中国粘菌XII:无丝菌属一新种

本文描述了无丝菌属一新种:网孢无丝菌Licea reticulospora H.-z. Li et Y. Li sp. nov.此种孢子具网纹,与本属中巳知的种均不相同,故定为新种。模式标本保存在中国科学院微生物研究所真菌标本室。     

葡萄属一新名和一新种

描述了自甘肃南部发现的葡萄属一新种,文县蘡薁(Vitis wenxianensis W. T. Wang)。此新种与特产浙江的三出蘡薁[V. bryoniifolia Bunge var. ternata(W. T. Wang)C. L. Li]有很近的亲缘关系,二者可能是由蘡薁(V. bryoniifolia Bunge)衍生而出的一对姊妹群。基于上述认识,将三出蘡薁由变种提升至种的等级,但由于存在一个于1871年发表的种名(V. ternata Baker),因此,必须为三出蘡薁拟定一新名。     

广西大明山地区台蚱属一新种记述（直翅目：蚱总科）

记述采自广西大明山地区蚱科Tetrigidae台蚱属Formosatettix 1新种,即大明山台蚱Formosatettix damingshanensis Li, Deng & Zheng sp. nov.。本新种近似于大瑶山台蚱Formosatettix dayaoshanensis Zheng et Jiang, 1998,主要区别在于：新种体小,♂10–10.5 mm, ♀10.5–11 mm;头顶明显突出于复眼前缘之前,前缘弧形;颜面隆起纵沟在触角之间的宽度与触角基节等宽;前足股节下缘具2齿突,波状;后足跗节第1节长度为第2、3节之和的1.5倍。模式标本保存在陕西师范大学动物研究所标本室。     

中国钩瓣叶蜂属一新种及M. coxalis种团中国种类检索表（膜翅目：叶蜂科）

记述中国钩瓣叶蜂属Macrophya Dahlbom 碟片钩瓣叶蜂种团Macrophya coxalis group 1新种：侧斑钩瓣叶蜂Macrophya latimaculana Li, Dai & Wei sp. nov.;简要讨论了M. coxalis group的主要鉴别特征,编制了M. coxalis group中国已知种类分种检索表。该新种与中国分布的寡斑钩瓣叶蜂M. oligomaculella Wei & Zhu, 2009最近似,但新种唇基与上唇均完全白色;腹部第1背板端部1/3白色,第2~8背板两侧具显著宽横白斑;各足基节大部白色,前中足基节基缘和后足基节腹侧仅基部外侧三角形大斑黑色;后足股节大部黑色,腹侧具明显白带等特征,与后者不同。     

中国粘菌Ⅶ:团毛菌科的新种和新记录种

本文报告了团毛菌科中的二个新种:Perichaena membranea sp. nov.; Perichaena poronema sp. nov及二个国内新纪录种:Trichia erecta Rex和Arcyria abietina (A.Wigand) N. E. Nannenga-Bremekamp.新种有拉T文描述及扫描电镜照片,讨论了新种与相近种的关系。模式标本收藏于HMAS北京和HJAU长春。     

中国粘菌X:中国团毛菌科补记

本文补报了中国团毛菌科四个种,弱小团网菌Arcyria exigua sp. nov.和聚生团网菌Arcyria aggregata sp. nov.为新种,瑞士团网菌Arcyria helvetica和纹丝半网菌Hemitrichia intorta为中国新记录种。文中对二新种进行了描述,附有形态特征电镜扫描照片,并讨论了与相似种间的区别。新种的模式标本保存在中国科学院微生物研究所真菌标本室。     

中国粘菌Ⅵ:散丝菌属一新种

本文报告了粘菌一新种,小囊散丝菌Dianema microsporangium sp. nov.,讨论了新种与本其它种的区别,模式标本保存在中国科学院微生物研究所真菌标本室.散丝菌属在中国首次报导。     

四川重楼属植物2新记录变种

报道了四川重楼属植物2新分布变种,分别是宽瓣球药隔重楼(Paris fargesii var. latipetala H. Li et V. G. Soukup)和红果五指莲(Paris axialis H. Li var. rubra H. H. Zhou, K. Y. Wu et R. Tao)。其中红果五指莲为贵州特有类群,且为孤点分布。四川新分布的发现对于重楼属植物的系统分类和迁徙演化研究具有重要意义。     

