广西恩城保护区黑叶猴种群数量和保护现状

黑叶猴是栖息于喀斯特石山地区的珍稀濒危灵长类动物,由于非法捕杀和栖息地丧失,种群数量急剧减少。2017年10-12月,我们采用样方法调查恩城国家级自然保护区及其周边地区黑叶猴的种群分布、数量及保护现状,分析其致危因素,为制定有效管理行动计划提供理论依据。本次调查共记录到黑叶猴夜宿地89处,其中有43处夜宿地下方有新鲜排泄物。结合本次调查和访问,确定黑叶猴的种群数量为18群100~105只,包括1只全白化黑叶猴成年雌性个体;其中11群分布在保护区内,7群分布在保护区外。猴群由3~12只个体组成。栖息地破碎化和退化是威胁恩城保护区黑叶猴种群生存的主要因素。     

广西崇左白头叶猴国家级自然保护区及周边区域黑叶猴种群数量与分布

2012年10~12月采用访问法、小区蹲点数量统计方法对广西崇左白头叶猴国家级自然保护区驮逐片及周边相邻的昌平片的黑叶猴种群数量与分布进行了调查。结果显示,驮逐片黑叶猴分布面积为9714 hm2,种群数量为10群55只;昌平片黑叶猴分布面积为1073 hm2,种群数量为7群33只;两处合计为17群88只。驮逐片和昌平片黑叶猴种群数量分别占广西黑叶猴种群数量的14%和10%。驮逐片通过加强能力建设、宣传力度和保护力度,昌平片通过建立自然保护小区,加大对黑叶猴及其栖息地的保护力度。     

贵州宽阔水国家级自然保护区黑叶猴种群数量与分布

2015年5—12月,基于宽阔水国家级自然保护区的黑叶猴历史资料设定重点调查区域,采用定点观察与痕迹观察资料收集以及非诱导式访问调查等方法对保护区黑叶猴种群数量和分布进行调查,并结合保护区巡护记录,重新确定黑叶猴在保护区的种群数量和分布区域。结果显示:保护区黑叶猴种群数量约为29群195只,比1995年增长了143.75%,比2003年增长了39.29%;主要分布区域位于保护区西南部的马蹄溪、白石溪、猪钻子沟、苏家沟、油筒溪、观音岩、底水、枇杷岩、让水坝等9个沟段中。全保护区内黑叶猴群体密度为0.11群/km~2,种群密度为0.74只/km~2,其中主要活动范围约32.50 km~2,主要活动范围内群体密度为0.89群/km~2,种群密度为6.00只/km~2。种群数量的稳定增长得益于保护区采取的一系列保护管理措施,有效制止了盗猎行为的发生,减少了附近居民活动对黑叶猴的干扰和影响。为进一步保护黑叶猴种群及其栖息地,建议保护区通过划定重点管护区域、加强科学管理、开展生境管理和调节、加强环境保护教育和社区共管等措施建立、健全保护管理机制。     

广西弄岗自然保护区黑叶猴的种群动态

2006年5～7月和2008年10～11月,采用访问和小区蹲点绝对数量统计法对弄岗自然保护区黑叶猴种群数量进行调查.结果 表明,弄岗黑叶猴分布区约为26 km2,2008年有黑叶猴13群76只,平均群大小5.85只/群,群密度0.5群/km2,种群密度为2.92只/km2.与2006年相比,2008年猴群增加3个两性群,个体数量增加10只,即增加15.15%,种群密度增加14.96%,平均群大小增加6.36%.弄岗黑叶猴种群数量呈现出增长趋势,这可能与保护区加强管护和宣传有关.     

