共查询到20条相似文献,搜索用时 31 毫秒
1.
Hypocotyl expiants from 22 cultivars ofCatharanthus roseus were cultured on various shoot-inducing media to assess their competence for adventitious shoot formation. The Murashige
and Skoog (MS) media had been supplemented with 14 μM zeatin and 2.5 μM indole-3-butyric acid (IBA), 4.5 μM BA and 0.5 μM
α-naphthaleneacetic acid (NAA), or 14 μM thidiazuron and 2.5 μM IBA. After eight weeks, the expiants from ‘Cooler Raspberry
Red’ showed the greatest frequency of adventitious shoot formation, followed by ‘Cooler Orchid’ and ‘Cooler Treated’. The
highest frequency (86.7%) for ‘Cooler Raspberry Red’ was attained on the medium enhanced with 14 μM zeatin and 2.5 μM NAA.
Excised adventitious shoots were then readily rooted on a half-strength MS basal medium. Afterward, the regenerated plantlets
were transferred to potting soil and grown to maturity in a greenhouse. 相似文献
2.
Adventitious shoot regeneration from leaf explants of southern highbush blueberry cultivars 总被引:1,自引:0,他引:1
Cheng Liu Pete Callow Lisa J. Rowland James F. Hancock Guo-qing Song 《Plant Cell, Tissue and Organ Culture》2010,103(1):137-144
Protocols were developed to optimize adventitious shoot regeneration from four southern highbush blueberry cultivars. Leaf
explants from 6 week-old shoots of the four cultivars were excised and cultured on woody plant medium each containing thidiazuron
(4.54 or 9.08 μM), zeatin (18.2 μM), or zeatin riboside (5.7 or 11.4 μM) either separately or in combination with α-naphthaleneacetic
acid at 2.69 μM. Optimum medium for shoot regeneration was genotype-dependent. Efficient regeneration was obtained at frequencies
of 88.9% for ‘Jewel’, 87.8% for ‘Emerald’, 53.3% for ‘Jubilee’ and 87.8% for ‘Biloxi’. Leaf explants of newly developed shoots
from the cultures having undergone five subcultures had higher regeneration frequencies than those having undergone two subcultures.
Regenerated shoots, 80–100% for each cultivar, rooted in 8 weeks after transplantation to soil. The regeneration systems described
have potential use in genetic transformation of southern highbush blueberry cultivars. 相似文献
3.
Summary An improved protocol for shoot regeneration from hypocotyl segments of seedlings from open-pollinated seeds of lingonberry
(Vaccinium vitis-idaea L.) cultivars, ‘Ida’, ‘Splendor’, and ‘Erntesegen’, and a native clone from Newfoundland was developed. The effect of thidiazuron
(TDZ) on adventitious bud and shoot formation from apical, central, and basal segments of the hypocotyl was tested. Highly
regenerative callus was obtained from hypocotyl segments on modified Murashige and Skoog (MMS) medium containing 5–10 μM TDZ. A maximum of 10 buds and 12 shoots per apical segment for seedlings of cultivar ‘Ida’ regenerated on MMS containing
10 μM TDZ. Callus and bud regeneration frequency, callus growth, and number of buds and shoots per regenerating explant depended
not only on the specific segment of the hypocotyl, but also on parental genotype. Inhibition of shoot elongation by TDZ was
overcome by transferring shoot cultures to a shoot proliferation medium containing 1–2 μM zeatin. The optimal concentration of sucrose for shoot elongation was 20 gl−1. Shoots were rooted ex vitro on a 2 peat: 1 perlite (v/v) medium after dipping in 0.8% indole-3-butyric acid, and rooted plants acclimatized readily under
greenhouse conditions. 相似文献
4.
