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共有20条相似文献,以下是第1-20项 搜索用时 171 毫秒

1.  生物反应器填埋场系统渗滤液的脱氮性能  被引次数:1
   何若  沈东升  朱荫湄《应用生态学报》,2006年第17卷第3期
   利用填埋场垂直分布的好氧-缺氧-厌氧的独特生态环境,并采用填埋垃圾上层间歇曝气充氧的方式,研究了生物反应器填埋场系统渗滤液的脱N性能.结果表明,填埋垃圾上层间歇曝气充氧,促进了填埋垃圾层硝化细菌和反硝化细菌的生长,且可使反硝化细菌的数量比普通的填埋垃圾层高4~13个数量级,硝化细菌的最大数量可达到109个·g-1;营建了填埋场内硝化、反硝化等脱N反应的生物环境,有利于回流渗滤液含N化合物的去除.试验结束时,其渗滤液NH4+-N和TN浓度分别为186和289 mg·L-1,仅为对照的18%和26%.此外,填埋垃圾上层间歇曝气充氧也有利于填埋垃圾的降解,提高垃圾的稳定化效果.    

2.  Biodiversity and water quality variations in constructed wetland of Yongding River system  
   Liu Cao  Du Guisen  Huang Bingbin  Meng Qingyi  Li Huimin  Wang Zijian  Song Fu《生态学报》,2007年第9期
   This research was carried on in constructed wetlands of Guan-Ting Reservoir, Beijing, China, from 2004 to 2005. The phytoplankon community was composed of 8 divisions (94 species, including genus and varieties) and the average cell density was 980.93× 104 cells per liter. The dominant divisions were Chlorophyta (36.8%), Bacillariophyta (31.0%) and Cyanophyta (23.4%). The removal rate of phytoplankton density was 72.7%. There was a positive linear correlation between phytoplankon density and total phosphorus. Here, 7 families (13 species) of aquatic vasular plants were found, which constituted emerging and submerging macrophyte communities. In the wetland system, the zooplankton community consisted of Protozoa, Rotifera, Cladocera and Copepoda (70 species). The average density was 4883 individuals per liter. Protozoan and Rotifera were the dominant groups and the removal rate of their density was 81.9%. The correlation between zooplankton and phytoplankton presented a quadratic curve. Also, the zoobenthos community contained Olisochaeta, Uniramia, Crustacea and Mollusca (15 species). The average density was 5670 individuals per m2 (62.3% was Uniramia) and the removal rate of their density was 92.4 %. The wetland system reduced CODMn, BOD5, TN, NH3-N, NO3-N, TP (total phosphor), PO4-P and SS in the water of Yong Ding River at 52.9%–99.1%.    

3.  Biodiversity and water quality variations in constructed wetland of Yongding River system  
   Liu C  Du G S  Huang B B  Meng Q Y  Li H M  Wang Z J  Song F《生态学报(英文版)》,2007年第27卷第9期
   This research was carried on in constructed wetlands of Guan-Ting Reservoir, Beijing, China, from 2004 to 2005. The phytoplankon community was composed of 8 divisions (94 species, including genus and varieties) and the average cell density was 980.93× 104 cells per liter. The dominant divisions were Chlorophyta (36.8%), Bacillariophyta (31.0%) and Cyanophyta (23.4%). The removal rate of phytoplankton density was 72.7%. There was a positive linear correlation between phytoplankon density and total phosphorus. Here, 7 families (13 species) of aquatic vasular plants were found, which constituted emerging and submerging macrophyte communities. In the wetland system, the zooplankton community consisted of Protozoa, Rotifera, Cladocera and Copepoda (70 species). The average density was 4883 individuals per liter. Protozoan and Rotifera were the dominant groups and the removal rate of their density was 81.9%. The correlation between zooplankton and phytoplankton presented a quadratic curve. Also, the zoobenthos community contained Olisochaeta, Uniramia, Crustacea and Mollusca (15 species). The average density was 5670 individuals per m2 (62.3% was Uniramia) and the removal rate of their density was 92.4 %. The wetland system reduced CODMn, BOD5, TN, NH3-N, NO3-N, TP (total phosphor), PO4-P and SS in the water of Yong Ding River at 52.9%–99.1%.    