黑叶猴食物组成的地域性差异比较

研究同一个物种在不同生境觅食对策的差异不仅有助于了解其行为和生态的可塑性,还有助于评价其对自然或人为干扰导致的生境质量的改变的适应能力,从而为物种的有效保护和管理提供科学依据。本文以广西弄岗国家级自然保护区和扶绥保护区的黑叶猴为研究对象,采用瞬时扫描取样法收集相关的食性数据,并结合贵州麻阳河国家级自然保护区黑叶猴的食性研究结果,比较分析黑叶猴食物组成的区域性差异以探讨黑叶猴对不同喀斯特石山生境的行为适应对策。结果表明:黑叶猴3个地理种群共采食77科、182属、259种植物,木本植物(包括乔木和灌木)是其最重要的食物来源。3个地理种群均表现出较高程度的叶食性,树叶分别占其食物组成的52.7%、63.9%和79.7%。然而,3个地理种群的食物组成却存在显著差异,种群间总的食物重叠指数仅为1.33%-7.43%,主要食物的重叠指数仅为0-13.51%。这些差异可能与栖息地中植被组成的差异有关。与弄岗和麻阳河黑叶猴相比,扶绥黑叶猴采食更多的嫩叶和灌木。人为干扰对栖息地植被结构的影响可能是导致上述差异的主要原因。我们认为黑叶猴不同地理种群食物组成的显著差异反映其觅食的灵活性以应对不同喀斯特石山生境。     

贵州典型自然保护区内外外来入侵草本植物的比较

自然保护区是抵御外来物种入侵的重要屏障, 然而人类活动可能增加外来入侵物种扩散的风险, 导致自然保护区面临不同程度的入侵。为了对比研究自然保护区内外以及不同保护区之间外来入侵草本植物的分布格局及其与人类活动的关系, 本文基于贵州省四个国家级自然保护区(赤水桫椤、习水、梵净山和麻阳河) 380个样方的调查数据, 运用双因素方差分析和非度量多维标度分析方法分析了保护区内外的外来入侵草本植物多样性和物种组成差异, 并利用冗余分析探讨了影响不同保护区内外来入侵草本植物分布的关键人类活动因子。赤水桫椤、习水、梵净山和麻阳河4个保护区共调查发现外来入侵草本植物46种, 隶属于18科37属, 其中, 4个保护区内分别有16、18、7和20种, 保护区外分别有17、10、24和20种。赤水桫椤和梵净山保护区外的外来入侵草本植物丰富度、Shannon-Wiener多样性指数和Simpson优势度指数显著高于保护区内, 而习水和麻阳河保护区内外差异不显著。梵净山保护区内的Pielou均匀度指数显著高于保护区外, 其余保护区内外差异不显著。四个保护区内外的外来入侵草本植物物种组成无明显差异。保护区内的居民量和道路长度是影响保护区内外来入侵草本植物分布格局差异的主要原因。研究结果表明, 不同保护区对外来草本植物入侵的抵御能力不同, 而人类活动程度的差异是导致其不同的原因之一。建议加强自然保护区内及周边区域人类活动管控, 减缓外来入侵植物的扩散。     

麻阳河国家级自然保护区两栖爬行动物资源调查

为进一步了解麻阳河国家级自然保护区两栖爬行类的资源分布和物种多样性特征,于2013年5~6月对麻阳河国家级自然保护区两栖爬行动物资源进行了实地考察。调查结果显示,麻阳河国家级自然保护区有两栖爬行动物43种,隶属4目14科34属。运用相似性指数对麻阳河、佛顶山、雷公山三个自然保护区两栖爬行动物进行比较,印证了动物地理区划上雷公山和佛顶山属于黔东南低山丘陵盆地省,麻阳河属于黔北中山峡谷省的划分。运用G-F指数公式分别计算三个自然保护区两栖动物和爬行动物G-F指数,其中,雷公山两栖动物和爬行动物G-F指数最高,分别为0.23和0.50,麻阳河两栖动物和爬行动物G-F指数最低,分别为﹣0.20和0.25,说明麻阳河国家级自然保护区中两栖动物和爬行动物的科间和科内多样性均较低。通过对三个自然保护区年均降水量、年均温等环境因子分析比较,得出麻阳河国家级自然保护区两栖爬行动物物种相对较少的原因,可能是麻阳河国家级自然保护区较低的年均降水量和较高的年均温。     

