中国蚁粉蚧属的初步研究（同翅目：蚧总科：粉蚧科）

记述我国蚁粉蚧属一新种：杨凌蚁粉蚧Formicococcus yanglingensis sp.nov.,建立一新组合;天麻蚁粉蚧F.gasteris(Wang,1982)comb.nov.(移自簇粉蚧属Paraputo Liang),并将F.gastrodiae Tang(1992)作为该种的异名处理。此外,还编制了蚁粉蚧属中国种类分种检索表。新种模式标本保存在南开大学生物系昆虫标本室。     

中国拂粉蚧属一新记录种（半翅目，蚧总科，粉蚧科）

热带拂粉蚧Ferrisia malvastra(McDaniel),是一种分布广泛的多食性害虫,可取食30科58种植物,分布29个国家和地区.2014年6月于云南省大理市一未知植物上发现该虫,这是该虫在我国的首次报道.本文重新描述了热带拂粉蚧雌成虫形态特征并附特征图,并介绍其寄主植物、地理分布、生物学及经济意义.     

中国粉蚧属一新纪录种(半翅目,蚧总科,粉蚧科)

菲律宾粉蚧Pseudococcus philippinicus Williams,2004为中国新纪录种。重描了雌成虫的形态特征,首次记述了各龄若虫的形态特征,绘制了各龄的形态特征图,拍摄了各虫龄的外形照片。最后,还编制了粉蚧属中国已知种类检索表。     

危害玉蜀黍的葵粉蚧属新种记述(同翅目:蚧总科, 粉蚧科)

本文记述葵粉蚧属一新种,取名为耕葵粉蚧。新种的正模和副模标本均保存在中国科学院动物研究所。 该粉蚧发现于七十年代初,对此蚧虫的经济意义有一个初步认识过程。最早只知其寄生在高粱的根部。后来在玉蜀黍的根部,有时也在玉蜀黍近地面的叶鞘内发现寄生着密集的虫体。被寄生的植株,轻者生长发育缓慢,矮小细弱,减少产量;重者难以结实,甚至枯萎而死。该蚧虫或造成局部地区的玉蜀黍农田受害日趋明显,或在其分布区内大发生,此起彼伏,难以掌握,所以旧稿数易。根据这一害虫的发展情况应引起有关部门的关注,尽快进行对该蚧虫的观察研究工作,以便及时采取控制其猖獗的措施,确保粮食丰产。现将新种记述如下。     

安徽泡粉蚧属一新种(同翅目:蚧总科:粉蚧科)

记述采自安徽黄山的泡粉蚧属一新种,即黄山泡粉蚧Puto huangshanensis sp.nov.,模式标本保存在北京林业大学昆虫标本室。     

中国绵粉蚧属种类记述(同翅目:蚧总科:粉蚧科)

研究和整理了中国绵粉蚧属Ｐｈｅｎａｃｏｃｃｕｓ Ｃｏｃｋｅｒｅｌｌ的种类和分布。截至目前,我国该属计有２０种,其中包括本文３新种：无管绵粉蚧Ｐ．ａｔｕｂｕｌａｔｕｓ ｓｐ．ｎｏｖ．,内蒙粉蚧Ｐ．ｎｅｉｍｅｎｇｕｌｉｃｕｓ ｓｐ．ｎｏｖ．和山西绵粉蚧Ｐ．ｓｈａｎｘｉｅｎｓｉｓ ｓｐ．ｎｏｖ．及６个中国新纪录种：盐木绵粉蚧Ｐ．ａｒｔｈｒｏｐｈｙｔｉ Ａｒｃｈａｎｇｅｌｓｋａｙａ,时麦绵粉蚧Ｐ．ｐｒｅｉ     

云南根粉蚧属一新种记述(半翅目,粉蚧科,根粉蚧亚科)

