正青霉属的两个中国新记录种

分离自广西的两个中国新记录种：布氏正青霉（Ｅｕｐｅｎｉｃｉｌｌｉｕｍ ｂｒｅｆｅｌｄｉａｎｕｍ（Ｂ．Ｄｏｄｇｅ）Ｓｔｏｌｋ＆Ｓｃｏｔｔ）,赭瑰色正青霉（Ｅｕｐｅｎｉｃｉｌｌｉｕｍ ｏｃｈｒｏｓａｌｍｏｎｅｕｍ Ｓｃｏｔｔ＆Ｓｔｏｌｋ）。根据广西的分离物对此两个种作了描述并附线条图。     

青霉属的三个新记录种

本文报道分离自中国山东和甘肃的三个青霉新记录种: 糙梗青霉Penicillium scabrorum Frisvad, Samson & Stolk, 栎生青霉Penicillium glandicola (Oud.) Seifert & Samson和狐粪青霉Penicillium vulpinum (Cooke & Massee) Sefert & Samson。     

中国西北地区青霉及其有性型初报

本文报道分离自中国西北地区—新疆、青海、甘肃和陕西的青霉菌Penicillium及其有性型正青霉Eupenicillium和篮状菌Talaromyces共47种,包括三个中国新记录种: 皮壳正青霉Eupenicillium crustaceum,真灰绿正青霉Eupenicillium euglaucum和西奈正青霉Penicillium sinaicum,并对此三个种作了描述并附线条图和子囊孢子的扫描电镜照片。     

中国西北地区青霉及其有性型初报

本文报道分离自中国西北地区－新疆,青海,甘肃和陕西的青霉菌Penicilkium及其有性型正青霉Eupenicillium和篮状菌Talaromyces共47种,包括三个中国新记录种：皮壳正青霉Eupenicillium crustaceum真灰绿正青霉Eupenicillium euglaucum和西奈正青霉Penicillum sinaicum,并对此三个种作了描述并附线条图和子囊孢子的扫描电镜照片。     

分离于贵州的一个青霉属新种

新种贵州青霉（Ｐｅｎｉｃｉｌｌｉｕｍ ｇｕｉｚｈｏｕａｎｕｍ ｓｐ．ｎｏｖ．）分离于贵州省发霉的竹笋,它和鲜红青霉（Ｐ．ｃｈｅｒｍｅｓｉｎｕｍ Ｐ．Ｂｉｏｕｒｇｅ）类似,但它的分子分生孢子结构形成缓慢,分生孢梗茎的顶端不膨大成囊状,瓶梗披针形及偶有双轮生帚状枝等特征,易于与后者相区分。本种与扁球青霉（Ｐ．ｏｂｌａｔｕｍ Ｊ．Ｉ．Ｐｉｔｔ ＆ Ａ．Ｄ．Ｈｏｃｋｉｎｇ）也有一些共同之处,如质地绳状,     

蜻蜓拟青霉多型性的扫描电镜观察及RAPD分析

通过电镜扫描观察,清楚地显示了蜻蜓拟青霉（Ｐａｅｃｉｌｏｍｙｃｅｓ ｏｄｏｎａｔａｅ Ｌｉｕ,Ｌｉａｎｇ ＆ Ｌｉｕ）的两种类型产孢结构,即拟青霉型椭圆形分生孢子的链状排列和枝顶孢霉型柱状分生孢子头状排列,同时将椭圆形分生孢子和柱状分生孢子进行单孢分离,对各单孢株作了培养特征观察,形态学观察和ＤＮＡ随机片段扩增分析。结果表明,在形态学上两种孢子单孢子株之间的培养特征和显微特征均无明显差异,同时具有     

