A taxonomic review of Korean Hydroglyphus Motschulsky (Coleoptera: Dytiscidae: Hydroporinae) with a description of new species

A taxonomic study of Hydroglyphus Motschulsky in Korea is presented. Four species are recognized including a new species, Hydroglyphus coreanus sp. nov. Hydroglyphus flammulatus (Sharp) is reported for the first time in the Korean peninsula and H. geminus (Fabricius) is a new addition to the South Korean fauna. Habitus and SEM photographs, distribution maps, and key to the Korean species are provided.     

Taxonomic review of the Haliplidae (Coleoptera) in the Korean peninsula

A taxonomic review of Korean Haliplidae Aubé is presented. Eight species in two genera are recognized, one of which (Haliplus diruptus Balfour‐Browne) is reported for the first time in South Korea. We also found that H. ovalis Sharp previously recorded in the Korean peninsula was an incorrect identification of H. chinensis Falkenström. Habitus and scanning electron microscopy (SEM) photographs, diagnoses of genera, additional characters of species and diagnostic characters with illustrations of the species are provided.     

CAPS markers using mitochondrial consensus primers for molecular identification of Panax species and Korean ginseng cultivars (Panax ginseng C. A. Meyer)

Cleaved amplified polymorphic sequence (CAPS) marker system using mitochondrial consensus primers was applied for molecular identification of Korean ginseng cultivars (Panax ginseng). Initially, a total of 34 primers were tested to six Korean ginseng cultivars and two foreign Panax species, P. quinquefolius and P. notoginseng. In the polymerase chain reaction (PCR) amplification results, four primers (mt7, mt11, mt13, and mt18) generated co-dominant polymorphic banding patterns discriminating the Korean ginseng cultivars from P. quinquefolius and P. notoginseng. In the CAPS analysis results, the majority of the cleaved PCR products also yielded additional latent polymorphisms between the Korean ginseng cultivars and two foreign Panax species. Specific latent CAPS polymorphisms for cultivar Gopoong and Chunpoong were detected from internal region amplified with mt9 primer by treating HinfI and Tsp509I endonucleases, respectively. Sequencing analysis revealed that the length of amplified region of Korean ginseng cultivars was 2,179 bp, and those of P. quinquefolius and P. notoginseng were 2,178 and 2,185 bp, respectively. Blast search revealed that the amplified region was a mitochondrial cytochrome oxidase subunit 2 (cox2) gene intron II region. Nineteen single nucleotide polymorphisms (SNP) including each specific SNP for Gopoong and Chunpoong, and three insertion and deletion (InDel) polymorphisms were detected by sequence alignment. The CAPS markers developed in this study, which are specific to Gopoong and Chunpoong, and between the Korean ginseng cultivars and two foreign Panax species, will serve as a practical and reliable tool for their identification, purity maintenance, and selection of candidate lines and cultivars.     

Revision of the family Sphecidae (Hymenoptera: Apoidea) in South Korea

Korean species of the family Sphecidae are reviewed, and seventeen species in nine genera belonging to three subfamilies are confirmed. Among them, Ammophila campestris and Sceliphron caementarium are new to South Korea. Korean occurrence of Sceliphron madraspatanum is confirmed with an actual Korean specimen. This paper provides the first determination keys to higher taxa and species occurring in South Korea. Taxonomic information of each species including original publication of valid name and justifiable references for Korean occurrence is provided. Digital images and line drawings for selective characteristics are also available.     

Rapid molecular authentication of three medicinal plant species, Cynanchum wilfordii, Cynanchum auriculatum, and Polygonum multiflorum (Fallopia multiflorum), by the development of RAPD-derived SCAR markers and multiplex-PCR

Definitive identification of original plant species is important for standardizing herbal medicine. The herbal medicines Cynanchi Wilfordii Radix (Baekshuoh in Korean and Beishuwu in Chinese) and Polygoni Multiflori Radix (Hashuoh in Korean and Heshuwu in Chinese) are often misidentified in the Korean herbal market due to morphological similarities and similar names. Therefore, we developed a reliable molecular marker for the identification of Cynanchi Wilfordii Radix and Polygoni Multiflori Radix. We used random amplified polymorphic DNA (RAPD) analysis of three plant species, Polygoni multiflorum, Cynanchum wilfordii, and Cynanchum auriculatum, to obtain several species-specific RAPD amplicons. From nucleotide sequences of these RAPD amplicons, we developed six sequence characterized amplification region (SCAR) markers for distinguishing Polygoni Multiflori Radix and Cynanchi Wilfordii Radix. Furthermore, we established SCAR markers for the simultaneous discrimination of the three species within a single reaction by using multiplex-PCR. These SCAR markers can be used for efficient and rapid authentication of these closely related species, and will be useful for preventing the distribution of adulterants.     