报道采自云南勐腊的根粉蚧属1新种：三裂根粉蚧Rhiwecus triarculus sp.nov.。新种在形态上与日本种Rh．avenoides Takagi ＆Kawai,1971相近,但新种腹脐3个,且缺管腺。模式标本保存在北京林业大学森林保护系昆虫标本室。     

中国竹类粉蚧一新属一新种(半翅目,蚧总科,粉蚧科)

记述粉蚧科1新属1新种,即拟囊粉蚧属Paraporisaccus gen.nov.及其模式种贵州拟囊粉蚧P.guizhouensis sp.nov.。新种模式标本采自贵州省绥阳县宽阔水自然保护区1种玉山竹Yushania sp.小杆叶鞘下茎上,模式标本保存于北京林业大学昆虫标本室。     

中国星粉蚧属一新纪录种（半翅目，蚧总科，粉蚧科）

群管星粉蚧Heliococcus dorsiporosus Danzig为中国新纪录种。本文重描了雌成虫的形态特征,首次记述了一龄若虫的形态特征,绘制了形态特征图。此外,还编制了星粉蚧属中国已知种类检索表。     

云南土粉蚧属一新种记述（半翅目：粉蚧科：根粉蚧亚科）

记述采自云南施甸烤烟根部的土粉蚧属Ripersiella 1新种,即烤烟土粉蚧R.nicotiana,sp.nov.,并与近似种R.sabahica(Williams,2004)做了比较。模式标本保存在北京林业大学昆虫标本室。     

Effects of prostaglandins, dibutyryl cAMP, LHRH, estrogens, progesterone, and potassium on output of prostaglandin F2 alpha, 13, 14-dihydro-15-keto-prostaglandin F2 alpha, hCG, estradiol, and progesterone by placental minces

In order to compare the endocrine response of placental minces to luteinizing hormone releasing hormone (LHRH) and dibutyryl cAMP (dbcAMP) and to screen for effects of potential stimulatory and inhibitory substances, the simultaneous outputs of PGF2 alpha, 13, 14-dihydro-15-keto-prostaglandin F2 alpha (PGFM), progesterone, 17 beta-estradiol, and hCG were evaluated during a 4 hour incubation in 5 placentas. The output of hCG was highest for 12-week placentas, intermediate for a 16 week placenta, and lowest for term placentas. The output of 17 beta-estradiol by 12 and 16 week placentas in the presence of 30 microM dehydroepian-drosterone sulfate (DHEAS) was greater than that by term placentas. Progesterone output was apparently independent of gestational age although some variation between 12-week placentas was demonstrated. Output of PGF2 alpha was lower in 12 and 16-week placentas than in term placentas and that of PGFM was lower in 12-week placentas than in term placentas. LHRH (100 nM) produced stimulation of PGF2 alpha output (P less than .005) and a trend toward inhibition of progesterone output (which failed to achieve statistical significance) but no stimulation of hCG under these conditions. Stimulation of the outputs of hCG (P less than .005) and PGF2 alpha (P less than .001) and inhibition of that of progesterone (P less than .005) was produced by 20 mM dbcAMP. DHEAS inhibited output of progesterone (P less than .01) and PGF2 alpha (P less than .01). There were no effects of potassium, estrogens, progesterone, or prostaglandins on output of any measured substance.     

Simultaneous Bio-reduction of Nitrate,Perchlorate, Selenate,Chromate, Arsenate,and Dibromochloropropane Using a Hydrogen-based Membrane Biofilm Reactor