獐耳细辛属重瓣川鄂獐耳细辛在陕西省的新分布

该文报道了獐耳细辛属植物重瓣川鄂獐耳细辛(Hepatica henryi f. pleniflora Xiao D. Li et J. Q. Li)的新分布区——陕西省岚皋县南宫山国家森林公园及镇坪县化龙山国家森林公园,将该种的分布区向西北扩展到了陕西省的巴山地区,丰富了陕西省新增植物分布记录,为巴山地区的物种多样性研究提供了证据。     

中国假尾孢属的研究V

本文报导中国假尾孢属的30个种,其中有2个新种:透骨草假尾孢(Pseudocercospora phrymae),槐假尾孢(P. sophorae), 6个新组合:无花果假尾孢(P. fici),爵床假尾孢(P. justiciae),木犀生假尾孢(P. osmanthicola),海桐花假尾孢(P. pittospori),色柱假尾孢(P. polygonorum),花椒假尾孢(P. xanthoxyli)和5个中国新记录。文中对新种和新组合进行了描述并绘图,其他种作了简要描述或讨论。研究的标本保存在中国科学院微生物研究所真菌标本室(HMAS)。     

Sero-systematics ofCytisus sect.Trianthocytisus (Fabaceae)

The serological reaction of seed proteins provides evidence for a partly new systematic arrangement ofCytisus sect.Trianthocytisus and ofCytisus s.l. Proposed modifications agree with recent advances in morphological taxonomy. Sect.Trianthocytisus includes only two species,C. villosus andC. aeolicus. Its position is central within the genus, and this fact agrees with the proposed retypification ofCytisus (type species:C. villosus).C. emeriflorus, formerly included in the same section, constitutes the monospecific sect.Emeroides, which is intermediate towards the genusLembotropis. This is serologically isolated and includes onlyL. nigricans. It is confirmed thatC. sessilifolius should be removed from the genusCytisus as a monospecific genus:Cytisophyllum Lang which is closely allied toHesperolaburnum and toPodocytisus, the most primitive genera ofGenisteae.     

Phylogenetic investigations of Sordariaceae based on multiple gene sequences and morphology

The family Sordariaceae incorporates a number of fungi that are excellent model organisms for various biological, biochemical, ecological, genetic and evolutionary studies. To determine the evolutionary relationships within this group and their respective phylogenetic placements, multiple-gene sequences (partial nuclear 28S ribosomal DNA, nuclear ITS ribosomal DNA and partial nuclear β-tubulin) were analysed using maximum parsimony and Bayesian analyses. Analyses of different gene datasets were performed individually and then combined to generate phylogenies. We report that Sordariaceae, with the exclusion Apodus and Diplogelasinospora, is a monophyletic group. Apodus and Diplogelasinospora are related to Lasiosphaeriaceae. Multiple gene analyses suggest that the spore sheath is not a phylogenetically significant character to segregate Asordaria from Sordaria. Smooth-spored Sordaria species (including so-called Asordaria species) constitute a natural group. Asordaria is therefore congeneric with Sordaria. Anixiella species nested among Gelasinospora species, providing further evidence that non-ostiolate ascomata have evolved from ostiolate ascomata on several independent occasions. This study agrees with previous studies that show heterothallic Neurospora species to be monophyletic, but that homothallic ones may have a multiple origins. Although Gelasinospora and Neurospora are closely related and not resolved as monophyletic groups, there is insufficient evidence to place currently accepted Gelasinospora and Neurospora species into the same genus.     

