黄花石蒜中的黄酮类成分

通过对黄花石蒜（Lycoris aurea）乙醇提取物的乙酸乙酯溶解部分进行分离纯化,得到7个化合物.经物理方法和波谱分析,分别鉴定为（2S）-4＇-羟基-7-甲氧基黄烷（1）、（2S）-3＇,7-二羟基-4＇-甲氧基黄烷（2）、（2S）-4＇,7-二羟基黄烷（3）、（2S）-4＇,7-二羟基-8-甲基黄烷（4）、（2S）-4＇,7-二羟基-3＇-甲氧基-8-甲基黄烷（5）、（2S）-4＇,5,7-三羟基-8-甲基黄烷酮（6）、2,4＇-二羟基-4-甲氧基二氢查尔酮（7）.所有化合物均为首次从该属植物中分离得到,另外,本文首次归属了化合物5、6、7的13C NMR信号.     

肾茶化学成分的研究

从肾茶中分离得到11个已知化合物,通过NMR及MS数据分析,分别鉴定为：5-羟基-6,7,4’-三甲氧基黄酮（1）,5-羟基-6,7,3’,4＇-四甲氧基黄烷酮（2）,orthosipholF（3）,siphonolB（4）,白桦酯酸（5）,2α-羟基齐墩果酸（6）,2α,3α-二羟基-12-烯-28-齐墩果酸（7）,委陵菜酸（8）,蔷薇酸（9）,熊果酸（10）和胡萝卜甙（11）。其中,花合物2-9为首次从该植物中分离得到。     

镰形棘豆化学成分的研究

采用柱层析(CC)、短柱减压层析、重结晶等分离手段,从镰形棘豆乙醇提取液中分得7个已知化合物,经波谱分析鉴定为N-苯甲酰基-2-苯基乙胺(1),N-肉桂酰基-2-苯基-2-乙胺(2),N-苯甲酰基-2-羟基-2-苯基乙胺(3),2′-羟基-4′-甲氧基查儿酮(4),7-甲氧基黄烷酮(5)、5-羟基-7-甲氧基黄烷酮(6),β-谷甾醇(7).化合物1～3、5、6均为首次从该植物中分到.药理实验表明化合物4,5对人胃癌细胞株SGC有较强的细胞毒活性,IC50分别为3.61和6.11 μg/mL.     

飞机草地上部分化学成分及其抑菌活性研究

为探究飞机草地上部分化学成分及其抑菌作用。采用正相硅胶、Sephadex LH-20等色谱方法,从飞机草乙酸乙酯提取物中得到10个化合物,根据1H NMR、13 C NMR、MS波谱数据,分别鉴定为:5,6,7,4′-四甲氧基黄烷酮(1)、圣草素-7,4′-二甲醚(2)、5,7-二羟基-6,4′-二甲氧基黄酮(3)、二氢山柰素(4)、异野樱素(5)、4′-羟基-5,6,7-三甲氧基黄烷酮(6)、5,6,7,3′,4′-五甲氧基黄烷酮(7)、3′-羟基-5,7,8,4′-四甲氧基黄烷酮(8)、香叶木素(9)、山柰酚(10),其中,化合物2和8均首次从飞机草中分离得到。采用生长速率法测定了飞机草乙醇提取物、不同萃取物、乙酸乙酯萃取物柱层析不同馏分对4种核桃病原真菌的抑制活性,确定其活性成分集中于馏分2(石油醚∶丙酮=5∶1),选择该部分所得化合物4、5、7对4种病原菌进行毒力测定,结果表明,化合物5对拟茎点霉菌丝生长抑制作用最强,EC 50为0.1062 mg/mL。研究结果为利用飞机草开发植物源农药提供理论依据。     

凹叶瑞香的化学成分研究(英文)

从凹叶瑞香的乙酸乙酯部位分离得到14个化合物,经理化性质及波谱数据分析分别鉴定为瑞香黄烷A(1),瑞香黄烷B(2),瑞香黄烷C(3),瑞香黄烷E(4),瑞香黄烷F(5),芫花素(6),芫根苷(7),4,′5-二羟基-3,′7-二甲氧基黄酮(8),瑞香新素(9),5′′-去甲氧基瑞香新素(10),左旋松脂酚(11),瑞香醇酮(12),紫丁香苷(13)和丁香醛(14)。化合物1～14均为首次从该植物中分离得到。     

