共查询到20条相似文献,搜索用时 15 毫秒
1.
2.
Joanna Melonek Ruonan Zhou Philipp E. Bayer David Edwards Nils Stein Ian Small 《The Plant journal : for cell and molecular biology》2019,97(2):281-295
Nuclear restorer of fertility (Rf) genes suppress the effects of mitochondrial genes causing cytoplasmic male sterility (CMS), a condition in which plants fail to produce viable pollen. Rf genes, many of which encode RNA‐binding pentatricopeptide repeat (PPR) proteins, are applied in hybrid breeding to overcome CMS used to block self‐pollination of the seed parent. Here, we characterise the repertoire of restorer‐of‐fertility‐like (RFL) PPR genes in barley (Hordeum vulgare). We found 26 RFL genes in the reference genome (‘Morex’) and an additional 51 putative orthogroups (POGs) in a re‐sequencing data set from 262 barley genotypes and landraces. Whereas the sequences of some POGs are highly conserved across hundreds of barley accessions, the sequences of others are much more variable. High sequence variation strongly correlates with genomic location – the most variable genes are found in a cluster on chromosome 1H. A much higher likelihood of diversifying selection was found for genes within this cluster than for genes present as singlets. This work includes a comprehensive analysis of the patterns of intraspecific variation of RFL genes. The RFL sequences characterised in this study will be useful for the development of new markers for fertility restoration loci. 相似文献
3.
4.
5.
The chimeric mitochondrial gene orf182 causes non‐pollen‐type abortion in Dongxiang cytoplasmic male‐sterile rice 下载免费PDF全文
Hongwei Xie Xiaojue Peng Mingjuan Qian Yicong Cai Xia Ding Qiusheng Chen Qiying Cai Youlin Zhu Longan Yan Yaohui Cai 《The Plant journal : for cell and molecular biology》2018,95(4):715-726
6.
Mitochondrial–nuclear co‐evolution leads to hybrid incompatibility through pentatricopeptide repeat proteins 下载免费PDF全文
Mitochondrial–nuclear incompatibility has a major role in reproductive isolation between species. However, the underlying mechanism and driving force of mitochondrial–nuclear incompatibility remain elusive. Here, we report a pentatricopeptide repeat‐containing (PPR) protein, Ccm1, and its interacting partner, 15S rRNA, to be involved in hybrid incompatibility between two yeast species, Saccharomyces cerevisiae and Saccharomyces bayanus. S. bayanus‐Ccm1 has reduced binding affinity for S. cerevisiae‐15S rRNA, leading to respiratory defects in hybrid cells. This incompatibility can be rescued by single mutations on several individual PPR motifs, demonstrating the highly evolvable nature of PPR proteins. When we examined other PPR proteins in the closely related Saccharomyces sensu stricto yeasts, about two‐thirds of them showed detectable incompatibility. Our results suggest that fast co‐evolution between flexible PPR proteins and their mitochondrial RNA substrates may be a common driving force in the development of mitochondrial–nuclear hybrid incompatibility. 相似文献
7.
A genome‐scale analysis of mRNAs targeting to plant mitochondria: upstream AUGs in 5' untranslated regions reduce mitochondrial association 下载免费PDF全文
Timothée Vincent Audrey Vingadassalon Elodie Ubrig Kevin Azeredo Ola Srour Valérie Cognat Stéfanie Graindorge Thalia Salinas Laurence Maréchal‐Drouard Anne‐Marie Duchêne 《The Plant journal : for cell and molecular biology》2017,92(6):1132-1142
Intracellular sorting of mRNAs is an essential process for regulating gene expression and protein localization. Most mitochondrial proteins are nuclear‐encoded and imported into the mitochondria through post‐translational or co‐translational processes. In the latter case, mRNAs are found to be enriched in the vicinity of mitochondria. A genome‐scale analysis of mRNAs associated with mitochondria has been performed to determine plant cytosolic mRNAs targeted to the mitochondrial surface. Many messengers encoding mitochondrial proteins were found associated with mitochondria. These mRNAs correspond to particular functions and complexes, such as respiration or mitoribosomes, which indicates a coordinated control of mRNA localization within metabolic pathways. In addition, upstream AUGs in 5' untranslated regions (UTRs), which modulate the translation efficiency of downstream sequences, were found to negatively affect the association of mRNAs with mitochondria. A mutational approach coupled with in vivo mRNA visualization confirmed this observation. Moreover, this technique allowed the identification of 3'‐UTRs as another essential element for mRNA localization at the mitochondrial surface. Therefore, this work offers new insights into the mechanism, function and regulation of the association of cytosolic mRNAs with plant mitochondria. 相似文献
8.
9.
10.
11.
12.
13.
The E domains of pentatricopeptide repeat proteins from different organelles are not functionally equivalent for RNA editing 总被引:1,自引:0,他引:1
Anne‐Laure Chateigner‐Boutin Catherine Colas des Francs‐Small Sota Fujii Kenji Okuda Sandra K. Tanz Ian Small 《The Plant journal : for cell and molecular biology》2013,74(6):935-945
14.
An evolutionarily conserved P‐subfamily pentatricopeptide repeat protein is required to splice the plastid ndhA transcript in the moss Physcomitrella patens and Arabidopsis thaliana 下载免费PDF全文
Ayaka Ito Chieko Sugita Mizuho Ichinose Yoshinobu Kato Hiroshi Yamamoto Toshiharu Shikanai Mamoru Sugita 《The Plant journal : for cell and molecular biology》2018,94(4):638-648
15.
16.
17.
Empty pericarp7 encodes a mitochondrial E–subgroup pentatricopeptide repeat protein that is required for ccmFN editing,mitochondrial function and seed development in maize 下载免费PDF全文
Feng Sun Xiaomin Wang Géraldine Bonnard Yun Shen Zhihui Xiu Xiaojie Li Dahai Gao Zhonghang Zhang Bao‐Cai Tan 《The Plant journal : for cell and molecular biology》2015,84(2):283-295
18.
19.
20.
Ayoub Bouchoucha Florent Waltz Graldine Bonnard Mathilde Arriv Philippe Hammann Lauriane Kuhn Cdric Schelcher Hlne Zuber Anthony Gobert Philippe Gieg 《The Plant journal : for cell and molecular biology》2019,100(3):549-561
The essential type of endonuclease that removes 5′ leader sequences from transfer RNA precursors is called RNase P. While ribonucleoprotein RNase P enzymes containing a ribozyme are found in all domains of life, another type of RNase P called ‘PRORP’, for ‘PROtein‐only RNase P’, is composed of protein that occurs only in a wide variety of eukaryotes, in organelles and in the nucleus. Here, to find how PRORP functions integrate with other cell processes, we explored the protein interaction network of PRORP1 in Arabidopsis mitochondria and chloroplasts. Although PRORP proteins function as single subunit enzymes in vitro, we found that PRORP1 occurs in protein complexes and is present in high‐molecular‐weight fractions that contain mitochondrial ribosomes. The analysis of immunoprecipitated protein complexes identified proteins involved in organellar gene expression processes. In particular, direct interaction was established between PRORP1 and MNU2 a mitochondrial nuclease. A specific domain of MNU2 and a conserved signature of PRORP1 were found to be directly accountable for this protein interaction. Altogether, results revealed the existence of an RNA maturation complex in Arabidopsis mitochondria and suggested that PRORP proteins cooperated with other gene expression factors for RNA maturation in vivo. 相似文献