J. Y. Choi H. J. Kim C. H. Lee J. M. Bae Y. S. Chung J. S. Shin N. I. Hyung 《In vitro cellular & developmental biology. Plant》2001,37(2):274-279
Summary An efficient and simple plant regeneration system via organogenesis from leaf segments of persimmon (Diospyros kaki Thunb.) cultivars ‘Fuyu’ and ‘Nishimurawase’ has been developed. The regeneration capacity was influenced by the culture
vessels, gelling agents, plant growth regulators, and light conditions. Leaf explants taken from in vitro shoots were cultured on a modified Murashige and Skoog medium (MS1/2N), for 16 wk without transfer to fresh medium. Adventious
shoots appeared after 4 and 8 wk in culture of ‘Nishimurawase’ and ‘Fuyu’ tissues, respectively. The culture of leaf explants
in Erlenmeyer flasks with medium containing 4 g l−1 agar enhanced shoot formation in comparison to media with increased agar concentrations. Optimal shoot regeneration was obtained
with 5 mg l−1 (22.8 μM) zeatin and 0.1 mg l−1 (0.05 μM) indole-3-butyric acid (IBA) for ‘Nishimurawase’, and 10 mg l−1 (45.6 μM) zeatin and 0.1 mg l−1 (0.05 μM) IBA for ‘Fuyn’. Shoot regeneration frequencies in both cultivars were 100%, and shoot numbers per explant reached up to
9.2 for ‘Nishimurawase’ and 2.2 for ‘Fuyu’. Dark incubation during the first 4–5 wk was the most effective condition to successfully
influence shoot regeneration in both cultivars. While dark incubation was essential for adventitious shoot formation by ‘Fuyu’,
it was only slightly beneficial to ‘Nishimurawase’. More than 80% of the regenerated shoots rooted within 4 wk on hormone-free
MS1/2N demium after having been dipped for 30 s in 250 mg l−1 (1.1. mM) IBA solution. 相似文献
5.
The effects of indole-butyric acid (IBA) and electro-pulses on rooting and shoot growth were studied in vitro, using olive
shoot cultures. Tested shoots were obtained from seedlings belonging to three Spanish cultivars, ‘Arbequina’, ‘Manzanilla
de Sevilla’ and ‘Gordal Sevillana’, which have easy-, medium- and difficult-to-root rooting abilities, respectively. The standard
two-step rooting method (SRM), consisting of root induction in olive rooting medium supplemented with 0, 0.1 or 1 mg/l IBA
followed by root elongation in the same rooting medium without IBA, was compared with a novel one-step method consisting of
shoot electro-pulses of 250, 1,250 or 2,500 V in a solution of IBA (0, 0.1 or 1 mg/l) and direct transferral to root elongation
medium. The rooting percentage of the seedling-derived shoots obtained with the SRM was 76% for ‘Arbequina’ and ‘Gordal Sevillana’
cultivars and 100% for ‘Manzanilla de Sevilla’ cultivar, whereas with the electro-pulse method, the rooting percentages were
68, 64 and 88%, respectively. IBA dipping without pulse produced 0% rooting in ‘Arbequina’ seedling-derived shoots. The electroporation
in IBA not only had an effect on shoot rooting but also on shoot growth and development, with longer shoots and higher axillary
shoot sprouting and growth after some of the treatments. These effects were cultivar-dependent. The electro-pulse per se could
explain some of these effects on shoot development.
I.M.G. Padilla and I. Vidoy contributed equally to this article. 相似文献
6.
Effect of 1,2-benzisoxazole-3-acetic acid on adventitious shoot regeneration and in vitro rooting in apple 总被引:3,自引:0,他引:3
The effect of 1,2-benzisoxazole-3-acetic acid (BOA), compared to 1-naphthaleneacetic acid (NAA), on adventitious shoot formation
in leaf portions and compared to indolebutyric acid (IBA), on in vitro rooting in the apple (Malus domestica Borkh) cultivars McIntosh and Gala, and one rootstock, Jork 9, was investigated. BOA at 43.0 μm or 2.7 μm at NAA in combination
with 17.8 μm benzyladenine (BA), induced the highest number of explants to produce adventitious shoots in Jork 9. In Gala,
the combination of 21.5 μm BOA with 1.0 μm thidiazuron (TDZ) or with 22.0 μm BA induced the highest regeneration percentages,
58 and 54%, respectively, giving more satisfactory results than NAA (where only 42% of leaf explants exhibited shoot formation).
In McIntosh, the highest percentage of regeneration was obtained with 1.3 μm NAA and 22.0 μm BA, while 51% was the highest
response obtained with the BOA treatment. The combination of BOA with TDZ completely inhibited regeneration activity in leaf
portions of this cultivar. The shoots of all the genotypes obtained with the most morphogenetic NAA or BOA treatments were
excised, multiplied and successfully rooted and hardened. The results demonstrate that the synthetic auxin BOA is active in
inducing shoot regeneration from leaf explants of apple and that the activity of BOA in plant regeneration is genotype dependent.