樱草杜鹃中的黄酮类化合物

从樱草杜鹃（Rhododendron primulaeflorum）地上部分的乙醇提取物中分离得到10个黄酮类化合物,经理化性质及波谱分析,鉴定为杜鹃素（1）、4’,5,7-三羟基-8-甲基黄烷酮（2）、二氢山柰酚（3）、异鼠李素（4）、槲皮素（5）、陆地棉苷（6）、瑞诺苷（7）、（2R,3R）－花旗松素-3—O-α-L-吡喃阿拉伯糖苷（8）、（2R,3S）-花旗松素-3—O-α-L-吡喃阿拉伯糖苷（9）、（2S,3R）－花旗松素－3－O－α－L－吡喃阿拉伯糖苷（10）。所有化合物均为首次从该植物中分得。     

白木香内生真菌Botryosphae riarhodina A13固体发酵产物的分离鉴定北大核心CSCD

运用硅胶柱、反相硅胶柱、凝胶柱、HPLC和薄层制备等色谱技术及重结晶从白木香内生真菌Botryosphaeria rhodina A13的固体发酵产物中分离纯化了10个化学成分,通过化合物波谱数据及理化性质鉴定它们的结构分别为豆甾-4-烯-3-酮（1）、豆甾-5-烯-3β-醇-7-酮（2）、豆甾-4-烯-3,6-二酮（3）、（22E,24S）-5α,6α-环氧基-24-甲基胆甾-8（14）,22-二烯-3β,7α-二醇（4）、5,4＇-二羟-7-甲氧基黄酮（5）、香草酸（6）、对甲氧基苯甲酸（7）、尿嘧啶（8）、2-甲氧基对苯二酚（9）、2-甲基-3,5-羟基色酮（10）。其中化合物1～10均为首次从该属真菌中分离得到,化合物4具有细胞毒活性。     

瑞香狼毒化学成分研究

从瑞香狼毒95%乙醇提取物中分离得到了17个化合物,根据理化性质和波谱数据鉴定为4’-甲氧基-7-羟基黄酮(1),山奈酚(2),5,4’-二羟基-7-甲氧基二氢黄酮(3),4’,5,7-三羟基黄烷酮(4),3,5,7-三羟基黄烷酮(5),山奈酚-7-O-β-D-葡萄糖苷(6),α-香树酯醇(7),(24R)-5α-豆甾-7,22(E)-二烯-3α-醇(8),狼毒色原酮(9),伞形花内酯(10),东莨菪素(11),异东莨菪素(12),狼毒素(13),瑞香内酯(14),异茴芹香豆素(15),β-谷甾醇(16),瑞香素乙(17)和β-胡萝卜甙(18)。其中化合物1-8为首次从该植物中分离得到。     

晶帽石斛的化学成分研究

该研究利用硅胶、凝胶、MCI、中压制备色谱(MPLC)和高效液相半制备色谱(semi-HPLC)等方法,对晶帽石斛(Dendrobium crystallinum)进行了化学成分研究。结果表明:提纯、分离共得到10个化合物,经波谱数据分析及与文献数据对照,分别鉴定为石斛酚(1),3,4'-二羟基-5-甲氧基联苄(2),3,4',5-三羟基-3-甲氧基联苄(3),二氢藜芦醇(4),安告佛醇(5),3',5,7-三羟基-4'-甲氧基黄烷酮(6),4',5,7-三羟基-6-甲氧基黄烷酮(7),丁香树脂醇(8),β-谷甾醇(9),β-胡萝卜苷(10)。其中,除化合物2和化合物10以外,其余8个化合物均在该种植物中首次发现。     

南海小束格海绵中的系列甾酮化合物

从我国南海小束格海绵（Clathria fasciculate）的乙醇提取物中分离获得一个甾酮混合物,经IR,NMR,GCMS分析和质谱数据库检索,鉴定出其中各成分的结构为：4-亚甲基-5α-胆甾烷（1）,胆甾-4,22-二烯-3-酮（2）,胆甾-4-烯-3-酮（3）,麦角甾-4,22-二烯-3-酮（4）,麦角甾-4,24（28）-二烯-3-酮（5）,麦角甾-4-烯-3-酮（6）,24-乙基-3α,5α-环甾-22-烯-6-酮（7）,豆甾-7,22-二烯-3-酮（8）和豆甾-4-烯-3-酮（9）。其中化合物3是主要成分,占总甾酮的61％。     

