天敌瓢虫种群遗传学及其在生物防治中的应用

天敌瓢虫常在农业生产的生物防治中被广泛引进和使用。这种使用过程往往会导致天敌瓢虫在遗传水平的改变,并最终影响到其生物防治的使用效果和本地生态多样性。对天敌瓢虫种群遗传学的研究,有助于我们认识甚至预测这种遗传改变的规律和生态机制,从而为生物防治计划的优化提供理论基础。本文介绍了种群遗传学的研究方法,分析在生物防治中促进天敌瓢虫遗传改变的因素,以及几种常见的天敌瓢虫种群演变情况,并讨论种群遗传学在天敌瓢虫研究的未来方向。     

朱砂叶螨优势种天敌昆虫的评价

对绣球花Hydrangea macrophylla、茄子Solanum melongena和玉米Zea mays 3种植物上朱砂叶螨Tetranychus cinnabarinus天敌昆虫种类及发生数量进行调查,并采用优势度指数评价其优势种,结果表明,3种寄主植物上天敌的种类及其优势种有所不同,绣球花上有深点食螨瓢虫Stethorus punctillum、塔六点蓟马Scolothrips takahashii、食螨瘿蚊Acaroletes sp.和食螨隐翅虫Oligota sp.4种天敌,塔六点蓟马和深点食螨瓢虫为优势种;茄子上有塔六点蓟马、微小花蝽Orius minutus、食螨瘿蚊和深点食螨瓢虫4种天敌昆虫,塔六点蓟马为优势种;玉米上有深点食螨瓢虫、塔六点蓟马和食螨瘿蚊3种天敌昆虫,深点食螨瓢虫为优势种.通过测定各种天敌成虫和幼虫对朱砂叶螨成螨、若螨、幼螨和卵的日捕食量,并引入相对差距和法评价优势种天敌昆虫,结果表明,深点食螨瓢虫和微小花蝽是朱砂叶螨的优势种天敌.     

"518"油桃主要害虫与其捕食性天敌的关系

为了科学施药,合理保护和利用天敌进行油桃园害虫的综合防治,用t检验法比较 “518”油桃季节间和年度间害虫及其天敌的种群差异,结果表明两年春-夏季之间小绿叶蝉、桃蚜、山楂叶螨和李肖叶甲4种害虫的t值分别为0.8049、1.1449、0.0700和0.9895,t值均小于t0.05（2.26）,差异均不显著;异色瓢虫、龟纹瓢虫、粽管巢蛛、八斑球腹蛛、三突花蟹蛛、草间小黑蛛、中华草蛉和黑带食蚜蝇8种天敌的t值分别为1.7014、0.9098、1.4304、0.6239、0.1545、0.0544、1.5579和0.8249,t值均小于t0.05（2.26）,差异均不显著。2009年春-夏与2009年秋季之间,桃蚜的t值为38.8150,粽管巢蛛的t值为9.1706,t>t0.01（3.355）,差异均极显著,异色瓢虫、三突花蟹蛛、黑带食蚜蝇的t值分别为2.3332,3.1497和2.4712,t>t0.05（2.306）,差异显著;2010年春-夏与2009年秋季之间,粽管巢蛛t值为7.0763,t>t0.01（3.355）,差异极显著,其余差异均不显著。采用灰色系统分析方法、生态位分析方法,对2009年及2010年春-夏季油桃园桃蚜、小绿叶蝉、山楂叶螨和李肖叶甲与其捕食性天敌在数量、时间和空间格局等方面关系进行分析,对每一种天敌对应 的关联度、生态位重叠指数和相似性比例等参数标准化后相加,按照参数标准化值之和大小排序得出,2009年春-夏季小绿叶蝉前三位的主要天敌是异色瓢虫、八斑球腹蛛和龟纹瓢虫;桃蚜的主要天敌是中华草蛉、粽管巢蛛和异色瓢虫;山楂叶螨的主要天敌是草间小黑蛛、三突花蟹蛛和龟纹瓢虫;李肖叶甲的主要天敌是草间小黑蛛、八斑球腹蛛和龟纹瓢虫。2010年春-夏季小绿叶蝉的主要天敌是龟纹瓢虫、八斑球腹蛛和草间小黑蛛;桃蚜的主要天敌是中华草蛉、黑带食蚜蝇和粽管巢蛛;山楂叶螨的主要天敌是八斑球腹蛛、草间小黑蛛和龟纹瓢虫;李肖叶甲的主要天敌是草间小黑蛛、八斑球腹蛛和异色瓢虫。2009年秋季小绿叶蝉的主要天敌是龟纹瓢虫、草间小黑蛛和异色瓢虫;桃蚜的主要天敌是八斑球腹蛛、异色瓢虫和粽管巢蛛;山楂叶螨的主要天敌是三突花蟹蛛、龟纹瓢虫和八斑球腹蛛;李肖叶甲的主要天敌是草间小黑蛛、八斑球腹蛛和三突花蟹蛛。4种害虫种群聚集均数λ均大于2,其聚集是害虫本身原因造成的,2009年5月5日的天敌异色瓢虫、中华草蛉和6月18日的天敌草间小黑蛛 值均大于2,聚集是由于天敌本身原因所致。三个时间段其余天敌的 值均小于2,表明其聚集时由环境中某一因子影响所致。     