When BOA was used to induce rooting in apple microcuttings, lower rooting percentages were obtained than with IBA, showing
that the effect of BOA in inducing root formation is very low and that it cannot be used routinely to replace IBA in the in
vitro rooting of microcuttings.
Received: 18 June 1998 / Revision received: 4 January 1999 / Accepted: 29 January 1999 相似文献
7.
Samir C. Debnath 《Plant Cell, Tissue and Organ Culture》2008,93(2):231-240
Shoot proliferation and rooting of three cranberry (Vaccinium macrocarpon Ait.) cultivars Bergman, Pilgrim, and Stevens were obtained in vitro on a modified nutrient medium containing zeatin following
a one-step procedure. Bergman and Stevens differed in terms of shoot height, leaf number per shoot, rooting frequency, root
number per explant, and root length; this was manifested with various concentrations of zeatin. Shoots proliferated and roots
developed best when nodal segments were cultured in the medium supplemented with very low concentration of zeatin (2–4 μM).
Such zeatin-induced tissue culture (TC) shoots of Bergman, Pilgrim, and Stevens were rooted ex vitro and compared with those
propagated by conventional softwood cuttings (SC) for growth and morphology over four growth seasons. Significant interactions
for leaf number per upright were observed among the treatments. The cultivars differed in terms of runner number per plant,
upright length, number of leaves per upright, and shoot vigor. The propagation method had an effect on morphology of cranberry
plants. The TC plants produced more runners and uprights with more leaves per upright than the conventional cuttings. This
increase in vegetative growth of in vitro-derived plants over stem cuttings varied among genotypes. In vitro culture on zeatin-containing
nutrient medium apparently induces the juvenile branching characteristics that favored enhanced vegetative growth with more
shoots and leaf production. 相似文献
8.
Phillip J. Ainsley Graham G. Collins Margaret Sedgley 《In vitro cellular & developmental biology. Plant》2001,37(6):778-785
Summary Shoot cultures of the paper shell almond (Prunus dulcis Mill.) cultivars ‘Ne Plus Ultra’ and ‘Nonpareil’ were subcultured for 4 wk at 4°C on growth regulator-free basal medium under
low light conditions. Elongated shoots were excised and their response to a range of rooting treatments determined. Various
concentrations of indole-3-butyric acid (IBA) and α-naphthaleneacetic acid were compared over a range of incubation periods
to determine the optimum auxin for root formation. In addition, the effect of shoot base shading, phloroglucinol (PG), and
basal salt composition were examined. The treatment resulting in the best rooting of both cultivars was shoot insertion for
12 h into water-agar (0.6% w/v) with 1.0 mM IBA, followed by 2 wk in basal medium without auxin but with 100.0 μM PG. Explants were maintained under dark conditions for 3 d at the start of the treatment period, then exposed to light. Extending
the darkening period did not improve rooting ability. Whilst half-strength Murashige and Skoog basal medium was suitable for
rooting “Ne Plus Ultra’ shoots, full-strength Almehdi and Parfitt medium resulted in the best rooting of ‘Nonpareil’. Under
these conditions, 60.0% of explants developed adventitious roots. 相似文献
9.
Breeding linseed (Linum usitatissimum L.) using haploid techniques allows breeders to develop new cultivars in a shorter time period. Many research groups successfully
created new linseed genotypes through anther culture; however ovary culture has been the subject of only a few earlier studies.