4.  氮素形态对树木养分吸收和生长的影响  被引次数:23
   张彦东  白尚斌《应用生态学报》,2003年第14卷第11期
   由于NH4+-N和NO3--N形态的差异,二者对树木养分吸收和生长发育的影响不同,树木常表现出对NH4+-N和NO3--N的选择性吸收,树种对NH4+-N和NO3--N吸收的偏好特性可能与生长地的土壤pH有关,来自于酸性土壤的树种通常具有喜NHON的特性,而来自于中性或碱性土壤的树种常表现出喜NO3--N的趋势,由于NH4+-N和NO3^--N所带电荷的差异,通常NH4+-N有利于阴离子的吸收,而NO3^--N则促进阳离子的吸收,在有些情况下,NH4+-N会抑制NO3--N的吸收,但抑制的机制目前还不清楚,树木吸收NH4+-N时,引起根际pH下降,相反吸收NO3--N时根际pH升高,根际pH变化可以改变土壤养分的有效性,并影响树木对养分的吸收利用,树木对NH4+-N和NO3--N的生长反应不同,有些喜NH4+-N的针叶树在供应NH4+-N时生长较好,多数植物在同时供应NH4+-N和NO3--N时生长量最大,有些树种在同时供应NH4+-N和NO3--N时也表现出最高的生长,但对于树木类似的研究还少,这一现象对于树木是否具有普遍性还需要大量试验证明。    

5.  不同浓度铵态氮对镉胁迫轮叶黑藻生长及抗氧化酶系统的影响  被引次数:3
   许秋瑾  金相灿  王兴民  扈学文  陈书琴  颜昌宙《应用生态学报》,2007年第18卷第2期
   用含有不同浓度NH4+-N (0、0.5、2.0和4.0 mg·L-1)和10 mg·L-1Cd的1/10 Hoagland营养液培养沉水植物轮叶黑藻,研究了氨态氮对Cd胁迫下轮叶黑藻的生长及抗氧化酶系统的影响,探讨富营养化污染水体沉水植物退化机理.结果表明,10 mg·L-1Cd对轮叶黑藻能产生明显的胁迫作用,叶绿素合成量明显降低;超氧化物岐化酶(SOD)与过氧化物酶(POD)活性呈先升高后下降的趋势.NH4+-N能加速Cd对植物的胁迫作用,单因子Cd作用3 h时SOD明显升高,而Cd和NH4+-N共同作用0.5 h SOD就明显升高.Cd与NH4+-N共同作用时,相对于叶绿素和蛋白质,抗氧化酶是早期敏感指标,并且SOD比POD更敏感.本试验条件下,NH4+-N与Cd共同作用2 d后,对轮叶黑藻的胁迫作用主要取决于Cd,NH4+-N的作用几乎可以忽略.    

6.  Investigation of bacterial community diversity in water of Zoige Alpine Wetland  
   Chen X  Feng S G  Zhang H X  Wang Y F  Bai Z H  Gu L K《生态学报(英文版)》,2011年第31卷第4期
   Spatial isolation is currently thought to represent one of the major factors resulting in bacteria genetic variation and population abundance. The bacterial diversity in a distinct environment Zoige Alpine Wetland located in the northeast of the Qinghai-Tibetan Plateau with the altitude 3400 m on average aroused our great attention. This area belongs to Qinghai-Tibetan cold climate zone with the mean annual temperature about 1 °C. Although several studies on bacterial diversity in Qinghai-Tibetan Plateau had been reported, there is no report on wetland water in this area. In this work, six water samples were collected and the water qualities including CODCr, NH4+-N, NO3--N, NO2--N, TN, TP, TOC were investigated, of which results indicated that more than 80% samples sorted as II–V class of surface water sources according to the National Water Quality Standard of China (GB3838-2002). Comparison of bacterial communities among the six samples was analyzed by DGGE of PCR-amplified 16S rDNA with universal bacterial primer sets. The profiles demonstrated that samples from the Flower Lake had more DNA bands than the Conservatory Station inferring higher diversity. In addition, the samples from the same environment shared similar compositions of bacterial communities. Bacterial community composition and predominant bacteria were analyzed by 16S rDNA clone library. The dominant group was Proteobacteria (51.6% of the total clones, which contained 24.2% alpha proteobacteria, 14.5% beta proteobacteria and 12.9% gamma proteobacteria). And the Bacteroidetes added to 17.7%, Verrucomicrobia to 4.8%. More than 24.2% of the total clones showed high similarity to uncultured bacteria. The above work provides some information on bacterial diversity for special site of spatial isolation.    

