鳖扁蜡蝉和娇弱鳎扁蜡蝉雌性生殖器的描述(半翅目,蜡蝉总科,扁蜡蝉科)

对扁蜡蝉科(半翅目,蜡蝉总科)中鳖扁蜡蝉Cixiopsis punctatus Matsumura和娇弱鳎扁蜡蝉Tambinia debilis St(a)l的雌性生殖器进行了详细描述,绘制特征图并指明各部分的结构名称.结果表明前者生殖管道属于单开口类型,而后者是双开口类型.雌性生殖器特征可为分类学和系统发育分析提供更多的参考信息.     

中国鳎扁蜡蝉属分类研究(半翅目:蜡蝉总科:扁蜡蝉科)

对中国鳎扁蜡蝉属Tambinia St(a)l的种类进行了研究,并记述采自云南勐仑的该属1新种:勐仑鳎扁蜡蚪Tambinia menglunensis Men & Qin,sp.nov..提供了雄虫整体照片,绘制了雌、雄外生殖器特征图,编制了国内已知种分种检索表.模式标本保存于西北农林科技大学昆虫博物馆.     

扁足瓢蜡蝉属分类研究(半翅目,蜡蝉总科,瓢蜡蝉科)

对扁足瓢蜡蝉属Neodurium Fennah(半翅目,蜡蝉总科,瓢蜡蝉科)进行了重新描记,该属目前已知3种,其中2新种,即指扁足瓢蜡蝉Neodurium digitiformum Ran et Liang,sp.nov.和平扁足瓢蜡蝉Neodurium flatidumRan et Liang,sp.nov..新种模式标本保存在中国科学院动物研究所.     

中国扁足瓢蜡蝉属订正及一新种记述(英文)

对瓢蜡蝉科Issidae扁足瓢蜡蝉属Neodurium Fennah进行了订正。记述中国扁足瓢蜡蝉属4种,包括1新种,钩扁足瓢蜡蝉Neodurium hamatum sp.nov.,提供了形态及雌雄性外生殖器等特征图。制作了本属分布于中国的7个种的分种检索表。钩扁足瓢蜡蝉,新种N.hamatum sp.nov.(图1~24)新种与Neodurium postfasciatum Fennah相近似,其区别主要在于:前翅有爪缝,但端部较模糊,肛节端部不弯曲,阳茎具1对钩状突起。新种模式标本保存在西北农林科技大学昆虫博物馆。正模♂,云南勐仑,2009-07-23,张磊采。副模1♀,云南勐仑,2009-07-12,张磊采。词源:新种种名据其阳茎具钩状突起而定名。     

中国鳎扁蜡蝉属一新记录种

报道采自中国海南省和云南省的扁蜡蝉科鳎扁蜡蝉属Tambinia Stal 1中同新记录种:似鳎扁蜡蝉T.similis Liang,2003,对雌性外生殖器进行了首次描述,并附中国已知种检索表.研究标本保存于西北农林科技大学昆虫博物馆.     

中国鳎扁蜡蝉属——新记录种

报道采自中国海南省和云南省的扁蜡蝉科鳎扁蜡蝉属Tambinia Stal 1中国新记录种：似鳎扁蜡蝉T.similis Liang,2003,对雌性外生殖器进行了首次描述,并附中国已知种检索表。研究标本保存于西北农林科技大学昆虫博物馆。     

帕萨瓢蜡蝉属综述及中国一新种记述（半翅目：蜡蝉总科：瓢蜡蝉科）

对帕萨瓢蜡蝉属的研究进行了综述并修订了属征,报道采自中国湖北和广西的帕萨瓢蜡蝉属1新种:三叶帕萨瓢蜡蝉Parasarima triphylla sp. nov.。该新种与帕萨瓢蜡蝉Parasarima pallizona(Matsumura)相似,主要区别为:1)前翅M脉3分支,后者2分支;2)后足刺式,前者6-7-2,后者7-9-2;3)抱器的突起基部尖细、端部钝圆,后者突起的基部和端部钝圆。研究标本保存在西北农林科技大学昆虫博物馆。     

中国鼻象蜡蝉属一新种记述(半翅目:蜡蝉总科:象蜡蝉科)

记述采自贵州省贵阳森林公园和绥阳县宽阔水的鼻象蜡蝉属Saigona Matsumura 1新种,即囊突鼻象蜡蝉 Saigona saccus sp.nov..新种近似于瘤鼻象蜡蝉Saigona fulgoroides(Walker,1858),主要区别在阳茎基的结构上.前者阳茎基宛如一套子包裹在阳茎外面;后者阳茎基从侧面看,端部背面有一背部延伸的叶状突起,腹面具有小的叶状突起.详细描述了新种的外部形态特征,绘制了雄虫鉴别特征图.模式标本保存在贵州大学昆虫研究所.     

