中国木生革菌研究4.刺囊革菌属，二叉韧革菌属和叉丝革菌属中的三个中国新记录种

报道中国木生革菌3个新记录种：艾氏刺囊革菌采自河南省,褐伏二叉韧革菌采自安徽省,柳叉丝革菌采自贵州省。根据所采集的标本对这3种真菌进行了详细描述。     

锈革菌属三个中国新记录种

报道了锈革菌属Hymenochaete 3个中国新记录种。双孢锈革菌Hymenochaete bispora采自吉林省,担子大多数有两个担子梗;柔软锈革菌Hymenochaete lenta采自内蒙古自治区,子实体覆瓦状叠生且担孢子呈尿囊形;暗赭色锈革菌Hymenochaete senatoumbrina采自西藏自治区,属于裸刚毛组中孢子较大的种类。文中对这3个种进行了详细的描述和显微结构绘图。     

中国锈革菌属研究3.采自广西和吉林的三个中国新记录种(英文)

报道了锈革菌属 Hymenochaete 3 个中国新记录种。圆孢锈革菌 H. globispora 和莱热锈革菌 H. legeri 采自广西自治区,榆锈革菌 H. ulmicola 采自吉林省。圆孢锈革菌的主要特点是孢子宽椭圆形或近球形,属于锈革菌组;莱热锈革菌子实体灰白色,刚毛具结晶,孢子圆柱形,属于裸刚毛组;榆锈革菌生长在活的榆树树皮上,属于锈革菌组。对这 3 个种进行了详细的描述和显微结构绘图。     

中国锈革菌属研究1.采自广东和安徽的三个中国新记录种

报道了锈革菌属Hymenochaete 3个中国新记录种。贝尔泰罗锈革菌H. berteroi和极薄锈革菌H. tenuis采自广东省,塔斯马尼亚锈革菌H. tasmanica采自安徽省。贝尔泰罗锈革菌的主要特点是菌丝近念珠状,具刚毛状菌丝,且孢子较小;极薄锈革菌属于裸刚毛组,子实体薄且孢子窄椭圆形或短圆柱形;塔斯马尼亚锈革菌菌肉分层且子实层中存在树状侧丝。文中对这3个种进行了详细的描述和显微结构绘图。     

中国木生革菌研究Ⅰ.簇生针齿菌和离心射脉革菌(英文)

簇生针齿菌和离心射脉革菌2种木腐菌是中国新记录种。簇生针齿菌采自河南省,湖南省和云南省,该种与金黄针齿菌比较相似,但是后者的子实层体表面金黄色,担孢子较宽。离心射脉革菌采自吉林省长白山自然保护区,该菌与辐射射脉革菌比较接近,但是后者具有囊状体和较小的担孢子。本文根据采集的材料对这2个种进行了详细的描述和显微结构绘图。     

锈革菌属两个中国新记录种

报道了锈革菌属Hymenochaete两个中国新记录种:长孢锈革菌H.longispora采自广东省,主要特点是孢子长圆柱形且刚毛较大;微孢锈革菌H.nanospora采自海南省和广东省,主要特点是刚毛和孢子非常小。文中提供了这两个种的野外标本照片、显微结构绘图以及详细的形态学描述。     

中国木生革菌研究3.原毛平革属

本文报道了三种木生革菌:南方原毛平革菌Phanerochaete australis,纤毛原毛平革菌P.calotricha和革质原毛平革菌P.stereoides,分别采自河南省,吉林省和湖北省。前两种是中国新记录种,最后一种为中国大陆首次报道。本文根据采集到的标本对它们进行了详细的描述和绘图,并给出了我国该属已知19种的分种检索表。     

中国革菌3新记录种

本文报道了中国革菌3个新记录种:灰白片花耳采自湖南省,其担子上着生两个担孢子梗。匙形茸瑚菌采自云南省,其担子果具菌盖、侧生柄,菌丝二系。硬锈红菌采于海南省,其平伏,灰褐色至暗褐色的子实层体可使附着基物变红。本文根据所采集的标本对这3种真菌进行了详细的描述。     

海南霸王岭自然保护区多孔菌研究(英文)