Neolignan glycosides from Symplocos caudata

A phytochemical investigation of the roots of Symplocos caudata Wall (Symplocaceae) resulted in isolation and characterization of four optical isomers of a neolignan glycoside (1-4), a lignan lactone glycoside (5), a phenylpropanoid glycoside (6), as well as two known compounds (7, 8). Their structures were elucidated as (7S,8S)-threo-7,9,9'-trihydroxy-3,3'-dimethoxy-8-O-4'-neolignan-4-O-beta-d-glucopyranoside (1), (7R,8R)-threo-7,9,9'-trihydroxy-3,3'-dimethoxy-8-O-4'-neolignan-4-O-beta-d-glucopyranoside (2), (7R,8S)-erythro-7,9,9'-trihydroxy-3,3'-dimethoxy-8-O-4'-neolignan-4-O-beta-d-glucopyranoside (3), (7S,8R)-erythro-7,9,9'-trihydroxy-3,3'-dimethoxy-8-O-4'-neolignan-4-O-beta-d-glucopyranoside (4), 8R,8'R-matairesinol-4-O-beta-d-xylopyranosyl-(1-->2)-O-beta-d-glucopyranoside (5), 1-O-[beta-d-xylopyranosyl-(1-->6)-O-beta-d-glucopyranosyl]-2,6-dimethoxy-4-propenyl-phenol (6), matairesinoside (7), and (R)-1-O-(beta-d-glucopyranosyl)-2-[2-methoxy-4-(omega-hydroxypropyl)-phenoxyl]-propan-3-ol (8) on the basis of spectroscopic data (1D and 2D NMR, MS and CD) and chemical evidence.     

Anti-proliferative and antioxidant constituents from Tecoma stans

Phytochemical investigation of Tecoma stans Juss. fruits and flowers resulted in the isolation of a new phenylethanoid, 2-(3,4-dihydroxyphenyl)ethyl-2-O-[6-deoxy-alpha-L-mannopyranosyl-4-(3,4-dihydroxyphenyl)-2-propenoate]-beta-D-glucopyranoside (3), and a novel monoterpene alkaloid, 5-hydroxy-skytanthine hydrochloride (8), along with eleven known compounds; 4-O-E-caffeoyl-alpha-L-rhamnopyranosyl-(1' --> 3)-alpha/beta-D-glucopyranose (1), E/Z-acetoside (2), isoacetoside (4), rutin (5), luteolin 7-O-beta-D-neohespridoside (6), luteolin 7-O-beta-D-glucopyranoside (7) and sucrose (9) were isolated from the fruits, while luteolin 7-O-beta-D-glucuronopyranoside (10), diosmetin 7-O-beta-D-glucuronopyranoside (11), diosmetin 7-O-beta-D-glucopyranoside (12), diosmetin 7-O-beta-D-glucuronopyranoside methyl ester (13) and acetoside (2) were isolated from the flowers. Their chemical structures have been determined on the basis of chemical and spectroscopic evidences. Biological investigations of a T. stans fruits extract and compounds 1, 2, 4, and 8 indicated that the extract, 1, 2, and 4 possessed a strong scavenging activity to DPPH, peroxyl and hydroxyl radicals. Unlike 4, which potentially induced NO generation in bacterial lipopolysaccharide-stimulated raw murine macrophage (RAW 264.7), the extract, 1, 2, and 8 significantly inhibited the NO generation. The extract, 2 and 4 exhibited a cytotoxic effect on human hepatocarcinoma cells (Hep-G2), while the extract, 2 and 8 were potent growth inhibitors of human breast carcinoma cells (MCF-7). 1 and 2 were remarkable growth inducers of human lymphoblastic leukemia cells (1301), whereas the extract, 2, and 8 stimulated the macrophage proliferation rate. Taken together, the novel compound 8 is effective as anti-proliferative agent against MCF-7 cells and as NO inhibitor, whereas 2 exhibited multi-functional properties as antioxidant and anti-proliferative agent against both solid tumor cell lines Hep-G2 and MCF-7 cells.     

