 500 μM). The biomass production of C. maritima was slightly affected whereas an increase in biomass B. juncea was noticed. The stress imposed by Ba activated the antioxidant defense system in the two species, noticed by the changes in the leaves activity of catalase (CAT), ascorbate peroxidase (APX) and guaicol peroxidase (GPX), and of the secondary metabolites, through the production of total phenols and flavonoids. The enzymatic response was not similar within the two plant species: CAT and APX seem to have a more important role against the oxidative stress in C. maritima while in B. juncea is GPX. Overall, total phenols and flavonoids production was more significant in the plants aerial part than in the roots, of the both species. Although the two Brassicaceae species response was different, in both plants catalytic and non-catalytic transformation of ROS occurs, and both were able to overcome the Ba toxicity and prevent the cell damage. 相似文献
6.
Oliver Tusevski Marija Todorovska Jasmina Petreska Stanoeva Marina Stefova Sonja Gadzovska Simic 《Phyton》2023,92(3):921-941
Three Hypericum perforatum hairy root lines (HR B, HR F and HR H) along with non-transformed roots were analyzed for phenolic compounds composition and in vitro enzyme inhibitory properties. In silico molecular modeling was performed to predict the interactions of the most representative phenolic compounds in HR clones with enzymes related to depression, neurodegeneration and diabetes. Chromatographic analyses revealed that HR clones represent an efficient source of quinic acid and hydroxybenzoic acids, epicatechin and procyanidin derivatives, quercetin and kaempferol glycosides, as well numerous xanthones. In vitro antidepressant activity of HR extracts through monoamine oxidase A (MAO-A) inhibition was attributed to the production of oxygenated and prenylated xanthones. The neuroprotective potential of HR extracts was related to the accumulation of quercetin 6-C-glucoside, epicatechin, procyanidins and γ-mangostin isomers as potential inhibitors of acetylcholinesterase (AChE) and butyrylcholinesterase (BChE). Vanillic acid and prenylated xanthones in HR clones as promising inhibitors of tyrosinase additionally contributed to the neuroprotective activity. Five preeminent xanthones in HR (γ-mangostin, mangiferin, garcinone C, garcinone E and 1,3,7-trihydroxy-6-metoxy-8-prenyl xanthone) along with the flavonol quercetin 6-C-glucoside effectively inhibited α-amylase and α-glucosidase indicating the antidiabetic properties of HR extracts. Transgenic roots of H. perforatum can be exploited for the preparation of novel phytoproducts with multi-biological activities. 相似文献
7.
Ljiljana Janjušević Filip Šibul Giuseppina Tommonaro Carmine Iodice Dragica Jakovljević 《Journal of enzyme inhibition and medicinal chemistry》2017,32(1):355-362
This study aimed to determine antiradical (DPPH? and ?OH) and acetylcholinesterase (AChE) inhibitory activities along with chemical composition of autochtonous fungal species Trametes versicolor (Serbia). A total of 38 phenolic compounds with notable presence of phenolic acids were identified using HPLC/MS-MS. Its water extract exhibited the highest antiradical activity against ?OH (3.21?μg/mL), among the rest due to the presence of gallic, p-coumaric and caffeic acids. At the concentration of 100?μg/mL, the same extract displayed a profound AChE inhibitory activity (60.53%) in liquid, compared to donepezil (89.05%), a drug in clinical practice used as positive control. The flavonoids baicalein and quercetin may be responsible compounds for the AChE inhibitory activity observed. These findings have demonstrated considerable potential of T. versicolor water extract as a natural source of antioxidant(s) and/or AChE inhibitor(s) to be eventually used as drug-like compounds or food supplements in the treatment of Alzheimer’s disease. 相似文献
8.
Zahid Khorshid Abbas Shalini Saggu Mohamed I. Sakeran Nahla Zidan Hasibur Rehman Abid A. Ansari 《Saudi Journal of Biological Sciences》2015,22(3):322-326
The phytochemical, antioxidant and mineral composition of hydroalcoholic extract of leaves of Cichorium intybus L., was determined. The leaves were found to possess comparatively higher values of total flavonoids, total phenolic acids. The phytochemical screening confirmed the presence of tannins, saponins, flavonoids, in the leaves of the plant. The leaf extract was found to show comparatively low value of IC50 for 2,2-diphenyl-1-picrylhydrazyl (DPPH) inhibition. The IC50 value of chicory leaves extract was found to be 67.2 ± 2.6 μg/ml. The extracts were found to contain high amount of mineral elements especially Mg and Zn. Due to good phytochemical and antioxidant composition, C. intybus L., leaves would be an important candidate in pharmaceutical formulations and play an important role in improving the human health by participating in the antioxidant defense system against free radical generation. 相似文献
9.
