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ACC合酶基因(ACSG)可能是黄瓜雌性系的分子标记 总被引:3,自引:0,他引:3
利用本实验室根据已知序列分离得到的ACC合酶基因(ACSG)为探针对不同黄瓜(Cucumis sativus L.)品种(系)的基因组DNA进行Southern杂交,初步分析了该基因与黄瓜性别表型之间的相关性。发现在所检测的10个不同品系中,ACSG与雌性系表型之间存在明显的相关关系,而且这种相关关系在不同的实验中具有良好的重复怀。ACSG基因可能是鉴定黄瓜雌性系的一个分子标记。 相似文献
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17β-雌二醇诱导血管内皮细胞一氧化氮释放及其与细胞内钙的关系 总被引:6,自引:0,他引:6
利用小牛胸主动脉内皮细胞(BAECs)作为模型,观察17β-雌二醇(E2)BAECs一氧化氮(NO)释放、一氧化氮合酶(eNOS)mRNA表达和细胞内钙(〔Ca^2+〕i)的影响,以及雌激素受体(ER)拮抗剂tamoxifen和NOS抑制剂(L-NAME)的作用。结果显示,E2(10^-12 ̄10^-8mol/L)呈尝试依赖性促进BAECs中NO的释放,以10^-8mol/L浓度E2处理BAECs 相似文献
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亚洲棉GAE6—3A上游序列的分离及其在烟草中的表达 总被引:2,自引:0,他引:2
根据E6基因保守域设计引物,PCR扩增出亚洲棉(Gassypium arboreum L.)GAE6基因长约400bp片段,序列分析表明该片段与海棉(G.bargbadense)E6基因同源性达96.8%。进一步合成2个反向引物协助进行PCR-96孔板筛库分离到亚洲板棉GAE6-3A克隆。酶切鉴定其插入片段长约8.0kb,序列测定及分析结果表明其上游和约1.5kb,将GAE6-3A上游序列克 含有 相似文献
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水稻核糖核蛋白基因片段的结构分析与鉴定 总被引:1,自引:0,他引:1
利用两个源自于核糖核蛋白(RNP)一致顺序Ⅰ和一致顺序Ⅱ氨基酸顺序的寡核苷酸片段作PCR引物,从水稻(Oryza sativa L.)“广陆矮4号”黄化苗cDNA 中扩增到了一个300 bp长的基因片段.顺序分析显示这个基因片段具有典型的RNP蛋白特征,即两个含RNP一致顺序的RNA 结合区域 相似文献
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苏云金芽孢杆菌毒蛋白基因在小麦基因组中的甲基化修饰 总被引:8,自引:1,他引:7
通过花粉管通道法将苏云金芽孢杆菌(Bacillusthuringiensis)毒蛋白基因(Bt.toxingene)转进了小麦品种京花5号与86Al。利用点杂交、Southern杂交和PCR等技术鉴定了转化植株的当代和子代,证实了Bttoxingene的导入与对小麦染色体的整合。利用对腺嘌呤(A)与胞嘧啶(C)甲基化敏感与否的限制性内切酶组(medhylation-(un)sensitiverestrictionenzymegroup,MREG)酶切和RCR扩增(MREG-PCR),对转化子代中的Bt基因片段甲基化形式进行了研究。结果表明,整合在小麦基因组中的Bt基因的A和C的甲基化形式发生了变化 相似文献
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中华姬鼠种群生态的初步分析THEPRELIMINARYANALYSISONTHEPOPULATIONECOLOGYOFCHINESEFIELDMOUSE(APODEMUSDRACO)KeyWordsChineseFieldMouse(Apodemus... 相似文献
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本研究观察了低氧对大鼠肺组织和血管内皮一氧化氮合酶(NOS)活性及内皮衍生一氧化氮(EDNO)依赖性舒张反应的影响,以及NOS抑制剂(L-NAME)对常氧和低氧大鼠肺组织和血管内皮NOS活性及颈、肺动脉血压(CAPs、mPAP)的作用。结果表明常氧大鼠肺泡内无肌性血管内皮未见NOS活性,其肺血管床对EDNO依赖性舒血管物质BK没有反应,注射L-NAME后大鼠mPAP略有降低,CAPs有所升高。低氧大鼠肺泡内无肌性血管内皮显示NOS活性,对BK的EDNO依赖性舒张反应呈剂量依赖性增大,注射L-NAME使低氧大鼠mPAP显著降低(P<0.01),CAPs显著升高(P<0.05)。提示肺血管EDNO及其合酶在维持正常成年大鼠肺循环低压低阻中的生理作用值得进一步探讨;低氧引起肺血管内皮ecNOS活性增加和EDNO生成增多可能起到限制肺动脉压过度升高的调制作用,也可能对肺血管内皮产生毒性作用,反而促进肺动脉高压的发生和发展。 相似文献
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黑龙江铜木蚜蝇雌性的描述DESCRIPTIONOFTHEFEMALECHALCOSYRPHUS(XYLOTOMIMA)AMURENSIS(STACKELBERG)(DIPTERA:SYRPHIDAE)¥HEJilong;CHUXiping(Depar... 相似文献
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正Dear Editor,In December 2019, a novel human coronavirus caused an epidemic of severe pneumonia(Coronavirus Disease 2019,COVID-19) in Wuhan, Hubei, China(Wu et al. 2020; Zhu et al. 2020). So far, this virus has spread to all areas of China and even to other countries. The epidemic has caused 67,102 confirmed infections with 1526 fatal cases 相似文献
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Curcumin is the yellow pigment of turmeric that interacts irreversibly forming an adduct with thioredoxin reductase (TrxR), an enzyme
responsible for redox control of cell and defence against oxidative stress. Docking at both the active sites of TrxR was performed to compare
the potency of three naturally occurring curcuminoids, namely curcumin, demethoxy curcumin and bis-demethoxy curcumin. Results show
that active sites of TrxR occur at the junction of E and F chains. Volume and area of both cavities is predicted. It has been concluded by
distance mapping of the most active conformations that Se atom of catalytic residue SeCYS498, is at a distance of 3.56 from C13 of