蜻蜒拟青霉多型性的扫描电镜观察及RAPD分析

通过电镜扫描观察,清楚地显示了靖蜒拟青霉（Ｐａｅｃｉｌｏｍｙｃｅｓ　ｏｄｏｎａｔａｅ　Ｌｉｕ,Ｌｉａｎｇ＆Ｌｉｕ）的两种类型产孢结构,即拟青霉型椭圆形分生抱子的链状排列和校顶孢霉型柱状分生抱子头状排列。同时将椭圆形分生孢子和柱状分生孢子进行单孢分离,对各单孢株作了培养特征观察,形态学观察和ＤＮＡ随机片段扩增分析。结果表明,在形态学上两种孢子单孢子株之间的培养特征和显微特征均无明显差异,同时具有相同的ＤＮＡ随机扩增片段电泳谱。     

分离自海藻的青霉属三个中国新记录种

报道了分离自青岛沿海潮间带海藻的青霉属3个中国新记录种:产红青霉Penicillium rubens、苏门答腊青霉P.sumatrense和葡萄生青霉P.viticola,并对其进行了形态学描述和系统发育分析。研究菌株保存于中国海洋大学海洋生物标本室(OUCMB)。     

分离于贵州的一个青霉属新种

新种贵州青霉（Ｐｅｎｉｃｉｌｌｉｕｍ　ｇｕｉｚｈｏｕａｎｕｍ　ｓｐ．ｎｏｖ．）分离于贵州省发霉的竹笋,它和鲜红青霉（Ｐ．ｃｈｅｒｍｅｓｉｎｕｍＰ．Ｂｉｏｕｒｇｅ）类似,但它的分生孢子结构形成缓慢,分生抱梗茎的顶端不膨大成囊状,瓶梗披针形及偶有双轮生帚状枝等特征,易于与后者相区分．本种与扁球青霉（Ｐ．ｏｂｌａｔｕｍＪ．Ｉ．Ｐｉｔｔ　＆Ａ．Ｄ．Ｈｏｃｋｉｎｇ）也有一些共同之处,如质地绳状,帚状枝单轮生或偶有双轮生,瓶梗披针形等。然而,其分生孢．梗茎的壁平滑,分生孢子呈长椭圆形,这些特征是扁球青霉所不具备的．     

拟青霉属一新种及其杀蚜活性

从病死棉蚜（AphisgossypiiGlover)分离到拟青霉属一新种一灰绿拟青霉（PaecilomycesgriseiviridisM．X．Dai）。在查氏琼脂和PDA上菌落呈灰绿色。瓶梗1．8～3．2×5．6～10．3μm,基部椭圆形膨大或呈圆柱状,上部明显变细;分生孢子纺锤形,单胞,壁光滑,1．2～1．8×2．8～4．4μm。分生孢子对蚜虫具有侵染力;该菌发酵液（离心后）亦具有一定的杀蚜活性。     

Partial purification and characterization of a 1,3-β-d-glucanase fromGanoderma tsugae

Fermentation conditions are described for the production of the antitumor antibiotic 7-(S)-brefeldin-A (brefeldin-A) in liquid culture byEupenicillium brefeldianum, (B. Dodge) Stolk and Scott, ATCC 58665. An analytical hplc method was developed which allowed rapid quantitation of the compound during fermentation. A kilogram of brefeldin-A was isolated from a fermentation at the 6800-liter scale.     

Citreoviridin levels in Eupenicillium ochrosalmoneum-infested maize kernels at harvest

Citreoviridin contents were measured in eight bulk samples of maize kernels collected from eight fields immediately following harvest in southern Georgia. Citreoviridin contamination in six of the bulk samples ranged from 19 to 2,790 micrograms/kg. In hand-picked samples the toxin was concentrated in a few kernels (pick-outs), the contents of which were stained a bright lemon yellow (range, 53,800 to 759,900 micrograms/kg). The citreoviridin-producing fungus Eupenicillium ochrosalmoneum Scott & Stolk was isolated from each of these pick-out kernels. Citreoviridin was not detected in bulk samples from two of the fields. Aflatoxins were also present in all of the bulk samples (total aflatoxin B1 and B2; range, 7 to 360 micrograms/kg), including those not containing citreoviridin. In Biotron-grown maize ears that were inoculated with E. ochrosalmoneum through a wound made with a toothpick, citreoviridin was concentrated primarily in the wounded and fungus-rotted kernels (range, 142,000 to 2,780,000 micrograms/kg). Samples of uninjured kernels immediately adjacent to the wounded kernel (first circle) had less than 4,000 micrograms of citreoviridin per kg, while the mean concentration of toxin in kernel samples representing the next row removed (second circle) and all remaining kernels from the ear was less than 45 micrograms/kg. Animal toxicosis has not been linked to citreoviridin-contaminated maize.     

