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1.
测定了小叶锦鸡儿(Caragana microphylla Lam.)花粗提物和不同极性组分总黄酮含量和各项抗氧化能力指标。结果表明:小叶锦鸡儿花不同极性的黄酮组分的抗氧化活性有明显差别,其总抗氧化能力与溶剂极性之间有显著正相关性(P<0.05),但其它抗氧化活性与溶剂极性之间均无显著相关性(P>0.05)。同一极性黄酮组分在不同抗氧化体系中的抗氧化活性有较大差别。对DPPH.自由基的清除能力最强的为乙酸乙酯层(EC50=0.11 mg/mL),其次是二氯甲烷层(EC50=0.38 mg/mL)和乙醇粗提物(EC50=0.56 mg/mL)。总抗氧化能力(T-AOC)最强的为水层,在1 mg/mL时为1.482个T-AOC能力单位。清除羟自由基.OH能力最强的为水层,其次为正己烷层和乙醇粗提物,在3.57 mg/mL时清除率分别为86.23%、66.04%和64.63%。清除超氧阴离子自由基O2-.能力最强的为水层,其次为乙醇粗提物,在1 mg/mL时其清除率分别相当于0.15 mg/mL Vc清除率的336.17%和318.20%。可见,小叶锦鸡儿花的粗提物和不同极性黄酮组分具有较好的抗氧化活性,可用于提取、制备抗氧化特性和作用不同的天然抗氧化剂。  相似文献   

2.
周婧  黄丽云  李钢  徐静 《微生物学通报》2017,44(8):1958-1966
【目的】红树林内生真菌生长于特殊的生境,有些菌株能分泌出结构新颖和具有生物活性的物质。从红树林植物红茄苳(Rhizophora mucronata)中分离获得一株内生真菌HQD24,对其进行生物学鉴定和抗氧化活性评价。【方法】综合运用形态结构及ITS r DNA序列分析,HQD24被鉴定为曲霉属(Aspergillus)的烟曲霉(Aspergillus fumigatus)。采用抗氧化多活性模型,2-2′二苯基-1-三硝基苯肼(DPPH)自由基测定法、2′-联氨-双-3-乙基苯并噻唑啉-6-磺酸(ABTS)自由基测定法、超氧自由基测定法、还原Fe~(3+)能力、螯合Fe~(2+)能力对HQD24麦麸发酵提取物的石油醚萃取物、二氯甲烷萃取物、乙酸乙酯萃取物和正丁醇萃取物进行抗氧化活性评价。【结果】经抗氧化活性实验发现不同有机溶剂萃取成分都具有清除DPPH自由基、ABTS自由基、超氧自由基和还原Fe~(3+)、螯合Fe~(2+)的能力,尤其是清除ABTS自由基和超氧自由基,抗氧化活性的能力都随着浓度的增高而增强。当不同有机溶剂萃取粗提物浓度为1.5 g/L时,二氯甲烷萃取物清除ABTS自由基率为71.92%,正丁醇萃取物清除超氧自由基率为62.36%,综合评价,HQD24的发酵产物中具有抗氧化活性的物质主要分布于二氯甲烷萃取粗提物和正丁醇萃取粗提物,其次分布于乙酸乙酯萃取粗提物,石油醚萃取粗提物中抗氧化活性物质含量最少。【结论】研究结果预示红树林内生烟曲霉菌是产生抗氧化活性物质的重要资源,HQD24菌株可以作为进一步实验研究的对象。  相似文献   

3.
姜宁  刘洋  朱宴妍  王琦 《菌物研究》2014,(3):160-163
为探索黏菌的抗氧化活性,以总还原力、DPPH自由基清除率及O2自由基清除率为指标,首次对煤绒菌原质团和菌核甲醇提取物进行抗氧化活性研究。结果表明:煤绒菌原质团和菌核甲醇提取物均有较好的抗氧化活性,当质量浓度为0.8 mg/mL时,二者对O2自由基的清除能力最强,清除率分别为70.39%和86.83%;当质量浓度为0.4 mg/mL时,二者对DPPH自由基的清除能力最强,清除率分别为57.22%和62.91%,通过比较IC50值,菌核粗提物的抗氧化能力略优于原质团。  相似文献   

