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1.
金针菇FV908菌株液体培养工艺   总被引:1,自引:1,他引:0  
沈秀荣 《生物技术》2001,11(6):23-25
通过单因子试验统计分析,优化筛选了适于金针菇(Flammulina velatipes)FV908的适宜培养基和摇瓶培养条件,结果表明,其适宜的液体培养基组成为5.0%玉米粉,2.0%麸皮,0.1%KHOP4,0.05%MgSO4*7H2O,10μgVB1/100ml,50μgVB2/100ml;适宜的摇瓶培养条件为培养基的起始pH6.0~7.0,500ml摇瓶装量为150ml,接种量为10%,培养温度25℃,摇床转速为120r/min,菌丝干收率4.1g/100ml.  相似文献   

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松口蘑深层发酵工艺的研究   总被引:16,自引:0,他引:16  
对松口蘑深层发酵工艺进行系统研究。通过正交试验初步确定松口蘑适宜的培养基组成 :玉米粉 30g,葡萄糖 1 0g ,豆饼粉 1 0g ,玉米浆 1 0g ,KH2 PO41g ,定容至 1L。适宜发酵条件 :最适生长温度为 2 5℃ ,摇瓶转速为 1 60r/min ,最适pH为 5 0 ,最适接种量为 1 0 % ,装液量 1 2 0mL/ 5 0 0mL摇瓶培养 1 0d ,菌体生物量达 1 2 94g/L。  相似文献   

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微量元素铬载体酵母摇瓶发酵研究   总被引:5,自引:0,他引:5  
实验选择了啤酒酵母 2 0 16菌株为铬载体酵母生产菌株 ,通过摇瓶发酵实验 ,对三氯化铬的添加工艺进行了研究探索。确定了铬酵母发酵培养的适宜加铬条件 :在发酵开始的8h内分批流加三氯化铬 ,总加入量为 5× 104mol/L ,经过 12h摇瓶发酵可得铬酵母的生物量为 1 5 5 g/ 10 0mL ,酵母含铬量为 1,10 0 μg/ g以上 ,酵母细胞对铬离子利用效率是 6 0 %以上。  相似文献   

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对茶树菇(Agrocybe cylindracea)液体摇瓶培养的碳氮源、接种量、装液量以及培养时间进行了研究,筛选出了茶树菇液体培养的适宜条件。尝试将外源植物激素应用于茶树菇液体培养中,探讨其加快菌丝体繁殖的可能性。结果表明,茶树菇液体发酵适宜的培养基为:马铃薯20%+小麦粉2%+酵母粉0.1%+MgSO_4 0.3%+KH_2PO_40.2%+VB_1 10 mg/L。培养条件以250 mL摇瓶装液量120 mL,接种量10 mL/100 mL,培养9 d终止发酵为宜。培养基中添加10 mg/L的外源激素NAA,能显著提高菌丝的生物量。  相似文献   

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杏鲍菇液体培养研究初报   总被引:4,自引:1,他引:3  
采用液体摇瓶培养方法,对杏鲍菇适宜的液体培养基和液全培养条件(适宜温度,摇瓶装量,摇瓶转速,接种量,培养基初始pH等)进行了探讨。结果表明,杏鲍 菇适宜的液体培养基为:葡萄糖3%,蛋白胨0.2%,KH2PO40.05%,MgSO40.05%,pH6.5,适宜的液全培养条件为:培养温度25℃,培养基的初始pH6.5,摇瓶装量100-140ml/500ml,摇瓶转速150r/min,接种量10%,最佳培养时间7d。  相似文献   

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L-缬氨酸发酵条件的研究   总被引:13,自引:1,他引:12  
报道了L 缬氨酸高产菌XQ 6(Leu1AHVrα ABhr2 TAhr)摇瓶发酵条件的研究结果。试验结果表明 ,当发酵培养基中葡萄糖、(NH4 ) 2 SO4 、KH2 PO4 、MgSO4 ·H2 O、玉米浆和生物素的最适用量分别为 1 4 %、5 %、0 .1 %、0 .0 5 %、0 .5 %和2 5 μg/L时 ,经发酵培养 72h ,L 缬氨酸积累可达 5 8g/L ,最高为 62g/L。  相似文献   

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蛹虫草液体培养条件的优化及生长动力学考察   总被引:9,自引:0,他引:9  
为了优化蛹虫草菌的液体培养条件 ,对液体培养菌丝的生长情况进行了研究。采用摇瓶培养的方法 ,以发酵得率为指标确定培养条件。结果表明 :优化培养基成分确定为蛋白胨 1.5 % ,葡萄糖 2 % ,KH2 PO4 0 .15 % ,Mg SO40 .0 5 %、VB1 5 m g/ L .2 ,4 - D 2 mg/ L .该培养基下发酵得率达 1.4~ 2 .2 g/ 10 0 m L ;考察了蛹虫草菌液体培养的外部条件 ,确定为 :装料系数为 30 % ,培养基 p H为 5 .5 ,接种量为 0 .0 3g,摇床振荡频率为 12 0 r· m in- 1 ;利用优化培养基得到了生长代谢曲线。在相同条件下 ,优化培养基比原来培养基的发酵得率提高了 4 .5 % ,研究初步得到蛹虫草菌液体培养条件和生长动力学 ,为工业化生产提供了一定的依据  相似文献   

