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1.
影响籼稻愈伤组织再生频率的几个因素   总被引:3,自引:0,他引:3  
本文研究了影响湘早籼19号愈伤组织植株再生频率的几个因素。在诱导培养基中添加不同配比的细胞分裂素(KT,BAP,Zeatin)和萘乙酸,可使再生频率大幅度提高,以补加Zeatin和NAA效果最为显著,达25.33%;诱导培养基和分化培养基的不同琼脂浓度组合处理,以诱导培养基0.75%琼脂和分化培养基1%琼脂与诱导培养基1%琼脂与分化培养基0.5%琼脂两组合再生频率最高。同时还发现:诱导分化后转移至植株生长培养基也可提高再生率,而诱导培养基中补加脯氨酸的合适浓度是50mg/L。  相似文献   

2.
植物组织培养中的玻璃化现象及其预防   总被引:21,自引:0,他引:21  
玻璃化是植物组织培养中常见的一种现象。本从形态解剖和生理生化方面比较了玻璃化苗和正常植株的差异。围绕水势平衡、激素平衡和矿质元素平衡探讨了玻璃化发生的内在机制,在此基础上总结了防止玻璃化发生的可能途径:改善光照,选用透气封口膜,降低细胞分裂素的浓度等,并提出多学科的合作将是防止玻璃化发生的必由之路。  相似文献   

3.
某些因素对玻璃苗形成的影响和玻璃苗在形态解剖上的特点   总被引:11,自引:0,他引:11  
瑞香试管苗的玻璃化受到细胞分裂素浓度和茎尖外植体划、的影响,细胞分裂素浓度越高,茎尖外植体越小,产生的玻璃苗越多,玻璃苗与正常试管苗的茎尖在形态结构上有明显差别,主要表现为顶端分生组织原体原套结构异常和细胞的明显液泡化。  相似文献   

4.
对应用突变体研究细胞分裂素信号转导、细胞分裂素受体以及参与细胞分裂素信号转导相关的蛋白质作了简要介绍;并且对细胞分裂素信号途径进行了推测:细胞分裂素被受体(CKI1、IBC6或者GCR1)接受后,经传导系统转化形成特定的信息,一方面可能调节基因的表达,从而可能调节受体水平,导致细胞对细胞分裂素浓度应答范围发生改变,另外,基因表达使细胞产生相关的生理反应;另一方面形成的特定信息可能激活MAPK级联途径(mitogen-activated protein kinase cascade),导致细胞产生相关的生理反应。  相似文献   

5.
报告了一些重要因子对培养在含有乙醇的培养基上的烟草细胞的乙醇分解代谢能力(ECA)的调控效果。在供试的5种生长毒和2种细胞分裂素中,IBA 4mg/L和Kt0.05mg/L最有地促进ECA,而NAA2mg/L和Kt0.025mg/L则最有利于支持处在乙醇胁迫下的细胞的增殖。实验中还发现提高维生素浓度有利于细胞的ECA。通过将细胞从低乙醇浓度的培养基转移到较高乙醇浓度的培养基中,能够诱导细胞耐受更高  相似文献   

6.
瑞香试管苗的玻璃化受到细胞分裂素浓度和茎尖外植体大小的影响,细胞分裂素浓度越高,茎尖外植体越小,产生的玻璃苗越多。玻璃苗与正常试管苗的茎尖在形态结构上有明明显差别,主要表现为顶端分生组织原体原套结构异常和细胞的明显液泡化。  相似文献   

7.
报告了一些重要因子对培养在含有乙醇的培养基上的烟草细胞的乙醇分解代谢能力(ECA)的调控效果。在供试的5种生长素和2种细胞分裂素中,IBA4mg/L和Kt0.05mg/L最有利于促进ECA,而NAA2mg/L和Kt0.025mg/L则最有利于支持处在乙醇胁迫下的细胞的增殖。实验中还发现提高维生素浓度有利于细胞的ECA。通过将细胞从低乙醇浓度的培养基转移到较高乙醇浓度的培养基中,能够诱导细胞耐受更高浓度的乙醇并增强细胞的ECA。研究结果可以应用于制备适合分离乙醇分解代谢途径中的酶的烟草细胞材料。  相似文献   

8.
不同培养条件对薄荷试管苗玻璃化现象的影响   总被引:10,自引:2,他引:8  
以江苏东台产薄荷(M entha haplocalyxB riq.)的茎尖为外植体,以MS为基本培养基,研究了培养基添加物和培养条件对薄荷试管苗玻璃化现象的影响。结果显示,导致薄荷玻璃化苗产生的主要因素是培养基中的6-BA、蔗糖和琼脂浓度以及培养温度和光照时间;当6-BA浓度为2 mg.L-1、蔗糖浓度为4%、琼脂浓度为0.70%、培养温度25℃、光照12 h.d-1(2 000 lx)时,薄荷试管苗的繁殖系数较高,玻璃化苗率较低。  相似文献   