东北地区大豆田天敌昆虫优势种类分析

2005~2007年,对中国哈尔滨地区大豆田天敌昆虫开展系统调查。共发现5目、12科、33种天敌昆虫。龟纹瓢虫Propylaea japonica(Thunberg)、异色瓢虫Harmonia axyridis(Pallas)、隐翅甲Ischnosoma sp.成虫和花蝽Oriussp.为大豆田优势种天敌昆虫。其中,隐翅甲Ischnosomasp.成虫于6月上旬至下旬田间发生。花蝽Oriussp.成虫6月下旬至8月下旬发生,异色瓢虫成虫6月下旬至9月下旬发生。龟纹瓢虫幼虫、异色瓢虫幼虫和花蝽Oriussp.若虫多于6月下旬至8月下旬,即大豆生育中期发生;龟纹瓢虫成虫田间发生期较长,大豆生育期中6月中旬至9月下旬均有发生。     

转cry1Ab基因玉米对瓢虫科天敌种群动态的影响

【背景】瓢虫科天敌昆虫是玉米田中重要的天敌类群,可捕食多种农业害虫,对害虫有很好的控制作用。为了明确转基因玉米对瓢虫科天敌的潜在效应。【方法】在田间条件下,采用系统调查结合吸虫器方法,研究了cry1Ab基因玉米对玉米田主要瓢虫科天敌种类丰富度和种群动态的影响。【结果】转cry1Ab基因玉米田与其对照田瓢虫类群和优势种群组成相同。春播时Bt玉米田与对照田以龟纹瓢虫为优势种,仅在玉米生长后期Bt玉米田中的龟纹瓢虫数量显著高于对照田,其他时期2种田间龟纹瓢虫的种群动态没有显著差异。夏播玉米田以龟纹瓢虫和异色瓢虫为主,在8月17日Bt玉米田中异色瓢虫数量显著高于对照田,在9月27日其异色瓢虫数量显著低于对照田,而其他时期Bt玉米田和对照田中异色瓢虫优势度以及田间种群动态没有显著差异。【结论与意义】转cry1Ab基因玉米对玉米田重要的瓢虫科天敌丰富度、优势度和种群数量没有不良影响。因此,该转基因玉米可用于农田生态系统的有害生物管理。     

枇杷园害虫天敌资源调查初报

根据田间系统调查,共发现枇杷园天敌99种,隶属于2个纲6个目23个科73个属;另发现1种寄生性病原微生物.其中龟纹瓢虫、红点唇瓢虫、六斑月瓢虫、黑带食蚜蝇和猫蛛、跳蛛是主要的捕食性天敌.     

美国杂交杏李花果期节肢动物的生态位分析

美国杏李园害虫中绣线菊蚜与山楂叶螨的生态位宽度较宽,占据空间较大,为害虫优势种群,在空间维度上害虫之间存在较大竞争;大草蛉在各期的生态位宽度最大,说明大草蛉是重要的优势天敌,且天敌与主要害虫在空间上相遇机率较大;天敌之间生态位重叠指数较高,表明主要天敌之间存在明显的相互竞争关系;美国杏李园天敌对主要害虫在时间维度上有较强的追随效应和控制作用.主要天敌的数量与主要害虫绣线菊蚜种群数量追随关联度顺序为草蛉类>龟纹瓢虫>蜘蛛类>异色瓢虫>其它瓢虫类>食蚜蝇类>七星瓢虫>多异瓢虫.草蛉类和龟纹瓢虫与绣线菊蚜的模糊贴近度最高,说明这两种天敌与绣线菊蚜的同域效应高,对绣线菊蚜有较强的追随作用,抑制着绣线菊蚜种群数量的增长.     