Forsythiae Fructus, the fruit of Forsythia suspense is a traditional Chinese hebal medicine that has the antiviral
and antioxidant effects in China. Modern analytical chemistry studies showed that the extracts of Forsythiae Fructus contain many bioactive components, such as flavonoids, lignans, phenolic acids, and terpenoids, which can be
used to anti-inflammatory and treat toxicity, tonsillitis, ulcers, pharyngitis and acute nephritis. In order to study
the types and quantities of metabolites in Forsythiae Fructus, we isolated, identified and analysed metabolites
between two varieties of Forsythiae Fructus using UPLC/ESI-Q TRAP-MS/MS. The results showed that a total
of 407 metabolites were identified in Forsythiae Fructus using UPLC/ESI-Q TRAP-MS/MS, including 21 terpenoids, 68 phenolic acids, 63 flavonoids, 43 amino acids and derivatives, 22 alkaloids, 55 lipids, 24 lignans and
coumarins, 31 nucleotides and derivatives, 29 organic acids, and 51 other metabolites. Among, lignans and coumarins, terpenoids, organic acids, lipids, and phenolic acids were rich in Forsythiae Fructus, which accounted for
more than 60% of the total metabolite content. Differential metabolite analysis revealed that 80 metabolites differed significantly between the two types of Forsythiae Fructus. Our results greatly enrich the Forsythiae Fructus
phytochemical composition database and provide valuable information for further study of the metabolites of
Forsythiae Fructus. 相似文献
10.
Aleksandar
. Kosti Uro M. Gai Mirjana B. Pei Sladjana P. Stanojevi Miroljub B. Bara Marina P. Ma
ukanovi‐Joci Stevan N. Avramov
ivoslav Lj. Tei 《化学与生物多样性》2019,16(3)
This study was aimed at investigating the phytochemical composition and antioxidant capacity of rhizomes, above‐ground vegetative parts and flowers of three Iris species: Iris humilis Georgi , Iris pumila L. and Iris variegata L. UHPLC‐Orbitrap MS analysis was used for determination of phytochemical profile. Total pigments, phenolics, flavonoids, soluble sugars and starch content as well as ABTS antioxidant capacity were also determined. In total, 52 phenolics compounds were identified with 9 compounds (derivatives of iriflophenone, apigenin C‐glycosides, luteolin O‐glycoside, isoflavones derivatives of iristectorigenin, dichotomitin, nigracin and irilone) never reported before in Iris spp. Differences in phenolic composition profile, pigments, soluble sugar, starch, total phenolics and flavonoids content and total antioxidant capacity were found among Iris species and different part of plants. Significant correlation between total phenolic content and antioxidant capacity was determined. The obtained results are comparable with those obtained for medical plants. These findings could be useful for fingerprinting characterization of Iris species and estimation of possible use in pharmaceutical industries. 相似文献
11.
M. Y. Ovando-Domínguez M. C. Luján-Hidalgo D. González-Mendoza A. A. Vargas-Díaz N. Ruiz-Lau F. A. Gutiérrez-Miceli C. A. Lecona-Guzmán 《Phyton》2019,88(2):139-147
Callus cultures of Annona muricata and Annona purpurea were induced in Murashige and Skoog (MS) medium supplemented with different concentrations of 1-naphthylacetic acid (NAA), 6-benzyladenine (BA) and 2,4-dichlorophenoxyacetic acid (2,4-D) utilized hypocotyls with explant. The highest percentage of callus formation was the treatment supplemented with 3 mg L-1 NAA for A. muricata (100%) while for A. purpurea in lower percentage (75%). BA stimulated the formation of shoots in all the evaluated concentrations, being the concentration of 2 mg L-1 the one that induced the greater formation of shoots for A. muricata (23 shoots/explant) and A. purpurea (28 shoots/explant). The content of total phenols, flavonoids and antioxidant activity was measured in the callus obtained from both species. The results showed that a higher content of total phenols was quantified in callus of A. purpurea (27.8 mg g-1 dw) compared to A. muricata (23.2 mg g-1 dw). The highest content of total flavonoids was observed in the callus of A. purpurea (8.0 μg g-1 dw). Antioxidant activity was determined by the 2,2-diphenyl-1-picrylhydracil radical assay. The concentration required for 50% inhibition (IC50) of the 2,2-diphenyl-1-picrylhydracil radicals were 4.22 μg mL-1 in methanolic extracts of callus of A. muricata, while in extracts of callus of A. purpurea was 2.86 μg mL-1, in both cases was greater than that found for leaves. Callus culture of the species studied in this work represents an alternative for the production of natural antioxidants. 相似文献
12.