7.  Investigation of bacterial community diversity in water of Zoige Alpine Wetland  
   Chen Xi  Feng Shugeng  Zhang Hongxun  Wang Yanfen  Bai Zhihui  Gu Likun《生态学报》,2011年第31卷第4期
   Spatial isolation is currently thought to represent one of the major factors resulting in bacteria genetic variation and population abundance. The bacterial diversity in a distinct environment Zoige Alpine Wetland located in the northeast of the Qinghai-Tibetan Plateau with the altitude 3400 m on average aroused our great attention. This area belongs to Qinghai-Tibetan cold climate zone with the mean annual temperature about 1 °C. Although several studies on bacterial diversity in Qinghai-Tibetan Plateau had been reported, there is no report on wetland water in this area. In this work, six water samples were collected and the water qualities including CODCr, NH4+-N, NO3--N, NO2--N, TN, TP, TOC were investigated, of which results indicated that more than 80% samples sorted as II–V class of surface water sources according to the National Water Quality Standard of China (GB3838-2002). Comparison of bacterial communities among the six samples was analyzed by DGGE of PCR-amplified 16S rDNA with universal bacterial primer sets. The profiles demonstrated that samples from the Flower Lake had more DNA bands than the Conservatory Station inferring higher diversity. In addition, the samples from the same environment shared similar compositions of bacterial communities. Bacterial community composition and predominant bacteria were analyzed by 16S rDNA clone library. The dominant group was Proteobacteria (51.6% of the total clones, which contained 24.2% alpha proteobacteria, 14.5% beta proteobacteria and 12.9% gamma proteobacteria). And the Bacteroidetes added to 17.7%, Verrucomicrobia to 4.8%. More than 24.2% of the total clones showed high similarity to uncultured bacteria. The above work provides some information on bacterial diversity for special site of spatial isolation.    

8.  垃圾填埋场渗滤液灌溉及覆土土质对填埋场氧化亚氮释放的影响  被引次数:4
   何品晶  陈淼  张后虎  邵立明《应用生态学报》,2008年第19卷第7期
   采用静态箱法,现场监测黏土和砂土覆盖层生活垃圾填埋场N2O释放通量的春夏季节及昼夜变化,研究渗滤液灌溉、覆土土质对填埋场N2O释放的影响.结果表明:砂土和黏土覆盖层填埋场N2O夏季的释放通量均值分别为(242±576)和(591±767) μg N2ON·m-2·h-1,是春季[分别为(74.4±314)和(269±335) μg N2ON·m-2·h-1]的3.2(P>0.05)和2.2倍(P<0.05).渗滤液灌溉促进了砂土填埋场覆土N2O的释放,填埋场中灌溉区N2O的释放通量为无灌溉区的2倍(P>0.05).渗滤液灌溉的砂土覆盖层填埋场N2O春夏两季释放通量均值[(211±460) μg N2ON·m-2·h-1]仅为无渗滤液灌溉的黏土覆盖层填埋场[(430±605) μg N2ON·m-2·h-1]的1/2(P>0.05).无论渗滤液灌溉与否,选择贫瘠的砂性覆盖土均有助于减少生活垃圾填埋场N2O释放.    