4.
甘草提取物的体外抗氧化活性   总被引:2,自引:1,他引:1  
为研究甘草提取物的体外抗氧化作用,采用60%乙醇回流提取甘草(LF),甘草粗提物(LF0)通过AB-8吸附树脂纯化制取甘草精提物(LF1),对LF0和LF1主要活性成分的含量进行测定。分别采用DPPH法、2-脱氧-D-核糖法和NBT-NADH-PMS体系、β-胡萝卜素-亚油酸体系测定LF0和LF1对1,1-二苯基-2-苦苯肼自由基(DPPH.)、羟自由基(OH.)、超氧阴离子自由基(.O-2)的清除能力以及LF0和LF1的总抗氧化能力;通过对LF0和LF1还原能力的测定分析LF0和LF1的抗氧化活性。研究结果显示,LF0和LF1能有效清除.O2-、DPPH.和OH.,具有良好的总抗氧化能力和还原能力,且LF1的作用比LF0好。  相似文献   

5.
南极地衣提取物抗氧化能力的初步研究   总被引:1,自引:0,他引:1       下载免费PDF全文
韩乐琳  魏江春 《菌物学报》2009,28(6):846-849
本文讨论4种南极地衣簇花石萝Usnea aurantiacoatra,南极石萝Usnea antarctica,粉球衣Sphaerophorus globosus及一种石蕊Cladonia sp.甲醇提取物的体外抗氧化能力;采用亚油酸氧化体系、二苯代苦味酰自由基体系、还原力实验对其抗氧化活性进行测定,并同维生素C、丁基甲氧基苯酚进行比较。结果显示供试南极地衣均具有较高的抗氧化活性,且呈剂量依赖关系;其中,簇花石萝的脂质过氧化抑制率最高,还原力最大;石蕊的二苯代苦味酰自由基清除能力最大。此外,从簇花石萝中提取的松萝酸具有一定的抗氧化活性,但明显低于簇花石萝粗提物。  相似文献   

6.
微生物源性抗氧化剂体外抗氧化能力的初步研究   总被引:4,自引:0,他引:4  
目的:研究微生物源性抗氧化剂的体外抗氧化能力.方法:在体外分别测定微生物源性抗氧化剂、α-生育酚(Vε)、抗坏血酸(VC)、L-硫辛酸、表没食子酸儿茶素的还原能力,羟自由基、超氧阴离子自由基和DPPH自由基清除能力及抗脂质过氧化能力,比较微生物源性抗氧化剂与其他抗氧化剂抗氧化能力.结果:微生物源性抗氧化剂有较强的抗氧化能力,体外清除羟自由基、超氧阴离子自由基、DPPH自由基能力的半数有效量(EC50)分别为184.5μg、48.7μg、66.1 μg.与常见抗氧化剂相比,微生物源性抗氧化剂对氧自由基及氮自由基都有较好的清除自由基作用.结论:微生物源性抗氧化剂体外抗氧化作用明显,有进一步开发的价值.  相似文献   

7.
探讨红景天苷和西藏红景天粗提物对延长线虫寿命的影响及其体外抗氧化活性的比较。以红景天苷、红景天粗提物为研究对象、以Vc为阳性对照,分别检测以上三种物质对自由基的清除作用。结果表明红景天能够显著延长秀丽隐杆线虫的寿命;在相同浓度下,红景天苷清除DPPH自由基的EC50约为1291.53μg/mL,红景天提取物溶液约为489.76μg/mL;红景天苷清除羟自由基的EC50约为1120.94μg/mL,红景天提取物约为1068.37μg/mL;红景天苷清除超氧阴离子自由基的EC50约为55.57μg/mL,红景天提取物溶液约为40.49μg/mL。中药红景天中含有显著延长线虫寿命的抗氧化物质,而这种成分并非是红景天苷。  相似文献   