对采自海南霸王岭自然保护区的木生多孔菌约400号标本进行了鉴定共获得110种。按字母顺序列出了这些多孔菌名录;其中浅黄林氏革孔菌Lindtneria flava为中国新记录种,提供了详细描述和显微结构绘图;同时,依据文献对世界林氏革孔菌属种类进行了总结,并提供了该属所有种类的检索表。     

中国锈革孔菌科两新记录种

本文报道了中国秀革孔菌科两个新记录种,路易斯安纳纤孔菌Inonotus ludovicianus和金黄木层孔菌Phellinus chryseus。这两个种分别采自于广东省的南岭自然保护区和海南省的尖峰岭自然保护区。根据所采集的材料对这两种真菌进行了详细描述。     

竹叶锈病重寄生现象及重寄生菌鉴定

竹叶锈病是竹类主要病害之一,影响竹林生长。为了利用重寄生菌对竹叶锈病进行生物防治,研究了竹叶锈病菌重寄生现象及重寄生菌的形态与分类地位。结果表明,该重寄生现象伴随竹叶锈病的发生而从4月下旬至5月初开始出现;重寄生菌的自然重寄生率于5月至8月初呈快速增长趋势,8月中旬达到高峰期,随后其重寄生率呈缓慢下降趋势;重寄生菌寄生在竹叶锈病夏孢子堆上呈白色的点状霉状物,阻碍了竹叶锈病夏孢子的发育和释放。通过对其分生孢子梗、分生孢子及菌丝等形态特征观察,该重寄生菌被鉴定为赭红枝顶孢Acremonium salmoneu     

Dissociation of expression patterns of homeodomain transcription factors in the evolution of developmental mode in the sea urchins Heliocidaris tuberculata and H. erythrogramma

The direct-developing sea urchin species Heliocidaris erythrogramma has a radically modified ontogeny. Along with gains of novel features, its entire ectoderm has been reorganized, resulting in the apparent absence of a differentiated oral ectoderm, a major module present in the pluteus of indirect-developing species, such as H. tuberculata. The restoration of an obvious oral ectoderm in H. erythrogrammaxH. tuberculata hybrids, indicates the action of dominant regulatory factors from the H. tuberculata genome. We sought candidate regulatory genes based on the prediction that they should include genes that govern development of the oral ectoderm in the pluteus, but play different roles in H. erythrogramma. Such genes may have a large effect in the evolution of development. Goosecoid (Gsc), Msx, and the sea urchin Abd-B-like gene (Hox11/13b) are present and expressed in both species and the hybrid embryos. Both Gsc and Msx are oral ectoderm specific in H. tuberculata, and show novel and distinct expression patterns in H. erythrogramma. Gsc assumes a novel ectodermal pattern and Msx shifts to a novel and largely mesodermal pattern. Both Gsc and Msx show a restoration of oral ectoderm expression in hybrids. Hox11/13b is not expressed in oral ectoderm in H. tuberculata, but is conserved in posterior spatial expression among H. tuberculata, H. erythrogramma and hybrids, serving as a control. Competitive RT-PCR shows that Gsc, Msx, and Hox11/13b are under different quantitative and temporal controls in the Heliocidaris species and the hybrids. The implications for the involvement of these genes in the rapid evolution of a direct developing larva are discussed.     

The molluscicidal activity of niclosamide (Bayluscide WP70(R)) on Melanoides tuberculata (Thiaridae), a snail associated with habitats of Biomphalaria glabrata (Planorbidae)

The aim of this study was to determine the toxicity of niclosamide (Bayluscide (R)) on Melanoides tuberculata and Biomphalaria glabrata under laboratory conditions. The latter species is the intermediate host of Schistosoma mansoni (Sambon 1917). M. tuberculata was successfully used as competitor of B. glabrata in biological control programs in French West Indies. Both molluscicide and biological control using M. tuberculata have proved to be successful in reducing the population density of B. glabrata. The associated use of molluscicide in this area would be an effective measure if M. tuberculata were less susceptibility to the molluscicide than B. glabrata. Three hundreds individuals each of B. glabrata and of M. tuberculata, collected in Sumidouro, State of Rio de Janeiro, were used in the experiment. The molluscs were exposed to 14 different concentrations of niclosamide as recommended by the World Health Organization. Probit analysis was used to determine the LC 50 and LC 90. The LC 50 and LC 90 values for B. glabrata were 0.077 mg/l and 0.175 mg/l, respectively and the LC 50 and LC 90 values for M. tuberculata were 0.082 mg/l and 0.221 mg/l respectively. As the lethal concentrations of niclosamide were approximately the same to both species, this could be a disadvantage when controlling B. glabrata with niclosamide in an area of M. tuberculata occurrence. It might therefore be preferable to utilize the latex extracted from the Euphorbia splendens, which presented a much higher efficiency for B. glabrata than to M. tuberculata.     