In the present study, the effect of genotype and growth regulators combination on callus induction and shoots regeneration
in ovary culture of nine commercially important linseed cultivars was investigated. Ovaries were cultured on modified MS medium
supplemented with three different combinations of plant growth regulators. Variable callogenic responses were expressed by
all of the genotypes tested on different induction media. The results suggested that specific combination of growth regulators
for callus induction must be designed for each genotype. Shoot regeneration from ovary derived callus is a critical phase
of the whole gynogenetic process. Differences in adventitious shoot formation frequency among genotypes were demonstrated
and four responsive genotypes have been selected. Ovary derived callus from cultivar ‘Mikael’ manifested the highest adventitious
shoot formation frequency with a high number of shoots per explant. The optimum ratio of growth regulators for shoot regeneration
was shown to depend on the genotype. Cultivars ‘Linola’, ‘Mikael’ and ‘Szaphir’ showed the highest shoot regeneration frequency
when callus had originated on induction medium supplemented with 2 mg L−1 BAP and 2 mg L−1 NAA, while combination of 1 mg L−1 BAP and 2 mg L−1 IAA promoted shoot formation in ovary-derived callus of ‘Barbara’. The highest rate of shoots per explant has been obtained
in second subculture. 相似文献
10.
High Frequency of Shoot Regeneration from Hypocotyls and Stem Segments of Antirrhinum majus (Snapdragon) 总被引:1,自引:0,他引:1
A broadly applicable direct shoot regeneration method from hypocotyls and stem explants has been developed for six cultivars
of Antirrhinum majus L. In order to establish a stable and high frequency of shoot regeneration system, leaves, hypocotyls and stem explants of
six cultivars were tested with 72 combinations of auxin (naphthaleneacetic acid (NAA) or 3-indoleacetic acid (IAA)) and cytokinin
(6-benzylaminopurine (BA) or zeatin (Z)). A few adventitious shoots were directly regenerated from hypocotyl segments of cv.
Orchid on MS medium with NAA + BA, IAA + BA, NAA + Z and IAA + Z. High frequency of direct shoot regeneration was obtained
from hypocotyl segments on MS medium with 0.05, 0.1 or 0.25 mg l−1 NAA + 2 mg l−1 Z and 0.5 mg l−1 IAA + 2 mg l−1 Z. Finally, stable and high frequency (92–100%) of shoot regeneration with more than 10 adventitious shoots per explant was
achieved from the hypocotyls and stem explants of all six cultivars on MS medium with 0.25 mg l−1 NAA + 2 mg l−1 Z. The shoots emerged directly from the hypocotyls and stem segments 4 weeks after culture initiation. 相似文献
11.
S. C. Debnath 《In vitro cellular & developmental biology. Plant》2009,45(2):122-128
An efficient system to regenerate shoots on excised leaves of greenhouse-grown wild lowbush blueberry (Vaccinium angustifolium Ait.) was developed in vitro. The effect of thidiazuron (TDZ) on adventitious bud and shoot formation from apical, medial, and basal segments of the leaves
was tested. Leaf cultures produced multiple buds and shoots with or without an intermediary callus phase on 2.3–4.5 μM TDZ
within 6 wk of culture initiation. The greatest shoot regeneration came from young expanding basal leaf segments positioned
with the adaxial side touching the culture medium and maintained for 2 wk in darkness. Callus development and shoot regeneration
depended not only on the polarity of the explants but also on the genotype of the clone that supplied the explant material.
TDZ-initiated cultures were transferred to medium containing 2.3–4.6 μM zeatin and produced usable shoots after one additional
subculture. Elongated shoots were dipped in 39.4 mM indole-3-butyric acid powder and planted on a peat:perlite soilless medium
at a ratio of 3:2 (v/v), which yielded an 80–90% rooting efficiency. The plantlets were acclimatized and eventually established in the greenhouse
with 75–85% survival. 相似文献
12.
Summary Carbohydrate type and concentration and their interactive effects on in vitro shoot proliferation of three lingonberry (Vaccinium vitis-idaea ssp. vitis-idaea L.) cultivars (‘Regal’, ‘Splendor’, and ‘Erntedank’) and two V. vitis-idaea ssp. minus (Lodd) clones (‘NL1’ and ‘NL2’) were studied. Nodal explants were grown in vitro on medium with 2 μM zeatin and either glucose, sorbitol, or sucrose at a concentration of 0, 10, 20, or 30 gl−1. The interactive effects of carbohydrate type and concentration and genotype were important for shoot proliferation. The
best response was afforded by sucrose at 20 gl−1 both in terms of explant response and shoot developing potential, although glucose supported shoot growth equally well, and
in ‘NL1’ at 10 gl−1 it resulted in better in vitro growth than sucrose. Carbohydrate concentration had little effect on shoot vigor. The genotypes differed in terms of shoots
per explant, length, and vigor, leaves per shoot, and callus formation at the base of explants; this was manifested with various
types and concentrations of carbohydrate. Changing the positioning of explants on the medium from vertically upright to horizontal
increased the shoot and callus size, but decreased shoot height and leaves per shoot. Proliferated shoots were rooted on a
peat:perlite (1∶1, v/v) medium and the plantlets were acclimatized and eventually established in the greenhouse. 相似文献
13.
