中国球虎天牛属Calloides一新种(鞘翅目:天牛科:天牛亚科)

本文报道球虎天牛属Calloides1新种——云南球虎天牛Calloidesyunnanensissp.nov.,模式标本保存在西南大学昆虫标本馆。该新种与黄带刺虎天牛C.magnificus(Pic)近似,主要区别特征是第3、4节触角之比为3∶2而非1∶1;鞘翅黄色,具3对黑斑而非黑色具3对黄带。正模:♀,云南省威信县,1980-Ⅵ-24,陶正武采。     

云南天牛一新种淑氏并脊天牛描述 (鞘翅目,天牛科,沟胫天牛亚科,楔天牛族)

描述了中国云南省的1天牛新种淑氏并脊天牛Glenea shuteae sp.nov.。对与之最相似的变色并脊天牛G.decolorata Heller,1926进行了补充描述。提供了2个相似种的整体照片及外生殖器图片。模式标本保存在中国科学院动物研究所和英国伦敦自然历史博物馆。淑氏并脊天牛,新种Glenea shuteae sp.nov.(图1～6,16)新种淑氏并脊天牛与变色并脊天牛斑纹相似,但可以通过以下特征区分:雄虫3对足的爪均有且仅有外侧齿的外侧基部具小而尖锐的附齿(图16),而不是单齿式(图17);前胸背板具清晰的纵条纹;雄性外生殖器的阳基侧突正常(图4),没有不对称;腹部第8背板末端更加尖突(图5)。正模♂,云南西双版纳小勐养,海拔850m,1957-06-14,臧令超采(IZAS)。副模:2♂♂,西双版纳勐阿,海拔1050～1080m,1958-06-01,蒲富基采(IZAS);1♀,西双版纳,勐阿,1958-10-01(IZAS);1♂,西双版纳勐遮,海拔870m,1958-07-06,王书永采(IZAS);1♂,西双版纳,景洪市西北37km,纳板河流域国家级自然保护区,勐海县过门山(22°1...     

湖南星天牛属一新种(鞘翅目:天牛科, 沟胫天牛亚科)

蒋氏星天牛Anoplopphora(s.s.)chiangi Hua et Zhang新种(图1) 雌虫 体漆黑色有光泽,光裸。触角第2节,第3—11节基部及端部具蓝白色毛环。小盾片后缘被少量淡蓝色细绒毛。胫节中部背面及两侧,跗节(除第4节外)背面被淡蓝白色绒毛。头部刻点微小稀疏,眼后部分刻点较粗;额近方形,高略胜于宽,中沟细,伸达后头后缘;头顶深陷;复眼下叶近椭圆形,约为其下颊长的1.5倍。触角基瘤突出;触角1.5倍于长,柄节稍短于第4节,为第3节长的3/4。前胸背板横阔,前、后横沟明显,侧刺突钝,背面中央两侧各具一小突起,中央后方具一较宽大的突起,中央后部两侧散布少数刻     

海南岛星天牛属一新种：鞘翅目：天牛科

尖峰星天牛Anoplophora(s.s.)jianfenglingensis Hua,新种(图1) 雌 体漆黑色,具光泽。上唇中央两侧,上颚外侧中央,额前缘及两侧缘,眼后颊部中央,触角基瘤内侧,眼后头顶中央,触角柄节下侧,梗节及第3节基部1/4,前胸背板中区两侧及侧刺突下方,小盾片,鞘翅上5组毛斑,前胸腹板中央两侧,中胸前侧片,     

中国天牛科二新记录属二新种(鞘翅目:天牛科，沟胫天牛亚科)

报道了中国天牛科二新记录属拟污天牛属Moechohecyra Breuning和瘤鞘天牛属Parachydaeopsis Breunning二新种;弧斑拟污天牛Moechohecyra arctifera sp.nov.和陕西瘤鞘天牛Parachydaeopsis shaanxiensis sp.nov.。新种模式标本保存于西南农业大学昆虫标本馆。     

