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1.  新疆出血热病毒糖蛋白基因的克隆与表达  
   马本江  杭长寿  赵云  王世文  解燕乡《病毒学报》,2002年第18卷第3期
   On the basis of sequencing and analyzing of the whole M genes(encoding viral membrane antigen glycoprotein) of three Crimean Congo hemorrhagic fever viruses(CCHFV),the Chinese isolates(Xinjiang hemorrhagic fever virus,XHFV),we first expressed the glycoprotein (GP) gene of the prototype human origin XHFV strain BA66019 in eukaryotic cells and investigated the expression profiles.Three eukaryotic expression plasmids were constructed starting from ATG 78 (the first start codon of the deduced entire XHFv GP precursor gene positioned between the 78 th ~80 th nucleotides),ATG 93 and ATG 3084 (the potential start codon for G1 precursor gene).The constructs were transfected to COS-7 cells and the expressed products were characterized as membrane-bound proteins which could induce cell fusion This was much more apparent for recombinant plasmid with ATG 3084 A recombinant baculovirus was further created harboring full length GP gene(starting from ATG 78 ) and the expression could also result in the membrane fusion as well as swelling of the infected Sf9 cells The insect cell expressed G1 was smaller in M W than natural G1 (approximately 67kD) on SDS-PAGE and no recombinant G2 band was detectable Western-blot only detected the native G1,while there was no specific corresponding band of recombinant G1 These data suggested that G1 was structurally and functionally important in XHFV and the glycosylation may have great influence on M W as well as antigenicity This study provides a foundation for the future study of viral pathogenesis ,antigenicity and immunity,that are the theoretical and experimental backgrounds for vaccine development    

2.  风疹病毒JR23株包膜糖蛋白E2重组体的构建表达与抗原性分析  
   王小凡 王志玉 薛永磊 姚苹《Virologica Sinica》,2003年第18卷第5期
   To construct the recombinant plasmid containing envelope glycoprotein E2 gene of RV JR23 strain, to express E2 protein in BHK21 cells, and to analyze the antigenic sites of E2 protein of RV JR23 strain, E2 gene of RV JR23 strain was amplified by RT-PCR. The PCR product was cloned into the expression vector pBluescript II SK and transformed into E. coli JM109, and the recombinant plasmid was transfected into BHK21 cells with recombinant vaccinia virus VTF-7. The expression was detected by immunohistochemistry and indirect immunofluorescence. The recombinant plasmid contained glycoprotein E2 gene, and expressed well in BHK21 cells. The expressed E2 protein mainly accumulated in plasma near the nuclei. There are immunological epitopes of E2 protein on RV JR23 virions. The results showed that RV JR23 E2 transient expression system was successfully constructed.This study offers the basis for investigation of the relationship between the structure and function of RV proteins, and interaction of virus and host cells.    

3.  风疹病毒JR23株E1包膜糖蛋白基因的表达分析  被引次数:3
   王志玉  薛永磊  王小凡  王桂亭  宋艳艳《病毒学报》,2003年第19卷第3期
   To construct the eukaryot ic expression system of E1 glycopr otein of rubella virus(RV)JR23 str ain and to study the biological an d immunological properties of the expressed product,the E1 gene was amplified by RT-PCR,sequenced and recombinated with the pBluescript ⅡSK+ vectorThe recombinated vecto r was co-transfected into BHK21 ce ll with vaccinia virus which carri es the gene of T7 RNA polymeraseT he expressed protein was characteri zed with hemadsorption,immunohisto chemistry and indirect immunofluor escence stainingThe results showe d that the recombinated vector,pBS K+-RV E1,was proved containing E1 gene by enzyme digestion and seque ncingThe expressed protein could be detected both in cytoplasm amd on membrane of the transfected cel ls by hemadsorption,immunohistoche mistry and indirect immunofluoresc enceThe E1 protein mainly focused in cytoplasm near the nucleusThes e data proved that the expression vector,pBSK+-RV E1,was successfull y constructed and the glycoprotein E1 of RV JR23 strain was effective ly expressed in BHK21 cell culture This study provides foundations f or studies on the relationships be tween structure and function of RV gene,between structure and functio n of RV proteins,and on the subuni t vaccine of rubella virus    