8.
β-葡聚糖酶高产菌株BS9418F的选育及其发酵条件的研究   总被引:13,自引:0,他引:13  
经60 Coγ射线辐照处理获得的诱变菌株芽孢杆菌BS9418F ,其产酶活力比出发菌株提高 30 %以上。该菌株以大麦粉 7%、玉米粉 3%、豆粕 3%及适量无机盐为培养基最佳配比 ,其最适培养条件为 :培养基初始 pH 7.0 ,摇瓶装量 5 0mL/ 30 0mL三角瓶 ,种龄 16~ 2 0h ,接种量 2 %~ 3% ,培养温度 36~ 37℃ ,发酵周期 40h。在优化条件下 ,摇瓶发酵产 β 葡聚糖酶活力高达 5 5 0 0u/mL以上 ,比出发菌株初始发酵水平提高了 4倍以上  相似文献   

9.
灰树花深层发酵工艺条件的研究   总被引:13,自引:0,他引:13  
灰树花摇瓶发酵较佳条件 :QF培养基 ,2 5℃ ,pH4 5,装量 60mL/50 0mL三角瓶 ,转速 1 0 0r/min。在种子培养基中加 0 4%的CMC ,可增加种子液菌丝的生长点 ,从而提高菌丝量。在 1 0L气升式发酵罐上放大试验 ,菌丝量对初糖的生物转化率在 2 4 %以上 ,对耗糖的转化率达 435%。菌丝体中多糖含量达 1 0 2 % ,发酵液中多糖含量为 1 38%。  相似文献   

10.
从数株红酵母中选出 1株产类胡萝卜素能力较强的红酵母RY 98(生物量、类胡萝卜素含量和产量分别为19.9g/L ,334 .8μg/ g和 6 .7mg/L) ;研究了该菌株产类胡萝卜素的最适营养与环境条件 ,获得了最佳的发酵生理学条件 :葡萄糖 40 g/L ,(NH4 ) 2 SO4 10 g/L ,酵母膏 3g/L ,蕃茄汁 2mL/L ,花生油 0 .5mL/L ,接种量 30mL/L ,初始pH 6 .0和通气量 (培养基装量 ) 4 0mL/ 2 5 0mL。在此初步优化的培养条件下 ,红酵母RY 98经 72h摇瓶发酵其生物量、类胡萝卜素含量和产量分别可达 2 6 .8g/L ,386 .9μg/ g和 10 .4mg/L ,依次比初筛中提高了 34 .7% ,15 .6 %和 5 5 .2 %。  相似文献   

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Comprises species occurring mostly in subtidal habitats in tropical, subtropical and warm-temperate areas of the world. An analysis of the type species, V. spiralis (Sonder) Lamouroux ex J. Agardh, a species from Australia, establishes basic characters for distinguishing species in the genus. These characters are (1) branching patterns of thalli, (2) flat blades that may be spiralled on their axis, (3) width of the blade, (4) primary or secondary derivation of sterile and fertile branchlets and (5) position of sterile and fertile branchlets on the thalli. Application of the latter two characters provides an important basic method for separation of species into three major groups. Osmundaria , a genus known only in southern Australia, was studied in relation to Vidalia , and its separation from the Vidalia assemblage is not accepted. Species of Vidalia therefore are transferred to the older genus name, Osmundaria. Two new species, Osmundaria papenfussii and Osmundaria oliveae are described from Natal. Confusion in the usage of the epithet, Vidalia fimbriala Brown ex Turner has been clarified, and Vidalia gregaria Falkenberg, described as an epiphyte on Osmundaria pro/ifera Lamouroux, is revealed to be young branches of the host, Osmundaria prolifera.  相似文献   

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Fifteen chromosome counts of six Artemisia taxa and one species of each of the genera Brachanthemum, Hippolytia, Kaschgaria, Lepidolopsis and Turaniphytum are reported from Kazakhstan. Three of them are new reports, two are not consistent with previous counts and the remainder are confirmations of very scarce (one to four) earlier records. All the populations studied have the same basic chromosome number, x = 9, with ploidy levels ranging from 2x to 6x. Some correlations between ploidy level, morphological characters and distribution are noted.  相似文献   

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Curcumin is the yellow pigment of turmeric that interacts irreversibly forming an adduct with thioredoxin reductase (TrxR), an enzyme responsible for redox control of cell and defence against oxidative stress. Docking at both the active sites of TrxR was performed to compare the potency of three naturally occurring curcuminoids, namely curcumin, demethoxy curcumin and bis-demethoxy curcumin. Results show that active sites of TrxR occur at the junction of E and F chains. Volume and area of both cavities is predicted. It has been concluded by distance mapping of the most active conformations that Se atom of catalytic residue SeCYS498, is at a distance of 3.56 from C13 of demethoxy curcumin at the E chain active site, whereas C13 carbon atom forms adduct with Se atom of SeCys 498. We report that at least one methoxy group in curcuminoids is necessary for interation with catalytic residues of thioredoxin. Pharmacophore of both active sites of the TrxR receptor for curcumin and demethoxy curcumin molecules has been drawn and proposed for design and synthesis of most probable potent antiproliferative synthetic drugs.  相似文献   

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正Dear Editor,In December 2019, a novel human coronavirus caused an epidemic of severe pneumonia(Coronavirus Disease 2019,COVID-19) in Wuhan, Hubei, China(Wu et al. 2020; Zhu et al. 2020). So far, this virus has spread to all areas of China and even to other countries. The epidemic has caused 67,102 confirmed infections with 1526 fatal cases  相似文献   

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