杏鲍菇液体培养研究初报

采用液体摇瓶培养方法,对杏鲍菇适宜的液体培养基和液全培养条件（适宜温度,摇瓶装量,摇瓶转速,接种量,培养基初始pH等）进行了探讨。结果表明,杏鲍 菇适宜的液体培养基为：葡萄糖3%,蛋白胨0.2%,KH2PO40.05%,MgSO40.05%,pH6.5,适宜的液全培养条件为：培养温度25℃,培养基的初始pH6.5,摇瓶装量100-140ml/500ml,摇瓶转速150r/min,接种量10%,最佳培养时间7d。     

Optimization of fermentation medium for Cordyceps militaris high producing strain

在单因素试验初步确定高产蛹虫草菌株发酵培养基的基础上,以蛹虫草菌丝体中腺苷含量为指标,进行11因素2水平Plackett - Burman试验设计试验,结合多元一次回归模型和F检验方法,筛选出发酵培养基中影响显著的组分酵母浸粉、蔗糖和维生素B1,采用旋转中心组合设计方法对这三个组分进行进一步优化,结合多元二次回归模型和响应面分析,获得高产蛹虫草菌株的最佳培养基(g/L):蔗糖18.85、蛋白胨10、酵母浸粉18.97、KH2 PO4 3、MgSO4 3、维生素B10.235、ZnCl2 0.011、(NH4)2SO4 10.验证试验结果表明蛹虫草腺苷得率较单因素优化获得的发酵培养基提高了26.91％.     

高产蛹虫草菌株发酵培养基的研究

在单因素试验初步确定高产蛹虫草菌株发酵培养基的基础上,以蛹虫草茵丝体中腺苷含量为指标,进行11因素2水平Plackett—Burman试验设计试验,结合多元一次回归模型和F检验方法,筛选出发酵培养基中影响显著的组分酵母浸粉、蔗糖和维生素B1,采用旋转中心组合设计方法对这三个组分进行进一步优化,结合多元二次回归模型和响应面分析,获得高产蛹虫草菌株的最佳培养基（g／L）：蔗糖18．85、蛋白胨10、酵母浸粉18．97、KH2PO,3、MgSO4 3、维生素Bl0．235、ZnCl20．011、（NH4）2S0410。验证试验结果表明蛹虫草腺苷得率较单因素优化获得的发酵培养基提高了26．91％。     

深红虫草高产卵孢菌素的培养基筛选及发酵条件优化

本研究以提高深红虫草Cordyceps cardinalis C033次级代谢产物卵孢菌素的产量为目标,对其产卵孢菌素的培养基进行筛选,并利用单因素和正交试验对深红虫草产卵孢菌素的发酵条件进行了优化。结果表明,深红虫草产卵孢菌素的最适发酵培养基为马铃薯‐蛋白胨‐葡萄糖培养基,最佳发酵参数为:发酵时间6d,培养基初始p H 7.0,接种量6%,培养温度24℃,每500m L锥形瓶装液量100m L,摇瓶转速120r/min。用最佳培养基进行发酵条件优化后获得卵孢菌素448.70μg/m L,产量是优化前的10.14倍。该研究结果为菌株C033在农业害虫防治上的应用奠定了基础。     

蛹虫草液体培养条件的优化及生长动力学考察

为了优化蛹虫草菌的液体培养条件 ,对液体培养菌丝的生长情况进行了研究。采用摇瓶培养的方法 ,以发酵得率为指标确定培养条件。结果表明 :优化培养基成分确定为蛋白胨 1.5 % ,葡萄糖 2 % ,KH2 PO4 0 .15 % ,Mg SO40 .0 5 %、VB1 5 m g/ L .2 ,4 - D 2 mg/ L .该培养基下发酵得率达 1.4～ 2 .2 g/ 10 0 m L ;考察了蛹虫草菌液体培养的外部条件 ,确定为 :装料系数为 30 % ,培养基 p H为 5 .5 ,接种量为 0 .0 3g,摇床振荡频率为 12 0 r· m in- 1 ;利用优化培养基得到了生长代谢曲线。在相同条件下 ,优化培养基比原来培养基的发酵得率提高了 4 .5 % ,研究初步得到蛹虫草菌液体培养条件和生长动力学 ,为工业化生产提供了一定的依据     