4.  Construction of a host range-expanded hybrid baculovirus of BmNPV and AcNPV, and knockout of cysteinase gene for more efficient expression  被引次数:4
   WU Xiaofeng  CAO Cuiping  XU Yaxiang & LU Xingmeng College of Animal Sciences  Zhejiang University  Huajiachi Campus  Hangzhou 310029  China《Science China. Life sciences》,2004年第47卷第5期
   The four main gene expression systems currently used to produce recombinant proteins are the prokary-otic, yeast, insect cell, and mammalian cell expression systems. The baculovirus expression vector system (BEVS) is a protein production system which uses a recombinant baculovirus harboring a foreign gene of interest to produce recombinant protein in an insect or its cultured cells. BEVS has many advantages: (i) BEVS requires less time to establish the production system than is needed in a…    

5.  Construction and Co-expression of Grass Carp Reovirus VP6 Protein and Enhanced Green Fluorescence Protein in the Insect Cells  被引次数:3
   Qin FANG Eng Khuan Seng Wen DAI Lan-lan ZHANG《Virologica Sinica》,2007年第22卷第5期
   Grass carp reovirus(GCRV),a disaster agent to aquatic animals,belongs to Genus Aquareovirus of family Reoviridea.Sequence analysis revealed GCRV genome segment 8(s8) was 1 296 bp nucleotides in length encoding an inner capsid protein VP6 of about 43kDa.To obtain in vitro non-fusion expression of a GCRV VP6 protein containing a molecular of fluorescence reporter,the recombinant baculovirus,which contained the GCRVs8 and eGFP(enhanced green fluorescence protein) genes,was constructed by using the Bac-to-Bac insect expression system.In this study,the whole GCRVs8 and eGFP genes,amplified by PCR,were constructed into a pFastBacDual vector under polyhedron(PH) and p10 promoters,respectively.The constructed dual recombinant plasmid(pFbDGCRVs8/eGFP) was transformed into DH10Bac cells to obtain recombinant Bacmid(AcGCRVs8/eGFP) by transposition.Finally,the recombinant bacluovirus(vAcGCRVs8/eGFP) was obtained from transfected Sf9 insect cells.The green fluorescence that was expressed by transfected Sf9 cells was initially observed 3 days post transfection,and gradually enhanced and extended around 5 days culture in P1(Passage1) stock.The stable high level expression of recombinant protein was observed in P2 and subsequent passage budding virus(BV) stock.Additionally,PCR amplification from P1 and amplified P2 BV stock further confirmed the validity of the dual-recombinant baculovirus.Our results provide a foundation for expression and assembly of the GCRV structural protein in vitro.    

6.  A NEW CELL CLONE DERIVED FROM TRICHOPLUSIA NI TN5B1-4 CELLS  
   Jian-xiaoTian Chang-youLi Gui-lingZheng Guo-xunLi PingWang Granados《Entomologia Sinica》,2004年第11卷第3期
   The characteristics of a cultured cell line do not always remain stable and may change upon continuous passage. Most continuous cell lines, even after cloning, possess several genotypes that are constantly changing. There are numerous selective and adaptive culture processes, in addition to genetic instability, that may improve phenotypic change in cell growth, virus susceptibility, gene expression, and production of virus. Similar detrimental effects of long term passaging of insect cells have also been reported for continuous cell lines, for example, Tn5B 1-4 cells, which are the most widely used for the baculovirus expression vector system (BEVS), provide superior production of recombinant proteins,however, this high productivity may be more evident in low passage cells. In this paper, we describe the isolation of a cell clone, Tn5B-40, from low passage Tn5B 1-4 cells. The growth characteristics,productions of virus, and high level of recombinant protein productions were determined. The results showed the susceptibility of both clone and Tn5B 1-4 cells to wild-type AcNPV was approximately the same rate with over 95% of infection; when the cloned cells were infected with recombinant baculoviruses expressing β-galactosidase and secreted alkaline phosphatase (SEAP), expression of the recombinant proteins from the cloned cells exceeded that from the parental Tn5B 1-4 cells.    

7.  Baculoviruses as Vectors in Mammalian Cells  被引次数:2
   Chang-yong LIANG and Xin-wen CHEN** 《Virologica Sinica》,2007年第22卷第2期
   The Baculoviridae are a large family of enveloped DNA viruses exclusively pathogenic to arthropods. Baculoviruses have been extensively used in insect cell-based recombinant protein expression system and as biological pesticides. They have been deomostrated to be safe to mammals, birds and fish. Recently, baculoviruses has been shown to transduce different mammalian cells in spite of the fact that they cannot replicate in mammalian cells (11, 73, 76). This has resulted in the development of baculoviruses as mammalian expression systems and even as vestors for gene therapy.    

