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当绿豆幼苗在自然条件下生长到12d龄时,剪下第一对叶片,漂浮在水面,以蓝光荧光灯照射叶片,用白光和黑暗为对照。蓝光处理可延缓叶片叶绿素和蛋白质含量的降低,促进气孔的开放,维持SOD活性在较高的水平,从而延缓了质膜相对透性的增大。因此,我们认为,蓝光可以延缓绿豆幼苗离体叶片的衰老。 相似文献
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蓝光延缓绿豆离体叶片衰老的研究 总被引：3，自引：0，他引：3
当绿豆幼苗在自然条件下生长到12d龄时,剪下第一对叶片,漂浮在水面,以蓝光荧光灯照射叶片,用白光和黑暗为对照。蓝光处理可延缓叶片叶绿素和蛋白质含量的降低,促进气孔的开放．维持SOD活性在较高的水平．从而延缓了质膜相对透性的增大。因此,我们认为,蓝光可以延缓绿豆幼苗离体叶片的衰老。 相似文献
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以夏播绿豆品种‘冀绿2号’和‘泰来’为材料,研究其开花至成熟期间主茎开花节叶片的叶绿素含量、净光合速率、酶促防御系统的保护酶SOD、CAT、POD活性和MDA积累量的动态变化规律。结果表明,两品种各功能叶片的叶绿素含量、净光合速率、SOD、CAT活性均在其开花15 d后逐渐下降,但POD活性和MDA含量随着叶片的衰老而升高。虽然两品种叶片衰老的总体变化趋势一致,但衰老进程存在着显著差异。与‘泰来’绿豆相比,‘冀绿2号’各功能叶片衰老过程中SOD、CAT活性下降较慢,叶片功能期持续时间长,生育后期仍能保持相对较高的净光合速率,籽粒产量显著高于‘泰来’绿豆。综合分析表明,在绿豆开花结荚期间,有效控制或延缓开花节叶片的衰老进程,维持叶片生理功能,对籽粒产量形成具有重要作用。 相似文献
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以小麦(Triticum aestivum L.)离体叶片为材料,采用药理学实验和激光扫描共聚焦显微镜技术,对内源一氧化氮(NO)在光延缓离体小麦叶片衰老过程中的作用进行了研究.结果显示:光照处理的同时添加NO清除剂血红蛋白(Hb)或硝酸还原酶抑制剂钨酸钠(Na2WO4)后,光抑制离体小麦叶片叶绿素和可溶性蛋白含量降低及丙二醛累积的效应均显著减弱;光照处理下小麦叶片硝酸还原酶活性和内源NO水平均明显高于黑暗条件下,而Na2WO4处理不仅能抑制光诱导的硝酸还原酶活性提高,还与Hb处理一样能显著降低光下叶片内源NO水平.结果表明,硝酸还原酶途径来源的NO参与了光抑制离体小麦叶片衰老的过程. 相似文献
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植物叶片衰老及其延缓的分子途径 总被引：8，自引：0，他引：8
综述了植物叶片衰老过程中基因表达的上调和下调两种趋势,以及与转ｉｐｔ基因研究有关的问题：（１）ＣＴＫ／ＩＡＡ平衡;（２）形态发育;（３）抗衰老作用;（４）启动子选择等。 相似文献
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三唑酮对玫瑰切花衰老指标的影响(简报) 总被引：11，自引：0，他引：11
三唑酮处理的玫瑰切花ＭＤＡ含量降低,ＰＯＤ活性维持在较高水平,蛋白质含量提高,ＳＯＤ活性变化不大。表明三唑酮可以延缓脂膜过氧化,降低膜的透过性,具有延缓玫瑰切花衰老的作用。 相似文献
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Treatment of triadimefon on detached leaves of mung bean (Phaseolus radiatus L. ) seedlings increased the levels of chlorophyll and soluble proteins. Declined activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and ascorbate-peroxidase (AsA-POD) and contents of ascorbate (ASA) and glutathione (GSH) were observed during the senescence of detached young leaves. Triadimefon at concentration of 20 mg/L promoted the activities of POD, AsA-POD and levels of AsA and GSH, but had no effect on the activities of SOD and CAT. On the other hand, the levels of malondialdehyde (MDA) were increased and the increase of which was markedly negative correlated with the activities of POD, AsA-POD and with the contents of AsA and GSH during the senescence of leaves. MDA contents were decreased by triadimefon treatment. These resuits suggested that triadimefon retarded the senescence of leaves in mung bean seedlings in terms of enhancing the protective ability of plant tissues against membrane lipid peroxidation. 相似文献
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首次从常用食品绿豆（Phaseolus radiatus L．）中分离得到7个已知化合物,通过波谱解析等方法确定其结构为：7-甲氧基牡荆素（1）,硬脂酸（2）,1-硬脂酸甘油脂（3）,色氨酸（4）,尿嘧啶核苷（5）,β-谷甾醇（6）和胡萝卜苷（7）。 相似文献
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The relative levels of polyribosomes and total ribosomal materials, the rates of RNA synthesis and the contents of each RNA component were investigated in excised cotyledons of mung bean (Phaseolus radiatus L.) incubated with and without kinetin. 12 h incubation with 50 μmol/ L kinetin markedly increased the levels of polyribosomes and decreased the levels of monoribosome, especially of the ribosomal subunits. In addition, levels of total ribosomal materials (ribosomal subunit+monoribosome+polyribosome) were also increased in cotyledons incubated with kinetin. The kinetin-promoted polyribosome formation could be arrested by the RNA synthesis inhibitor-actinomycin D(ACTD). Kinetin incubation greatly enhanced RNA synthesis and increased that RNA conten. A marked increase was found in the amount of poly(A)+-mRNA, while the levels of other RNA components (25S, 18S rRNA, 4–5S RNA) were also increased to different extent. These results suggest that the promotion of polyribosome formation by kinetin depends upon the de novo synthesis of mRNAs, and the promotion of ribosome con, struction by kinetin may also be related to the synthesis of rRNAs. 相似文献