鄂西南国家级自然保护区群——苔藓植物多样性保护的重要场所

鄂西南地区密集分布有后河、木林子、七姊妹山和星斗山四大国家级自然保护区,共同形成了一个珍稀动植物大体相近、互相补充的保护区群,为摸清鄂西南保护区群的苔藓植物组成,该文采用野外调查和文献资料整理相结合的方法,对鄂西南国家级自然保护区群内的苔藓植物丰富度和组成特征进行了分析,并与渝东南、湘西北的苔藓植物多样性进行了比较。结果表明:(1)鄂西南国家级自然保护区群共有苔藓植物77科197属601种,物种数分别占中国和湖北苔藓总数的19.89%和71.46%。其中,中国特有种27种,仅含1种的科有15科,仅含1种的属有91属。(2)鄂西南国家级保护区群内的各保护区之间的苔藓物种存在一定的相似性与互补性,符合我国生物多样性保护原则。(3)鄂西南国家级自然保护区群苔藓植物区系类型全面,物种数量也显著高于同处武陵山区的渝东南、湘西北等地区。因此,鄂西南国家级自然保护区群不仅有效地保护了大型珍稀濒危动植物,而且还孕育了丰富多彩的苔藓植物类群为苔藓植物营造了良好的居住环境,是苔藓植物多样性保护的重要场所。     

贵州麻阳河地区野生黑叶猴与同域放养山羊冬季食物重叠的初步分析

于2009年12月～2010年2月采用瞬时扫描取样法对贵州麻阳河自然保护区的5个黑叶猴Trachypithecus francoisi猴群以及同域放养的山羊羊群的取食行为进行观察记录,并对两者的取食数据进行比较分析,以期了解该地区黑叶猴与放养山羊在食物资源最为匮乏的冬季的资源竞争关系,为黑叶猴的保护管理提供科学依据.研究结果表明:黑叶猴冬季取食植物67种,放养山羊同期取食植物44种,两者食物重叠26种;对26种重叠食物的采食分别占黑叶猴与放养山羊冬季取食时间的76.2％与22.0％;黑叶猴冬季采食的19种主要食物(＞1％冬季采食时间)中,有14种为放养山羊所采食,占黑叶猴与山羊冬季采食时间的71.7％与17.8％;黑叶猴与山羊冬季食物生态位宽度分别为0.68与0.39,两者的冬季食物生态位重叠指数C=0.74.表明贵州麻阳河地区黑叶猴与同域放养山羊在食物最为匮乏的冬季存在较为激烈的食物资源竞争关系.     

我国东北地区自然植被保护现状及保护优先区分析

以我国东北地区陆域国家级自然保护区、植被类型和海拔信息作为基础数据,采用叠加分析的方法,分析了自然保护区分布和植被分布与海拔的关系,以期对我国东北地区自然植被从水平空间和垂直空间两个维度上提出保护指导。结果表明:(1)森林生态系统、内陆湿地和野生动物类型的国家级自然保护区是我国东北地区国家级自然保护区的主体。随着海拔的升高,保护区的面积和数量都呈现下降趋势,在中海拔段(600～1000 m)出现了内陆湿地类型向森林生态系统类型自然保护区过渡的现象。(2)高海拔段(1000 m)的自然植被得到了较好的保护,但中、低海拔段(1000 m)的自然植被未受到较好的保护,仍然存在保护空缺。(3)一级保护优化区集中在长白山中部和东北部林区、辽河口、通辽东北部和呼伦贝尔高原,二级保护优先区分布在东北山区(大、小兴安岭和长白山地区),特别是大、小兴安岭交接地带,以及大兴安岭南段森林草原过渡区和辽东丘陵地区。(4)栽培作物作为保护区内植被的主要人为干扰来源,未得到足够重视,严重威胁到了东北平原地区内陆湿地类型和野生动物类型的国家级自然保护区。因此,建议在长白山中部林区的一级保护优化区内,设立以国家公园为主体的自然保护地体系进行保护,其余一级保护优化区可考虑扩大现有保护区或增设新的保护区;而低海拔段受栽培作物干扰严重的地区,应加强管理监督,对于破坏后需恢复的植被地带,可对其破坏后形成的次生天然植被进行保护,以实现区域内生物多样性的保护目标。     

Collagen-like triple helix formation of synthetic (Pro-Pro-Gly)10 analogues: (4(S)-hydroxyprolyl-4(R)-hydroxyprolyl-Gly)10, (4(R)-hydroxyprolyl-4(R)-hydroxyprolyl-Gly)10 and (4(S)-fluoroprolyl-4(R)-fluoroprolyl-Gly)10.