金针菇FV908菌株液体培养工艺的研究

通过单因子试验统计分析 ,优化筛选了适于金针菇 (Flammulinavelutipes)FV90 8的适宜培养基和摇瓶培养条件 ,结果表明 ,其适宜的液体培养基组成为玉米粉 5 .0 % ,麸皮 2 .0 % ,KH2 PO4 0 .1% ,MgSO4 ·7H2 O 0 .0 5 % ,10 μgVB1/ 10 0mL ,5 0 μgVB2 / 10 0mL ;适宜的摇瓶培养条件为 :培养基的起始pH 6 .0～ 7.0 ,5 0 0mL摇瓶装量为15 0mL ,接种量为 10 % ,培养温度 2 5℃ ,摇床转速为 12 0r/min ,菌丝干收率 39g/L。     

一株竹黄无性型菌株液态发酵产竹红菌素的初步研究

从野生竹黄子座中分离获得能产生竹红菌素的无性型菌株ZH-5-1,经液态发酵培养及摇瓶正交试验确定最佳培养基配方和培养条件:葡萄糖30g/L,蛋白胨5g/L,NaNO3 10g/L,KCl 1.5g/L,MgSO4 1.5g/L,KH2PO4 2g/L,pH 值6.0,装液量100/250 mL(V/V),接种量10%(V/V),培养温度28℃,摇床转速130 r/min,培养周期96h.     

黑曲霉X-1产木聚糖酶液体发酵工艺研究

目的:提高黑曲霉X-1菌株液体发酵木聚糖酶的产量.方法:采用单因素和正交试验对黑曲霉X-1菌株产木聚糖酶液体发酵培养基和培养条件进行了优化.结果:通过正交试验找出最大影响因素为玉米芯和葡萄糖的供应,优化后的最佳培养基和培养条件为:玉米芯粉3%,葡萄糖0.5%,蛋白胨1%,KH2PO4 0.5%,MgSO4·7HO 0.1%,Tween-80 0.‰,pH 6.5,30℃、200r/min摇瓶培养120h.结论:黑曲霉X-1菌株在优化后的培养基和培养条件下,木聚糖酶活力高达1 630.78U,比优化前木聚糖酶活力(724.63U)提高了125.05%.     

蔗渣发酵菌剂培养基的筛选及发酵条件的优化

采用单因素和正交试验研究了蔗渣高效发酵菌剂(芽孢杆菌B-A、曲霉菌F-A、链霉菌A-B)的摇瓶发酵最佳工艺条件.结果表明:芽孢杆菌B-A的最佳培养基配方:牛肉膏0.3%、蛋白胨1%、葡萄糖1%、NaCl 0.5%、可溶性淀粉0.5%、3.08%浓度的MnSO4溶液0.1;最适发酵条件为pH7、装液量100 ml(250 ml三角瓶)、36℃培养27 h.曲霉F-A的最佳培养基配方:葡萄糖3%、豆饼粉3%、蛋白胨1.2%、酵母膏0.3%、K2HPO4 0.05%、KH2PO40.05%、CaCl20.08%、MgSO40.04%、MnSO40.04%、ZnSO40.02%;最适发酵条件为pH6、装液量50 ml、30℃培养3 d.链霉菌A-B的最佳培养基配方:可溶性淀粉4.5%、蔗糖1%、豆饼粉3%、NaNO30.2%、ZnSO40.01%、KH2PO40.001%;最适发酵条件为pH7、装液量50 ml、30℃培养3 d.     

5′—磷酸二酯酶高产菌株的选育和发酵培养条件的优点

以ATCC14994为出发菌株,采用紫外线与亚硝基胍相结合的多次诱变育种,获得1株5′-磷酸二酯酶高产菌株HAT2228.通过单因子和正交试验对该菌株的产酶发酵条件进行了优化,优化发酵产酶条件为蔗糖5%,酵母膏0 3%,蛋白胨0.3%,K2HPO4 0.8%,KH2PO40.8%,MgSO4 0.2%,ZnSO4 0.2%,培养基起始pH6 0,接种量10%,培养温度30℃,摇床转速120r/min,发酵时间48h.在优化条件下,HAT2228的产酶水平达1 329u/ml.     