13.
Alma R. López-Laredo Fanny D. Ramírez-Flores Gabriela Sepúlveda-Jiménez Gabriela Trejo-Tapia 《In vitro cellular & developmental biology. Plant》2009,45(5):550-558
Tecoma stans is a tropical plant from the Americas. Antioxidant activity and both phenolic compound and flavonoid total content were determined
for callus tissue of T. stans cultured in either a set photoperiod or in darkness. Callus lines from three explant types (hypocotyls, stem, and leaf) were
established on B5 culture medium supplemented with 0.5 μM 2,4-D and 5.0 μM kinetin. While leaf-derived callus grew slower
under a 16-h photoperiod (specific growth rate, μ = 0.179 d−1, t
D = 3.9 d) than in darkness (μ = 0.236 d−1, t
D = 2.9 d), it accumulated the highest amount (p < 0.05) of both phenolics (86.6 ± 0.01 mg gallic acid equivalents/g) and flavonoids (339.6 ± 0.06 mg catechin equivalents/g).
Similarly, antioxidant activity was significantly higher (p < 0.05) when callus was cultured in period light than when grown in extended darkness. Antioxidant activity measured with
a 2,20-azinobis (3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS)-based assay was 350.5 ± 15.8 mmol Trolox/g
extract for callus cultured under a defined photoperiod compared to 129.1 ± 7.5 mmol Trolox/g extract from callus cultured
in darkness. Content of phenolic compounds and flavonoids was in agreement with a better antioxidant power (EC50 = 450 μg extract/mg 1,1-diphenyl-2-picrylhydrazyl) and antiradical efficiency. Results of the present study show that calli
of T. stans are a source of compounds with antioxidant activity that is favored by culture under a set photoperiod. 相似文献
14.
The present study was conducted to determine the total hypericin contents of Hypericum triquetrifolium Turra. and Hypericum scabrum L. species
which are naturally distributed in the flora of Siirt province, Turkey. Hypericin
contents of Hypericum species grown in different geographical aspects (North,
South, East, and West), and it was measured at different harvest times (full blooming and post blooming period). In the current study, it has been determined that
total hypericin content varies considerably according to aspects, plant developmental stages (ontogenetic variance), and species. According to species x aspect
interaction, the highest total hypericin content was recorded from the west aspect
(3.13 mg/g) in Hypericum triquetrifolium, while, the lowest hypericin content was
also obtained from the west aspect (1.22 mg/g) in Hypericum scabrum. When the
highest total hypericin content was analyzed according to aspect x species x harvest time interaction, the highest total hypericin content was produced from
Hypericum triquetrifolium at the harvest of west aspect with 5.28 mg/g, while
the minimum amount of hypericin was obtained from the same aspect in Hypericum scabrum with 0.50 mg/g. In species x harvest time interaction, the highest
total hypericin content was obtained from the full bloom (3.10 mg/g) harvest in
Hypericum triquetrifolium, while the lowest hypericin was obtained from the full
bloom (1.26 mg/g) harvest in Hypericum scabrum. The data suggest that the average total hypericin content was 2.26 mg/g in Hypericum triquetrifolium and
1.28 mg/g in Hypericum scabrum. 相似文献
15.