A molecular phylogeny of Hebeloma species from Europe

In order to widen the scope of existing phylogenies of the ectomycorrhizal agaric genus Hebeloma a total of 53 new rDNA ITS sequences from that genus was generated, augmented by sequences retrieved from GenBank, and analysed using Bayesian, strict consensus and neighbour joining methods. The lignicolous Hebelomina neerlandica, Gymnopilus penetrans, and two species of Galerina served as outgroup taxa. Anamika indica, as well as representatives of the genera Hymenogaster and Naucoria, were included to test the monophyly of Hebeloma, which is confirmed by the results. Hebeloma, Naucoria, Hymenogaster and Anamika indica cluster in a strongly supported monophyletic hebelomatoid clade. All trees largely reflect the current infrageneric classification within Hebeloma, and divide the genus into mostly well-supported monophyletic groups surrounding H. crustuliniforme, H. velutipes, H. sacchariolens, H. sinapizans, and H. radicosum, with H. sarcophyllum being shown at an independent position; however this is not well supported. The section Indusiata divides with strong support into three groups, the position of the pleurocystidiate Hebeloma cistophilum suggests the possible existence of a third subsection within sect. Indusiata. Subsection Sacchariolentia is raised to the rank of section.     

Phylogeny of cardinalfishes (Teleostei: Gobiiformes: Apogonidae) and the evolution of visceral bioluminescence

The cardinalfishes (Apogonidae) are a diverse clade of small, mostly reef-dwelling fishes, for which a variety of morphological data have not yielded a consistent phylogeny. We use DNA sequence to hypothesize phylogenetic relationships within Apogonidae and among apogonids and other acanthomorph families, to examine patterns of evolution including the distribution of a visceral bioluminescence system. In conformance with previous studies, Apogonidae is placed in a clade with Pempheridae, Kurtidae, Leiognathidae, and Gobioidei. The apogonid genus Pseudamia is recovered outside the remainder of the family, not as sister to the superficially similar genus Gymnapogon. Species sampled from the Caribbean and Western Atlantic (Phaeoptyx, Astrapogon, and some Apogon species) form a clade, as do the larger-bodied Glossamia and Cheilodipterus. Incidence of visceral bioluminescence is found scattered throughout the phylogeny, independently for each group in which it is present. Examination of the fine structure of the visceral bioluminescence system through histology shows that light organs exhibit a range of morphologies, with some composed of complex masses of tubules (Siphamia, Pempheris, Parapriacanthus) and others lacking tubules but containing chambers formed by folds of the visceral epithelium (Acropoma, Archamia, Jaydia, and Rhabdamia). Light organs in Siphamia, Acropoma, Pempheris and Parapriacanthus are distinct from but connected to the gut; those in Archamia, Jaydia, and Rhabdamia are simply portions of the intestinal tract, and are little differentiated from the surrounding tissues. The presence or absence of symbiotic luminescent bacteria does not correlate with light organ structure; the tubular light organs of Siphamia and chambered tubes of Acropoma house bacteria, those in Pempheridae and the other Apogonidae do not.     

Phylogeny and Megasystematics of Phagotrophic Heterokonts (Kingdom Chromista)

Heterokonts are evolutionarily important as the most nutritionally diverse eukaryote supergroup and the most species-rich branch of the eukaryotic kingdom Chromista. Ancestrally photosynthetic/phagotrophic algae (mixotrophs), they include several ecologically important purely heterotrophic lineages, all grossly understudied phylogenetically and of uncertain relationships. We sequenced 18S rRNA genes from 14 phagotrophic non-photosynthetic heterokonts and a probable Ochromonas, performed phylogenetic analysis of 210–430 Heterokonta, and revised higher classification of Heterokonta and its three phyla: the predominantly photosynthetic Ochrophyta; the non-photosynthetic Pseudofungi; and Bigyra (now comprising subphyla Opalozoa, Bicoecia, Sagenista). The deepest heterokont divergence is apparently between Bigyra, as revised here, and Ochrophyta/Pseudofungi. We found a third universal heterokont signature sequence, and deduce three independent losses of ciliary hairs, several of 1-2 cilia, 10 of photosynthesis, but perhaps only two plastid losses. In Ochrophyta, heterotrophic Oikomonas is sister to the photosynthetic Chrysamoeba, whilst the abundant freshwater predator Spumella is biphyletic; neither clade is specifically related to Paraphysomonas, indicating four losses of photosynthesis by chrysomonads. Sister to Chrysomonadea (Chrysophyceae) is Picophagea cl. nov. (Picophagus, Chlamydomyxa). The diatom-parasite Pirsonia belongs in Pseudofungi. Heliozoan-like actinophryids (e.g. Actinosphaerium) are Opalozoa, not related to pedinellids within Hypogyristea cl. nov. of Ochrophyta as once thought. The zooflagellate class Bicoecea (perhaps the ancestral phenotype of Bigyra) is unexpectedly diverse and a major focus of our study. We describe four new biciliate bicoecean genera and five new species: Nerada mexicana, Labromonas fenchelii (=Pseudobodo tremulans sensu Fenchel), Boroka karpovii (=P. tremulans sensu Karpov), Anoeca atlantica and Cafeteria mylnikovii; several cultures were previously misidentified as Pseudobodo tremulans. Nerada and the uniciliate Paramonas are related to Siluania and Adriamonas; this clade (Pseudodendromonadales emend.) is probably sister to Bicosoeca. Genetically diverse Caecitellus is probably related to Anoeca, Symbiomonas and Cafeteria (collectively Anoecales emend.). Boroka is sister to Pseudodendromonadales/Bicoecales/Anoecales. Placidiales are probably divergent bicoeceans (the GenBank Placidia sequence is a basidiomycete/heterokont chimaera). Two GenBank ‘opalinid’ sequences are fungal; Pseudopirsonia is cercozoan; two previous GenBank ‘Caecitellus’ sequences are Adriamonas. Electronic Supplementary Material Electronic Supplementary material is available for this article at and accessible for authorised users. [Reviewing Editior: Patnck J. Keeling]     

