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【目的】果实蝇属Bactrocera中有国际上重要的检疫性害虫, 基于形态的物种鉴定有一定的局限性。另一方面, 云南边境地区为东南亚地区实蝇入侵我国的重要通道。因此, 对该地区实蝇分子鉴定方法的研究对于该属物种的快速准确鉴定具有重要意义。本研究旨在探讨DNA条形码技术在果实蝇属物种鉴定中的有效性。【方法】使用线粒体基因COI和COII序列的通用引物对果实蝇属20个物种60份样品进行PCR扩增、测序和序列分析; 采取距离方法和建树方法评价2种序列的鉴别能力。【结果】COI和COII序列平均长度分别为682 bp和339 bp, 种内和种间遗传差异较大, 有较明显的遗传距离间隔(barcoding gap), 鉴定成功率分别为91.2%和90.7%。另外, 分子系统树表明华实蝇亚属Sinodacus不是单系群。【结论】COI和COII序列均能够将绝大多数果实蝇属物种进行准确鉴别, 应用COI或COII序列进行果实蝇属物种鉴定具有一定的可行性。 相似文献
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本文记述采自川西北地区泉蝇属一新种——黄龙泉蝇Pegomya huanglongensis 1♂及邻泉蝇属一新纪录种——兰得贝克邻泉蝇Paradelia,lundbeckii,并附Ⅱ、Ⅳ、Ⅴ腹板及尾器图。 相似文献
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对中国角潜蝇属莎草潜蝇亚属进行了分类研究,确认我国现知2种:即福建角潜蝇C.(B.)fujianisca sp.nov和苔角潜蝇Cerodontha(Butomomyza)cornigera(dc Meijcrc)。除新种描述并附特征图外,还提供该亚属中国种类分种检索表。模式标本保存于中国科学院动物研究所动物标本馆。 相似文献
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对缟蝇科同脉缟蝇属Homoneura4种、黑缟蝇属Minettia和双鬃缟蝇属Sapromyza各1种的异物同名进行了修订,并建议给以新名,即Homoneura(Homoneura) conjunctiva nom.nov.,Homoneura(Homoneura) oceaniensis nom.nov.,Homoneura(Homoneura) laoensis nom.nov.,Homoneura(Homoneura) singaporica nom.nov.,Minettia(Minettiella) sasakawai nom.nov.,Sapromyza(Sapromyza) sasakawai nom.nov.。观察标本保存于中国农业大学昆虫标本馆。 相似文献
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四川省阳蝇属三新种:双翅目:蝇科 总被引:1,自引:0,他引:1
阳蝇属三新种,双鬃阳蝇,新种Helinaduplicisetasp.nov;大斑阳蝇,新种Helinamagnimuculatasp.nov。欢愉阳蝇,新种Heliniahestasp.nov。模式标本I号存华西医科大学,Ⅱ,Ⅲ号存在于雅安地区卫生防疫站。 相似文献
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毛盾圆蝇属Lasiopelta Malloch,1928是个小属,迄今仅发现于东洋区。笔者在整理采自贵州的蝇类标本中,发现该属一新种。新种的模式标本保存于作者所在单位。斑翅毛盾圆蝇Lasiopelta maculipennis新种 雄 体长5mm。头底色黄,仅后头上部蓝黑色。眼具极疏微毛,几乎裸。额颇宽,头顶部约为一眼宽。间额暗红,具极薄的灰黄色粉被。额三角粉被金黄,细长,前段狭如线, 相似文献
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本文记述采自我国山西省的棘蝇属Phaonia R。-D。,1830一新种:方山棘蝇Phaonia fangshanensis,sp.nov。模式标本保存在沈阳师范学院昆虫研究所。 相似文献
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Phylogenetic relationships among Bactrocera species (Diptera: Tephritidae) inferred from mitochondrial DNA sequences 总被引:5,自引:0,他引:5
Several members of the dipteran family Tephritdae are serious pests because females lay eggs in ripening fruit. The genus Bactrocera is one of the largest within the family with over 500 described species arranged in 28 subgenera. The phylogenetic relationships among the various species and subgenera, and the monophyly of specific groups have not been examined using a rigorous phylogenetic analysis. Therefore, phylogenetic relationships among 24 Bactrocera species belonging to 9 subgenera were inferred from DNA sequence of portions of the mitochondrial 16S rRNA, cytochrome oxidase II, tRNA(Lys), and tRNA(Asp) genes. Two morphological characters that traditionally have been used to define the four groups within the subgenus Bactrocera were evaluated in a phylogenetic context by mapping the character states onto the parsimony tree. In addition, the evolutionary trend in male-lure response was evaluated in a phylogenetic context. Maximum parsimony analyses suggested the following relationships: (1) the genus Bactrocera is monophyletic, (2) the subgenus B. (Zeugodacus) is paraphyletic, (3) the subgenus B. (Daculus) is a sister group to subgenus B. (Bactrocera), and (4) the subgenus B. (Bactrocera) is monophyletic. The mapping analyses suggested that the morphological characters exhibit a simple evolutionary transition from one character state to another. Male-lure response was identified as being a labile behavior that has been lost on multiple occasions. Cue-lure response was plesiomorphic to methyl-eugenol response, and the latter has evolved independently within the Bactrocera and Zeugodacus groups of subgenera. The implications of our results for devising a coherent, consolidated classification for Bactrocera is discussed. 相似文献