9.  Effects of temperature on purification of eutrophic water by floating eco-island system  
   Hu M H  Yuan J H  Yang X E  He Z L《生态学报(英文版)》,2010年第30卷第6期
   The effects of temperature on pollutant removal of two plant species (Oenanthe javanica D.C. and Nasturtium officinale) were evaluated using simulated microcosms of the floating eco-island system (FEIS). Both the planted FEIS (P-FEIS) and the non-planted FEIS (NP-FEIS) dramatically decreased NH4–N concentration in the hypereutrophic water at low (10 °C), medium (22 °C), and high (35 °C) temperatures, and to a greater extent for the P-FEIS and at medium temperature. The NO2–N concentration was effectively decreased from 0.23 to 0.01 mg/L after 4 d treatment with the P-FEIS at all the three temperatures, but was slightly increased in the NP-FEIS at low temperature. The P-FEIS could decrease NO3–N concentration in the eutrophic water over 1–3 times depending on temperature, with greater decrease at high temperature. The remove of total P (TP) reached 78%, on average, with the FEIS treatment for 4 d at all temperatures, which was over three times greater than those with the NP-FEIS at low temperature. The removal rates of Chla, CODMn, and BOD5 by the P-FEIS from the hypereutrophic water were, on average, 70%, 85%, 83% at 22 °C and 35 °C, respectively, while over 1–2 times smaller at 10 °C. More effective removals of Chla, CODMn, and BOD5 (over 1–2 times) were noted with the P-FEIS than those with the NP-FEIS. N. officinale showed more efficiency in removing ammonium and TN at low temperature, and BOD5 at medium and high temperatures, as compared to O. javanica. Whereas O. javanica could more effectively decrease Chla at 22 °C and 35 °C and CODMn at 10 °C than N. officinale after 4 d treatment. Higher dissolved oxygen concentration and pH was found in the FEIS with N. officinale than that with O. javanica D.C. The results imply that plant eco-island system had remarkable purification ability to remove pollutants from hypereutrophic water, and mixed planting of O. javanica D.C. with N. officinale on the FEIS may enhance nutrient removal and water quality improvement of eutrophic water bodies, especially at low temperature season.    

10.  Effects of temperature on purification of eutrophic water by floating eco-island system  
   Mian-Hao Hu  Ju-Hong Yuan  Xiao-E. Yang  Zhen-Li He《生态学报》,2010年第30卷第6期
   The effects of temperature on pollutant removal of two plant species (Oenanthe javanica D.C. and Nasturtium officinale) were evaluated using simulated microcosms of the floating eco-island system (FEIS). Both the planted FEIS (P-FEIS) and the non-planted FEIS (NP-FEIS) dramatically decreased NH4–N concentration in the hypereutrophic water at low (10 °C), medium (22 °C), and high (35 °C) temperatures, and to a greater extent for the P-FEIS and at medium temperature. The NO2–N concentration was effectively decreased from 0.23 to 0.01 mg/L after 4 d treatment with the P-FEIS at all the three temperatures, but was slightly increased in the NP-FEIS at low temperature. The P-FEIS could decrease NO3–N concentration in the eutrophic water over 1–3 times depending on temperature, with greater decrease at high temperature. The remove of total P (TP) reached 78%, on average, with the FEIS treatment for 4 d at all temperatures, which was over three times greater than those with the NP-FEIS at low temperature. The removal rates of Chla, CODMn, and BOD5 by the P-FEIS from the hypereutrophic water were, on average, 70%, 85%, 83% at 22 °C and 35 °C, respectively, while over 1–2 times smaller at 10 °C. More effective removals of Chla, CODMn, and BOD5 (over 1–2 times) were noted with the P-FEIS than those with the NP-FEIS. N. officinale showed more efficiency in removing ammonium and TN at low temperature, and BOD5 at medium and high temperatures, as compared to O. javanica. Whereas O. javanica could more effectively decrease Chla at 22 °C and 35 °C and CODMn at 10 °C than N. officinale after 4 d treatment. Higher dissolved oxygen concentration and pH was found in the FEIS with N. officinale than that with O. javanica D.C. The results imply that plant eco-island system had remarkable purification ability to remove pollutants from hypereutrophic water, and mixed planting of O. javanica D.C. with N. officinale on the FEIS may enhance nutrient removal and water quality improvement of eutrophic water bodies, especially at low temperature season.    