8.
以从疏花水柏枝(Myricaria laxiflora)中分离的1株内生真菌QY-1为研究对象,为分析其体内及体外的抗氧化活性,对其发酵液及其提取物的活性进行了综合评价。对nr DNA内转录间隔区进行测序鉴定。分别采用总抗氧化力试剂盒、自由基清除试剂盒和还原力测定试剂盒评价发酵液及粗提物的体外抗氧化能力活性;对发酵液提取物及其主成分进行了大肠埃希菌、人神经母瘤细胞SH-SY5Y的体内抗氧化损伤保护作用分析。结果表明QY-1是1株球毛壳菌(Chaetomium globosum),其粗提物具有很高的抗氧化活性,总抗氧化活力、自由基清除率及总还原力均接近维生素C的50%,远高于国际报道水平。且其发酵液提取物有促进细胞增殖和抗氧化保护作用,显著提高大肠埃希菌细胞和神经细胞在H2O2胁迫下的存活率。其粗提物可保护神经细胞膜的完整性,有效减低乳酸脱氢酶(LDH)的渗漏率。从粗提物中分离到一种主成分SF2,可显著抑制凋亡蛋白Caspase 3和Caspase 9的活性。结果表明内生真菌球毛壳菌QY-1不管在体内还是体外都具备较强的抗氧化能力,是一种具有潜在开发价值的天然抗氧化剂资源。  相似文献   

9.
采用几种化学及生物学模型考察了半枝莲、白花蛇舌草及其药对提取物抗氧化及清除自由基活性.研究结果表明三种提取物中,半枝莲提取物还原能力、清除DPPH及·OH自由基能力最强,药对提取物螯合Fe2 能力以及保护DNA免受自由基损伤能力最强.提示提取物抗氧化及清除自由基活性可能与其黄酮组成及含量相关.推测药对的抗癌作用机理可能与金属离子的螯合能力和对DNA损伤的保护作用等有关.  相似文献   

10.
为研究21株蒺藜内生真菌的抗氧化活性及筛选出一株抗氧化活性较好的菌株,本实验首先以总抗氧化能力为指标评价PDB培养基和察氏培养基发酵条件下所有菌株的抗氧化活性,选出总抗氧化能力都较强的前9株菌株测定其对DPPH·自由基、羟自由基和超氧阴离子自由基的清除作用,发现JL13、JL14和JL17菌株发酵产物的清除效果最为明显,因此对三株菌株发酵产物乙酸乙酯萃取部位清除自由基的活性进一步评价,结果显示:JL13菌株发酵产物清除DPPH·自由基、羟自由基和超氧阴离子自由基的能力都是最强的,ECs0依次为149.67、439.91和514.77μg/mL.该结果表明,蒺藜内生真菌具有较好的抗氧化潜力,具有一定的开发利用价值,尤其是JL13菌株,可以作为进一步实验研究的对象.  相似文献   

11.
New resveratrol analogues containing five- and six-membered nitroxides and isoindoline nitroxides were synthesized. These new compounds were compared to resveratrol based on their ABTS radical scavenging ability as well on their capacity to suppress inflammatory process in macrophages induced by lipopolysaccharides. The ABTS and ROS scavenging activities of new molecules were the same or weaker than that of resveratrol, but some of paramagnetic resveratrol derivatives suppressed nitrite and TNFα production more efficiently than resveratrol. Based on these results the new nitroxide and phenol containing hybrid molecules can be considered as new antioxidant and anti-inflammatory agents.  相似文献   

12.