天敌对麦长管蚜和麦二叉蚜种群数量影响程度的分析

通过对１９９３～１９９６年两种麦蚜种群数量及其天敌数量的系统调查,并采用灰色关联分析法,研究各种天敌对两种麦蚜种群数量的影响程度,得出对麦长管蚜种群数量影响最大的是龟纹瓢虫、蚜茧蜂和食蚜蝇;对麦二叉蚜种群数量影响最大的是草间小黑蛛,其次是龟纹瓢虫．     

桃园山楂叶螨、桃一点斑叶蝉及其主要天敌生态位

对山东桃园山楂叶螨、桃一点斑叶蝉及其主要天敌的时间、空间和时间一空间二维生态位宽度和生态位重叠进行了研究。结果表明,主要天敌异色瓢虫、七星瓢虫、龟纹瓢虫、大草蛉和蜘蛛与该2种害虫在时间上的同步性和空间上的同域性均较强,为保护利用天敌控制该2种害虫提供了依据。     

海南北部蔬菜生境天敌瓢虫多样性研究

为了解海南北部主要蔬菜作物生境天敌瓢虫的群落结构和物种多样性，本文利用黄板诱集法于2019年对茄子、豇豆和辣椒生境的天敌瓢虫种类和数量进行了调查研究。结果表明，3种作物生境共采集获得瓢虫标本4 993头，隶属于16个属28种，其群落多样性从高到低依次为茄子、辣椒和豇豆生境，其中茄子生境拥有28种，辣椒和豇豆生境分别为10种和11种。茄子生境的多样性指数、丰富度指数、优势度指数和均匀度指数均为最高，分别为1.58、3.40、0.27和0.47，明显高于豇豆和辣椒生境。无论哪种生境，六斑月瓢虫Cheilomenes sexmaculata (Fabricius)和龟纹瓢虫Propylae japonica (Thunberg)均是优势种类，分别占整个瓢虫数量相对多度的45.26%和19.67%。两种优势瓢虫时序变化特征表明，瓢虫数量呈多峰型分布，5月中下旬至7月上旬这一时段总体发生数量较低。研究结果可为海南北部蔬菜作物生境天敌瓢虫保护和利用提供理论依据。     

Crystal structure, detonation performance, and thermal stability of a new polynitro cage compound: 2, 4, 6, 8, 10, 12, 13, 14, 15-nonanitro-2, 4, 6, 8, 10, 12, 13, 14, 15-nonaazaheptacyclo [5.5.1.13, 11. 15, 9] pentadecane

A new polynitro cage compound 2, 4, 6, 8, 10, 12, 13, 14, 15-nonanitro-2, 4, 6, 8, 10, 12, 13, 14, 15-nonaazaheptcyclo [5.5.1.1(3,11).1(5,9)] pentadecane (NNNAHP) was designed in the present work. Its molecular structure was optimized at the B3LYP/6-31 G(d,p) level of density functional theory (DFT) and crystal structure was predicted using the Compass and Dreiding force fields and refined by DFT GGA-RPBE method. The obtained crystal structure of NNNAHP belongs to the P-1 space group and the lattice parameters are a = 9.99 ?, b = 10.78 ?, c = 9.99 ?, α = 90.01°, β = 120.01°, γ = 90.00°, and Z = 2, respectively. Based on the optimized crystal structure, the band gap, density of state, thermodynamic properties, infrared spectrum, strain energy, detonation characteristics, and thermal stability were predicted. Calculation results show that NNNAHP has detonation properties close to those of CL-20 and is a high energy density compound with moderate stability.     