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研究了胞壁钙在红光抑制黄化绿豆（ＰｈａｓｅｏｌｕｓｒａｄｉａｔｕｓＬ．）下胚轴切段伸长生长中的作用。培养在有Ｃａ２＋介质中的切段胞壁钙含量比无Ｃａ２＋介质中的高３倍多,但不论介质中有无外源Ｃａ２＋,红光对下胚轴伸长的抑制程度都为２０％～２５％。乙醇双乙胺醚Ｎ,Ｎ,Ｎ′,Ｎ′四乙酸（ＥＧＴＡ）减少胞壁钙含量,相应地抵消红光对伸长的抑制;ｖｅｒａｐａｍｉｌ、Ｌａ３＋处理的切段胞壁钙含量与黑暗对照接近,但削弱红光的抑制作用;Ａ２３１８７减少胞壁钙,相应地抵消红光作用,甚至促进伸长生长。此外,氯丙嗪不影响胞壁钙含量,却阻止红光抑制伸长。表明红光无需外源Ｃａ２＋也能抑制切段伸长生长,但并非完全不需要Ｃａ２＋,可能胞壁自身的Ｃａ２＋基本能满足伸长生长所需。胞壁Ｃａ２＋的作用很复杂,它既可作为钙库起内流Ｃａ２＋信号的作用,也可在壁区起生长调节作用。 相似文献
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When the hypocotyl segments of Phaseolus radiatus L. were incubated in CaC12 (1 mmol/L) medium, the cell wall calcium was increased over threefold more than those incubated in a Ca2+ -free medium. However, red light inhibited elongation of the hypocotyl was 20% to 25% both in the medium with or without Ca2 + . The amount of calcium removed from the wall by ethylene glycol-bis (2-aminoethyl ether)-N, N, N', N'-tetraacetic acid (EGTA) ( 1 to 10 mmol/L) was 58.13 % to 75.33%, which offset the red light-inhibited elongation of the hypocotyl by 61.29% to 87.1%. Moreover, treatment with the channel blocker, verapamil ( 10 to 100 μ mol/L), wall calcium was the same as that of the darkness control, by which the red light-inhibited growth was also offset. La3 + ( 100 to 1 000 μmol/L) had no effect on wall calcium as compared to hypocotyl segments treated with red light alone, but eliminated the inhibitory effect of red light. Treatment with the calcium ionophore, A23187 (10 to 100 μmol/L), red light-inhibited elongation was abolished by 66.67% to 142.45% while wall calcium was reduced by 24.53% to 42.81%. In addition, calmodulin antagonist chlorpromazine (1 to 10 μmol/L) also counter acted the red light-induced elongation inhibition. These data indicated that exogenous Ca2+ was involved in the red light-inhibition effect, but that' did not mean that Ca2 + was not required. Perhaps Ca2 + in the wall itself was sufficient for red light-induced inhibition of hypocotyl elongation. The role of wall calcium might be quite complex, it not only acted as a signal of influx Ca2 + from the Ca2 + pool, but also played a regulatory role in the cell wall. 相似文献
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The structure of embryo sac before and after fertilization, embryo and endosperm development and transfer cell distribution in Phaseolus radiatus were investigated using light and transmission electron microscopy. The synergids with distinct filiform apparatus have a chalazal vacuole, numerous mitochondria and ribosomes. A cell wall exists only around the micropylar half of the synergids. The egg cell has a chalazally located nucleus, a large micropylar vacuole and several small vacuoles. Mitochondria and plasrids with starch grains are abundant. No cell wall is present at its chalazal end. There are no plasma membranes between the egg and central cell in several places. The zygote has a complete cell wall, abundant mitochondria and plastids containing starch grains. Both degenerated and persistent synergids migh.t serve as a nutrient supplement to proembryo. The wall ingrowths occur in the central cell, basal cell, inner integumentary cells, suspensor cells and endosperm cells. These transfer cells may contribute to embryo nutrition at different developmental stages of embryo. 相似文献
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2.个D和(认;促进了好工们已幼苗胚轴中肌醇磷脂降解。用技射性标记本,n.(法分离.发现2、4-D与＊A..均对nP和H厂降解具促进作用(降解过早:PIP,、PIP).;1时DA(1十平什高、但PI水平创t不大、PP:{.。使肌酸磷脂代谢受列一定抑＄JI.刊、Pm一,n厂水平均高于对照.仁D八＊水平则低于对外。IAA和 fh醇含量 jj低于对照。2.1-D和(M。在一定程度_L运转＊P.;。;对肌醇刚S代谢的抑制作用。 相似文献
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Effects of actinomycin-D (ACT), cycloheximide (CH), rifampicin (RIF) and chloramphenicol (CAP) on senescence of soybean leaf discs were investigated. All inhibitors tested are effective in retarding senescence of soybean leaf discs. However, CH is more effective than ACT, RIF and CAP, suggesting that activation of preexisting, latent metabolic systems present in the cytoplasm piays predominant role in the initiating of leaf senescence. However, the possibility that events taking place in the nucleus or chloroplast are essential for the initiation of leaf senescence cannot be excluded. 相似文献