The effects of post harvest application of ethylene, abscisic acid (ABA), indole-3-butyric acid (IBA) treatments or dark storage
on root induction and continued growth of regenerated roots in Pelargonium cuttings were investigated using hydroponics in the greenhouse. Ethylene markedly increased rooting percentage in ‘Greco’
and ‘Surfing’, reduced the number of roots per cutting in ‘Surfing’ and had no effect on the total root lengths in the two
cultivars. Ethylene treatment reduced fresh root mass in ‘Surfing’, increased dry root mass and reduced root water content
in both cultivars. ABA (50 μM) enhanced rooting percentage in ‘Greco’, reduced the number of roots per cutting, reduced total
root lengths and fresh root mass in both cultivars. ABA increased dry root mass and reduced root water content in ‘Surfing’
but this effect was not apparent in ‘Greco’. Storing cuttings in the dark for 4 days had no effect on rooting percentage and
number of roots per cutting in ‘Greco’ and ‘Surfing’. However, dark storage reduced total root lengths in ‘Surfing’ and reduced
fresh root mass in ‘Greco’. Dark storage had no effect on dry root mass and water content in both cultivars. Applying 4 μl l−1 IBA in the rooting solution induced maximum (100%) root induction in ‘Surfing’. However, IBA reduced the number of roots
per cutting in ‘Greco’, reduced total root lengths and fresh root mass in the two cultivars. IBA treatment profoundly increased
and reduced dry root mass and root water content, respectively, in ‘Greco’ and ‘Surfing’. The enhanced root induction observed
after IBA and ABA applications could be ascribed to their influence on ethylene biosynthesis, since ethylene treatment increased
rooting percentage in both cultivars. However, high ABA (100 μM) and IBA (12 μl l−1) levels or dark storage reduced the ability of induced roots to continue growth. We attribute our results to plant stress-response
mechanism and ethylene appears to play an important role in the process of root initiation and root growth in Pelargonium cuttings. 相似文献
14.
Richard L. Bell Ralph Scorza Delores Lomberk 《Plant Cell, Tissue and Organ Culture》2012,108(2):229-236
Adventitious shoot regeneration of twenty-four pear genotypes was compared in a common in vitro shoot induction and development
protocol. This study also compared cultures newly established from scionwood with cultures that been in long-term cold storage.
In vitro cultures of 13 Pyrus genotypes and budwood from 23 Pyrus genotypes were obtained from the National Clonal Germplasm Repository (NCGR) in Corvallis, Oregon. With the exception of
one genotype of P. elaeagrifolia Pall., and ‘Ya Li’ (P. pyrifolia var. sinensis Teng & Tanabe), all were P. communis L. cultivars. The basal shoot induction media consisted of Chevreau and Leblay (CL) basal nutrients, vitamins, and organics
(Chevreau and Leblay in Acta Hortic 336: 263–268, 1993). The analysis of variance indicated that differences among genotypes were highly significant and the main effect of culture
origin was non-significant. However, there was a significant interaction between genotype and culture origin, with percentage
regeneration of ‘Abate Fetel’ from new budwood significantly greater than that from long-term in vitro cultures, while ‘Jesinji
Vodenac’ cultures derived from the old NCGR cultures regenerated significantly more adventitious shoots. The ranges of mean
regeneration frequency were similar for both in vitro (0–87.7%) and scionwood-derived cultures (0–70.7%). Maximum regeneration
was observed for ‘Conference’, followed by ‘Magness’, ‘Dr. Jules Guyot’, and Packham’s Triumph’. The range of number of adventitious
shoots was relatively narrow, with the minimum of 1.0 for seven genotypes to 2.2 for ‘Conference’. 相似文献
15.