For the rational design of a stable collagen triple helix according to the conventional rule that the pyrrolidine puckerings of Pro, 4-hydroxyproline (Hyp) and 4-fluoroproline (fPro) should be down at the X-position and up at the Y-position in the X-Y-Gly repeated sequence for enhancing the triple helix propensities of collagen model peptides, a series of peptides were prepared in which X- and Y-positions were altogether occupied by Hyp(R), Hyp(S), fPro(R) or fPro(S). Contrary to our presumption that inducing the X-Y residues to adopt a down-up conformation would result in an increase in the thermal stability of peptides, the triple helices of (Hyp(S)-Hyp(R)-Gly)(10) and (fPro(S)-fPro(R)-Gly)(10) were less stable than those of (Pro-Hyp(R)-Gly)(10) and (Pro-fPro(R)-Gly)(10), respectively. As reported by B?chinger's and Zagari's groups, (Hyp(R)-Hyp(R)-Gly)(10) which could have an up-up conformation unfavorable for the triple helix, formed a triple helix that has a high thermal stability close to that of (Pro-Hyp(R)-Gly)(10). These results clearly show that the empirical rule based on the conformational preference of pyrrolidine ring at each of X and Y residues should not be regarded as still valid, at least for predicting the stability of collagen models in which both X and Y residues have electronegative groups at the 4-position.     

Synthesis and luminescence properties of a blue-emitting Sr(3.5) Y(6.5) O(2) (PO(4) )(1.5) (SiO(4) )(4.5) :Eu(2+) phosphor

A novel blue‐emitting Sr_3.5Y_6.5O₂(PO₄)_1.5(SiO₄)_4.5:Eu²⁺ phosphor was synthesized via a solid‐state reaction. Powder X‐ray diffraction (XRD) analysis demonstrated that the Sr_3.5Y_6.5O₂(PO₄)_1.5(SiO₄)_4.5 host had a hexagonal crystal structure in the space group P6₃/m and unit cell parameters a = 9.418 Å, c = 6.900 Å. The as‐prepared phosphor showed a blue emission and all the main emission peaks were located at around 466 nm for different excitation wavelengths of 297, 333 and 391 nm. The temperature dependence of the photoluminescence property was investigated in the range 20–250 °C, and the emission intensity decreased to 71% of the initial value at room temperature on increasing the temperature to 150 °C. According to the classical theory of fluorescent thermal quenching, the activation energy (ΔE) for the thermal quenching luminescence of the as‐prepared Sr_3.45Y_6.5O₂(PO₄)_1.5(SiO₄)_4.5:0.05Eu²⁺ phosphor was determined to be 0.20 eV. Copyright © 2011 John Wiley & Sons, Ltd.     

Role of tfdC(I)D(I)E(I)F(I) and tfdD(II)C(II)E(II)F(II) gene modules in catabolism of 3-chlorobenzoate by Ralstonia eutropha JMP134(pJP4)