中国新纪录属漪扁蜡蝉属及一新种记述(半翅目,蜡蝉总科,扁蜡蝉科)

记述中国扁蜡蝉科1新纪录属和1新种:漪扁蜡蝉属Isporisa Walker,1857,背崩漪扁蜡蝉Isporisa baibungensis sp.nov..模式标本保存于中国科学院动物研究所,北京.背崩漪扁蜡蝉,新种Isporisa baibungensis sp.nov.(图1～11)正模♀,西藏墨脱背崩,870 m,1979-11-07,金银桃、吴建毅采(IZCAS). 新种与I.apicalis Walker ,1857相似,两者主要区别:前 者体长9.0 mmm,后者体长6.4 mm;前者顶中脊未伸达到眼前缘,头顶凹陷外侧缘具2浅凹坑,后者顶中脊超过眼前缘,头顶凹陷外侧缘无凹坑;前者中胸背板后缘具4黑斑,后者中胸背板后缘具2黑斑.     

中国瓢蜡蝉科一新记录属

报道中国新记录属广瓢蜡蝉属Macrodaruma Fennah及中国新记录种广瓢蜡蝉M.pertinax Fennah,绘制了其主要外形特征及雄性外生殖器图,首次描记了雌虫的主要外形特征。     

褐飞虱长翅型和短翅型雌雄成虫体内磁性物质定位和定量检测

【目的】地磁定向是昆虫远距离迁飞定向的重要机制之一。本研究以迁飞性害虫褐飞虱Nilaparvata lugens长翅型和短翅型雌雄成虫为研究对象,系统开展虫体内磁性物质定量研究。【方法】本研究利用MPMS-7型超导量子磁强计检测褐飞虱长翅型和短翅型雌雄成虫体内的磁性物质,并将经普鲁士蓝染色后的虫体超薄石蜡切片于JEM-2100型透射电子显微镜下观察磁性物质的分布状况,最后利用原子发射光谱法对虫体内的磁性物质进行定量分析与比较。【结果】超导量子磁强计检测发现,仅在褐飞虱长翅型和短翅型雌雄成虫腹部的磁滞曲线有明显闭合现象,证明该部位存在磁性物质;经透射电镜观察发现,普鲁士蓝沉淀主要呈点簇状存在于虫体腹部,证明该部位存在铁磁性物质;通过等离子发射光谱检测发现,同一性别长翅型成虫体内铁离子含量显著高于短翅型成虫,且相同翅型雄成虫体内铁离子含量显著高于雌成虫。【结论】褐飞虱长翅型和短翅型雌雄成虫腹部普遍存在铁磁性物质,且不同翅型和性别间磁性物质含量差异显著。     

稻飞虱翅型纯系后代个体的翅型分化对光周期变化不敏感

【目的】为了明确光周期和遗传因子在稻飞虱翅型分化中的作用, 研究了3种稻飞虱（褐飞虱Nilaparvata lugens、白背飞虱Sogatella furcifera和灰飞虱Laodelphax striatellus）翅型纯系或近纯系在不同光照时数下的翅型分化比率。【方法】以经过5~45代连续翅型筛选后的褐飞虱、白背飞虱和灰飞虱的长翅型和短翅型纯系或近纯系为材料, 在室内分别测定了其在长光照（16和20 h）、短光照(4~12 h)和正常光照(14 h) 3类光周期条件下饲养后, 雌、雄成虫中长翅和短翅个体出现的比率及存活率。【结果】白背飞虱和灰飞虱的长翅型纯系M♂×M♀或短翅型纯系B♂×B♀在不同光周期下的翅型比率均无显著差异（P>0.05）。褐飞虱短翅型近纯系B♂×B♀的雌虫短翅率和成虫总短翅率在不同光周期下也无显著差异（P>0.05）, 但雄虫短翅率在正常光照14 h和短光照4 h下显著高于长光照20 h下的（P<0.05）。当褐飞虱短翅型达到纯系后, 其后代翅型在6~16 h光照条件下无显著差异。褐飞虱长翅型近纯系M♂×M♀的后代虽有短翅个体出现, 但是雌虫和雄虫的各自短翅率在不同光周期下无显著差异（P>0.05), 仅总体短翅率在12 h光照条件下的显著高于16 h下的（P<0.05）。褐飞虱长、短翅型杂交筛选品系M♂×B♀的雌虫短翅率随光照时数的延长而升高; 灰飞虱杂交筛选品系M♂×B♀的短翅雄虫随光照时数的缩短而增多（P<0.05）, 但当筛选代次达到45代时, 这种趋势不再显著。3种稻飞虱长翅型和短翅型纯系或近纯系若虫的存活率会稍低于长、短翅型杂交后代的存活率, 但长、短翅型品系的存活率在6~16 h光照条件下差异不显著(P>0.05)。【结论】稻飞虱翅型分化对光周期的反应受飞虱本身遗传背景的影响, 翅型纯系后代个体的翅型分化对光周期变化不敏感。     