Kinetics of nitroxyl radical reactions. A pulse-radiolysis conductivity study.

Absolute rate-constants for the reaction of the nitroxyl free radicals TAN and TMPN with radiation-chemically-formed radicals and ions have been determined. k(TAN + X) (in M(-1) sec(-1)=4-0 X 10(9) (for X = OH-), 2-9 X 10(10) (eaq-), 8-0 X 10(9) (H-), 7-2 X 10(8) (-CH2OH), 4-0 X 10(8) (CH3CHOH), 4-3 X 10(8) ((CH3)2COH) 2-8 X 10(8) (-CH2(CH3)2COH), 5-9 X 10(7) (glucose radical), 4-0 X 10(8) (c-C5H9-), and k(TMPN + X)=3-4 X 10(9) (OH-), 7-8 X 10(9) (eq-), 4-9 X 10(9) (H-), 4-4 X 10(8) (-CH2OH), 4-9 X 10(8) (CH3CHOH), 3-6 X 10(8) ((CH3)2COH), 1-5 X 10(8) (-CH2(CH3)2COH), 4-9 X 10(7) (glucose radical), 4-3 X 10(8) (c-C5H9-). Direct measurements by means of a pulse-radiolysis conductivity technique were based on the formation and destruction of charged species in these reactions within certain pH ranges. It is indicated that the radiosensitizing nitroxyls undergo both redox and addition reactions.     

Inhibitory effect on NO production of phenolic compounds from Myristica fragrans

Three new phenolics: ((7S)-8'-(benzo[3',4']dioxol-1'-yl)-7-hydroxypropyl)benzene-2,4-diol (1), ((7S)-8'-(4'-hydroxy-3'-methoxyphenyl)-7-hydroxypropyl)benzene-2,4-diol (2) and ((8R,8'S)-7-(4-hydroxy-3-methoxyphenyl)-8'-methylbutan-8-yl)-3'-methoxybenzene-4',5'-diol (3), along with four known compounds (4-7) were isolated from the seeds of Myristica fragrans. Their chemical structures were established mainly by 1D and 2D NMR techniques and mass spectrometry. Their anti-inflammatory activity was evaluated against LPS-induced NO production in macrophage RAW264.7 cells.     

Structure activity relationship studies on C2 side chain substituted 1,1-bis(4-methoxyphenyl)-2-phenylalkenes and 1,1,2-tris(4-methoxyphenyl)alkenes

1,1-bis(4-Methoxyphenyl)-2-phenylalkenes (1a-9a) and 1,1,2-tris(4-methoxyphenyl)alkenes (1b-9b) with various C2-substituents (H (1a, 1b), methyl (2a, 2b), ethyl (3a, 3b), propyl (4a, 4b), butyl (5a, 5b), 2-cyanoethyl (6a, 6b), 3-cyanopropyl (7a, 7b), 3-aminopropyl (8a, 8b), 3-carboxypropyl (9a, 9b)) were tested for cytotoxic effects on hormone dependent MCF-7 cells. The effects were correlated with agonistic and antagonist properties determined on the MCF-7-2a cell line stably transfected with the plasmid ERE(wtc)luc. We demonstrated that the antiproliferative effects did not result from an interaction with the estrogen receptor (ER). The most cytotoxic compounds 5,5-bis(4-methoxyphenyl)-4-phenylpent-4-enylamine (8a) and 4,5,5-tris(4-methoxyphenyl)pent-4-enyl (8b) showed cytocidal effects without having significant agonistic and antagonistic properties.     

连钱草化学成分研究

从连钱草(Glechoma longituba)分离并鉴定了10个化合物,分别为β-谷甾醇(1)、齐敦果酸(2)、熊果酸(3)、2α,3α-二羟基乌苏-12-烯-28-酸(4)2、α,3β-二羟基乌苏-12-烯-28-酸(5)、胡萝卜苷(6),3,6-二甲氧基-6″,6″-二甲基苯并吡喃-(7,8,2″,3″)-黄酮(7)、芫花素(8)、芹菜素-7-O--βD-葡萄糖苷(9)、木犀草素-7-O-β-D-葡萄糖甙(10)。其中化合物2,4~8首次从该植物中分离得到。     