Samir C. Debnath 《Plant Growth Regulation》2007,51(3):245-253
The effects of four indole-3-butyric acid (IBA) concentrations and two propagation methods were studied in a lowbush blueberry
(Vaccinium angustifolium Ait.) clone collected from natural stands in Newfoundland and Labrador, Canada. Lowbush blueberry cultures were established
in vitro from nodal explants on a modified cranberry (V. macrocarpon Ait.) tissue culture medium containing zeatin (2 μM). Blueberry plants propagated by in vitro shoot proliferation (TC) and
by conventional softwood cuttings (SC) were evaluated for growth and morphology. Significant interactions for morphological
characteristics were observed among the treatments. The IBA concentration had an effect on morphology of propagated plants,
increasing the concentration of IBA increased stem length and leaves per stem across propagation methods. Stems per plant
increased with IBA concentration up to 20 μM in SC plants, but not in TC plants. Plant vigor was affected by neither IBA concentration
nor propagation method. The TC plants produced longer and more stems with more leaves per stem than the conventional cuttings.
In vitro culture on zeatin-containing nutrient medium apparently induces the juvenile branching characteristics that favored
enhanced vegetative growth with more stems and leaf production. It is suggested that IBA may serve as a physiologically active
form of auxin in contributing to increased stem and leaf production in lowbush blueberry SC plants but not in TC plants. 相似文献
16.
Wenhao Dai Yuanjie Su Cielo Castillo Olivier Beslot 《Plant Cell, Tissue and Organ Culture》2011,104(2):257-262
Shoots were regenerated from in vitro leaf tissues of two genotypes of Viburnum dentatum, a popular shrub species for landscape use. Adventitious shoots were induced when leaf tissues were cultured on woody plant
medium (WPM) supplemented with either benzyladenine (BA) or thidiazuron (TDZ). Effects of cytokinin concentration, indole-3-butyric
acid (IBA), and dark treatment on shoot regeneration were investigated. Dark treatment for the first 4 weeks of leaf explants
cultured in the regeneration medium significantly increased the frequency of regeneration. The highest frequency of shoot
regeneration (70%) for ‘Synnesvedt’ was obtained when leaf tissues were cultured in the medium with 40 μM BA or 8 μM TDZ with
4 weeks dark treatment. The highest frequency of shoot regeneration (90%) for ‘MN34’ was found in the 4 μM TDZ medium with
4 weeks dark treatment. Addition of IBA significantly enhanced shoot regeneration. Ethyl methanesulfonate (EMS) treatment
inhibited callus proliferation, particularly in the early stage of callus recovery; however, no significant difference in
shoot regeneration among different treatments was observed, indicating that the inhibitory effect of EMS was minimal after
calluses re-acquired their capacity to grow and regenerate in the regular medium. Regenerated shoots (>1.5 cm) were rooted
in the half-strength MS medium containing 5-10 μM IBA or naphthalene acetic acid (NAA). Rooted plants were transferred to
the potting medium and grown in the greenhouse. 相似文献
17.
Andrzej Pacholczak Wiesław Szydło Aleksandra Łukaszewska 《Acta Physiologiae Plantarum》2006,28(6):567-575
In order to improve vegetative propagation of Berberis thunbergii ‘Red Rocket’, stock plants were subjected to shading and IBA treatment, as well as to the application of two commercially
available rooting powders. Spraying stock plants with IBA 24 hours prior to cutting harvest gave the effect comparable to
the use of rooting powders. Reduction of light intensity by 50% and 96% of ambient prior to harvest of cuttings improved rooting.
Positive effect of shading results in changes in shoot anatomy, i.e. a weaker sclerenchyma development. Enhanced rooting in cuttings from shoots grown under reduced light intensity was accompanied
by a decrease in the concentration of soluble proteins and by an increase in total chlorophyll content while the concentration
of total soluble sugars remained unaffected. 相似文献
18.
