共查询到10条相似文献,搜索用时 163 毫秒
1.
2.
3.
Li Xu Yijun Yuan Lin Zhang Li Wan Yusheng Zheng Peng Zhou Dongdong Li 《Tree Genetics & Genomes》2011,7(5):999-1010
Suppression subtracted hybridization (SSH) and dot blotting were used to identify differential gene expression in the mesocarp
and kernel of oil palm nuts. The different types of nut tissue show differences in fatty acid anabolism and the synthesis
of other important compounds. In total, 302 clones from forward SSH libraries and 238 clones from reverse SSH libraries were
identified following differential screening, respectively. Among these, 120 clones from the forward SSH library and 81 clones
from the reverse SSH library, showed tenfold or more differential expression levels, and were sequenced. Sequence analysis
revealed that 76 clones (28 from the forward SSH library and 48 from the reverse SSH library) represent non-redundant cDNA
inserts. The differential expression of 39 subset genes in the two different tissues was further confirmed by RT-PCR analysis.
Functionally annotated blasting against the GenBank non-redundant protein database classified all 76 candidate genes into
six categories, according to their putative functions. Interestingly, our results show that a group of significantly differentially
expressed genes are involved in processes associated with oil palm nut maturation, such as the synthesis of medium-chain saturated
fatty acids and phytic acid, nut development, and stress/defense responses. This study describes some relationships between
gene expression and metabolic pathways in mature oil palm nuts, and contributes to our understanding of oil palm nut ESTs. 相似文献
4.
5.
6.
7.
8.
9.
Millar AA Jacobsen JV Ross JJ Helliwell CA Poole AT Scofield G Reid JB Gubler F 《The Plant journal : for cell and molecular biology》2006,45(6):942-954
We have investigated the relationship between seed dormancy and abscisic acid (ABA) metabolism in the monocot barley and the dicot Arabidopsis. Whether dormant (D) or non-dormant (ND), dry seed of Arabidopsis and embryos of dry barley grains all had similarly high levels of ABA. ABA levels decreased rapidly upon imbibition, although they fell further in ND than in D. Gene expression profiles were determined in Arabidopsis for key ABA biosynthetic [the 9-cis epoxycarotenoid dioxygenasegene family] and ABA catabolic [the ABA 8'-hydroxylase gene family (CYP707A)] genes. Of these, only the AtCYP707A2 gene was differentially expressed between D and ND seeds, being expressed to a much higher level in ND seeds. Similarly, a barley CYP707 homologue, (HvABA8'OH-1) was expressed to a much higher level in embryos from ND grains than from D grains. Consistent with this, in situ hybridization studies showed HvABA8'OH-1 mRNA expression was stronger in embryos from ND grains. Surprisingly, the signal was confined in the coleorhiza, suggesting that this tissue plays a key role in dormancy release. Constitutive expression of a CYP707A gene in transgenic Arabidopsis resulted in decreased ABA content in mature dry seeds and a much shorter after-ripening period to overcome dormancy. Conversely, mutating the CYP707A2 gene resulted in seeds that required longer after-ripening to break dormancy. Our results point to a pivotal role for the ABA 8'-hydroxylase gene in controlling dormancy and that the action of this enzyme may be confined to a particular organ as in the coleorhiza of cereals. 相似文献