Citreoviridin levels in Eupenicillium ochrosalmoneum-infested maize kernels at harvest.

Citreoviridin contents were measured in eight bulk samples of maize kernels collected from eight fields immediately following harvest in southern Georgia. Citreoviridin contamination in six of the bulk samples ranged from 19 to 2,790 micrograms/kg. In hand-picked samples the toxin was concentrated in a few kernels (pick-outs), the contents of which were stained a bright lemon yellow (range, 53,800 to 759,900 micrograms/kg). The citreoviridin-producing fungus Eupenicillium ochrosalmoneum Scott & Stolk was isolated from each of these pick-out kernels. Citreoviridin was not detected in bulk samples from two of the fields. Aflatoxins were also present in all of the bulk samples (total aflatoxin B1 and B2; range, 7 to 360 micrograms/kg), including those not containing citreoviridin. In Biotron-grown maize ears that were inoculated with E. ochrosalmoneum through a wound made with a toothpick, citreoviridin was concentrated primarily in the wounded and fungus-rotted kernels (range, 142,000 to 2,780,000 micrograms/kg). Samples of uninjured kernels immediately adjacent to the wounded kernel (first circle) had less than 4,000 micrograms of citreoviridin per kg, while the mean concentration of toxin in kernel samples representing the next row removed (second circle) and all remaining kernels from the ear was less than 45 micrograms/kg. Animal toxicosis has not been linked to citreoviridin-contaminated maize.     

The structure and biogenesis of desoxyverrucarin E,a metabolite of Eupenicillium hirayamae

Desoxyverrucarin E is the main nitrogenous metabolite of Eupenicillium hirayamae. On the basis of chemical and spectroscopic data the compound was identified as 3-acetyl-4-methylpyrrole. Radiochemical data showed that the compound is biogenetically derived from acetate.     

四个嗜热真菌中国新记录种

报道四个嗜热真菌中国新记录种:嗜热子囊菌光孢变种Thermoascus aurantiacus var.levisporus;埃默森篮状菌Talaromyces emersonii;杜邦青霉Penicillium dupontii及丝衣霉状拟青霉Paecilomyces byssochlamydioides,对其进行了描述和讨论。研究标本保存在山东农业大学植物病理学标本室(HSAUP)。     

嗜热子囊菌两个中国新记录种

对采自云南的标本进行研究,描述了两种嗜热子囊菌中国新记录种,分别是太瑞斯梭孢壳霉[Thielavia terrestris (Apinis) Malloch & Cain]和丝衣霉状篮状菌[Talaromyces byssochlamydioides Stolk & Samson],附插图。研究标本保存在山东农业大学植物病理学标本室(HSAUP)。     

草莓炭疽病生防菌株MT-06的鉴定及生物学特性

对草莓炭疽病具有良好生防效果的菌株MT-06的抑菌谱、生物学特性及其分类地位进行了研究。结果表明:MT-06具有广谱抗菌性,对多种病原菌具有拮抗作用;其营养生长的最适条件是:在PDA或PSA培养基上,28-35℃,初始pH为5,24h全光照;在查氏培养基上,25-28℃,24h全光照条件下孢子产量最高。经形态观察和rDNA-ITS序列及β-tubulin序列分析,将该菌株鉴定为Talaromyces flavus(Klcker)Stolk Samson。