Relations of the Al,B, Ba,Br, Ca,Cl, Cu,Fe, K,Li, Mg,Mn, Na,P, S,Si, Sr,and Zn mass fractions to morphometric parameters in pediatric and nonhyperplastic young adult prostate glands

The variation with age of the 18 trace element mass fractions and some histological characteristics of intact prostate glands of 50 subjects aged 0–30 years was investigated by instrumental neutron activation analysis, inductively coupled plasma atomic emission spectrometry, and a quantitative morphometric analysis. Mean values ± standard error of the mean (M ± SΕΜ) for the mass fractions (in milligrams per kilogram wet tissue) of these trace elements in pre-puberty were: Al 28.5 ± 9.0, B 0.40 ± 0.11, Ba 1.48 ± 0.44, Br 10.5 ± 1.5, Ca 241 ± 30, Cl 3,203 ± 278, Cu 3.51 ± 0.89, Fe 33.7 ± 4.1, K 2,364 ± 145, Li 0.020 ± 0.004, Mg 153 ± 23, Mn 0.46 ± 0.06, Na 2,286 ± 130, P 1,391 ± 100, S 1,698 ± 132, Si 62 ± 11, Sr 0.38 ± 0.08, and Zn 27.6 ± 2.3. During puberty and postpuberty, when there is a significant increase in circulating androgens, the mean values were: Al 7.2 ± 1.4, B 0.21 ± 0.05, Ba 0.25 ± 0.06, Br 5.8 ± 1.0, Ca 433 ± 81, Cl 2,314 ± 201, Cu 1.77 ± 0.13, Fe 20.9 ± 1.6, K 2,585 ± 118, Li 0.0088 ± 0.0014, Mg 232 ± 27, Mn 0.34 ± 0.04, Na 1,875 ± 107, P 1,403 ± 98, S 1,673 ± 73, Si 22.2 ± 3.1, Sr 0.22 ± 0.03, and Zn 93.3 ± 8.9. Mean values (M ± SΕΜ) of percent volumes (%) of the stroma, epithelium and lumen in the prostate before puberty were 73.4 ± 2.6, 20.4 ± 1.7, and 4.45 ± 0.94, respectively, versus 46.5 ± 2.5, 38.5 ± 1.9, and 14.9 ± 1.2 during puberty and postpuberty. This work’s results confirm that the Zn mass fraction in prostate tissue is an androgen-dependent parameter. For the first time it has been demonstrated that the glandular lumen is a main pool of Ca, Mg, and Zn accumulation and that the stroma is a main pool of Al, B, Ba, Br, Cl, Cu, Fe, Mn, Na, and Si accumulation in the normal human prostate, for the age range 0–30 years. It was concluded that the Ca, Mg, and Zn binds tightly within the prostatic fluid, because the volume of glandular lumen reflects the volume of prostatic fluid.     

The Effect of Age and Gender on Al, B, Ba, Ca, Cu, Fe, K, Li, Mg, Mn, Na, P, S, Sr, V, and Zn Contents in Rib Bone of Healthy Humans

The effect of age and gender on major, minor, and trace element contents in the intact rib bone of 80 relatively healthy 15–55-year-old women and men was investigated. Contents or upper limit of contents of 16 chemical elements in the rib bone were determined by inductively coupled plasma atomic emission spectrometry (ICP-AES). Mean values (M?±?SΕΜ) for the mass fraction of Ba, Ca, Cu, Fe, K, Li, Mg, Na, P, S, Sr, and Zn (milligram per kilogram of dry bone) were as follows: 2.54?±?0.16, 171,400?±?4,050, 1.35?±?0.22, 140?±?11, 1,874?±?71, 0.049?±?0.011, 2,139?±?38, 5,378?±?88, 75,140?±?1,660, 1,881?±?51, 291?±?20, and 92.8?±?1.5, respectively. The upper limits of contents of Al, B, Mn, and V were <7.20, <0.65, <0.36, and <0.03, respectively. Statistically significant tendency for the Ca, Mg, and P content to decrease with age was found in the human rib bone, regardless of gender. The mass fraction of Fe in the male rib bone increases with age. It was shown that higher Ca, Mg, Na, P, and Sr mass fractions as well as lower Fe content were typical of female ribs as compared to those in male ribs.     