桑黄液体发酵工艺的研究

通过对桑黄液体发酵培养基、培养条件优化实验研究,以获得具有与桑黄子实体相似功效成分的桑黄菌丝体液体发酵工艺。以菌丝体收率为主要考察指标,采用单因子及L9(34)正交实验的方法,对桑黄液体发酵培养基及培养条件进行优化,确定桑黄液体发酵工艺条件。桑黄液体发酵最佳培养基及培养条件:玉米粉2%,葡萄糖3%,酵母膏0.5%,蛋白胨0.5%,KH2PO40.3%,Mg SO4·7H2O 0.15%,VB120μg/100 m L,p H5.5,接种量8%,培养温度28℃,摇床转数180 r/min,培养周期82 h。优化条件下所获得桑黄菌丝体粉为土黄色,菌丝体平均得率为1.67%,菌丝体黄酮含量(0.84%)与桑黄子实体(0.88%)相当,菌丝体多糖含量(5.15%)是子实体(1.71%)的3倍。可见,该桑黄液体发酵工艺具有较大的推广应用价值。     

桔青霉生产核酸酶的发酵条件研究

对通过紫外线和60Co两次育种得到的桔青霉W48高产菌株培养基的单组分和发酵条件进行了优化,最后又用正交实验对培养基的各组分的浓度进行了优化。得到了产酶量最高的培养基组分(质量分数)是:KH2PO40.05%,K2HPO40.03%,酵母膏+蛋白胨0.7%,CaCl20.02%,MgSO40.04%,ZnSO40.03%,葡萄糖5%,pH5.5。最佳发酵条件是:接种量10%,装液量50 mL,摇床转速180 r/min,温度30℃,发酵时间66 h。用最佳培养基和最佳发酵条件发酵生产核酸酶的酶活力为758.10 U/mL,原始菌种产核酸酶的酶活力为272.26 U/mL,提高了2.78倍。     

Ethanol production from sugar cane segments in a high solids drum fermentor

Summary A successful yeast fermentation for the production of relatively high concentration of ethanol (9% w/v) was carried out using sugar cane segments. Extraction of sugar from segments occurred simultaneously with ethanol formation. The beer produced was transferred to a fresh batch of sugar cane segments and the fermentation cycle was repeated successively three times with the same beer. A high cane to water ratio was obtained in a rotating drum fermentor which allowed for a minimal amount of liquid to be used during the fermentation process.     

生防菌Ⅲ-61抗真菌活性产物的摇瓶发酵

对生防链霉菌Ⅲ-61产生抗真菌活性物质的摇瓶发酵工艺进行了研究。利用正交试验设计优化了发酵培养基组分,其最适配方为黄豆粉1．5％,蛋白胨0．3％,蔗糖1．0％,淀粉1．3％,磷酸二氢钾0．02％,硫酸镁0．025％,氯化钠0．5％,配咸水溶液,调pH至7～7．4,加碳酸钙1％。通过单因素试验,筛选获得了最优培养条件组合：液体种龄24h,接种量5％～10％,500mL摇瓶培养基装量为80mL,摇床转速240r／min,培养温度31℃,发酵周期96～120h。此优化的发酵培养基与发酵条件的组合昕得菌株Ⅲ-61发酵液对主要靶标黄瓜灰霉病菌的抑菌圈直径达49．5mm,较优化前提高了45．59％。     

产漆酶真菌的筛选及其固态发酵条件研究

用愈创木酚平板法对14株白腐真菌进行初筛,通过测定漆酶活力进行复筛,筛选出1株生活力较强,产漆酶活力高的菌株MZ-1,经ITS-5.8S rDNA序列分析,初步鉴定为Trametes versicolor。在固态发酵培养基的基础上,对该菌株产漆酶的培养基组成进行正交优化,得到最优发酵培养基:麸皮∶秸秆粉∶豆粕∶玉米粉为3∶3∶2∶1,可溶性淀粉2%,(NH4)2SO4+蛋白胨1%,KH2PO40.1%,料水比1∶2。接种4个菌塞,温度为30℃,发酵8 d后酶活可达到1 555.57 U/g。     

