共查询到19条相似文献,搜索用时 140 毫秒
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对中国林蛙属动物进行了DNA水平的分子系统发生研究,测定了中国林蛙属7种15个样品,侧褶蛙属2种2个样品Cyt b基因长约360bp片段的序列。对这些序列的系统发生分析表明:(1)中国林蛙甘肃种群与东北种群序列差异较大,但尚难根据序列差异来判断它们是不同的亚种还是不同的物种;(2)7种林蛙中,中国林蛙与黑龙江林蛙的亲缘关系最近,与桓仁林蛙最远;(3)支持将昭觉林蛙,镇海林蛙,峨眉林蛙和长肢林蛙归入日本林蛙种组Rana japonica group。 相似文献
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用骨髓细胞制片法对桓仁林蛙(Rana huanrenesis)和桓仁产东北林蛙(R.dybowskii)的染色体组型进行了报道,两种林蛙的染色体数均为2n=24,都可配成12对,按照相对长度可分为3组,A组包括第1~5对,为大型染色体(相对长度〉9.0);B组是第6对,为中型染色体(相对长度在7.0~9.0之间);C组包括第7~12对,这一组为小型染色体(相对长度〈7.0)。在两种林蛙的染色体组型中未发现有异型性染色体。桓仁林蛙的第1、3、4、5对为中部着丝点染色体,第9对为端部着丝点染色体,其余各对为亚中部着丝点染色体。东北林蛙的第1、2、3、4、5、6、8对为中部着丝点染色体,第9对为端部着丝点染色体,其余各对为业中部着丝点染色体。两种林蛙的第11对染色体长臂有明显的次缢痕。 相似文献
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吉林省两栖动物一种新纪录--桓仁林蛙 总被引:4,自引:2,他引:2
2005年5月,在吉林省东部敬宇县和扶松县进行标本采集中,所采一只林蛙标本经鉴定为桓仁林蛙(Rana huanrenensis Liu,Zhang and Liu)。桓仁林蛙已知分布于辽宁省桓仁县和朝鲜半岛(费梁等,2005),因此在抚松采集到的桓仁林蛙为吉林省新纪录。现报道如下:雌蛙,体形较窄短,头宽大于头长,鼻间距大于眼间距。鼓膜较小,不足眼径之半。皮肤较光滑,体背和体侧疣少而且小。胫跗关节前达鼻孔,胫长超过体长之半。4、5趾间蹼全蹼。体背为棕褐色,颞部黑色三角斑明显,腹面咽胸部为灰色,腹部白色。 相似文献
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测定了 6种林蛙和用作外群的 2种侧褶蛙和 1种陆蛙的线粒体 12SrRNA基因序列 393bp。序列两两对位比较表明内外群间的位点替换率是 7 3%到 2 3 1% ,内群中物种间则为 0 0 %到 9 2 %。依据上述DNA序列 ,用距离法和最大简约法的系统发育分析表明 :①研究的 6种林蛙聚为一支 ,构成单系群 ,并有高的BPs值(90 %以上 )支持 ;② 6种林蛙可以分成 2个姐妹群 ,即中国林蛙、黑龙江林蛙和桓仁林蛙为一组 (BPs >94% ) ,峨眉林蛙、昭觉林蛙和镇海林蛙为一组 (BPs >5 0 % ) ;③昭觉林蛙与镇海林蛙有较近的亲缘关系 ;④中国林蛙的榆中种群与牡丹江种群间的遗传分化似乎达到了种级分化水平。 相似文献
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实验通过在设施环境下和控制条件下,研究出蛰温度、湿度及风速3种环境因素对林蛙生长和存活的影响,目的在于探索东北林蛙(Rana dybowskii)适宜的出蛰环境。结果表明:温度是影响东北林蛙出蛰存活和生长的重要因素,当出蛰温度低于16℃,东北林蛙有较高的成活率,而出蛰温度高于20℃,则会引起70%以上的东北林蛙死亡,出蛰期间温度缓慢提高有利于东北林蛙生长和存活。东北林蛙出蛰期间适宜的湿度在80%以上,湿度低于60%则对东北林蛙不利。出蛰环境以无风为好,有风会造成东北林蛙的死亡。在人工养殖过程中,可依据当地、当年的气候来进行出蛰。出蛰当日平均温度宜在15℃以下。 相似文献
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A taxonomic study of the Rana narina complex, with description of three new species (Amphibia: Ranidae) 总被引:1,自引:0,他引:1
MASAFUMI MATSUI 《Zoological Journal of the Linnean Society》1994,111(4):385-415
A morphometric and electrophoretic survey was conducted to examine taxonomic relationships among eight population samples of the Rana narina complex from the Ryukyu Archipelago of Japan and Taiwan. Five discrete morphotypes are differentiated, and these showed considerable genetic differentiation from each other. From the available information, each of the five morphotypes is judged to represent a species, and three are described as new. Rana utsunomiyaorum sp. nov. and R. supranarina sp. nov. are sympatric in Ishigakijima and Iriomotejima islands of the Yaeyama Group, and differ from the other members by having a shorter hindlimb. Rana utsunomiyaorum is the smallest in the complex, while R. supranarina is the largest. Rana amamiensis sp. nov . occurs on Amamioshima and Tokunoshima islands of the Amami Group and, like R. narina from Okinawajima of the Okinawa Group, has a long hindlimb; it differs from the latter species by having a larger body and relatively small tympanum. These two species differ from R. swinhoana from Taiwan by having a narrower disk on the third finger. A key to known species of the complex is given. Further, syslematics of the R. narina complex within Rana , body size in the two sympatric species, and sexual dimorphism found in this complex are discussed. 相似文献
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泽蛙、日本林蛙、饰纹姬蛙不同地理居群的核型多样性 总被引:5,自引:0,他引:5