The enzymes chlorocatechol-1,2-dioxygenase, chloromuconate cycloisomerase, dienelactone hydrolase, and maleylacetate reductase allow Ralstonia eutropha JMP134(pJP4) to degrade chlorocatechols formed during growth in 2,4-dichlorophenoxyacetate or 3-chlorobenzoate (3-CB). There are two gene modules located in plasmid pJP4, tfdC(I)D(I)E(I)F(I) (module I) and tfdD(II)C(II)E(II)F(II) (module II), putatively encoding these enzymes. To assess the role of both tfd modules in the degradation of chloroaromatics, each module was cloned into the medium-copy-number plasmid vector pBBR1MCS-2 under the control of the tfdR regulatory gene. These constructs were introduced into R. eutropha JMP222 (a JMP134 derivative lacking pJP4) and Pseudomonas putida KT2442, two strains able to transform 3-CB into chlorocatechols. Specific activities in cell extracts of chlorocatechol-1,2-dioxygenase (tfdC), chloromuconate cycloisomerase (tfdD), and dienelactone hydrolase (tfdE) were 2 to 50 times higher for microorganisms containing module I compared to those containing module II. In contrast, a significantly (50-fold) higher activity of maleylacetate reductase (tfdF) was observed in cell extracts of microorganisms containing module II compared to module I. The R. eutropha JMP222 derivative containing tfdR-tfdC(I)D(I)E(I)F(I) grew four times faster in liquid cultures with 3-CB as a sole carbon and energy source than in cultures containing tfdR-tfdD(II)C(II)E(II)F(II). In the case of P. putida KT2442, only the derivative containing module I was able to grow in liquid cultures of 3-CB. These results indicate that efficient degradation of 3-CB by R. eutropha JMP134(pJP4) requires the two tfd modules such that TfdCDE is likely supplied primarily by module I, while TfdF is likely supplied by module II.     

Induction of in vitro flowering in Dendrobium Madame Thong-In (Orchidaceae) seedlings is associated with increase in endogenous N(6)-(Delta (2)-isopentenyl)-adenine (iP) and N (6)-(Delta (2)-isopentenyl)-adenosine (iPA) levels

We analysed the endogenous cytokinin levels of Dendrobium Madame Thong-In seedlings grown in vitro during vegetative and flowering-inductive periods. HPLC was used to fractionate the extracts and radioimmunoassay (RIA) was used for assay of zeatin (Z), dihydrozeatin (DZ), N(6)-(Delta(2)-isopentenyl)-adenine (iP) and their derivatives. Coconut water used in experiments was found to contain high level (>136 pmol ml(-1)) of zeatin riboside (ZR). Protocorms and seedlings cultured in medium with coconut water were found to contain 0.5-3.9 pmol g(-1) FW of the cytokinins analysed. Seedlings (1.0-1.5 cm) cultured in flowering-inductive liquid medium containing 6-benzyladenine (BA, 4.4 muM) and coconut water (CW, 15%) contained up to 200 and 133 pmol g(-1) FW of iP and iPA, respectively. These levels were significantly higher than all other cytokinins analysed in seedlings of the same stage and were about 80- to 150-folds higher than seedlings cultured in non-inductive medium. During the transitional (vegetative to reproductive) stage, the endogenous levels of iP (178 pmol g(-1) FW) and iPA (63 pmol g(-1) FW) were also significantly higher than cytokinins in the zeatine (Z) and dihydrozeatin (DZ) families in the same seedlings. Seedlings that grew on inductive medium but remained vegetative contained lower levels of iPA. The importance of the profiles of iP and its derivatives in induction of in vitro flowering of D. Madame Thong-In is discussed.     

Biology of Bactrocera (Zeugodacus) tau (Walker) (Diptera: Tephritidae)

The biology of the fruit fly Bactrocera tau, an important horticultural pest, was studied under laboratory conditions at 25°C and 60–70% relative humidity on Cucurbita maxima. The duration of mating averaged 408.03 ± 235.93 min. After mating, the female fly had a preoviposition period of 11.7 ± 4.49 days. The oviposition rate was 9.9 ± 8.50 eggs and fecundity was 464.6 ± 67.98 eggs/female. Eggs were elliptical, smooth and shiny white, turning darker as hatching approached, and measured 1.30 ± 0.07 mm × 0.24 ± 0.04 mm. The chorion has polygonal microsculpturing and is species-specific with polygonal walls. The egg period lasts for 1.3 ± 0.41 days. The duration of the larval period is 1.2 ± 0.42, 1.7 ± 0.48 and 4.0 ± 0.94 days for first, second and third instars, respectively. Pupation occurs in the sand or soil and pupal periods are 7.0 ± 0.47 days. The life cycle from egg to adult was completed in 14.2 ± 1.69 days; the longevity of mated females and males was 130.33 ± 14.18 and 104.66 ± 31.21 days, respectively. At least two to three generations were observed from June 2008 to June 2009.     