8.  H9N2亚型禽流感病毒非结构蛋白(NS1)基因的克隆与表达  
   刘金华 吴清民 史为民 郭玉璞《Virologica Sinica》,2003年第18卷第5期
   The nostructural protein (NS1) encoded by gene 8 of the Influenza virus is present in cells infected with Influenza virus. In this study, NS1 protein gene of the Chicken influenza virus A/chicken/Beijing/2/97 (H9N2) strain was amplified by PCR. The fragment contains EcoR Ⅰ and Xho Ⅰ restriction enzyme sites at the ends. The amplified product was cloned into the expression vector pET-30(c). Recombinant plasmid pET/NS 1 was transformed into E.coli BL21 (DE3) competent cells and induced with 0.4 mmol/L IPTG the target protein was produced, the molecular weight of the expressed protein was 30 kDa as expected. Western-blot test indicated that the expressed protein can react with the NS 1 monoclonal antibody of the influenza virus. This study laid an important foundation for H9N2 subtype avian influenza surveillance in China.    

9.  猪细小病毒VP2蛋白主要抗原表位基因真核表达载体的构建及表达  
   任雪枫 胡志华 谈国蕾 周斌 徐学清 郑其升 张晓勇 陈德胜 陈溥言《Virologica Sinica》,2004年第19卷第6期
   Using a pair of specific primers designed according to the relevant nucleotide sequences from GenBank, the main antigen domain for VP2 gene of Porcine parvovirus was ampilified with PCR method using the genomic DNA as template. The PCR product was cloned into the expression vector pIREShyg to get a recombinant eukaryotic expression plasmid pIREShyg-VP2, which was then transfected into the CHO-K1 cells. The expressed product was detected by IFA after the positive cell clone was selected with hygromycin. The result revealed that the main antigen domain for VP2 gene of porcine parvovirus was stably expressed in CHO-K1 cells.    

10.  中国株HIV-1外膜蛋白真核表达载体的构建与表达  
   王福祥 孙永涛 王临旭 王久平 王平忠 白雪帆 黄长形《Virologica Sinica》,2004年第19卷第1期
   To construct the eukaryotic expression vector of HIV-1 gp120 gene and observe its expression in vitro, the recombinant expression vector pVAX1GP120 was constructed by inserting the gp120 gene into the eukaryotic expression vector pVAX1. The pVAX1GP120 was transfected into Vero cells by lipofectamine and the expressed product was detected by indirect immunofluore- scence.Restriction enzymes digestion analysis and sequencing results revealed that the recombinant expression vector pVAX1GP120 has been constructed successfully. The indirect immunofluorescence result showed green fluorescence on the membrane of transfected cells. The constructed eukaryotic expression vector of HIV-1 gp120 can be expressed in vitro, which lay the foundation for the further study of HIV-1 DNA vaccine.    

11.  A Mathematical Model of Baculovirus Infection on Insect Cells at Low Multiplicity of Infection  被引次数:3
   Zhang YH  Merchuk JC《Acta biochimica et biophysica Sinica》,2004年第36卷第11期
   The expression efficiency of the insect cells-baculovirus system used for insecticidal virus production and the expression of medically useful foreign genes is closely related with the dynamics of infection. The present studies develop a model of the dynamic process of insect cell infection with baculovirus at low multiplicity of    

12.  High-level expression of whiG——A key gene for Streptomyces differentiation in Escherichia coli  
   谭华荣  田宇清  杨海花  吴畏  董可宁  K. F. Chater and M. J. Buttner《Science China. Life sciences》,1996年第39卷第3期
   Six nucleotides located in the region of translation start site of whiG were changed. whiG was amplified by PCR technique. Reformed sequences were determined. This gene was directly subcloned into expression vector pET11c containing strong T7 promoter, and the recombinant plasmid was introduced into E. coli BL21(DE3), which could be induced by IPTG to produce T7 RNA polymerase. The SDS-PAGE result showed that whiG highly expressed in E. coli BL21(DE3), and the yield of whiG product was about 20% of insoluble proteins in cell. whiG product (σwhiG) was further identified by Western blot hybridization after making its antibody. whiG gene was subcloned into Streptomyces plasmid pIJ6021, and then it was introduced into sporulation deficient mutant C71 from Streptomyces coelicolor. The result showed that C71 could restore sporulation and σwhiG has biological functions.    