高温对稻褐飞虱发育与生殖的影响

通过高温处理及交配产卵实验,探讨了高温对稻褐飞虱Nilaparvata lugens (Stal)发育与生殖的影响。研究了不同高温条件对褐飞虱若虫发育历期、产卵量、产卵前期、寿命等生物学特性的影响。34℃以上高温导致褐飞虱若虫发育历期延长。高温处理4龄若虫使羽化后的雌成虫产卵量减少。高温处理不同日龄雌成虫也致使其产卵量减少,其中以1日龄短翅型、3日龄长翅型的产卵量影响最大。高温处理后褐飞虱寿命缩短。高温恒温处理,对短翅型雌成虫的产卵前期影响不大,但能延长长翅型雌成虫的产卵前期;而高温变温处理致使短、长翅型雌成虫的产卵前期均延长。高温变温对褐飞虱生殖的影响程度大于高温恒温。高温处理组雌、雄成虫与对照组相应的成虫交配试验表明,高温对雌性的影响大于雄性。实验初步确定34℃为对褐飞虱发育与生殖产生影响的临界温度。     

近零磁场对褐飞虱翅型分化、趋光性及飞行能力的影响

【目的】地磁场(geomagnetic field,GMF)不是恒定不变的,而是随时间和空间时刻变化的。目前,动物对磁场变化的响应研究主要集中于迁徙性动物地磁定向导航中的磁感受方面,而迁徙性动物迁出地和迁入地之间地磁场强度差异对动物生理和行为潜在的磁场效应尚不明确。【方法】迁飞性昆虫褐飞虱Nilaparvata lugens试虫采自江苏省农业科学院试验田。本文采用亥姆霍兹线圈室内模拟近零磁场(near-zero magnetic field,NZMF)和GMF,调查了褐飞虱成虫在NZMF和GMF下的翅型分化、趋光性和飞行能力。【结果】结果表明,与GMF相比,NZMF显著提高了褐飞虱雄成虫短翅型比例(6. 4%)(P <0. 05),但对雌成虫长翅型比例影响不显著(P> 0. 05);对于长翅雌成虫,NZMF显著提高了其2日龄成虫的上灯比例(55%)(P <0. 05),但显著降低了其4日龄成虫的上灯比例(22%)(P <0. 05),对趋光性的影响总体呈现出随着龄期先增强后减弱的效应。NZMF对长翅雄成虫趋光性的影响也呈现出相同的效应,但对各日龄成虫的影响不显著(P> 0. 05); NZMF显著缩短了2日龄长翅雄成虫的飞行时间(46%)(P <0. 05),并显著提高了长翅雌成虫(65%)和长翅雄成虫(101%)的飞行速度(P <0. 05)。此外,GMF对照组的褐飞虱长翅雄成虫飞行速度显著低于长翅雌成虫(96%),而NZMF处理组中二者无显著差异。【结论】结果说明,近零磁场可提高褐飞虱成虫短翅比例,对长翅成虫趋光性的影响呈现出基于龄期增长先增强后减弱的效应,并在未影响长翅成虫飞行距离的情况下,改变了其飞行策略,即提高飞行速度,同时缩短飞行时间。     

Differences in haemolymph proteins in relation to diapause and wing dimorphism inPyrrhocoris apterus (L.) (Heteroptera: Pyrrhocoridae)