Simiao Wang Xue Li Boxue Chen Shitong Li Jiali Wang Jing Wang Mingshuo Yang Xiaoyan Xu Hongda Wang Wenzhi Yang 《Phyton》2022,91(3):541-567
The complex composition of herbal metabolites necessitates the development of powerful analytical techniques aimed to identify the bioactive components. The seeds of Descurainia sophia (SDS) are utilized in China as a cough and asthma relieving agent. Herein, a dimension-enhanced integral approach, by combining ultra-high performance liquid chromatography/ion mobility-quadrupole time-of-flight mass spectrometry (UHPLC/IM-QTOF-MS) and intelligent peak annotation, was developed to rapidly characterize the multicomponents from SDS. Good chromatographic separation was achieved within 38 min on a UPLC CSH C18 (2.1 × 100 mm, 1.7 μm) column which was eluted by 0.1% formic acid in water (water phase) and acetonitrile (organic phase). Collision-induced dissociation-MS2 data were acquired by the data-independent high-definition MSE (HDMSE) in both the negative and positive electrospray ionization modes. A major components knockout strategy was applied to improve the characterization of those minor ingredients by enhancing the injection volume. Moreover, a self-built chemistry library was established, which could be matched by the UNIFI software enabling automatic peak annotation of the obtained HDMSE data. As a result of applying the intelligent peak annotation workflows and further confirmation process, a total of 53 compounds were identified or tentatively characterized from the SDS, including 29 flavonoids, one uridine derivative, four glucosides, one lignin, one phenolic compound, and 17 others. Notably, four-dimensional information related to the structure (e.g., retention time, collision cross section, MS1 and MS2 data) was obtained for each component by the developed integral approach, and the results would greatly benefit the quality control of SDS. 相似文献
16.
采用分光光度法测定了额济纳绿洲胡杨(Populus euphratica)的披针形叶、卵圆形叶、嫩枝、枝(D《5 mm)、枝(5~10 mm)、主干树皮、根(D《2 mm)、根(2~5 mm)和根(5~10 mm)9类器官中的总酚、黄酮和缩合单宁含量.结果表明,总酚含量较高的器官为皮(27.93 mg/s),叶、根、枝中总酚含量分别为17.64 mg/g(两类叶均值)、16.72mg/g(三类根均值)、12.19 mg/g(三类枝均值);黄酮含量较高的器官为皮(51.30 mg/g),叶、根、枝中黄酮含量分别为28.45 mg/g(两类叶均值)、39.99 mg/g(三类根均值)、23.67 mg/g(三类枝均值);根中缩合单宁含量较高,三类根均值为22.10 mg/g,皮、叶、枝中缩合单宁含量分别为8.41 mg/g、4.03 mg/g(两类叶均值)、4.47 mg/g(三类枝均值).披针形叶和卵圆形叶中酚类物质含量没有显著性差异(P》0.05);随着枝不断成熟,嫩枝、枝(D《5mm)、枝(5~10mm)中酚类物质逐渐减少;随着根直径减少,根中缩合单宁逐渐增加,细根(D《2mm)中的缩合单宁含量最高(25.95 mg/g).分析胡杨各器官中酚类物质含量与土壤水分的关系,结果表明卵圆形叶中酚类物质含量与土壤水分含量成显著负相关关系(P《0.05,总酚:r=-0.949;黄酮:r=-0.923;缩合单宁:r=-0.944).研究揭示了极端干旱地区胡杨各器官中酚类物质的变化规律,及其与环境因子的相互作用关系. 相似文献
17.
Hicham Mohti Francesco Cacciola Paola Dugo Luigi Mondello Abdelhamid Zaid 《Plant biosystems》2020,154(5):692-699
AbstractThis study was undertaken to investigate the effect of various solvents and techniques on the extractability of antioxidant compounds, particularly phenolics, from leaves and roots of Silene vulgaris subsp. macrocarpa grown wild in Morocco. Maceration and hot extraction with methanol or water and Soxhlet ethanol extraction were utilized. Aimed at establishing the potential safety of the extracts, Artemia salina lethality bioassay was performed. All the extracts were found to be non-toxic, except for the leaf Soxhlet ethanol. The antioxidant potential of the extracts was evaluated in vitro by DPPH, reducing power, and ferrous ions chelating activity assays. The leaf extracts displayed noticeable radical scavenging and chelating activities, and maceration with methanol (Mac-MeOH) resulted the most suitable extraction method for an effective recovery of antioxidants; further, the root Mac-MeOH extract demonstrated good chelating properties (IC50 = 335.49?±?0.70?µg/mL). Thus, leaf and root Mac-MeOH extracts were subjected to phytochemical investigations. The total phenolic, flavonoid and condensed tannin content was determined spectrophotometrically. Thirteen polyphenolic compounds were positively identified, by HPLC-PDA-ESI-MS, in the leaf extract for the first time, with p-coumaric acid derivatives being the most abundant ones (81%), whereas only catechin and procyanidin B1 were found in the root extract. 相似文献
18.