中国纳天牛属二新种记述（鞘翅目：天牛科）

纳天牛属 Ｎｅｄｉｎｅ Ｔｈｏｍ ｓｏｎ, １８６４ 隶属于棒角天牛族 Ｒｈｏｄｏｐｉｎｉ,全球已知３种,分布于东南亚,我国尚无记录。该属的主要特征是触角显较体长,下沿有较密的缨毛,第２节与第４节近等长;体背相当拱隆略呈纺锤形;足较长,腿节呈棒状,后足腿节甚长,几伸达鞘翅末端,后足第１跗节近等长于第２、３节之和。新种模式标本保存于西南农业大学植物保护系昆虫标本馆。１． 刺翅纳天牛 Ｎｅｄｉｎｅ ｓｕｂｓｐｉｎｏｓａ, 新种 （图１～４）新种与 Ｎｅｄｉｎｅ ｌｏｎｇｉｐｅｓ Ｔｈｏｍ ｓｏｎ, １８６４ 较接近,主要区别为体红褐色而非灰褐色,鞘翅基部中央具１角状瘤突,鞘翅隐现３条灰白色绒毛横带而非被一色绒毛等。正模♂,云南付宁剥隘,１９７９－ Ⅴ－ ２,尹耀宜采;副模１♂,采集记录同正模。２． 麻斑纳天牛 Ｎｅｄｉｎｅ ｓｐａｒａｔｉｓ, 新种 （图５～８）本新种与刺翅纳天牛 Ｎ． ｓｕｂｓｐｉｎｏｓａ ｓｐ． ｎｏｖ． 的主要区别为体被均匀一致的绒毛,鞘翅无基瘤及淡色横带,触角下沿具更密而长的缨毛;雄性外生殖器的阳基侧突较短,中茎侧面中部具横凹陷等。正模♂,云南墨江,１９８０－ Ⅵ－ ５,刘芬采。     

中国脊筒天牛属分类研究(鞘翅目:天牛科)

系统整理了中国脊筒天牛属失一部种类,共31种,给出了所有种类的分类检索表,记述了新种暗背脊筒天牛Nupserha fuscodorsalis Wang et Chiang,sp nov。及2中国新纪录种三斑脊筒天牛Nupserha multimaculata Pic和黑肩脊筒天牛Nupserha nigrohumeralis Pic,新种正模保存于西南农业大学昆虫标本馆,副模共5号存于南开大学生物系标本室。     

黄斑星天牛与光肩星天牛的形态区别

成虫 黄斑星天牛A.nobilis G.与光肩星天牛A.globripennis M.除鞘翅的颜色深浅,光泽有无,小盾片和鞘翅上绒毛斑呈乳黄色或白色疏而大或密而小外,还可从雄性外生殖器上明显区别开来:黄斑星天牛的中茎长厚比为7.5,弯度较大,两侧向端部均匀渐狭,末端较圆。中茎突较狭。阳基侧突端部狭长,基部弯度不深。光肩星天牛的中茎长厚比为5.9,弯度较小,两边平行,直至近端部渐光圆。中茎突较宽阔。阳基侧突端部呈脚板状,基部弯度较深(图1—4)。     

中国霍克小蜂属—新种及—新记录（膜翅目：小蜂科）

1.木蛾霍克小蜂Hockeria epimactis新种 雌:体长5.0毫米。前翅基部1/3透明,端部稍褐,其余烟褐色,具暗褐色毛;近痣脉端部有1具白毛的圆斑,圆斑中有1褐色的钭纹;近臀角处另有1略呈三角形的大浅色斑(图1)。前、中足跗节及胫节端部暗黄褐色。胸部背面具白毛,其余毛黄白色。     

中国天牛科二新种(鞘翅目)

本文记述两个新种,均属于沟胫天牛亚科(Lamiinae)刺楔天牛属(Thermistis Pascoe)。模式标本保存于中国科学院动物研究所。 1.黄斑刺楔天牛Thermistis sulphureonotata,新种 体较长,底黑色,略带紫蓝色光泽,有硫黄色绒毛斑纹;唇基黑褐色;鞘翅缘角的尖刺呈红褐色。头部额区被硫黄色绒毛,触角第6至11节被灰黄色绒毛。前胸背板前端两侧     

Effects of photoperiod and low temperature on diapause termination in the yellow-spotted longicorn beetle (Psacothea hilaris)

Abstract. .The effects of photoperiod and low temperature on diapause termination in the yellow-spotted longicorn beetle, Psacothea hilaris (Pascoe) (Coleoptera: Cerambycidae), were examined using a population from Ino, Japan. Diapausing insects obtained by rearing larvae under short daylength (12 or 13 h) at 25^oC were subjected to various treatments. When the photoperiod was changed at the same temperature, diapausing larvae showed a long-day response with a critical daylength between 13.5 and 14h. The diapause was terminated and consequently pupation occurred if the daylength was longer than 13.5 h. Chilling the diapausing larvae at 10^oC for 30 or more days also terminated diapause in most larvae irrespective of the photoperiods during and after chilling treatment. In contrast, the post-chilling photoperiod had a critical effect on development of diapausing larvae chilled for only 15 days.     