Non-specificity of a colorimetric method for the estimation of N-acetyl-d-glucosamine

The colorimetric method of Reissig et al. for the estimation of N-acetylamino sugars, is often used as a specific method for the quantification of the N-acetyl-d-glucosamine. Although this assay is more sensitive to the monomer, it recognizes all soluble N-acetyl-d-glucosamine oligomers. This result is very important because this method is extensively used in biology for the estimation of chitinolytic activity.     

Phylogenetic relationships of Auriculoscypha based on ultrastructural and molecular studies

The phylogeny of Auriculoscypha anacardiicola, an associate of scale insects in India, is investigated using subcellular characters and MP and Bayesian analyses of combined nuLSU-rDNA, nuSSU-rDNA and 5.8S rDNA sequence data. It has simple septa with a pulley-wheel-shaped pore plug, which is diagnostic of phytoparasitic members of the Pucciniomycetes, and hyphal wall break on branching, a phenomenon unique to some simple septate heterobasidiomycetes. The septal ultrastructure of A. anacardiicola is similar to that of the genus Septobasidium. The close relationship to Septobasidium is also confirmed by rDNA sequence analyses. The polyphyletic nature of the order Platygloeales, noted in earlier studies, is evident from the present molecular analysis as well. The placement of Auriculoscypha in the Platygloeales can no longer be justified and both ultrastructural and molecular evidence strongly support the placement of Auriculoscypha in the Septobasidiales.     

Phylogenetic relationships in theCampanulales based onrbcL sequences

Phylogenetic relationships within the angiosperm orderCampanulales were investigated by comparative sequencing of the chloroplast generbcL. CompleterbcL sequences were obtained for ten species in six families within the order. These data were analyzed along with previously publishedrbcL sequences from other taxa (for a total of 117 species) within the subclassAsteridae and outgroups, producing 32 equally parsimonious trees. A subset consisting of 44 of these taxa was then chosen and more rigorous analyses performed, resulting in four equally parsimonious trees. Results indicate that two major clades roughly corresponding to traditionally circumscribedAsterales andCampanulales exist as sister taxa. In particular, therbcL trees indicate thatSphenoclea is not a member ofCampanulales orAsterales, thatPentaphragma is more closely allied toAsterales thanCampanulales, that theCyphiaceae are not monophyletic, thatCampanulaceae andLobeliaceae are not sister taxa, and thatStylidiaceae are correctly placed withinCampanulales.     

New species and new combinations in Peruvian Orchidaceae

seventeen new species and combinations are proposed in the generaChondrorhyncha, Cischweinfia, Cochlioda, Eloyella, Encyclia, Kefersteinia, Koellensteinia, Macroclinium, Rodriguezia, Solenidiopsis, andStenia. All new species are illustrated. A key is provided for 2-flowered species ofMacroclinium, PeruvianSigmatostalix, and PeruvianStenia. Solenidium (Solenidiopsis) peruvianum Schltr. is lectotypified.