Ye Zhang Meng Lan Jin‐Peng Lü Jing‐Feng Li Kai‐Yue Zhang Hui Zhi Hui Zhang Jia‐Ming Sun 《化学与生物多样性》2020,17(1)
Chroogomphus rutilus is a rare fungal species that grows under pine trees and is now widely used as a functional food and pharmaceutical product. However, the chemical constituents and biological activities of Chroogomphus rutilus have been relatively limited. The present study aimed at determining the total polyphenols and flavonoids contents, biological activities and main phenolic compounds of Chroogomphus rutilus from different geographical origins at the stipe and pileus. The results suggested that Chroogomphus rutilus polyphenol extracts revealed a higher antioxidant, anti‐inflammatory, and cytotoxic activities, and there were significant differences between samples from different locations and regions. Correlation analysis showed that the contents of total polyphenols and flavonoids were significantly correlated with antioxidant and anti‐inflammatory activities. However, only the content of total flavonoids was significantly correlated with cytotoxicity, which means that the cytotoxicity of Chroogomphus rutilus polyphenol extracts may be regulated by flavonoids or other compounds. HPLC‐DAD analysis revealed that the main phenolic compound was protocatechuic acid, followed by baicalin, p‐hydroxyphenylacetic acid and p‐hydroxybenzoic acid, but comparing with the pileus extracts, the stipe extracts can be considered as a higher concentration of phenolic compounds. Therefore, antioxidant, anti‐inflammatory and cytotoxic activities of Chroogomphus rutilus polyphenol extracts could be due to the identified compounds. This study investigated a deep knowledge about the constituents and activities of Chroogomphus rutilus and provided the reference for its application in food and pharmaceutical. 相似文献
19.
Minghui Niu Heng Zhang Xiangyang Li Zhibao Hu Hongjiao Zhang Zhiru Xu Chunpu Qu Guanjun Liu 《Phyton》2023,92(7):2001-2015
KANADI (KAN) is a plant-specific gene that controlled the polarity development of lateral organs. It mainly acted on the abaxial characteristics of plants to make the lateral organs asymmetrical. However, it had been less identified in woody plants. In this study, the members of the KAN gene family in Populus trichocarpa were identified and analyzed using the bioinformatics method. The results showed that a total of 8 KAN family members were screened out, and each member contained the unique GARP domain and conserved region of the family proteins. Phylogenetic analysis and their gene structures revealed that all KAN genes from P. trichocarpa, Arabidopsis thaliana, and Nicotiana benthamiana could be divided into four subgroups, while the eight genes in P. trichocarpa were classified into three subgroups, respectively. The analysis of tissue-specific expression indicated that PtKAN1 was highly expressed in young leaves, PtKAN6 was highly expressed in young leaves and mature leaves, PtKAN2, PtKAN5, and PtKAN7 were highly expressed in nodes and internodes, PtKAN8 was highly expressed in roots, and PtKAN3 and PtKAN4 showed low expression levels in all tissues. Among them, PtKAN2 and PtKAN6, and PtKAN4 and PtKAN5 might have functional redundancy. Under high nitrogen concentrations, PtKAN2 and PtKAN8 were highly expressed in mature stems and leaves, respectively, while PtKAN4, PtKAN5, and PtKAN7 were highly expressed in roots. This study laid a theoretical foundation for further study of the KAN gene-mediated nitrogen effect on root development. 相似文献
20.
This study was carried out to investigate the phytochemical profiles of plant leaves, namely fresh and senescent leaves from Ficus deltoidea upon storage at room temperature. The phytochemical profile is of great importance, since this herb is widely used as traditional herbal medicine. Both chromatographic and mass spectrometric profiles of the plant extracts were fingerprinted using a high sensitivity hyphenated system consisting of liquid chromatography integrated with tandem mass spectrometer. Identical extraction protocol was used to extract phytochemicals from both leaf samples using 50% v/v methanolic aqueous solvent system. The plant extracts were determined for their total phenolic and flavonoid contents, as well as their antioxidant capacities based on radical scavenging, ferrous chelation, and ferric reducing power assays. The results showed that senescent leaf extracts exhibited higher antioxidant capacity than fresh leaf samples. This observation could be due to the higher number of phenolic compounds in the senescent leaf samples. The senescence of postharvest leaves was likely to consume organic acids including phenolic acids in the defense mechanism against the drought stress. However, flavonoids, particularly flavones and isoflavones, were abundant in the senescent leaf extracts. The findings may explain the significant pharmacological properties reported by previous investigators. 相似文献