13.  Insect Cell Culture and Biotechnology  
   Robert R.Granados  G.W.Blissard1《Virologica Sinica》,2007年第22卷第2期
   The continued development of new cell culture technology is essential for the future growth and application of insect cell and baculovirus biotechnology. The use of cell lines for academic research and for commercial applications is currently dominated by two cell lines; the Spodoptera frugiperda line, SF21 (and its clonal isolate, SF9), and the Trichoplusia ni line, BTI 5B1-4, commercially known as High Five cells. The long perceived prediction that the immense potential application of the baculovirus-insect cell system, as a tool in cell and molecular biology, agriculture, and animal health, has been achieved. The versatility and recent applications of this popular expression system has been demonstrated by both academia and industry and it is clear that this cell-based system has been widely accepted for biotechnological applications. Numerous small to midsize startup biotechnology companies in North America and the Europe are currently using the baculovirus-insect cell technology to produce custom recombinant proteins for research and commercial applications. The recent breakthroughs using the baculovirus-insect cell-based system for the development of several commercial products that will impact animal and human health will further enhance interest in this technology by pharma. Clearly, future progress in novel cell and engineering advances will lead to fundamental scientific discoveries and serve to enhance the utility and applications of this baculovirus-insect cell system.    

14.  Construction of Prophylactic Human Papillomavirus Type 16 L1 Capsid Protein Vaccine Delivered by Live Attenuated Shigellaflexneri Strain sh42  
   Yang XF  Qu XZ  Wang K  Zheng J  Si LS  Dong XP  Wang YL《Acta biochimica et biophysica Sinica》,2005年第37卷第11期
   To express human papillomavirus (HPV) L 1 capsid protein in the recombinant strain of Shigella and study the potential of a live attenuated Shigella-based HPV prophylactic vaccine in preventing HPV infection, the icsA/virG fragment of Shigella-based prokaryotic expression plasmid pHS3199 was constructed. HPV type 16 L1 (HPV16L1) gene was inserted into plasmid pHS3199 to form the pHS3199-HPV16L1 construct, and pHS3199-HPV 16L1 was electroporated into a live attenuated Shigella strain sh42. Western blotting analysis showed that HPV 16L 1 could be expressed stably in the recombinant strain sh42-HPV 16L 1. Sereny test results were negative, which showed that the sh42-HPV16L1 lost virulence. However, the attenuated recombinant strain partially maintained the invasive property as indicated by the HeLa cell infection assay. Specific IgG, IgA antibody against HPV16L1 virus-like particles (VLPs) were detected in the sera, intestinal lavage and vaginal lavage from animals immunized by sh42-HPV 16L1. The number of antibodysecreting cells in the spleen and draining lymph nodes were increased significantly compared with the control group. Sera from immunized animals inhibited mufine hemagglutination induced by HPV 16L1 VLPs, which indicated that the candidate vaccine could stimulate an efficient immune response in guinea pig's mucosal sites. This may be an effective strategy for the development of an HPV prophylactic oral vaccine.    

15.  Co-expression and Immunity of Legionella pneumophila mip Gene and Immunoadjuvant ctxB Gene  被引次数:1
   Wang T  Chen JP  Li H  Zhi KQ  Zhang L  Yang CL  Tao DC《Acta biochimica et biophysica Sinica》,2005年第37卷第3期
   The mip gene of Legionella pneumophila and the ctxB gene of Vibrio cholerae were amplifiedby PCR respectively.The amplified cDNA was ligated to the pcDNA3.1(+)vector.The recombinant plasmidspcDNA3,1-mip and pcDNA3.1-ctxB were identified by restriction analysis and PCR,and further confirmedby sequencing analysis.NIH3T3 cells were transfected with pcDNA3.1-mip and pcDNA3.1-ctxB accordingto the Lipofection method.Transient and stable products of the co-expression of the mip gene and ctxB genewere detected by immunofluorescence and Western blotting.The results showed that NIH3T3 cells weresuccessfully transfected,and that the transiently and stably co-expressed products can be detected in thetransfected cells.To detect the humoral and cellular immune response in immunized mice induced by the co-immunization of the mip and ctxB genes,female BALB/c mice were immunized intramuscularly with pcDNA3.1-mip and pcDNA3.1-ctxB.The results showed that the specific antibody titer and the cytotoxic T-lymphocyteresponse for pcDNA3,1-mip immunization and co-immunization were increased compared with that ofpcDNA3.1(+) immunization.Furthermore,the specific antibody titer and cytotoxic T-lymphocyte responsefor co-immunization were increased compared with that of pcDNA3.1-mip immunization.Statistical analysisusing one-way analysis of variance(ANOVA)showed that there was a significant difference between thegroups(P<0.01).The results indicated that the ctxB gene enhanced the humoral and cellular immune responseto the mip gene immunization.These findings provide experimental evidence to support the development ofthe L.pneumophila DNA vaccine.    

