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1.
木霉T6木聚糖酶制剂研究   总被引:2,自引:0,他引:2  
本文研究了木霉T6(Trichoderma sp.)产木聚糖酶固态发酵过程和木聚糖酶制剂制备,结果显示,固体曲培养4d时酶活力最高,固体曲最适液固浸提比为7:1。木聚糖酶在60~65%硫酸铵饱和度下盐析效果最好。冷冻干燥和40℃烘干酶粉得率分别为68.3%和45.7%。酶最适反应PH为4.5,最适反应温度50℃,在不同温度下1小时后的半失活温度为47.7℃。  相似文献   

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青霉菌木聚糖酶粗酶制剂制备和性质   总被引:3,自引:1,他引:2  
本文研究了青霉菌P1固态发酵培养的产酶过程,发酵4天产木聚糖酶活力最高,固体曲最适浸提比为1:7(w/v),通过65%饱和度的硫酸铵盐析,获得的木聚糖酶效果较好。40℃烘干酶粉活力为1305.6IU/g,得率为69.5%。酶反应最知适pH4.6,最适温度50℃。酶与底物半纤维素作用的亲合性大小顺序为:麸皮〉稻草〉蔗粉〉玉米蕊。  相似文献   

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曲霉木聚糖酶发酵条件与性质   总被引:14,自引:0,他引:14  
从实验室培养污染物上分离出一株产木聚糖酶活力高的曲霉(AspergillusSp.)A3菌株。研究了其发酵过程,该菌经30℃,培养96小时,酶活力可达320IU/ml,研究了碳源,氮源,发酵起始pH值及通风量对产酶的影响。酶的最适反应温度为55℃,最适pH值为4.4。在不同温度下保温1小时,测得该酶的半失活温度为51℃。  相似文献   

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纤维素酶酶解稻壳的条件试验   总被引:22,自引:0,他引:22  
本文报道康氏木霉N-78(Trichodermakoningi)纤维素酶产生和酶解稻壳的适宜条件。实验结果表明,在稻草粉麸皮固体培养基上,纤维素酶产生的适宜条件为稻草粉和麸皮的比例为7:3,培养基含水量为250%,pH为6.0—6.5,温度为30℃,时间为3d。酶解稻壳的最适条件为:pH为4.4,温度为40℃,作用时间为3d,酶曲量和底物量比例为1:3。  相似文献   

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产纤维素酶菌株宇佐美曲霉Y-11产酶条件及酶性质的研究   总被引:4,自引:0,他引:4  
通过正交试验,对宇佐美曲霉纤维素酶固态发酵条件进行了研究,其较适培养基为:麸皮与稻草配比为2:3,氮源:尿素,含水量:200%(液固比),pH:5.5。28-30℃培养72小时,CMC酶活力为5.05(u/ml),滤纸酶活力达到1.04(u/ml)。CMC酶及滤纸酶最适pH分别为4.41、6.06;最适温度分别为65℃、55℃。酶的热稳定性与pH稳定性较高。  相似文献   

6.
培养基初始pH值对木聚糖酶合成的影响   总被引:4,自引:0,他引:4  
洪枫  陈琳 《工业微生物》1999,29(4):15-18
本文探讨了培养基三种初始pH值对木聚糖酶合成的影响。试验结果表明,培养基初始pH值对木聚糖酶的合成有重大影响,低pH有利于提高木聚糖酶活力。在碳源为7g/L条件下,初始pH值为4.0时合成的木聚糖酶活力高达32.87IU/ml,酶产率和得率分别为6574.0IU/L·d和4695.7IU/g木聚糖。酶活力和酶产率是初始PH5.0的1.7倍,分别是初始PH6.0的3.8倍和2.3倍。进一步研究表明,  相似文献   

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甜瓜的组织培养与快速繁殖   总被引:11,自引:0,他引:11  
1植物名称甜瓜(Cucumismelo)品种“状元”。2材料类别顶芽、侧芽。3培养条件初代培养基:(1)MS+6-BA0.2mg·L-1(单位下同)+IAA0.2。增殖培养基:(2)MS+6-BAI+IAA0.2。生根培养基:(3)Miller+IAA0.2;(4)Miller(无激素)。以上培养基pH均为5.8~6.0。琼脂浓度在(1)、(2)中为0.7%,在(3)、(4)中为0.6%;糖浓度在(1)、(2)中为3%,在(3)、(4)中为2%。培养温度均为(25±3)℃,光照度2000lx,光…  相似文献   

8.
多孔菌液体发酵的研究   总被引:1,自引:0,他引:1  
多孔菌Polyprouselegans(Bull)Fr。是一种新开发的真菌类中药材资源,经对其几个主要液体发酵培养条件的筛选研究,初步得出多孔菌深层发酵的基础参数:PH5.6~0.1;温度24~28℃;培养基为麦麸2.5%,蔗糖4%,NH4NO30.05%,KH:PO40.05%,MgSO4·7H2O0.025%。另外,摇瓶种子培养条件是:PH5.6~6.1;温度28℃;培养基为PDA+VB1,添加3‰琼脂,时间为96hr。  相似文献   