Melinjo seed extract contains melinjo resveratrol compounds that has antioxidant activity. The radical-scavenging sites required for the antioxidant activity, however, is yet to be located. We report a computational study that aims to locate scavenging sites of the simplest resveratrol dimer, gnetin C. We consider the reaction of gnetin C and hydroperoxyl radical energetically with the basis of density-functional calculations, to be compared with the reaction of the resveratrol monomer, trans-resveratrol, and hydroperoxyl radical. The results show that OH group at the para position is the most reactive scavenging site for both molecules. Besides the OH group, gnetin C also provides two CH groups in the furan ring that are favorable as scavenging sites. Therefore, furan ring plays an important role in the scavenging activity, which is contrary to the experimental speculation that propose resorcinol ring. Our study shows the prospect of density-functional calculation for studying the radical-scavenging reaction.

  相似文献   

13.
Resveratrol (RSV) analogues have attracted much attention because of the expected health functions including antioxidant activities. We have carried out a quantitative determination of the scavenging abilities of six trans-RSV analogues against various reactive oxygen species and methyl radical (hydroxyl radical, superoxide, alkoxyl radical, peroxyl radical, methyl radical, and singlet oxygen). RSV analogues are in general more potent scavenger than the parent RSV. Furthermore, piceatannol (PIC) having two OH groups in the ortho position of resveratrol was found to show 11 times higher scavenging ability against peroxyl radical than parent resveratrol. With the aid of previous theoretical studies, the enhanced antioxidant ability was interpreted based on the effects of substituent that modifies the original resveratrol structure and function.  相似文献   

14.
Stable free radical scavenging and antiperoxidative activities of resveratrol, a component of grapes and red wine, were evaluated and compared with some other known bioflavonoids (quercetin, catechin, kaempferol, myricetin, fisetin, ellagic acid and naringenin) widely present in the plant kingdom. Free radical scavenging activity was measured in an in vitro chemical system (DPPH assay), while for antiperoxidative activity, biological system comprising of hepatic and pulmonary homogenates was employed. Antiradical activity assay showed quercetin and myricetin to be stronger antiradical agents than resveratrol. Structure-activity study revealed that O-dihydroxy group on ring B of flavonoid plays a crucial role. A double bond at 2-3 position conjugated with a 4-oxo function and hydroxy groups at positions 3 and 5 also contribute towards antiradical activity of flavonoids. Resveratrol exhibited stronger antiradical activity than kaempferol and naringenin and was also more efficient than alpha-tocopherol, a known strong endogenous non-flavonoid antioxidant, used for comparison. In vitro antiperoxidative assay showed fisetin as the strongest and kaempferol as the weakest antioxidant. Resveratrol was found to be stronger antioxidant than catechin, myricetin, kaempferol and naringenin, but was weaker than quercetin, fisetin and alpha-tocopherol. Antiradical and antiperoxidative activities of resveratrol may explain its beneficial effects in disease states. Assays exhibited no direct correlation between antiradical and antiperoxidative activities of the phenolics.  相似文献   

15.
目的从鄂尔多斯婴儿粪便中分离出动物双歧杆菌,并对其进行抗氧化性的研究。方法结合细菌形态学和16SrDNA序列同源性分析进行鉴定,并对其还原性和·OH自南基清除能力进行了抗氧化研究。结果该分离出的菌株为动物双歧杆菌,其还原能力与·OH自由基清除能力随着浓度的增加而增大。结论动物双歧杆菌具有抗氧化性且与菌液的浓度呈现效量关系。  相似文献   

16.
The aim was to study the antioxidant properties of four wine polyphenols (flavonoids catechin, epicatechin, and quercetin, and hydroxystilbene resveratrol). All three flavonoids exerted significant and dose-dependent scavenging effects against peroxyl radical and nitric oxide in chemical systems. The scavenging effect of resveratrol was significantly lower. All polyphenols decreased production of reactive oxygen species (ROS) by RAW264.7 macrophages. Only quercetin quenched ROS produced by lipopolysaccharide-stimulated RAW264.7 macrophages incubated for 24 h with polyphenols. Quercetin and resveratrol decreased the release of nitric oxide by these cells in a dose-dependent manner which corresponded to a decrease in iNOS expression in the case of quercetin. In conclusion, the higher number of hydroxyl substituents is an important structural feature of flavonoids in respect to their scavenging activity against ROS and nitric oxide, while C-2,3 double bond (present in quercetin and resveratrol) might be important for inhibition of ROS and nitric oxide production by RAW 264.7 macrophages.  相似文献   