Bumblebee Venom Serine Protease Increases Fungal Insecticidal Virulence by Inducing Insect Melanization

Insect-killing (entomopathogenic) fungi have high potential for controlling agriculturally harmful pests. However, their pathogenicity is slow, and this is one reason for their poor acceptance as a fungal insecticide. The expression of bumblebee, Bombus ignitus, venom serine protease (VSP) by Beauveria bassiana (ERL1170) induced melanization of yellow spotted longicorn beetles (Psacothea hilaris) as an over-reactive immune response, and caused substantially earlier mortality in beet armyworm (Spodopetra exigua) larvae when compared to the wild type. No fungal outgrowth or sporulation was observed on the melanized insects, thus suggesting a self-restriction of the dispersal of the genetically modified fungus in the environment. The research is the first use of a multi-functional bumblebee VSP to significantly increase the speed of fungal pathogenicity, while minimizing the dispersal of the fungal transformant in the environment.     

Diapause avoidance induced by low temperature in the yellow-spotted longicorn beetle, Psacothea hilaris

The diapause-averting effect of low temperature on pre-diapause larvae was examined in the yellow-spotted longicorn beetle, Psacothea hilaris. Larvae that had been reared under diapause-inducing conditions (25 °C , L12:D12) were temporarily exposed to 10 °C for various periods, and returned to the initial condition. Diapause was not averted by chilling for 15 days irrespective of the age of the larvae at chilling. After a 30-day chilling treatment, all of the 40- and 60-day-old larvae averted diapause, while diapause was averted in only one-third of the 10- and 20-day-old larvae. None of the pre-diapause larvae chilled for 60 days entered diapause irrespective of the age at chilling. With diapause avoidance, larvae that overwintered in earlier instars can start growing in earliest spring without any arrest; this phenomenon probably subserves the synchronization of larval development in a population.     

Change in significance of feeding during larval development in the yellow-spotted longicorn beetle, Psacothea hilaris

Larvae of the west-Japan type yellow-spotted longicorn beetle, Psacothea hilaris (Coleoptera: Cerambycidae), show a long-day type photoperiodic response at 25 degrees C; under long-day conditions, larvae pupate after the fourth or fifth instar, while under short-day conditions, they undergo a few nonstationary supernumerary molts and eventually enter diapause. In the present study, the effect of food on the development and photoperiodic response of the larvae was examined with special reference to molting and pupation. Although the pupal body size was greatly affected by the food quality and the length of feeding, the critical day length for induction of metamorphosis at 25 degrees C was always between 13.5 and 14 h. Exposure to starvation of larvae reared on the standard diet revealed that the capability to pupate is acquired after a few days of feeding in the fourth instar. In the larvae that had acquired the capability to pupate, premature pupation was induced by exposure to starvation, indicating that feeding becomes dispensable long before it is normally terminated.     

Feeding glucose or sucrose, but not trehalose, suppresses the starvation-induced premature pupation in the yellow-spotted longicorn beetle, Psacothea hilaris

Under 25 degrees C and a long-day photoperiod, starvation induces premature pupation in 4th instar Psacothea hilaris larvae exceeding a threshold weight of 180 mg, resulting in the formation of small but morphologically normal adults. To investigate possible mechanisms underlying this phenomenon, we first measured the hemolymph trehalose and glucose levels of starved larvae. When larvae were starved after 4 days of feeding (attaining the threshold weight), glucose levels decreased 4-fold within the next 24 h, while trehalose levels, after a temporary slight decrease, increased remarkably to reach a peak just before the prepupa stage. The effects of ingesting various nutrients on the developmental fate and the hemolymph sugar titers of starving larvae were then examined. Feeding on agar blocks containing sucrose or glucose totally suppressed the occurrence of premature pupation, while trehalose, fructose, casein and starch were ineffective. Feeding on glucose or trehalose resulted in a 6-fold decrease in hemolymph glucose levels and remarkably elevated trehalose levels. Since feeding on glucose and trehalose induced similar changes in hemolymph sugar titers but trehalose was not effective in suppressing premature pupation, glucose may have exhibited its effects via gustatory mechanisms.     