16.  Characterization of a Mutant Listeria monocytogenes Strain Expressing Green Fluorescent Protein  被引次数:2
   Jiang LL  Song HH  Chen XY  Ke CL  Xu JJ  Chen N  Fang WH《Acta biochimica et biophysica Sinica》,2005年第37卷第1期
   To construct a recombinant strain of Listeria monocytogenes for the expression of heterologous genes, homologous recombination was utilized for insertional mutation, targeting its listeriolysin O gene (hly). The gene encoding green fluorescent protein (GFP) was used as the indicator of heterologous gene expression. The gene gfp was inserted into hly downstream from its promoter and signal sequence by an overlapping extension polymerase chain reaction, and was then cloned into the shuttle plasmid pKSV7 for allelic exchange with the L. monocytogenes chromosome. Homologous recombination was achieved by growing the electro-transformed L. monocytogenes cells on chloramphenicol plates at a non-permissive temperature. Sequencing analysis indicated correct insertion of the target gene in-frame with the signal sequence. The recombinant strain expressed GFP constitutively as revealed by fluorescence microscopy. The mutant strain L. monocytogenes hly-gfp lost its hemolytic activity as visualized on the blood agar or when analyzed with the culture supernatant samples. Such insertional mutation resulted in a reduced virulence of about 2 logs less than its parent strain L. monocytogenes 10403s as shown by the 50%-lethal-dose assays in the mouse and embryonated chicken egg models. These results thus demonstrate that mutated L. monocytogeues could be a potential carrier for the expression of heterologous passenger genes or could act as an indicator organism in the food industry.    

17.  铜绿蝇蜕皮激素受体在杆状病毒载体系统中的表达  
   代小江  Huang Q Sue  庞义《病毒学报》,2000年第16卷第2期
   The baculovirus expression vector, Trichoplusia ni nucleopolyhedrovirus, with the advantage of polyhedral inclusion body formation in recombinant viruses, was used to express the ecdysteroid receptor of the Australian sheep blowfly Lucilia cuprina(LcEcR). pSXIVVI +X3/2 baculovirus transfer vector was chosen for a 2.8kb LcEcR cDNA subcloning since pSXIVVI +X3/2 contains an efficient translational initiation signal (ATG) and it allows the LcEcR cDNA fusion to N-terminal codons in the correct reading frame. The resulting transfer plasmid pSXIVVI +X3-LcEcR was cotransfected into BT1-Tn-5B1-4 cells with the parental virus TnNPV-SVI -G minus polyhedral inclusion body, which expresses β-galactosidase gene. After 3~4 runs of plaque purification, three TnNPV-LcEcR clones were obtained with the LcEcR gene under the dual control of synthetic and XIV promoters. These three TnNPV-LcEcR clones all showed white phenotype when stained with X-gal. Western blot analysis showed 2~3 specific polypeptides with molecular weight ranging from 70~90kD. Three TnNPV-LcEcR clones expressed different level of LcEcR polypeptides in BT1-Tn-5B1-4 cells. The TnNPV-LcEcR-1 clone expressed the highest level of LcEcR polypeptides in BT1-Tn-5B1-4 cells 48~72h post infection.    