Interaction between the intermediate host of Schistosomiasis in Brazil Biomphalaria glabrata (Planorbidae) and a possible competitor Melanoides tuberculata (Thiaridae): I. Laboratory experiments

The biological control of Biomphalaria glabrata, intermediate host of Schistosoma mansoni, is one the accepted options to fight schistosomiasis. One of the most promising candidates to control B. glabrata is the snail Melanoides tuberculata, a potential competitor. However, the mechanisms of interaction between the two species are not clear. Our objective is to determine if M. tuberculata indeed compete with B. glabrata, using two laboratory experiments. In Experiment 1, we tested the effect of the presence of M. tuberculata on the fecundity and mortality rates of B. glabrata. In Experiment 2, we tested if there was a direct or indirect interaction between the two species. In Experiment 1, M. tuberculata was eliminated after the peak in reproductive activity of B. glabrata. In Experiment 2, B. glabrata produced more egg masses when raised with M. tuberculata. The conditions leading to this unexpected positive effect of M. tuberculata on the fecundity of B. glabrata need further clarification, but emphasize that detailed studies of the interaction between these species in the conditions of the local environment should be considered.     

Gene expression patterns in a novel animal appendage: the sea urchin pluteus arm

The larval arms of echinoid plutei are used for locomotion and feeding. They are composed of internal calcite skeletal rods covered by an ectoderm layer bearing a ciliary band. Skeletogenesis includes an autonomous molecular differentiation program in primary mesenchyme cells (PMCs), initiated when PMCs leave the vegetal plate for the blastocoel, and a patterning of the differentiated skeletal units that requires molecular cues from the overlaying ectoderm. The arms represent a larval feature that arose in the echinoid lineage during the Paleozoic and offers a subject for the study of gene co-option in the evolution of novel larval features. We isolated new molecular markers in two closely related but differently developing species, Heliocidaris tuberculata and Heliocidaris erythrogramma. We report the expression of a larval arm-associated ectoderm gene tetraspanin, as well as two new PMC markers, advillin and carbonic anhydrase. Tetraspanin localizes to the animal half of blastula stage H. tuberculata and then undergoes a restriction into the putative oral ectoderm and future location of the postoral arms, where it continues to be expressed at the leading edge of both the postoral and anterolateral arms. In H. erythrogramma, its expression initiates in the animal half of blastulae and expands over the entire ectoderm from gastrulation onward. Advillin and carbonic anhydrase are upregulated in the PMCs postgastrulation and localized to the leading edge of the growing larval arms of H. tuberculata but do not exhibit coordinated expression in H. erythrogramma larvae. The tight spatiotemporal regulation of these genes in H. tuberculata along with other ontogenetic and phylogenetic evidence suggest that pluteus arms are novel larval organs, distinguishable from the processes of skeletogenesis per se. The dissociation of expression control in H. erythrogramma suggest that coordinate gene expression in H. tuberculata evolved as part of the evolution of pluteus arms, and is not required for larval or adult development.     

The "lecithotrophic" sea urchin Heliocidaris erythrogramma lacks typical yolk platelets and yolk glycoproteins

The sea urchin Heliocidaris tuberculata undergoes typical development, forming an echinoid pluteus larva, whereas H. erythrogramma undergoes direct development via a highly modified, nonfeeding larva. Using a polyclonal antibody prepared against yolk glycoproteins from the typical developer Stronglyocentrotus purpuratus, we found that H. tuberculata contains cross-reactive proteins in abundance, but H. erythrogramma does not. In addition, we used immunoelectron microscopy to demonstrate that unfertilized eggs of H. tuberculata contain yolk platelets, but those of H. erythrogramma do not.     