9.
甜瓜离体再生继代培养中玻璃化现象的研究   总被引:4,自引:1,他引:3  
为提高甜瓜离体培养的再生率和转基因效率,以优质甜瓜品种‘伽师瓜’(‘卡拉库赛’)离体再生不定芽为外植体,通过连续多代继代培养,对引起玻璃化苗现象的几个主要因素进行了研究。结果表明,在甜瓜离体再生继代培养中,外植体继代次数是影响玻璃化发生的主要因素,同时培养基中的6-BA浓度偏高、琼脂浓度偏低以及蔗糖浓度偏低或偏高等可导致玻璃化苗的增加。培养基中较低的6-BA浓度(0~0.2 mg/L),琼脂浓度为6 g/L,蔗糖浓度为25 g/L以及添加活性炭等措施可有效地降低甜瓜玻璃化苗的发生。  相似文献   

10.
李海刚  孔祥生 《生物学杂志》2010,27(5):35-37,42
以MS为基本培养基,研究品种、琼脂、6-BA、大量元素、蔗糖、光照等因素对牡丹试管苗玻璃化的影响。结果显示:不同品种的牡丹试管苗在组织培养的过程中,出现不同程度的玻璃化,增加培养基中琼脂浓度、降低细胞分裂素6-BA的浓度、降低大量元素的浓度和增加光照强度或采用自然光照均可以有效地降低试管苗玻璃化。  相似文献   

11.
重瓣丝石竹茎尖培养快繁技术的研究结果表明:适宜的芽增殖培养基为MS+A1.0~2.0mg/L+NAA0.2~1.0mg/L或MS+IBA0.5~1.0mg/L;生根培养基为MS+NAA0.03~0.05mg/L;白糖代替蔗糖对菌的增殖和生根无影响;液体培养基可获得和琼脂培养基相同的增殖效果;不同材料的增殖效果有明显的差异。  相似文献   

12.
液体悬浮培养促进铁皮石斛原球茎高效诱导、增殖的研究   总被引:2,自引:0,他引:2  
用正交设计方法对铁皮石斛原球茎具有高效增殖影响的激素(BA,NAA,KT,马铃薯汁)以及配比进行研究,结果表明:以茎段为外植体在培养基Ms+BA2.0mg/L+NAA2.0mg/L+马铃薯汁10%+糖3%,具有很好的原球茎诱导作用,50d后诱导率达95.20%;原球茎在1/2MS+BA2.0mg/L+NAA1.0mg/L+KT0.5mg/L+糖3%的培养基上,以液体悬浮培养,原球茎增殖达49.032g/50d。  相似文献   

13.
不同激素对匙叶芋芽的诱导与增殖的影响   总被引:1,自引:0,他引:1  
取日本引进的Spathiphyllum sp.根茎外植体接种在不同激素配比的MS培养基上,结果以MS BA 2mg/l KT 1mg/l诱导芽的效果最好;通过附加不同种类的细胞分裂素,不同浓度的BA,不同生长素的试验,证明MS BA 1mg/l IAA 0.1mg/l组成较有利于芽的增殖;将芽移入生根培养基,15天左右长出根,形成完整植株。  相似文献   

14.
Asada M  Ishibashi S  Ikumi S  Fukui Y 《Theriogenology》2002,58(6):1199-1208
Polyvinyl alcohol (PVA) was used as a substitute for serum in a vitrification solution for in vitro matured bovine oocytes. In vitro matured bovine oocytes were cryopreserved in various vitrification solutions (VS) supplemented with different concentrations (0.05, 0.1, 0.5, and 1%) of PVA, 20% fetal calf serum (FCS) or without macromolecule supplementation in a gel-loading tip (GL-tip). After warming, vitrified oocytes were examined for effects on survivability, fertilizability, and embryonic development in vitro. At 18 h in vitro fertilization after vitrifying and warming, the number of surviving mature oocytes vitrified in VS without macromolecule supplementation was significantly (P < 0.05) lower than those with macromolecule supplementation. For fertilizability after vitrification, there was no significant difference in the penetration rate of oocytes among fresh oocytes (98.7%); oocytes vitrified in VS supplemented with 0.1 (76.8%), 0.5 (70.2%), or 1% (80.3%) PVA; 20% (84.1%) FCS; or without supplementation (61.7%). Also, the normal fertilization rate was not significantly different in oocytes vitrified with 0.1 (56.5%), 0.5 (43.5%), or 1% (49.7%) PVA and 20% (60.6%) FCS, compared with fresh oocytes (84.0%). Subsequently, vitrified oocytes were examined for embryonic development effects in vitro. The highest proportion of cleaved oocytes after vitrification was obtained in VS supplemented with 0.1% (18.8%) PVA. Additionally, the proportion of development to morula stage (7.7%) in the oocytes vitrified in a VS supplemented with 0.1% PVA was significantly (P < 0.05) superior to that of the 0, 0.5, and 1% PVA-vitrified groups. However, the beneficial effect of PVA addition was not found in blastocyst development. Embryonic development of vitrified oocytes was significantly lower than that of fresh oocytes. In conclusion, the present results indicate that 0.1% PVA supplementation in VS results in a significantly higher rate of morula stage embryos than 0, 0.5, and 1% PVA supplementation, and could replace FCS in VS for vitrification of in vitro matured bovine oocytes.  相似文献   