We tested the hypothesis that the H₂-based membrane biofilm reactor (MBfR) is capable of reducing multiple oxidized contaminants, a common situation for groundwater contamination. We conducted bench-scale experiments with three groundwater samples collected from California’s San Joaquin Valley and on two synthetic groundwaters containing selenate and chromate. The actual groundwater sources had nitrate levels exceeding 10 mg-N l⁻¹ and different combinations of anthropogenic perchlorate + chlorate, arsenate, and dibromochloropropane (DBCP). For all actual groundwaters, the MBfR reduced nitrate to less than 0.01 mg-N l⁻¹. Present in two groundwaters, perchlorate + chlorate was reduced to below the California Notification Level, 6 μg-ClO₄ l⁻¹. As(V) was substantially reduced to As(III) for two groundwaters samples, which had influent As(V) concentrations from 3 to 8.8 μg-As l⁻¹. DBCP, present in one groundwater at 1.4 μg l⁻¹, was reduced to below its detection limit of 0.01 μg l⁻¹, which is well below California’s 0.2 μg l⁻¹ MCL for DBCP. For the synthetic groundwaters, two MBfRs initially reduced Se(VI) or Cr(VI) stably to Se° or Cr(III). When we switched the influent oxidized contaminants, the new oxidized contaminant was reduced immediately, and its reduction soon was approximately the same or greater than it had been reduced in its original MBfR. These results support that the H₂-based MBfR can reduce multiple oxidized contaminants simultaneously.     

Bioavailability of selenium from veal, chicken, beef, pork, lamb, flounder, tuna, selenomethionine, and sodium selenite assessed in selenium-deficient rats

The bioavailability of selenium (Se) from veal, chicken, beef, pork, lamb, flounder, tuna, selenomethionine (SeMet), and sodium selenite was assessed in Se-deficient Fischer-344 rats. Se as veal, chicken, beef, pork, lamb, flounder, tuna, SeMet, and sodium selenite was added to torula yeast (TY) basal diets to comprise Se-inadequate (0.05 mg Se/kg) diets. Se as sodium selenite was added to a TY basal diet to comprise a Se-adequate (0.10 mg Se/kg), Se-control diet. The experimental diets were fed to weanling Fischer-344 rats that had been subjected to dietary Se depletion for 6 wk. After 9 wk of the dietary Se repletion, relative activity of liver glutathione peroxidase (GSHPx) from the different dietary groups compared with control rats (100%) was: flounder 106%, tuna 101%, pork 86%, sodium selenite 81%, SeMet 80%, beef 80%, chicken 77%, veal 77%, and lamb 58%. Se from flounder was the most efficient at restoring Se concentrations in the liver and skeletal muscle. Se from sodium selenite, SeMet, beef, veal, chicken, pork, lamb, and tuna was not dietarily sufficient to restore liver and muscle Se after 9 wk of recovery following a 6-wk period of Se depletion.     

Bioaccessibility of Cd,Cr, Cu,Mn, Ni,Pb, and Zn in Mediterranean mussel (Mytilus galloprovincialis Lamarck, 1819) along the southeastern Black Sea coast

The adverse effect of seafood consumption on human health is related to the bioaccessibility in contrast with the total heavy metal level in the tissues. In this study, bioaccessibility of Cd, Cr, Cu, Mn, Ni, Pb, and Zn in Mediterranean mussel (Mytilus galloprovincialis Lamarck, 1819) edible soft tissues collected along the southern Black Sea coast were investigated using in vitro digestion model. Total Pb concentration in the tissues was found to be higher than the maximum permissible limits set by European Commission. The bioaccessibility of metals in the tissues was found to be decreased in the order: Ni (83.11%) > Cu (80.47%) > Zn (76.86%) > Cd (73.32%) > Mn (69.11%) > Pb (61.07%) > Cr (58.44%). While significant positive linear regression was observed between total and bioaccessible concentrations for Mn, Pb, and Ni, significant negative linear regression was found for Cd (p < .05). The hazard quotients (HQ) calculated using bioaccessible amount were detected lower than the limit (HQ <1), which may not pose a potential hazard to humans reported by US Environmental Protection Agency. In sum, mussel consumption from sampling sites may cause a potential risk concerning human health especially for Cd and Pb in case of increased portion sizes.     