Systematic Rearrangement of Korean <Emphasis Type="Italic">Scirpus</Emphasis> L. s.l. (Cyperaceae) as Inferred from Nuclear ITS and Chloroplast <Emphasis Type="Italic">rbcL</Emphasis> Sequences

The genus Scirpus L. s.l. (Cyperaceae) has been accepted as a polyphyletic taxon by most plant taxonomists. This genus was separated into different genera by several different authors: Scirpus s. str., Trichophorum Pers., Bolboschoenus (Asch.) Palla, Schoenoplectus (Rchb.) Palla, and Schoenoplectiella Lye. The heterogeneity of Korean Scirpus s.l. has not yet been studied. We examined 17 taxa of Korean Scirpus s.l. by morphological characters and phylogenetic analyses based on nuclear ITS and chloroplast rbcL sequences. Phylogenetic analyses using maximum parsimony, maximum likelihood, and Bayesian method provided sufficient resolution. The phylogeny revealed the polyphyly of Korean Scirpus s.l. with five distinct clades. These clades correspond to Bolboschoenus, Schoenoplectus s.str., and Schoenoplectiella, Scirpus s.str., and Trichophorum, respectively. These five genera were delimited in terms of the morphology of tuber, bract, and inflorescence. By virtue of our findings, we suggest that the 17 taxa of Korean Scirpus s.l. should be placed into five genera as follows: Bolboschoenus (two species), Schoenoplectus (three species), Schoenoplectiella (six species), Scirpus s.str. (five species), and Trichophorum (one species).     

Taxonomic revision of the tribe Zoraidini (Hemiptera: Fulgoromorpha: Derbidae) from Korea

The Korean planthopper tribe Zoraidini is revised taxonomically. Five genera are recognized in the Korean fauna: Diostrombus Uhler, 1896, Losbanosia Muir, 1917, Pamendanga Distant, 1906, Shirakiana Metcalf, 1945, Zoraida Kirkaldy, 1900. Among them, the genus Shirakiana is recorded for the first time in Korea. Nine species, four of which are recognized new to Korea: S. infumata (Matsumura), Z. koannania Matsumura, Z. hubeiensis Chou et Huang, and Z. kuwayamae (Matsumura). Previous record of one species, Z. pterophoroides (Westwood, 1851), is removed from the list of Korean fauna because it was erroneously reported, based on a misidentification. All species are described and illustrated, and identification keys to genera and species are provided.     

东北阔叶红松林地表葬甲群落多样性及其空间变异特征

为了探明我国东北阔叶红松(Pinus koraiensis)林地表葬甲多样性及其空间变异特征，在胜山、丰林、凉水和长白山的4个大型森林动态监测样地内，通过布置900个陷阱调查了36 hm²范围内的地表葬甲群落基本特征。基于群落物种数量、个体数量和总体长，分析了地表葬甲多样性及其空间变异性。结果表明：(1)东北阔叶红松林区域物种库包含12个葬甲物种，密度为0.018只/m²,总平均体长达0.32 mm/m²。北方花葬甲(Nicrophorus tenuipes)是东北阔叶红松林内的广布物种，黑葬甲(Nicrophorus concolor)等五个物种具有明显的生境偏好，仅存在于单个局域物种库内。(2)物种数量、个体数量和总体长在胜山、丰林、凉水和长白山样地存在明显差异，其中纬度较低的局域物种库(凉水、长白山)可维持更高的物种多样性，纬度最高的局域物种库(胜山)维持较低的物种多样性。(3)地表葬甲群落在胜山和丰林样地存在显著的空间自相关性，在凉水和长白山样地则不显著。(4)葬甲群落具有较明显的空间异质性，长白山样地的空间变异...     

A Taxonomic Revision of Illiberis Walker (Lepidoptera: Zygaenidae: Procridinae) in Korea

The Korean species of Illiberis Walker are revised. A total of 10 species are recognized, including four species new to Korea: I. rotundata Jordan, I. psychina (OberthÜr), I. consimilis Leech, and I. hyalina (Staudinger). The identities of I. cybele Leech and I. assimilis Jordan, the two ambiguously defined Korean species, are reconfirmed with the examination of type specimens and additional materials. Photos of the adults and type materials are provided, and male and female genitalia of each species are illustrated. Biology and distribution for each species are briefly discussed with the larval host records from Korea.     

A new species of Clytus Laicharting (Coleoptera: Cerambycidae) from Korea with a key to Korean species

A new species of Clytus Laicharting (Coleoptera: Cerambycidae) is described from Korea. Illustrations of diagnostic characteristics of the new species including male genitalia are provided with a key to Korean Clytus species.     

Taxonomic review of Mycetophagidae Leach (Coleoptera: Tenebrionoidea) in Korea with their host fungi

A taxonomic review of the fungivorous Korean Mycetophagidae is presented. Nine species in four genera belonging to two tribes are presented in Korea. Four species, Eulagius ussuriensis Nikitsky, Litargus japonicus Reitter, Mycetophagus irroratus (Reitter), and Mycetophagus pustulosus (Reitter), are newly recorded in Korea. Most species are associated with the fruiting bodies of higher fungi, fungus‐grown bark, and mold contaminated materials. In addition, we provide information on host‐fungi records of Korean mycetophagid species.