17.
印度块菌提取物抗氧化活性的研究   总被引:4,自引:0,他引:4  
郭坦  侯成林  魏磊  孙娟  范黎 《菌物学报》2010,29(4):569-575
对印度块菌Tuber indicum子实体的提取物,包括55%乙醇粗提物(ECE)、石油醚提取物(PEF)和乙酸乙酯提取物(EAF)的清除DPPH自由基和羟基自由基能力、铁离子鳌合能力以及各提取物的总酚含量等进行了研究和测定,结果显示,3种提取物的清除自由基能力和铁离子鳌合能力具有显著差异(P0.05);ECE对DPPH自由基的清除活性最高,其EC50值为1.61g/L;EAF对羟基自由基及铁离子表现出较强的清除或螯合的能力,其EC50值分别为3.31g/L和0.70g/L;EAF的总酚含量(2.964mg GAE/g提取物)最高,其次是ECE,总酚含量为(2.618mg GAE/g提取物);PEF的清除自由基和铁离子鳌合能力较差,其总酚含量也最低(1.124mg GAE/g提取物);总酚含量与印度块菌提取物清除自由基以及鳌合铁离子的能力密切相关。  相似文献   

18.
The extracellular polysaccharide from Rhodella reticulata was separated from the culture medium followed by concentration and ethanol precipitation, and purified by anion exchange chromatography on DEAE-Sepharose Fast Flow. This study compared the free radical-scavenging property and antioxidant activity with various treatments of crude extracellular polysaccharides of R. reticulata. The results showed that both the crude extracellular polysaccharide and deproteinized crude extracellular polysaccharide gave evidence of the free radical scavenging and antioxidant activity in a dose-dependent manner. The crude extracellular polysaccharide exhibited higher free radical scavenging capacity and better antioxidant activity than the various treatments of crude extracellular polysaccharide samples. The superoxide anion radical scavenging ability of various samples was significantly higher compared to standard antioxidant (α-tocopherol). These results indicate that the extracellular polysaccharide of R. reticulata is a potent natural antioxidant.  相似文献   

19.
以野生百合渥丹、山丹和传统食用的兰州百合为研究对象,对其鳞茎中多酚类物质、11种单体酚的含量及抗氧化活性(ABTS自由基、超氧阴离子、羟自由基的清除能力,铜离子还原能力以及抑制脂质过氧化活性)进行了分析。结果表明:两种野生百合鳞茎中的多酚类物质含量及抗氧化活性均显著高于兰州百合。3种百合鳞茎中单体酚的种类也有所不同,但均含有没食子酸、矢车菊素-3-芸香糖苷、儿茶素、表儿茶素、杨梅酮、芦丁、对香豆酸、山奈酚。相关性分析显示,除对羟自由基的清除力外,各酚类物质总量与不同抗氧化指标之间呈显著正相关关系。试验结果认为,野生百合鳞茎可作为天然抗氧化资源应用于食品和医药业,具有一定的开发应用前景。  相似文献   

20.
In this study, di(2,6-dimethylphenol) (Di-DMP), di(2,6-diisopropylphenol) (Di-DIP, dipropofol) and di(2,6-di-t-butylphenol) (Di-DTP) were synthesized by the reaction of monomeric phenol derivatives with catalytic CuCl(OH). TMEDA and Na2S2O4. Their antioxidant capacity and radical scavenging activity were examined using different in vitro methodologies such as 1,1-diphenyl-2-picryl-hydrazyl (DPPH*) free radical scavenging, 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical scavenging activity, total antioxidant activity by ferric thiocyanate, total reducing power by potassium ferricyanide reduction method, superoxide anion radical scavenging, hydrogen peroxide scavenging and ferrous ions chelating activities.  相似文献   

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