Determination of total aluminum, chromium, copper, iron, manganese, and nickel and their fractions leached to the infusions of black tea, green tea, Hibiscus sabdariffa, and Ilex paraguariensis (mate) by ETA-AAS

Total aluminum, chromium, copper, iron, manganese, and nickel were determined in black tea, green tea, Hibiscus sabdariffa, and Ilex paraguariensis (mate) by electrothermal atomic absorption spectrometry after nitric/perchloric acid digestion. In each case, one ground sample of commercially available leafy material was prepared and three 0.5-g subsamples were run in parallel. The infusions were also analyzed and the percentage of each element leached into the liquor was evaluated. The obtained results indicated that hibiscus and mate contained lower levels of aluminum (272±19 μg/g and 369±22 μg/g, respectively) as referred to black tea (759±31 μg/g) or green tea (919±29 μg/g) and suggested that mate drinking could be a good dietary source of essential micronutrient manganese (total content 2223±110 μg/g, 48.1% leached to the infusion). It was also found that the infusion of hibiscus could supply greater amounts of iron (111±5 μg/g total, 40.5% leached) and copper (5.9±0.3 μg/g total, 93.4% leached) as compared to other infusions. Moreover, it was found that the percentage of element leached to the infusion was strongly related to the tannins content in the beverage (correlation coefficients >0.82 with the exception for nickel); for lower tannins level, better leaching was observed.     

The effects of cryopreservation on the acrosome structure,enzyme activity,motility, and fertility of bovine,ovine, and goat sperm

The study was designed to investigate the effects of cryopreservation on bovine, ovine, and goat sperm motility, acrosome structure, enzyme activity, and fertilization ability. Percentage of sperm with hyaluronidase enzyme (HYD) activity was detected by a modified sodium hyaluronate-gelatin membrane. The N-α-benzoyl-DL-arginine-p-nitroanilide (BNPNA) method was used to assess the sperm acrosome enzyme (ACE). The mean percentage of sperm acrosome integrity dropped significantly (P < 0.01) after cryopreservation. The ACE activity of bovine sperm (100.48) was higher (P < 0.01) than that of ovine (57.88) or goat sperm (50.30), while the percentage of sperm with HYD activity of bovine (71.10%) and ovine (67.60%) sperm was higher than that of goat sperm (58.52%) after cryopreservation (P < 0.01). Sperm motility was positively correlated with the activity of the two acrosome enzymes before and after cryopreservation (P < 0.01). Cryopreservation had a negative effect on acrosomal morphology, motility, and acrosomal enzyme activity in their sperm. The fertilization ability of ovine and goat sperm decreased significantly after cryopreservation, but that of frozen bovine sperm did not differ significantly when compared with fresh sperm. There was no significant difference between ovine and goat sperm indices, except for percentage of sperm with HYD activity.     

CaMKII inhibition in heart failure, beneficial, harmful, or both

Calmodulin-dependent protein kinase II (CaMKII) has been proposed to be a therapeutic target for heart failure (HF). However, the cardiac effect of chronic CaMKII inhibition in HF has not been well understood. We have tested alterations of Ca(2+) handling, excitation-contraction coupling, and in vivo β-adrenergic regulation in pressure-overload HF mice with CaMKIIδ knockout (KO). HF was produced in wild-type (WT) and KO mice 1 wk after severe thoracic aortic banding (sTAB) with a continuous left ventricle (LV) dilation and reduction of ejection fraction for up to 3 wk postbanding. Cardiac hypertrophy was similar between WT HF and KO HF mice. However, KO HF mice manifested exacerbation of diastolic function and reduction in cardiac reserve to β-adrenergic stimulation. Compared with WT HF, L-type calcium channel current (I(Ca)) density in KO HF LV was decreased without changes in I(Ca) activation and inactivation kinetics, whereas I(Ca) recovery from inactivation was accelerated and Ca(2+)-dependent I(Ca) facilitation, a positive staircase blunted in WT HF, was recovered. However, I(Ca) response to isoproterenol was reduced. KO HF myocytes manifested dramatic decrease in sarcoplasmic reticulum (SR) Ca(2+) leak and slowed cytostolic Ca(2+) concentration decline. Sarcomere shortening was increased, but relaxation was slowed. In addition, an increase in myofilament sensitivity to Ca(2+) and the slow skeletal muscle troponin I-to-cardiac troponin I ratio and interstitial fibrosis and a decrease in Na/Ca exchange function and myocyte apoptosis were observed in KO HF LV. CaMKIIδ KO cannot suppress severe pressure-overload-induced HF. Although cellular contractility is improved, it reduces in vivo cardiac reserve to β-adrenergic regulation and deteriorates diastolic function. Our findings challenge the strategy of CaMKII inhibition in HF.     