Three different cultures of dimorphic bug,Pyrrhocoris apterus, were analyzed concerning diapause and its relation to wing morph pattern. The proportion of macropterous bugs was considerably higher (36%) in the Mediterranean culture from Israel than that (1.3%) in the temperate culture from the Czech Republic. The macropterous morph- and brachypterous morphrelated types of reproduction arrest, differing by the length of pre-oviposition period, were distinguished in cultures analyzed. The reproduction arrest with an average pre-oviposition period of 38.2 days in Mediterranean macropters and 18.5 days in macropters from selected macropterous strain, was found to be typical for macropterous morph. Two different photoperiodic conditions induced macropterous morph-related reproduction arrest, the long-day (18 h light-6 h dark) photoperiod in macropterous strain macropters and the short-day (12 h light-12 h dark) photoperiod in Mediterranean macropters. The brachypterous morph-related reproduction arrest, characterized by pre-oviposition period longer than 90 days, occurred predominantly in diapausing brachypterous bugs. While the hibernal diapause of brachypterous bugs was characterized by a very high level of the 78- and 82-kDa proteins in haemolymph, their content in haemolymph of macropters during macropterous morph-related reproduction arrest was almost as low as in the reproductive adults. The variation of reproduction arrest in relation to wing dimorphism represents an important feature in the life strategy ofP. apterus.     

A male fitness advantage to wing reduction in the oriental chinch bug,Cavelerius saccharivorus okajima (heteroptera: Lygaeidae)

A male fitness advantage to wing reduction was investigated for the oriental chinch bug, Cavelerius saccharivorus, which is wing dimorphic. Field surveys for the frequency of matings between wing morphs showed that the mating probability was much higher in brachypterous males than in macropterous males in the early breeding season. Brachypterous males copulated with not only brachypterous females but also macropterous females in this season. This led to a considerable rate (30%) of insemination of macropterous females just before emigration even in the early breeding season. A rearing experiment for the pre-reproductive period of females revealed that females mated with brachypterous males copulated and oviposited earlier than females mated with macropterous males. These results indicate that there is a fitness advantage to wing reduction in the males of C. saccharivorus in terms of the earlier sexual maturation.     

褐飞虱翅型分化的组织学研究

利用石蜡切片的方法,观察并比较了褐飞虱Nilaparvata lugens (Stal)长翅型和短翅型纯系各龄若虫及成虫的翅芽和间接飞行肌的发育情况。发现在4龄的第8h以后间接飞行肌就开始分化,长翅型若虫的间接飞行肌正常发育而短翅型若虫间接飞行肌的发育则被抑制。在5龄初始时方可明显观察到翅芽分化,短翅型若虫前翅芽细胞增殖速度明显慢于长翅型,并且其后翅芽停止发育。本文还比较了长翅型雌、雄性个体之间间接飞行肌的发育情况。     

Molecular characterization of the flightin gene in the wing-dimorphic planthopper,Nilaparvata lugens,and its evolution in Pancrustacea

Flightin was initially identified in Drosophila melanogaster. Previous work has shown that Drosophila flightin plays a key role in indirect flight muscle (IFM) function and has limited expression in the IFM. In this study, we demonstrated that flightin is conserved across the Pancrustacea species, including winged insects, non-winged insects, non-insect hexapods and several crustaceans. The brown planthopper (BPH), Nilaparvata lugens (Stål) (Hemiptera: Delphacidae), a long-distance migration insect with wing dimorphism, is the most destructive rice pest in Asia. We showed that flightin was one of the most differentially expressed genes in macropterous and brachypterous BPH adults. In female BPHs, flightin was expressed in the IFM of macropterous adults, no expression was detected in brachypterous ones; while in male BPHs, flightin was not only expressed in the IFM of macropterous adults, but also in the dorsal longitudinal muscle (DLM) in the basal two abdominal segments of both macropterous and brachypterous ones. RNAi and transmission electron microscopy results showed that flightin played key roles in maintaining IFM and male DLM structure, which drive wing movements in macropterous adults and the vibration of the male-specific tymbal, respectively. Using Daphnia magna as an example of a crustacean species, we observed that flightin was expressed in juvenile instars and adults, and was localized in the antenna muscles. These results illustrate the functional variations of flightin in insects and other arthropod species and provide clues as to how insects with flight apparatuses evolved from ancient pancrustaceans.     

Wing structure and polymorphism in minute scavenger beetles (Coleoptera,Latridiidae)

The main structural elements of the hind wing in 56 species of the family Latridiidae (Coleoptera) are analyzed. In macropterous forms, wing venation is reduced to 3 modified veins. 10 areas of sclerotization of the wing membrane are distinguished. Three forms with different degrees of wing reduction are revealed: the brachypterous, the micropterous, and the apterous form. Among 56 species examined, 40 are represented only by the macropterous form; 2, 3, 5, and 6 species, by macropterous and brachypterous, macropterous and micropterous, micropterous, and apterous forms, respectively.