Co-option and dissociation in larval origins and evolution: the sea urchin larval gut

SUMMARY The origin of marine invertebrate larvae has been an area of controversy in developmental evolution for over a century. Here, we address the question of whether a pelagic "larval" or benthic "adult" morphology originated first in metazoan lineages by testing the hypothesis that particular gene co-option patterns will be associated with the origin of feeding, indirect developing larval forms. Empirical evidence bearing on this hypothesis is derivable from gene expression studies of the sea urchin larval gut of two closely related but differently developing congenerics, Heliocidaris tuberculata (feeding indirect-developing larva) and H. erythrogramma (nonfeeding direct developer), given two subsidiary hypotheses. (1) If larval gut gene expression in H. tuberculata was co-opted from an ancestral adult expression pattern, then the gut expression pattern will remain in adult H. erythrogramma despite its direct development. (2) Genes expressed in the larval gut of H. tuberculata will not have a coordinated expression pattern in H. erythrogramma larvae due to loss of a functional gut. Five structural genes expressed in the invaginating archenteron of H. tuberculata during gastrulation exhibit substantially different expression patterns in H. erythrogramma with only one remaining endoderm specific. Expression of these genes in the adult of H. erythrogramma and larval gut of H. tuberculata , but not in H. erythrogramma larval endoderm, supports the hypothesis that they first played roles in the formation of adult structures and were subsequently recruited into larval ontogeny during the origin and evolution of feeding planktotrophic deuterostome larvae.     

Single-copy DNA distance between two congeneric sea urchin species exhibiting radically different modes of development

We have investigated the differences between nuclear genomes of two purportedly congeneric species of sea urchin that differ radically in early development. Heliocidaris tuberculata develops by means of a typical pluteus larva, whereas H. erythrogramma develops directly from an egg that is 100-fold the volume of the H. tuberculata egg. Reassociation kinetic analysis shows that the kinetic components of the genomic DNA from the two species are essentially the same. No single repeat component explains the 30% difference between the H. erythrogramma and H. tuberculata genomes. Reciprocal hybridization of tracer-labeled single-copy DNA fractions between these species indicates that approximately 50% of the single-copy DNA is sufficiently similar to form hybrids at standard hybridization criterion. Thermal denaturation profiles of the hybridized single-copy DNA sequence yields median (T50H) values of 13.8 degrees-16.5 degrees C. This result suggests a divergence time of 10-13 Mya, which is comparable to divergence times between congeneric sea urchin species in other genera that do not differ significantly in development. Radical differences in early developmental processes can evolve rapidly between closely related forms.     

Sequence Insertions and ITS Data Provide Congruent Information on Roccella canariensis and R. tuberculata (Arthoniales, Euascomycetes) Phylogeny

Four Roccella species, R. canariensis, R. fimbriata, R. montagnei, and R. tuberculata, were found to possess sequence insertions in up to four locations in the first half of the SSU rDNA. Insertions from one of these positions have been classified as group I introns, while the others may represent degenerative forms of group I introns or messenger RNA introns. Two of the insertion-containing taxa, R. canariensis and R. tuberculata, differ only in their dispersal strategy: R. canariensis is sexual, producing only fruiting bodies and R. tuberculata is sterile, producing only vegetative propagules, i.e., soredia. Because insertions occurred in specimens of both taxa, they were used to examine the phylogenetic relationships between and within the two species. The sequence insertions from each of the four positions were aligned and cladistically analyzed separately. Internal transcribed spacers (ITS) were additionally sequenced to study the phylogeny of all R. canariensis and R. tuberculata specimens. Three other Roccella species (R. babingtonii, R. fimbriata, and R. montagnei) and Dirina catalinariae were used as outgroups in this parsimony analysis. Sequence insertions were found to be potentially useful in phylogenetic studies, although due to the sequence dissimilarity, homology relations were difficult to establish above the species level and in some cases even within the species. The phylogenies obtained from the insertion matrices were totally consistent with the ITS data and the insertions were concluded to have been inherited. When the insertion and ITS data were combined for total evidence, R. canariensis and R. tuberculata did not form distinct lineages in the phylogenetic tree, but appeared mixed in well-supported groups containing both sorediate and fertile specimens.