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A review of Polyphylla Harris, 1841, species belonging to the subgenus Granida Motschulsky, 1861, from continental Asia is presented. One new species is described from Thailand: Polyphylla (Granida) simonisp. n.Polyphylla (Granida) nikodymi de Wailly, 1993, is recorded from Thailand for the first time. Polyphylla (Granida) minor Nomura, 1977, is recorded from Yunnan (China) for the first time. The previously unknown female of Polyphylla (Granida) phongsali Zídek, 2006, is described. 相似文献
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Ickert-Bond SM 《International journal of plant sciences》2000,161(2):301-317
Cuticle micromorphology of the unusual Vietnamese pine, Pinus krempfii Lecomte, and three additional endemic southeast Asian species of Pinus L. (Pinaceae) is characterized for the first time. Taxa studied include (1) P. krempfii, typically placed in its own subgenus Ducampopinus (Chevalier) Ferré ex Little & Critchfield; (2) the endemic Vietnamese species Pinus dalatensis Ferré and (3) the southeast Asian species Pinus kwantungensis Chun ex Tsiang, both of subgenus Strobus; and (4) the widespread Asian species Pinus kesiya Royle ex Gordon of subgenus Pinus. The current and previous studies demonstrate that the genus Pinus and its subgenera are delimited by unique combinations of cuticular characters, although some of these characters may occur individually in other conifers. Cuticular micromorphology supports taxonomic assignment of P. krempfii to subgenus Strobus rather than to its own subgenus, a result that is also indicated by other anatomical studies and recent molecular studies. Sectional affinities of P. krempfii are usually with Parrya, subsection Krempfianae. An alternative classification of P. krempfii with subsection Gerardianae can be supported by micromorphological characters including broad cuticular bridges between stomata, details of the intercellular flanges of the epidermal cells, and usually an amphistomatic stomatal distribution. Features of other Asian species studied are consistent with their taxonomic assignments. The study demonstrates the utility of cuticle micromorphology to taxonomic delimitation within the family Pinaceae. 相似文献
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齿隐翅虫属Priochirus种类多见于腐木树皮下,是重要的腐木甲虫.其中斑齿隐翅虫亚属的物种比较稀少,目前全世界仅记录3种.本文记述产于我国云南的齿隐翅虫属斑齿隐翅虫亚属1新种,硕斑齿隐翅虫Priochirus(Stigma-tochirus)magnificus sp.nov,及1新纪录种,阿斑齿隐翅虫Priochirus(Stigmatochirus)abori Bernhauer.文中物种检索表包括了世界现有的3种及新种共4个种.所有标本材料(包括所有模式标本)都保存在中国科学院动物研究所. 相似文献
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记述果实蝇属Bactrocera Macquart 1新种:甘肃果实蝇Bactrocera(Zeugodacus)gansuica Chen,Han et Zhousp.nov.及老挝果实蝇属1新记录种:黑颜果实蝇B(Z.)vultus(Hardy,1973),并附形态特征图。新种和盾条果实蝇B.(Z.)adusta(Wang & Zhao)及塔帕果实蝇Bactrocera(B.)tappanus(Shiraki)相似,均仅具1对小盾鬃,中胸盾片除2侧纵条及1中纵条外,余均黑色,以及小盾片具1黑色宽纵条。主要区别在于:1)前足股节端半深棕色,而非几乎全黑;2)中、后足股节端部约1/3深棕色;3)翅前缘带与端斑明显相连,而非与端斑明显分离。 相似文献