The helix-coil transitions of poly (dA)-poly (dT) and poly (dA-dT)-poly (dA-dT)

Helix–coil transition curves are calculated for poly (dA) poly(dT) and poly (dA-dT) poly (dA-dT) using the integral equation approach of Goel and Montroll.⁵ The transitions are described by the loop entropy model with the exponent of the loop entropy factor, k, remaining an arbitrary constant. The theoretical calculations are compared with experimental transition curves of the two polymers. Results indicate that the stacking energies for these two polymers differ by about 1 kcal/mole of base pairs. Also, a fit between theory and experiment was not possible for k > 1.70.     

Spectroscopic investigations of Er(3+) :CdO-Bi(2) O(3) -B(2) O(3) glasses

This article reports on the optical properties of Er³⁺ ions doped CdO–Bi₂O₃–B₂O₃ (CdBiB) glasses. The materials were characterized by optical absorption and emission spectra. By using Judd–Ofelt theory, the intensity parameters Ω_λ (λ = 2, 4, 6) and also oscillatory strengths were calculated from the absorption spectra. The results were used to compute the radiative properties of Er³⁺:CdBiB glasses. The concentration quenching and energy transfer from Yb³⁺–Er³⁺ were explained. The stimulated emission cross‐section, full width at half maximum (FWHM) and FWHM × values are also calculated for all the Er³⁺:CdBiB glasses. Copyright © 2011 John Wiley & Sons, Ltd.     

Cationic (tripyrrinato)palladium(II) complexes

The formation of cationic palladium(II)complexes [TrpyPd^II]⁺X⁻ by salt metathesis of the respective trifluoroacetates with different salts of weakly coordinating anions X⁻ was investigated. With non-hydrolizable counterions, cationic mono- and dinuclear complexes are observed depending on the nature of the anion X⁻ and the solvent. The mononuclear cations, which are only formed with X = BAr^F, most probably carry a weakly bound molecule of dichloromethane at the fourth coordination site of Pd^II. When treated with diazoalkanes, only these are sufficiently reactive to form carbene complexes. Four- and five coordinate Lewis base adducts [TrpyPd^IIL]⁺ with L = CH₃NC, tBuNH₂, PMe₃, PEt₃ and PiPr₃ and [TrpyPd^IIL₂]⁺ with L = PMe₃ were prepared from the mononuclear cations [TrpyPd^II]⁺BAr^F−. From structural studies it becomes apparent, that the formation of stable five coordinate Pd^II species is restricted to medium size ligands and depends on the delicate balance between the steric influence of L and the strain, which is induced on the TrpyPd^II unit.     

Copper (II) and cobalt (II) complexes of chiral tetrathiafulvalene-oxazoline (TTF-OX) and tetrathiafulvalene-thiomethyl-oxazoline (TTF-SMe-OX) derivatives

Synthesis and single crystal X-ray structures of the first paramagnetic transition metal complexes containing chiral ethylenedithio-tetrathiafulvalene-oxazoline (EDT-TTF-OX) 1a-c and ethylenedithio-tetrathiafulvalene-thiomethyloxazoline 2 (EDT-TTF-(SMe)OX) ligands based on copper (II) and cobalt (II) are described. The racemic [EDT-TTF-OX][Cu(hfac)₂] complex 3a crystallizes in the triclinic centrosymmetric space group , whereas the enantiopure counterparts 3b-c crystallize in the triclinic non-centrosymmetric space group P1. Cu(II) adopts a distorted square pyramidal coordination geometry, a much weaker Cu?S_TTF interaction also being identified. The same coordination pattern around Cu(II) is observed in the complex [(rac)-EDT-TTF-(SMe)OX][Cu(hfac)₂] (4) in spite of the bidentate nature of the redox active ligand. DFT theoretical calculations afforded two equilibrium configurations for a corresponding model complex, in which the metal centre establishes secondary coordination either with one S_TTF or with the SMe group. The same ligand coordinates the cobalt (II) to afford the octahedral complex [(rac)-EDT-TTF-(SMe)OX][Co(hfac)₂] (5). In all these novel complexes, the paramagnetic centres are structurally and magnetically isolated. Cyclic voltammetry measurements show the stability of the radical cation species.