Constituents of Pterocarpus marsupium: an ayurvedic crude drug

Five new flavonoid C-glucosides, 6-hydroxy-2-(4-hydroxybenzyl)-benzofuran-7-C-beta-d-glucopyranoside (1), 3-(alpha-methoxy-4-hydroxybenzylidene)-6-hydroxybenzo-2(3H)-furanone-7-C-beta-d-glucopyranoside (2), 2-hydroxy-2-p-hydroxybenzyl-3(2H)-6-hydroxybenzofuranone-7-C-beta-d-glucopyranoside (4), 8-(C-beta-d-glucopyranosyl)-7,3',4'-trihydroxyflavone (5) and 1,2-bis(2,4-dihydroxy,3-C-glucopyranosyl)-ethanedione (6) and two known compounds C-beta-d-glucopyranosyl-2,6-dihydroxyl benzene (7) and sesquiterpene (8), were isolated from an aqueous extract of the heartwood of Pterocarpus marsupium. The structure has been established using spectroscopic data.     

Acylated iridoid glucosides from Veronica anagallis-aquatica

Three new (1-3) and four known iridoid glucosides (4-7) as well as a known phenylethanoid glycoside (8) were isolated from the aerial parts of Veronica anagallis-aquatica and their structures were determined as 6'-O-benzoyl-8-epiloganic acid named aquaticoside A (1), 6'-O-p-hydroxybenzoyl-8-epiloganic acid named aquaticoside B (2), 6'-O-benzoyl-gardoside named aquaticoside C (3), veronicoside (4), catalposide (5), verproside (6), verminoside (7) and martynoside (8) on the basis of 1D and 2D NMR spectral analysis.     

The Chemical Constituents of Elsholtzia densa Benth.

Ten compounds were isolated from Elsholtzia densa Benth. and their structures were identified by spectral and chemical methods as following: n-nonacosane (1), succinic acid (2), 5- ( 3 ”, 3 ”-dimethylallyl ) -8-methoxyfurocoumarin (3), 5- ( 3 ”-methylbutyl) -8- methoxyfurocoumarin (4), 5- (3”-hydroxy-3”-methylbutyl) -8-methoxyfurocoumarin (5), 3, 4-dihydroxycinnamic acid ( 6 ), 5-hydroxy-3’-methoxyflavanone-7-O-rutinoside ( 7 ), quercetin-3-O-β-D-glucoside ( 8 ), kaempferol-3-O-β-D-glucoside ( 9 ), 5-hydroxy-4’- methoxyflavone-7-O-rutinoside (10). Among them, compounds 4 and 5 are new naturally occurring furocoumarins.     

Prolyl endopeptidase inhibitors from Syzygium samarangense (Blume) Merr. & L. M. Perry

Compounds isolated from the hexane extract of the leaves of Syzygium samarangense (Blume) Merr. & L. M. Perry were tested for inhibitory activity against the following serine proteases: trypsin, thrombin and prolyl endopeptidase. The compounds were identified as an intractable mixture of alpha-carotene and beta-carotene (1), lupeol (2), betulin (3), epi-betulinic acid (4), 2',4'-dihydroxy-6'-methoxy-3'-methylchalcone (5), 2'-hydroxy-4',6'-dimethoxy-3'-methylchalcone (6), 2',4'-dihydroxy-6'-methoxy-3',5'-dimethylchalcone (7), 2',4'-dihydroxy-6'-methoxy-3'-methyldihydrochalcone (8) and 7-hydroxy-5-methoxy-6,8-dimethylflavanone (9). Hydrogenation of compounds 5, 6 and 7 yielded compound 8, 2'-hydroxy-4',6'-dimethoxy-3'-methyldihydrochalcone (10) and 2',4'-dihydroxy-6'-methoxy-3',5'-dimethyldihydrochalcone (11), respectively. The hydrogenated products of compounds 6 and 7 were also tested for enzyme inhibitory activity. In addition, beta-sitosterol (12) and beta-D-sitosterylglucoside (13) were also isolated. This is the first report of the isolation of compounds 1-6, 8 and 13 from this plant. Compounds 3-8 and 10 exhibited significant and selective inhibition against prolyl endopeptidase among three serine proteases. This is the first report of this kind of activity for all these compounds.