11.  长江三角洲地区雨水中NH4+-N/NO3--N和δ15NH4+值的变化  被引次数:3
   谢迎新  张淑利  赵旭  熊正琴  邢光熹《应用生态学报》,2008年第19卷第9期
   2003年6月至2005年7月,利用自行设计的雨水收集器对位于长江三角洲地区的常熟、南京和杭州3个观测点进行了全年性雨水观测,分析了雨水中NH4+-N/NO3--N和铵态氮自然丰度(δ15NH4+)值的变化.结果表明:研究区3个观测点雨水中NH4+-N/NO3--N和δ15NH4+值均呈相似的季节性变化规律,两者的规律性变化在以田间农事耕作为主的常熟观测点尤其明显,而位于市区的南京观测点和位于城乡结合部的杭州观测点的规律性次之;雨水中NH4+-N/NO3--N的峰值出现在6月下旬到8月上旬,然后逐渐下降,冬季降到最低;雨水中δ15NH4+值在6月下旬到8月中旬为负值,在8月下旬到11月中下旬为正值,12月至翌年3月又变为负值,5至6月中旬又转变为正值.雨水中NH4+-N/NO3--N和δ15NH4+值的季节变化与不同作物生育期间氮肥的施用、当地气候的季节性变化以及其他NH3释放源的NH3挥发有关(人和动物排泄物、氮污染水体及有机氮源中的氨挥发),其对大气湿沉降中NH4+的来源、形态组成及陆地不同NH3排放源的强度具有明显的指示意义.    

12.  不同酸中和对垃圾渗滤液厌氧生物处理的影响*  
   方卫   许玫英   岑英华   李云路   孙国萍  《微生物学通报》,2006年第33卷第6期
   不同时间垃圾填埋场渗滤液用石灰絮凝、吹脱后分别经磷酸、盐酸、硫酸调pH到7,在35℃条件下进行厌氧处理。试验表明,经磷酸或盐酸中和的早期垃圾渗滤液易于厌氧生物处理,38d后其COD、BOD5、VFA(挥发性脂肪酸)都有大幅度的降低。盐酸中和的早期垃圾渗滤液其厌氧产甲烷性能良好;但磷酸中和的其产甲烷性能被完全抑制。硫酸中和的早期垃圾渗滤液在反应过程中产生大量的硫化物,最高浓度达到1,241mg/L,对厌氧处理产生了严重的抑制,但是在第38d硫化物浓度达到最高后抑制作用慢慢减弱,其COD    

13.  五年填埋龄准好氧和厌氧填埋体及陈垃圾的理化特性  
   张维  岳波  黄启飞  黄泽春  张增强  王琪  林晔  王进安《应用生态学报》,2010年第21卷第3期
   研究了5年填埋龄准好氧和厌氧填埋体的沉降量、温度和填埋气产生特性,并分析了填埋处理陈腐垃圾的理化性质.结果表明:5年填埋龄准好氧填埋体的表面沉降量和减容率均显著高于厌氧填埋体,同时渗滤液回灌比清水回灌更容易引起垃圾填埋体表面的不均匀沉降.厌氧填埋体的平均温度(25.6 ℃)略高于准好氧填埋体(24.8 ℃),但差异不显著.准好氧填埋体不同时期的O2浓度分别高于厌氧填埋体,而CH4浓度则显著低于厌氧填埋体.5年填埋龄准好氧和厌氧填埋体陈垃圾中厨余物和纸类等易降解有机物含量显著降低,塑料、玻璃、砖瓦和竹木等含量则升高.其中,陈垃圾中有机质与营养元素含量均高于典型南方土壤;除厌氧填埋体陈垃圾中Cr含量超标外,其余重金属含量均未超出《土壤环境质量标准(GB 15618—1995)》的三级标准要求.    

14.  利用水生植物原位修复污染水体  被引次数:30
   方云英  杨肖娥  常会庆  张培民《应用生态学报》,2008年第19卷第2期
   在实验围隔系统中,夏季利用凤眼莲、冬季利用耐寒型沉水植物伊乐藻等恢复水生生态系统,研究水生植物对水体氮、磷营养盐、透明度等理化性质的影响.结果表明:水生植物处理围区营养盐水平均显著低于围区对照和大湖水体.最初15 d,凤眼莲生长速度快,覆盖面积从100 m2增加到470 m2;44 d后,覆盖面积达到65%,处理围区的水质最佳,总氮(TN)、铵态氮(NH4+ -N)、亚硝态氮(NO2- -N)、高锰酸钾盐指数(CODMn)和叶绿素a浓度最低,透明度达到1.7~1.8 m(水底).10月份后,处理围区水体总磷(TP)维持在0.1 mg·L-1左右.处理围区透明度提高后,伊乐藻逐渐成为优势种(覆盖面积达到总水域的1/3),在净化水质、维持水质理化性质稳定和提高透明度方面作用显著.表明水生植被恢复可以有效降低水体营养盐,控制浮游植物增长,是改善富营养湖泊水质的重要措施.    