Photoperiodic control of larval diapause in the yellow-spotted longicorn beetle, Psacothea hilaris: analysis by photoperiod manipulation

The photoperiodic control of diapause induction in the larvae of the yellow-spotted longicorn beetle, Psacothea hilaris (Pascoe), was investigated using a west Japan-type population collected from Ino, Kochi Prefecture, Japan. In this population, the larvae expressed a long-day photoperiodic response with a critical daylength between 13.5 and 14 h at 25 °C ; under a long daylength, the larvae pupated after the 4th or 5th instar, while the larvae entered diapause under a short daylength after 2.3 additional molts on average. When the photoperiod was changed from a short (L12:D12) to a long (L15:D9) daylength, pupation occurred in most of the individuals irrespective of the time of the change. When the photoperiod was changed from long to short at 1 or 2 weeks after hatching, all of the larvae entered diapause, whereas when the photoperiod was changed at 5 weeks after hatching or later, most of the larvae pupated. The 2 weeks exposures to a long daylength against a 'background' of a short daylength at various times revealed that the larvae of this insect are most sensitive to the photoperiod from 4 to 6 weeks after hatching.     

Relationship between rapid cold-hardening and cold acclimation in the eggs of the yellow-spotted longicorn beetle, Psacothea hilaris

Rapid cold-hardening (RCH) and cold acclimation (ACC) were examined in eggs of the yellow-spotted longicorn beetle, Psacothea hilaris (Pascoe) (Coleoptera: Cerambycidae). When eggs incubated at 25 degrees C were transferred directly to conditions of -22 degrees C for 2h, less than 30% survived, whereas exposure to 0 degrees C for 4h prior to transfer to -22 degrees C increased survival to nearly 60%. The rapidly enhanced cold tolerance (RCH) was transient and lost rapidly after 1h at 25 degrees C. Incubation at 15.5 degrees C for 9 days (ACC) also enhanced cold tolerance. Comparison of the cold tolerance of non-treated eggs and eggs pre-treated to give RCH, ACC, or ACC+RCH allowed the relationship between the two hardening processes to be determined. At a mild subzero temperature (-10 degrees C) an RCH effect was not detected, whereas only RCH is effective at the severest subzero temperature just above the SCP (-26 degrees C). At intermediate temperatures (-16, -22 and -25 degrees C), ACC and RCH enhanced survival in combination. Therefore, the two hardening processes have different physiological bases but operate concomitantly over a wide temperature range.     

Antifungal properties and mode of action of psacotheasin, a novel knottin-type peptide derived from Psacothea hilaris

Psacotheasin is a 34-mer knottin-type peptide that is derived from Psacothea hilaris larvae. In this study, the antifungal activity and mechanism(s) by which psacotheasin affects human fungal pathogens were investigated. Psacotheasin shows remarkable antifungal properties without hemolytic activity against human erythrocytes. To understand the antifungal mechanism(s) of psacotheasin in Candida albicans, flow cytometric analysis with DiBAC₄(3) and PI was conducted. The results showed that psacotheasin depolarized and perturbed the plasma membrane of the C. albicans. Three-dimensional (3D)-flow cytometric contour-plot analysis, accompanied by decreased forward scatter (FS), which indicates cell size, confirmed that psacotheasin exerted antifungal effects via membrane permeabilization. The membrane studies, using a single GUV and FITC-dextran (FD) loaded liposomes, indicate that psacotheasin acts as a pore-forming peptide in the model membrane of C. albicans and the radius of pores were presumed to be anywhere from 2.3 to 3.3 nm. Therefore, the current study suggests that the mechanism(s) of psacotheasin’s antifungal properties function within the membrane.     

A method for rearing the yellow-spotted longicorn beetle, <Emphasis Type="Italic">Psacothea hilaris</Emphasis> (Coleoptera: Cerambycidae), to stabilize the last larval instar

The larvae of Psacothea hilaris (Pascoe) grown continuously under a long day in the laboratory pupate after the 4th, 5th, or 6th instar. This developmental polymorphism has complicated studies on the control of metamorphosis in P. hilaris. Since pupation in P. hilaris is known to be suppressed under a short day, a change in the photoperiod from a short to a long day with appropriate timing may assist in obtaining physiologically homogeneous larvae that pupate at the next molt. When the photoperiod was changed from 12-h light:12-h dark to 15-h light:9-h dark at the beginning of the 5th instar, the pupation rate at the next (5th) molt reached 90%, which was significantly higher than the proportion of larvae that pupated after the 5th instar when the larvae were grown continuously under a long day (52%). This rearing technique will expedite studies on the control of metamorphosis in P. hilaris.