Production of fuel ethanol at high temperature from sugar cane juice by a newly isolated Kluyveromyces marxianus

Kluyveromyces marxianus DMKU 3-1042, isolated by an enrichment technique in a sugar cane juice medium supplemented with 4% (w/v) ethanol at 35 degrees C, produced high concentrations of ethanol at both 40 and 45 degrees C. Ethanol production by this strain in shaking flask cultivation in sugar cane juice media at 37 degrees C was highest in a medium containing 22% total sugars, 0.05% (NH(4))(2)SO(4), 0.05% KH(2)PO(4), and 0.15% MgSO(4).7H(2)O and having a pH of 5.0; the ethanol concentration reached 8.7% (w/v), productivity 1.45 g/l/h and yield 77.5% of theoretical yield. At 40 degrees C, a maximal ethanol concentration of 6.78% (w/v), a productivity of 1.13 and a yield 60.4% of theoretical yield were obtained from the same medium, except that the pH was adjusted to 5.5. In a study on ethanol production in a 5l jar fermenter with an agitation speed of 300 rpm and an aeration rate of 0.2 vvm throughout the fermentation, K. marxianus DMKU 3-1042 yielded a final ethanol concentration of 6.43% (w/v), a productivity of 1.3g/l/h and a yield of 57.1% of theoretical yield.     

产腈水解酶基因工程菌的产酶诱导条件及培养基优化

本文通过对产酶诱导条件及发酵培养基进行优化,成功提高了产腈水解酶基因工程菌E. coli BL21(DE3)-pETNYNit的产酶水平。研究结果显示,最佳发酵培养基为：葡萄糖0.2%、甘油0.7%(v/v)、蛋白胨1.2%、酵母膏0.8%、NaCl 0.3%、(NH4)2SO40.3%、NH4Cl 0.13%、Na2 HPO4·12H2 O 1.04%、KH2 PO40.39%、MgSO4·7H2 O 0.03%,pH 7.2。最佳产酶诱导条件为：发酵4 h时加入0.5 mmol/L IPTG,然后在28℃、240 r/min下诱导腈水解酶基因表达14 h~16 h。采用优化方案,重组菌产酶水平可提升至0.9~1×105 U,与野生菌株的产酶水平相比,提高幅度超过50%。同时重组菌培养仅需24 h,培养周期缩短超过50 h。     

Optimization of fermentation medium and conditions for mycelial growth and water-soluble exo-polysaccharides production by Isaria farinosa B05

The optimal fermentation medium and conditions for mycelial growth and water-soluble exo-polysaccharides production by Isaria farinosa B05 were investigated. The medium components and fermentation conditions were optimized according to the one at a time method, while the concentration of medium components was determined by the orthogonal matrix method. The results showed that the optimal fermentation medium was as follows: sucrose 3.5% (w/v), peptone 0.5%, yeast extract 0.2%, K(2)HPO(4) 0.1%, and MgSO(4) 0.05%. The suitable fermentation conditions were as follows: initial pH 7.0, temperature 25 degrees C, medium volume 75 mL/250 mL, inoculum volume 5% (v/v), time 5d. In such optimal nutrition and environmental conditions, the maximal mycelial yield was 2.124 g/100 mL after 4 day's fermentation, while maximal water-soluble exo-polysaccharides production reached 2.144 g/L after 5 day's fermentation.     

Solid‐state fermentation for the production of debittering enzyme naringinase using Aspergillus niger MTCC 1344

Aspergillus niger produced high levels of naringinase using easily available, inexpensive industrial waste residues such as rice bran, wheat bran, sugar cane bagasse, citrus peel, and press mud in solid‐state fermentation (SSF). Among these, rice bran was found to be the best substrate. Naringinase production was highest after 96 h of incubation at 27°C and at a substrate‐to‐moisture ratio of 1:1 w/v. Supplementation of the medium with 10% naringin caused maximum induction. An inoculum age of 72 h and an inoculum level of 15% resulted in maximum production of naringinase. Enzyme production was stimulated by the addition of nutrients such as naringin and peptone. Thus, A. niger produced a very high level of naringinase within a short time in solid‐state fermentation using inexpensive agro‐residues, a level that is much higher than reported for any other microbes.