15.  葡萄糖存在下选择性抑菌剂对土壤氮素转化的影响  
   王鸽  何红波  张旭东  李建东  韩琳  汪景宽《应用生态学报》,2006年第17卷第5期
   采用2种氮源并分别加入选择性微生物抑制剂进行室内培养,通过测定样品中NH4+-N和NO3--N及土壤中氨基葡萄糖和胞壁酸含量,研究土壤微生物氮素固持时间特征及其相对贡献.结果表明,加入青链霉素明显地降低了NH4+-N的转化速率,且影响远大于真菌抑制剂放线菌酮;氨基葡萄糖和胞壁酸的相对比例急剧增加,而后趋于平衡;加入放线菌酮后NO3-N转化速率持续下降,氨基葡萄糖的合成受到抑制,但加入细菌抑制剂青链霉素对其转化无显著影响.培养初期,细菌在葡萄糖存在下能快速固持NH4+-N和NO3--N,并以NH4+-N为首选氮源;培养后期,氮转化主要为真菌所推动,且真菌对NO3--N的利用能力显著大于细菌.    

16.  根际不同供氮水平对紫青菜生长和营养品质的影响  
   刘爱荣  张远兵  黄守程  胡能兵  林平《热带亚热带植物学报》,2016年第24卷第1期
   为了解根际供氮对紫青菜(Brassica campestris ssp. chinensis var. communis)生长和营养品质的影响,用不同水平的NH4NO3处理,对紫青菜的生理特性和营养品质进行研究。结果表明,随着供氮水平的增加,紫青菜的鲜质量、干质量、根系活力和游离氨基酸含量均呈先上升后略下降趋势;硝酸还原酶(NR)活性、叶绿素含量、NO3-含量和可溶性蛋白质含量均呈上升趋势;DPPH·自由基清除率、超氧化物歧化酶(SOD)活性、过氧化物酶(POD)同工酶和过氧化氢酶(CAT)同工酶活性等均提高;而花青素苷相对含量、可溶性糖含量、维生素C (Vc)含量、总酚含量和FRAP值均呈下降趋势。根际NH4NO3为75~300 mg L-1时,紫青菜的鲜质量和干质量较低;NH4NO3达1200 mg L-1时,其鲜质量和干质量又低于600 mg L-1处理的,植株积累较多NO3-,导致营养品质下降,同时根际也积累NH4NO3。因此,600 mg L-1的根际NH4NO3是适宜的氮水平,其鲜质量和干质量均最高、营养均衡、抗氧化能力强。    

17.  Cr6+对菹草叶绿素荧光参数、抗氧化系统和超微结构的胁迫影响  被引次数:3
   陈辉  施国新  徐勤松  王红霞  杨海燕  潘秋红《植物研究》,2009年第29卷第5期
   研究了不同浓度的Cr6+(0、1、10、30、50 mg·L-1)对菹草叶片叶绿素、叶绿素荧光参数、抗氧化系统、可溶性蛋白含量、膜脂过氧化产物、可溶性糖含量、超氧阴离子(O2—·)产生以及细胞超微结构的胁迫影响。结果表明,随着Cr6+浓度的增加,总叶绿素、叶绿素a、叶绿素b和叶绿素a/b均呈下降趋势,Fo先升后降,Fv/FmFmFv/Fo均逐渐降低;可溶性蛋白和谷胱甘肽(GSH)含量先升后降,抗坏血酸(AsA)含量逐渐下降;超氧化物歧化酶(SOD)活性逐渐下降,而过氧化物酶(POD)、过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)和谷胱甘肽还原酶(GR)活性均呈先升后降趋势; O2—·产生速率、丙二醛(MDA)和可溶性糖含量均表现先降后升趋势;电镜观察发现:随着Cr6+浓度的增加,对细胞超微结构的损伤也加剧,表现为叶绿体膨胀,被膜破裂,类囊体片层解体;线粒体嵴数目减少,呈空泡状。可见,Cr6+破坏了菹草正常生理活动的结构基础,造成菹草生理功能紊乱。    