9.
酸性木聚糖酶产生菌的筛选及产酶条件   总被引:21,自引:0,他引:21  
从150株真菌中筛选到8株产木聚糖酶活力在100U/mL以上的菌株,其中活力最高的为黑曲霉(编号149)(Aspergilusniger)。该菌株产酶较适培养基为:麸皮半纤维素4%,NaNO31%,麸皮1%,用不加(NH4)2SO4和尿素的Mandels氏营养盐液配制。28℃~30℃振荡培养60h,酶活力最高可达375.2U/mL。该酶最适作用pH为46,在pH3~11之间基本稳定。该菌株发酵液中含有木聚糖酶(相对活力100)外还有淀粉酶(18),甘露聚糖酶(098),β木糖苷酶(094)和纤维素酶(017)。  相似文献   

10.
筛选得到一株高产β-1,4-聚糖酶的耐碱性芽孢杆菌A-30,其固体发酵(Solid state fermentationSSF)时最适培养条件:起始pH为8.0、培养温度32℃、10%(v/w)接种量,含水量为66.6%(v/w),以0.5%的NaNO_3为无机氮源,发酵96h,木聚糖酶活可达6457IU/g(Dry bacterial bran),纤维素酶活(CMCase)可以达到18.66IU/g。氮源组成、温度、pH及发酵时间等对A-30所产木聚糖酶和纤维素酶比例有较大影响。葡萄糖对A-30的阻遏效  相似文献   

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Comprises species occurring mostly in subtidal habitats in tropical, subtropical and warm-temperate areas of the world. An analysis of the type species, V. spiralis (Sonder) Lamouroux ex J. Agardh, a species from Australia, establishes basic characters for distinguishing species in the genus. These characters are (1) branching patterns of thalli, (2) flat blades that may be spiralled on their axis, (3) width of the blade, (4) primary or secondary derivation of sterile and fertile branchlets and (5) position of sterile and fertile branchlets on the thalli. Application of the latter two characters provides an important basic method for separation of species into three major groups. Osmundaria , a genus known only in southern Australia, was studied in relation to Vidalia , and its separation from the Vidalia assemblage is not accepted. Species of Vidalia therefore are transferred to the older genus name, Osmundaria. Two new species, Osmundaria papenfussii and Osmundaria oliveae are described from Natal. Confusion in the usage of the epithet, Vidalia fimbriala Brown ex Turner has been clarified, and Vidalia gregaria Falkenberg, described as an epiphyte on Osmundaria pro/ifera Lamouroux, is revealed to be young branches of the host, Osmundaria prolifera.  相似文献   

16.
Fifteen chromosome counts of six Artemisia taxa and one species of each of the genera Brachanthemum, Hippolytia, Kaschgaria, Lepidolopsis and Turaniphytum are reported from Kazakhstan. Three of them are new reports, two are not consistent with previous counts and the remainder are confirmations of very scarce (one to four) earlier records. All the populations studied have the same basic chromosome number, x = 9, with ploidy levels ranging from 2x to 6x. Some correlations between ploidy level, morphological characters and distribution are noted.  相似文献   

17.
Curcumin is the yellow pigment of turmeric that interacts irreversibly forming an adduct with thioredoxin reductase (TrxR), an enzyme responsible for redox control of cell and defence against oxidative stress. Docking at both the active sites of TrxR was performed to compare the potency of three naturally occurring curcuminoids, namely curcumin, demethoxy curcumin and bis-demethoxy curcumin. Results show that active sites of TrxR occur at the junction of E and F chains. Volume and area of both cavities is predicted. It has been concluded by distance mapping of the most active conformations that Se atom of catalytic residue SeCYS498, is at a distance of 3.56 from C13 of demethoxy curcumin at the E chain active site, whereas C13 carbon atom forms adduct with Se atom of SeCys 498. We report that at least one methoxy group in curcuminoids is necessary for interation with catalytic residues of thioredoxin. Pharmacophore of both active sites of the TrxR receptor for curcumin and demethoxy curcumin molecules has been drawn and proposed for design and synthesis of most probable potent antiproliferative synthetic drugs.  相似文献   

18.
正Dear Editor,In December 2019, a novel human coronavirus caused an epidemic of severe pneumonia(Coronavirus Disease 2019,COVID-19) in Wuhan, Hubei, China(Wu et al. 2020; Zhu et al. 2020). So far, this virus has spread to all areas of China and even to other countries. The epidemic has caused 67,102 confirmed infections with 1526 fatal cases  相似文献   

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