本文研究了温州地区的泽蛙、日本林蛙、饰纹姬蛙的核型,并分析了9个地理居群泽蛙的核型、4个地理居群日本林蛙的核型和3个地理居群饰纹姬蛙的核型。结果表明,不同地理居群的同种蛙均有相同的染色体数和核型模式。泽蛙、日本林蛙都为 2n=26,NF=52,核模式5+8;饰纹姬蛙 2n=24,NF=48,核模式6+6。但同一种蛙的不同地理居群之间在SM数目和顺序、次缢痕或随体的位置等都有所不同,说明不同地理居群的同种蛙的染色体具有丰富的多样性。故保护蛙品种资源多样性,不仅要从整个群体上考虑,而且要针对每个品种(或类群)进行保护。
Abstract:The karyotypes of Rana limmocharis boie,Rana j.Japonica,and Microhyla ornata from Wenzhou were studied.The karyotypes of nine populations of Rana limmocharis boie,four populations of Rana j.Japonica and three populations of Microhyla ornata from different geographical regions were compared.The results demonstrated that the same species of different geographical populations have the same amount of chromosome and karyotypic formulae. Rana limmocharis boie and Rana j.Japonica have 2n=26,NF=52 and 5+8 karyotypic formulae.Microhyla ornata has 2n=24,NF=48 and 6+6 karyotypic formulae.But some dissimilarities were found among them.First,the number and sequence of submetacentric chromosome were different among them,and then the secondary contriction (SC)or satellite (Sat)were also different.It was showed that the chromosomes of same species of different geographical population have diversities.Conservation of frog genetic diversity must be considered of not only the genetic diversity conservation of the total frog population but also that of every frog breed. 相似文献
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U. Sinsch H. Schneider 《Journal of Zoological Systematics and Evolutionary Research》1996,34(2):63-73
Six structural and temporal features of the mating calls of pool frogs recorded at eight localities in Italy, Germany, Yugoslavia and Albania were compared with those of the calls of topotypical Rana lessonae in northern Italy. All populations shared three out of six characters studied: call duration, intercall interval and call period. The pool frogs of three localities in peninsular Italy significantly differed in three characters from those of the other six localities, including the northern Italian one: pulses per pulse group, pulse groups per call, and pulse groups per second. This study demonstrates the existence of Rana bergeri , a pool frog species different from R. lessonae in peninsular Italy, but does not support the species status of the chemotaxonomically established R. shqiperica , which is considered as a synonym of R. lessonae . 相似文献
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两种蛙Sox基因的PCR-SSCP分析 总被引:3,自引:1,他引:2
采用PCR技术,以参考人SRI,基因HMG-box的保守区序列而设计一对特异引物,扩增了黑斑蛙和金线蛙的Sox基因。结果两种蛙的雌雄个体均扩增出217bp的基因片段,与人对照相同。对扩增产物进行SSCP分析显示,两种蛙雌雄个体间的单链迁移率相同,两种蛙之间差异较小,而与人有较大差异。测序表明,两种蛙的Sox序列之间以及与各类物种的Sox基因都有非常高的相似性,充分显示出Sox基因在系统进化上的高度保守性。 相似文献
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U. Sinsch H. Schneider 《Journal of Zoological Systematics and Evolutionary Research》1999,37(2):67-74
Eight morphometric features of water frogs of 14 localities in Turkey, Syria, Jordan and Israel were compared with those of Rana ridibunda in Kazakhstan, Armenia and Greece (Thrace). These study sites include the type localities of R. ridibunda , R. r. caralitana , R. esculenta var. bedriagae and R. levantina . Multivariate comparisons (principal-component analysis, discriminant analysis) based on the log10 -transformed variables demonstrate that the data set includes only two taxa that differ significantly in size and shape. By applying a morphospecies criterion, R. ridibunda is represented exclusively by the three reference populations, whereas all other populations (in Turkey, Syria, Jordan and Israel) represent the same species , R. bedriagae . 相似文献