18.  Expression and Antigenic Characterization of the Epitope-G1 of the Bovine Ephemeral Fever Virus Glycoprotein in Pichia pastoris  
   Fu-ying ZHENG Guo-zhen LIN Chang-qing QIU Kui-zhang YUAN Jun-ying SONG《Virologica Sinica》,2007年第22卷第5期
   The epitope-G1 gene of Bovine ephemeral fever virus(BEFV) glycoprotein was synthesised by PCR and cloned into expression vector pPIC9K to construct recombinant plasmid pPIC9K-G1.Then the pPIC9K-G1 was linearized and transformed into Pichia pastoris GS115.The recombinant P.pastoris strains were selected by a G418 transformation screen and confirmed by PCR.After being induced with methanol,an expressed protein with 26 kDa molecular weight was obtained,which was much bigger than the predicted size(15.54 kDa).Deglycosylation analysis indicated the recombinant G1 was glycosylated.Western blot and ELISA tests,as well as rabbit immunization and specificity experiments indicated that the target protein had both higher reaction activity and higher immunocompetence and specificity.The recombinant G1 protein could be used as a coating antigen to develop an ELISA kit for bovine ephemeral fever diagnosis.    

19.  Expression in Escherichia coli of Three Different Soybean Late Embryogenesis Abundant (LEA) Genes to Investigate Enhanced Stress Tolerance  被引次数:1
   YingLAN DanCAI Yi-ZhiZHENG《Journal of Integrative Plant Biology》,2005年第47卷第5期
   In order to identify the function of late embryogenesis abundant (LEA) genes, in vitro functional analyses were performed using an Escherichia coli heterologous expression system. Three soybean late embryogenesis abundant (LEA) genes, PMll (GenBank accession No. AF004805; group 1), PM30(AF117884; group 3), and ZLDE-2 (AY351918; group 2), were cloned and expressed in a pET-28a system.The gene products were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and identified by mass spectrometry. E. coli cells containing the recombinant plasmids or empty vector as controls were treated by salt and low temperature stress. Compared with control cells, the E. coli cells expressing either PMll or PM30 showed a shorter lag period and improved growth when transferred to LB (Luria-Bertani) liquid media containing 800 mmol/L NaC1 or 700 mmol/L KC1 or after 4℃ treatment. E. coli cells expressing ZLDE-2 did not show obvious growth improvement both in either high KC1 medium or after 4℃ treatment. The results indicate that the E. coli expression system is a simple, useful method to identify the functions of some stress-tolerant genes from plants.    

20.  Construction and characterization of a recombinant fowlpox virus containing HIV-1 multi-epitope-p24 chimeric gene in mice  被引次数:1
   LiShu Zhang  NingYi Jin  YingJin Song  Hong Wang  HeWen Ma  ZiJian Li  WenZheng Jiang《Science China. Life sciences》,2007年第50卷第2期
   The epidemic of HIV/AIDS is sweeping across the world. It is of great importance to figure out new ways to curb this disease. Epitope-based vaccine is one of these solutions. In this study, a chimeric gene was obtained by combination of a designed HIV-1 multi-epitope gene (MEG) and HIV-1 p24 gene. A re- combinant plasmid pUTA2-MEGp24 was then constructed by inserting MEGp24 gene into the down- stream of the promoter (ATI-P7.5×20) of fowlpox virus (FPV) transfer vector pUTA2. The recombinant plasmid and wild-type FPV 282E4 strain were then co-transfected into CEF cells and homologous re- combination occurred. A recombinant virus expressing HIV-1 protein MEGp24 was screened by ge- nome PCR and Western blot assay. Large scale preparation and purification of the recombinant fowl- pox virus (rFPV) were then carried out. BALB/c mice were immunized intramuscularly with the rFPV for three times on day 0, 14 and 42. Mice were executed and sampled one week after the third inoculation. Anti-HIV-1 antibody in serum and Th1 cytokines in the supernatant of cultured spleen cells were as- sayed by ELISA. The count of T lymphocyte subsets and the CTL activity of spleen lymphocytes were analyzed by flow cytometry and lactate dehydrogenase (LDH) release assay, respectively. The results showed that HIV-1 specific antibody in serum and increased T lymphocyte subsets (CD4 T, CD8 T) were detected in the immunization group. CTL target-killing activity and higher secretion of Th1 cyto- kines (IFN-γ and IL-2) of spleen lymphocytes stimulated by H-2d-restricted CTL peptide were observed in immunized mice. We concluded that the rFPV may induce HIV-1 specific immunity especially cellular immunity in mice.    

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