共查询到20条相似文献,搜索用时 203 毫秒
1.
植物组织培养技术被列入高中生物学选修模块“生物技术实践”内容中。依据实验教学实践,从应用价值、培养周期、激素调控规律方面,探讨了矮牵牛作为植物组织培养教学实验材料的优势。 相似文献
2.
几种观赏花卉的组织培养 总被引:1,自引:0,他引:1
自1982年来,我园用组织培养的方法对君子兰、五星花、重瓣矮牵牛、贴梗海棠、药百合等观赏花卉进行了实验,均取得了成功,其中部份试管苗移栽土壤成功,并已开花。下面将实验结果小结如下: 1.君子兰Clivia nobilis 相似文献
3.
体细胞无性系变异是植物组织培养中的一种普遍现象.常见的有染色体数目和结构变异、序列变异、DNA甲基化变异、基因的活化与沉默等。转座子和逆转录转座子的激活表明通过组织培养发生表观遗传变异。综述了植物组织培养中体细胞无性系变异的研究进展.重点阐述表观遗传变异在植物体细胞无性系变异中的作用。 相似文献
4.
大量试验证明,组织培养过程中可以产生许多变异。Burk和Matzinger首先在烟草的46个体细胞再生植株中,发现了广泛的表型变异。尔后,其他作物如甘蔗、小麦等,均发现有体细胞再生植株的变异。在水稻组织培养研究中,Oono首先报道了利用水稻去壳种子诱导愈伤组织进而分化成的再生植株,在株高、抽穗期、小穗育 相似文献
5.
6.
7.
8.
植物体细胞无性系变异是植物组织培养中的普遍现象,关于这些变异的起源存在多种观点,如转座因子的活化、DNA甲基化等。本文综述了植物体细胞无性系的研究进展,从细胞学和分子生物学两个层次对无性系变异的起源进行了讨论。 相似文献
9.
植物体细胞无性系变异的细胞学和分子生物学研究进展 总被引:25,自引:0,他引:25
植物体细胞无性系变异是植物组织培养中的普遍现象,关于这些变异的起源存在多种观点,如转座因子的活化、DNA甲基化等。本文综述了植物体细胞无性系的研究进展,从细胞学和分子生物学两个层次对无性系变异的起源进行了讨论 相似文献
10.
利用电离辐射诱发变异并进行选择,作为一种新的育种手段已广泛应用于农业生产,并显示出巨大的经济效益。近年来,利用植物组织培养及所产生的愈伤组织进行抗性变异系的诱导和选择,进而进行特异蛋白质的分离和抗性基因的克隆,已成为植物细胞工程和基因工程中十分活跃的新领域,尤其是对于耐盐变异系的选择引起了不少学者的关注。但利用γ 相似文献
11.
12.
13.
矮牵牛花药培养及植株再生研究 总被引:21,自引:1,他引:20
采用花粉发育双核期的矮牵牛花药,研究不同浓度植物生长调节剂配比及不同浓度蔗糖和麦芽糖对花药诱导率的影响。结果表明,采用6—BA和IBA组合诱导效果较好;NH 6—BAl.5mg/L IBA1.5mg/L花药诱导率较高;麦芽糖诱导效果明显比蔗糖好。 相似文献
14.
Endophytes are fungi that cause asymptomatic infections in leaves and stems of healthy plants. This study evaluated the presence of endophytic fungi on leaves of Rosa hybrida collected in Bogota, (Colombia) using histological techniques and light microscopy. Histological preparations showed fungal mycelium with few cells in the vascular tissue. Colonization of mesophyll cells was not observed. Visualization of fungal cells within plant tissues is a confirmatory test of the endophytic habitat of fungi isolated from leaves of Rosa hybrida. 相似文献
15.
Cytoplasmic hybrids (cybrids) between the two sexually incompatible species Nicotiana tabacum and Petunia hybrida were constructed.
Three green plants were obtained after fusion of leaf protoplasts from a cytoplasmic chlorophyll deficient mutant of tobacco,
with iodoacetamide inactivated protoplasts of P. hybrida. All regenerated plants were phenotypically similar to tobacco, but
male and female sterile. Chromosome and isoenzyme analyses of the nuclear genome, and restriction and blot hybridization analyses
of the organelle composition revealed that the regenerated cybrids possessed nuclear genome of N. tabacum, chloroplasts from
P. hybrida and recombinant chondriomes. In vitro culture of ovules from one cybrid plant pollinated by N. tabacum resulted
in the regeneration of cytoplasmic male sterile progeny plants. Cross-section of anthers from these CMS plants showed that
male sterility was due to a failure of tapetum development.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
16.
微型月季愈伤组织诱导及植株再生 总被引:2,自引:0,他引:2
选取微型月季(Rosa hybrida)幼嫩离体叶片(叶龄12天)为外植体, 以MS为基本培养基, 研究不同配比的植物生长调节剂对愈伤组织诱导、不定芽诱导和生根培养的影响。结果表明: (1) 诱导愈伤组织的最佳培养基配比为1.0 mg·L–16-BA+3.0 mg·L–12,4-D, 诱导率为100%; (2) 6-BA在微型月季不定芽诱导中起着关键作用, 诱导不定芽产生的植物生长调节剂配比为1.0 mg·L–16-BA +(0.05–0.5) mg·L–1NAA+(0.02–0.2) mg·L–1TDZ, 其中最适配比1.0 mg·L–16-BA+0.1 mg·L–1NAA+0.02 mg·L–1TDZ的愈伤组织再分化率达到92.6%; (3) 生长素对微型月季的生根有重要影响, 以1/4MS+0.1 mg·L–1NAA为生根培养基可获得100%的生根率。实验最终建立了微型月季组织培养和高频离体再生体系。 相似文献
17.
18.
19.
组织培养导致的草莓DNA甲基化变异 总被引:1,自引:0,他引:1
以草莓品种‘丰香’和‘全明星’为材料,用甲基化敏感扩增多态性(MSAP)技术研究组织培养对草莓DNA甲基化的影响。结果表明,与普通苗相比,组织培养导致草莓试管苗的DNA甲基化水平下降,甲基化模式的变异以去甲基化为主。组织培养导致的DNA甲基化变异不稳定,在田间无性繁殖过程中,试管苗的无性繁殖后代DNA甲基化水平逐渐升高,仅部分变异的甲基化模式能够在试管苗的无性繁殖后代中稳定传递。两个品种之间,纽织培养对DNA甲基化变异程度的影响不同。 相似文献
20.
A method is described for the determination of in vivo ubiquinone (UQ) reduction levels in nongreen tissues by extraction and subsequent detection of ubiquinone-10 and ubiquinol-10 with high-performance liquid chromatography. In Petunia hybrida cell suspensions UQ reduction remained at a stable level of about 60%, despite the changing conditions during the batch culture (from excess sugar to starvation) and the concomitant variations in respiration. Also, in the presence of uncoupler, which causes a large increase in respiration via both the cytochrome pathway and the alternative pathway, UQ reduction levels stayed at 60%. In mitochondria isolated from these cells, activity of the alternative pathway was only observed at UQ reduction levels higher than 80%. It is proposed that in vivo the relationship between UQ reduction and the activity of the alternative oxidase is modulated by mechanisms such as thiol modifications and accumulation of organic acids. Accordingly, pyruvate concentration in P. hybrida cells increased in the presence of uncoupler. 相似文献