18.  Avocado shoot culture, plantlet development and net CO2 assimilation in an ambient and CO2 enhanced environment  
   Witjaksono  Bruce A. Schaffer  Angel M. Colls  Richard E. Litz  Pamela A. Moon《In vitro cellular & developmental biology. Plant》,1999年第35卷第3期
   Summary The proliferation and survival of avocado nodal cultures of juvenile origin were affected by the form and concentration of nitrogen. Optimum growth was achieved on modified Murashige and Skoog medium containing 67% KNO3 and 33% NH4NO3 with total N of 40 mM supplemented with 100 mg l−1 myo-inositol, 1 mg l−1 thiamine HCl, 30 g l−1 sucrose, and 4.44 μM BA with a 16-h photoperiod (120–150 μmol m−2 s−1). Proliferating shoots and plantlets were photosynthetically active. Better shoot growth and accumulation of higher biomass occurred in a CO2-enriched environment than under ambient CO2 conditions. CO2 assimilation efficiency, however, was higher under the latter conditions than in a CO2-enhanced environment, e.g., 31±7 and 17±2 μmol CO2 m−2 s−1, respectively. The net CO2 assimilation rates of in vitro grown plantlets were comparable to those of seedlings ex vitro.    

19.  孝顺竹愈伤组织增殖培养基优化研究  
   袁金玲  岳晋军  吴晓丽  张朵  郭广平  顾小平《植物研究》,2010年第30卷第5期
   为筛选适宜孝顺竹愈伤组织继代增殖培养基,控制褐变发生,提高再生体系效率,对培养基组成如5种基本培养基、6种有机添加物、7种糖类和5种大量元素等因子进行试验分析。结果表明:培养20 d,基本培养基以MS效果较好,愈伤组织增殖2.8倍,白至淡黄色,致密;有机添加物以1.0 g·L-1脯氨酸效果较显著,愈伤组织增殖3.64倍,淡黄色,致密均一;碳源以30 g·L-1麦芽糖效果较好,愈伤组织增殖2.96倍,白至淡黄色,致密。5种大量元素中NH4NO3对孝顺竹愈伤组织增殖的影响达到显著水平,以825 mg·L-1为较佳浓度,培养29 d愈伤组织增殖可达5倍以上,部分出现根分化;适宜孝顺竹愈伤组织培养的大量元素组合为:KNO3 475 mg·L-1+NH4NO3 825 mg·L-1+MgSO4·7H2O 185 mg·L-1+KH2PO4 340 mg·L-1+CaCl2·7H2O 440 mg·L-1    

20.  新型吡唑类化合物DL-1的硝化抑制效应初探  被引次数:13
   孙志梅  梁文举  武志杰  左秀锦  史云峰《应用生态学报》,2006年第17卷第2期
   以国内外应用较为广泛的硝化抑制剂双氰胺(DCD)为参比对象,采用室内培养方法,对新型吡唑类化合物DL-1的硝化抑制效应进行初步探讨.结果表明,DL-1对土壤中铵的氧化过程具有显著的抑制效应,前3周的硝化抑制率可达70%以上,且硝化抑制能力在第14天至28天最强.与等量DCD相比,施用量为(NH4)2SO4氮量1.0%的DL-1在14、21和28 d使土壤中的NO3--N含量分别下降 26.23%、33.27%和23.31%;与不加抑制剂的对照处理相比,土壤NO3--N含量则分别下降了71.12%、69.10%和55.14%.当DL-1用量为(NH4)2SO4氮量的2。0%时,土壤的硝化作用受到了更强烈的抑制,到培养第90天试验结束,土壤中的NO3--N含量始终维持在较低水平.    

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