Yeh-Jin Ahn Louisa Vang Thomas A. McKeon Grace Q. Chen 《In vitro cellular & developmental biology. Plant》2007,43(1):9-15
An efficient plant regeneration protocol was established for castor (Ricinus communis L.). Hypocotyl tissue from zygotic embryo axis produced adventitious shoots when treated with either thidiazuron (TDZ, 1 μM)
or 6-benzylaminopurine (BA, 20 μM). TDZ resulted in more than a threefold higher rate of shoot induction (a maximum of 24.2
shoots per explant) than BA (6.8 shoots). Our results also showed that the pretreatment of explants in the dark increased
the number of shoots regenerated per explant by 82% and 36% with TDZ and BA, respectively. The elongation of hypocotyl tissue
in the dark appears to be the primary cause of the increase. Comparable rates of rooting were achieved on the media supplemented
with either indole-3-butyric acid (IBA, 84.3%) or 1-naphthaleneacetic acid (NAA, 87.4%) at 5 μM. However, IBA was more efficient
in promoting root and shoot development, resulting in a higher rate of establishment (93.5%) in the soil, compared to the
rate with NAA (39.5%). Histological analysis showed the adventitious induction of the shoot buds originated from the cortex
of the hypocotyl tissue. 相似文献
19.
Luping Qu Jianjn Chen Richard J. Henny Yingfeng Huang Russell D. Caldwell Cynthia A. Robinson 《In vitro cellular & developmental biology. Plant》2002,38(3):268-271
Summary Regeneration of adventitious shoots of pothos (Epipremnum aureum Linden and Andre) ‘Jade’ was obtained using leaf and petiole explants preprated from shoot tips of 3-yr-old greenhouse-grown
plants. Explants were cultured on Murashige and Skoog (MS) basal medium supplemented with thidiazuron (TDZ), 6-(4-hydroxy-3-methy-trans-2-butenyl-amino)purine
(zeatin) or N-isopentenylaminopurine (2iP) individually with α-naphthaleneacetic acid (NAA) in 18 combinations. Callus was initiated from
cut surface and along the midrib or major vein of leaf sections. Shoot regeneration from leaf and petoole explants occurred
in 30d on medium containing 1, 5 or 10μM TDZ with 0.5 or 1.0μM NAA except petioles on medium with 10 μM TDZ and 1.0 μM NAA where regeneration failed. More time (50d) was needed for shoot regeneration when explants were cultured on medium containing
either 2iP or zeatin with NAA. Regeneration frequencies were up to 20% and 50% for leaf and petiole explants, respectively.
Shoot numbers per responding explant attained 30 for leaf and petiole explants on medium containing TDZ but only one to four
on medium containing either 2iP or zeatin. These results indicate that TDZ is a more effective cytokinin for in vitro regeneration of pothos than either zeatin or 2iP.. Shoots elongated readily and rooted well on MS basal medium, without plant
growth regulators. Plantlets acclimatized rapidly and grew vigorously in the greenhouse after transfer to pots containing
a commerecial potting medium. 相似文献
20.
Phillip J. Ainsley Graham G. Collins Margaret Sedgley 《In vitro cellular & developmental biology. Plant》2000,36(6):470-474
Summary A method has been developed to facilitate shoot formation from leaf explants of almond. Leaves were dissected from micropropagated
shoot cultures of the commercial cultivars Nonpareil and Ne Plus Ultra, and sections incubated on Almehdi and Parfitt's (1986)
basal medium (AP) with varied plant growth-regulator conditions. Three auxins, 2,4-dichlorophenoxyacetic acid (2,4-D), α-naphthaleneacetic
acid (NAA), and indole-3-butyric acid (IBA), in combination with two cytokinins, benzylaminopurine (BA) and thidiazuron (TDZ),
were tested at various concentrations along with casein hydrolysate (CH) to determine, the conditions most conducive to adventitious
shoot regeneration. Response to the tested plant growth-regulator conditions varied with genotype. Of the three auxins tested,
NAA and IBA induced adventitious shoots from Ne Plus Ultra explants, but only IBA was effective for Nonpareil. For the cytokinins,
shoot development from Ne Plus Ultra occurred in the presence of either BA or TDZ, whereas for Nonpareil only TDZ was effective
unless CH was incorporated in the basal medium. The inclusion of CH (0.1% w/v) improved callus morphology, and increased regeneration
frequencies for both cultivars. Maximum regeneration frequencies for Ne Plus Ultra (44.4%) and Nonpareil (5.5%) were achieved
on AP basal salts supplemented with CH, IBA (9.8 μM), and TDZ at 22.7 and 6.8 μM, respectively. 相似文献