Crystal structure, detonation performance, and thermal stability of a new polynitro cage compound: 2, 4, 6, 8, 10, 12, 13, 14, 15-nonanitro-2, 4, 6, 8, 10, 12, 13, 14, 15-nonaazaheptacyclo [5.5.1.13, 11. 15, 9] pentadecane

A new polynitro cage compound 2, 4, 6, 8, 10, 12, 13, 14, 15-nonanitro-2, 4, 6, 8, 10, 12, 13, 14, 15-nonaazaheptcyclo [5.5.1.1(3,11).1(5,9)] pentadecane (NNNAHP) was designed in the present work. Its molecular structure was optimized at the B3LYP/6-31 G(d,p) level of density functional theory (DFT) and crystal structure was predicted using the Compass and Dreiding force fields and refined by DFT GGA-RPBE method. The obtained crystal structure of NNNAHP belongs to the P-1 space group and the lattice parameters are a = 9.99 ?, b = 10.78 ?, c = 9.99 ?, α = 90.01°, β = 120.01°, γ = 90.00°, and Z = 2, respectively. Based on the optimized crystal structure, the band gap, density of state, thermodynamic properties, infrared spectrum, strain energy, detonation characteristics, and thermal stability were predicted. Calculation results show that NNNAHP has detonation properties close to those of CL-20 and is a high energy density compound with moderate stability.     

Stereoselective degradation of benalaxyl in tomato, tobacco, sugar beet, capsicum, and soil

The stereoselective degradation of the racemic benalaxyl in vegetables such as tomato, tobacco, sugar beet, capsicum, and the soil has been investigated. The two enantiomers of benalaxyl in the matrix were extracted by organic solvent and determined by validated chiral high-performance liquid chromatography with a cellulose-tris-(3, 5-dimethylphenylcarbamate)-based chiral column. Rac-benalaxyl was fortified into the soil and foliar applied to vegetables. The assay method was linear over a range of concentrations (0.5-50 microg ml(-1)) and the mean recoveries in all the samples were more than 70% for the two enantiomers. The limit of detection for both enantiomers was 0.05 microg g(-1). The results in soil showed that R-(-)-enantiomer dissipated faster than S-(+)-enantiomer and the stereoselectivity might be caused by microorganisms. In tomato, tobacco, sugar, beet, and capsicum plants, there was significantly stereoselective metabolism. The preferential absorption and degradation of S-(+)-enantiomer resulted an enrichment of the R-(-)-enantiomer residue in all the vegetables.     

Facile, alternative route to lubeluzole, its enantiomer, and the racemate

Lubeluzole [(S)-9] has been synthesized by a convergent synthesis, alkylation of N-methyl-N-piperidin-4-yl-1,3-benzothiazol-2-amine (4) with (+)-(R)-1-chloro-3-(3,4-difluorophenoxy)propan-2-ol [(+)-(R)-8] being the key step. Alcohol (+)-(R)-8 was obtained from commercially available (R)-epichlorohydrin [(R)-6], while the thiazole derivative 4 was easily obtained starting from N-protected piperidin-4-one (1) in a three-step procedure. The same method was used in order to obtain both the (R)-stereoisomer of lubeluzole [(R)-9] and its racemate [(RS)-9]. Overall yields ranged from 20% to 35%. The enantiomeric excess values for (S)-9 and (R)-9 were 97% and 94% respectively, as analyzed by chiral HPLC.