demethoxy curcumin at the E chain active site, whereas C13 carbon atom forms adduct with Se atom of SeCys 498. We report that at least
one methoxy group in curcuminoids is necessary for interation with catalytic residues of thioredoxin. Pharmacophore of both active sites of
the TrxR receptor for curcumin and demethoxy curcumin molecules has been drawn and proposed for design and synthesis of most probable
potent antiproliferative synthetic drugs. 相似文献
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The young pistils in the melanthioid tribes, Hewardieae, Petrosavieae and Tricyrteae, are uniformly tricarpellate and syncarpous. They lack raphide idioblasts. All are multiovulate, with bitegmic ovules. The Petrosavieae are marked by the presence of septal glands and incomplete syncarpy. Tepals and stamens adhere to the ovary in the Hewardieae and the Petrosavieae but not in the Tricyrteae. Two vascular bundles occur in the stamens of the Hewartlieae and Tricyrtis latifolia. Ventral bundles in the upper part of the ovary of the Hewardieae are continuous with compound septal bundles and placental bundles in the lower part. Putative ventral bundles occur in the alternate position in the Tricyrteae and putative placental bundles in the opposite. position in the Petrosavieae. The dichtomously branched stigma in each carpel of the Tricyrteae is supplied by a bifurcated dorsal bundle. 相似文献
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Meng Miao Gang Deng Xiaobei Xiong Yang Qiu Wenda Huang Meng Yuan Fei Yu Shimei Bai Xi Zhou Xiaolu Zhao 《中国病毒学》2022,37(2):314-317
Highlights
1. The N-terminal tail of histone H3 is specifically cleaved during EV71 infection.
2. Viral protease 3C is identified as a protease responsible for proteolytically processing the N-terminal H3 tail.
3. Our finding reveals a new epigenetic regulatory mechanism for Enterovirus 71 in virus-host interactions. 相似文献
1. The N-terminal tail of histone H3 is specifically cleaved during EV71 infection.
2. Viral protease 3C is identified as a protease responsible for proteolytically processing the N-terminal H3 tail.
3. Our finding reveals a new epigenetic regulatory mechanism for Enterovirus 71 in virus-host interactions. 相似文献
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Dong Liu Xin Wang Yisong Wang Peigang Wang Dongying Fan Sichang Chen Yuguang Guan Tianfu Li Jing An Guoming Luan 《中国病毒学》2018,33(5):402-409
Rasmussen’s encephalitis (RE) is a rare pediatric neurological disorder, and the exact etiology is not clear. Viral infection may be involved in the pathogenesis of RE, but conflicting results have reported. In this study, we evaluated the expression of both Epstein-Barr virus (EBV) and human herpes virus (HHV) 6 antigens in brain sections from 30 patients with RE and 16 control individuals by immunohistochemistry. In the RE group, EBV and HHV6 antigens were detected in 56.7% (17/30) and 50% (15/30) of individuals, respectively. In contrast, no detectable EBV and HHV6 antigen expression was found in brain tissues of the control group. The co-expression of EBV and HHV6 was detected in 20.0% (6/30) of individuals. In particular, a 4-year-old boy had a typical clinical course, including a medical history of viral encephalitis, intractable epilepsy, and hemispheric atrophy. The co-expression of EBV and HHV6 was detected in neurons and astrocytes in the brain tissue, accompanied by a high frequency of CD8+ T cells. Our results suggest that EBV and HHV6 infection and the activation of CD8+ T cells are involved in the pathogenesis of RE. 相似文献