15.
石刁柏,又名芦笋(Asparagus officinalisL.)是百合科天门冬属植物。其栽培品种含有丰富的维生素类及蛋白质。同时,石刁柏对于某些疾病有一定的药效,因此它已成为人们所喜爱的一种高级营养蔬菜。国外已有不少关于石刁柏试管苗繁殖的报告,但至今只有Bui Dang Ha等从石刁柏枝状叶分离的原生质体得到愈伤组织,并由此愈伤组织诱导获得了再生植株。此后,未见在石刁柏的原生质体培养方面再有新的工作。在本文中,我们利  相似文献   

16.
Summary Vitrification of plants in vitro is a physiological abnormality of tissue-cultured plants which causes significant losses in the micropropagation industry. Vitrified plants are waterlogged but the position of water within plants has not been identified. Nuclear magnetic resonance (NMR) imaging of normal tissue-cultured, vitrified tissue-cultured, and glasshouse-grown leaves ofGypsophila paniculata showed the distribution of water within the leaves. Normal tissue-cultured and glasshouse-grown leaves had a high concentration of water within leaf vascular bundles and lower concentrations elsewhere. In contrast, vitrified leaves had a relatively even distribution of high water concentration throughout the leaves. When imaging parameters were changed, so that only water associated with cell membranes was shown, the images of normal tissue-cultured and glasshouse-grown leaves did not change. However, the image of the vitrified leaves showed a general lowering of intensity across the whole of the leaf. The appearance of the NMR images, together with those obtained by light microscopy, suggest that the excess water associated with vitrified plants is located in the intercellular air spaces. The blockage of these spaces may lead to a cycle of perturbations in the plant's physiology culminating in the development of vitrification.Abbreviation NMR nuclear magnetic resonance  相似文献   

17.
The optimum physical and chemical microenvironment for micropropagation of Limonium sinensis (Girard) Kuntze, var. Golden Diamond was established from immature inflorescence segments as explant. The highest frequency (62 %) of axillary shoot induction was obtained on MS medium (Murashige and Skoog Physiol Plant 15:473–497, 1962) supplemented with 8.88 μM BA, 1.34 μM of NAA and two growth additives cysteine (142.33 μM), and glutamine (684.22 μM). In the subsequent culture maximum average number of shoots (11.13?±?0.34) were obtained from micro-shoot explant on MS medium supplemented with the same additives and 2.22 μM BA. During subcultures the problem of vitrification was mitigated through increasing agar concentration from 0.8 % to 1.0 % and providing better ventilation. The in vitro developed shoots were rooted on MS medium supplemented with 2.46 μM IBA and 0.88 μM BA. Rooted plants were acclimatized successfully in the greenhouse with 80 % survival rate. RAPD analysis using 15 random decamer primers generated monomorphic banding pattern in micropropagated plants and similar to those of mother plant revealing the genetic integrity of regenerants.  相似文献   

18.
海石竹的离体快繁及核型分析   总被引:2,自引:0,他引:2  
以海石竹 (Armeriamaritima)的叶片为外植体 ,经离体培养诱导产生愈伤组织 ,再分化形成不定芽 ,并经过继代增殖和壮苗生根 ,获得完整的再生植株 ,最后对其再生植株进行核型分析。结果表明 ,海石竹叶片的愈伤组织诱导和分化的适宜培养基为MS +BA 1 .0mg/L +NAA 0 .2mg/L ,诱导初期进行 7d暗培养 ,最佳增殖培养基为MS+BA 1 .0mg/L +NAA 0 .1mg/L ,生根培养基为MS+NAA 0 .2mg/L。以上培养基均含蔗糖3 0 g/L ,琼脂 5g/L ,pH 5 .8。海石竹的核型公式为 2n=2x=1 8=1 0m +8sm ,存在染色体数目变异的现象。  相似文献   

19.
以极东锦鸡儿未成熟合子胚子叶为外植体进行其体细胞胚胎发生和植株再生研究。在添加不同BA与NAA或2,4-D,外加500mg·L~(-1)水解酪蛋白、30g·L~(-1)蔗糖和8g·L~(-1)琼脂的MS培养基上诱导产生了体细胞胚。在5mg·L~(-1)NAA+5mg·L~(-1)BA和5mg·L~(-1)2,4-D+1mg·L~(-1)BA处理中体胚诱导率分别为14%和10%;NAA处理每外植体上诱导出的体胚数量最多为4.3个,而2,4-D为10.5个。体细胞胚经成熟培养后,在添加0.01mg·L~(-1)NAA、20g·L~(-1)蔗糖和6g·L~(-1)琼脂的MS培养基上萌发率达到58.94%。萌发的体胚在MS培养基上长成正常小植株,再生率为87%。经炼苗后的体胚苗移植到草炭土:蛭石:珍珠岩=5:4:1(V/V/V)的栽培基质中,可以正常生长,移栽成活率为40%。  相似文献   

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