Simultaneous measurement of the trace elements Al, As, B, Be, Cd, Co, Cu, Fe, Li, Mn, Mo, Ni, Rb, Se, Sr, and Zn in human serum and their reference ranges by ICP-MS

The goal of this article was to establish reference ranges of the concentration of trace elements in human serum and to compare these results with those reported by other authors. We describe the sample preparation and measurement conditions that allow the rapid, precise, and accurate determination of Al, As, B, Be, Cd, Co, Cu, Fe, Li, Mn, Mo, Ni, Rb, Se, Sr, and Zn in human serum samples (n=110) by inductively coupled plasma-mass spectrometry (ICP-MS). Accuracy and precision were determined by analyzing three reconstituted reference serum samples by comparison with other methods and by the standard addition procedure. The advantages of the ICP-MS method include short time of analysis of the elements mentioned, low detection limit, high precision, and high accuracy. Disadventages include a high risk of contamination due to the presence of some of the elements of interest in the environment, the relatively delicate sample handling, and the high cost of the equipment.     

Distribution of N,P, K,Ca, Mg,S, Zn,Fe, Mn and Cu in groundnut

Summary Concentration of N, P, K, Ca, Mg and S in summer groundnut crop was higher than in kharif while Zn, Fe, Mn and Cu contents were higher in summer crop. Kernel's N, P and Zn; Leaflet's Ca and Mn; Stem's K and Fe; Root's S and Cu and Petiole's Mg contents were highest. Shell's N, P, K, Mg, S, Zn and Cu; Kernel's Ca, Fe and Mn contents were the least. N, P, K, S, Zn and Cu concentrations decreased linearly as the crop grew. Ca, Mg, Fe and Mn concentrations did not display any distinct pattern. Ca concentration was positively correlated with pod yield in both the seasons.     

Reference values for the concentrations of As,Cd, Co,Cr, Cu,Fe, I,Hg, Mn,Mo, Ni,Pb, Se,and Zn in selected human tissues and body fluids

This report attempts to formulate reference ranges of elemental concentrations for 15 trace elements in selected human tissues and body fluids. A set of samples consisting of whole blood, blood serum, urine, milk, liver, and hair were chosen and considered for 15 elements of biological significance: As, Cd, Co, Cr, Cu, F, Fe, I, Hg, Mn, Mo, Ni, Pb, Se, and Zn. The results represent wholly or partially data received from 40 countries of the global regions of Africa, Asia, Europe, North, South, and Central America, Australia, and New Zealand. This survey, even if qualitative, has been useful in demonstrating certain trends of trace-element scenarios around the world. It is of course recognized that both diet and environment exert a strong influence on the distribution pattern of several elements, such as As, Cd, Mn, Pb, Se, and Zn. A limited comparison of the available information on soil status of different countries reflected some interesting associations for elements, such as Mn and Zn. Importantly, this study revealed that only a few countries were in a position to identify a reasonable amount of data on samples requested for this project. Regretably, for a number of countries, any dependable data for even such essential elements as Cu, Fe, and Zn were not available. In view of the nutritional importance of many elements, the time is ripe for concerted efforts by intergovernmental agencies to initiate investigations or commission task forces/projects to generate reliable reference data for selected global regions, which sadly lack data of any kind at present.     

Potential Compounds for Oral Cancer Treatment: Resveratrol,Nimbolide, Lovastatin,Bortezomib, Vorinostat,Berberine, Pterostilbene,Deguelin, Andrographolide,and Colchicine

Oral cancer is one of the main causes of cancer-related deaths in South-Asian countries. There are very limited treatment options available for oral cancer. Research endeavors focused on discovery and development of novel therapies for oral cancer, is necessary to control the ever rising oral cancer related mortalities. We mined the large pool of compounds from the publicly available compound databases, to identify potential therapeutic compounds for oral cancer. Over 84 million compounds were screened for the possible anti-cancer activity by custom build SVM classifier. The molecular targets of the predicted anti-cancer compounds were mined from reliable sources like experimental bioassays studies associated with the compound, and from protein-compound interaction databases. Therapeutic compounds from DrugBank, and a list of natural anti-cancer compounds derived from literature mining of published studies, were used for building partial least squares regression model. The regression model thus built, was used for the estimation of oral cancer specific weights based on the molecular targets. These weights were used to compute scores for screening the predicted anti-cancer compounds for their potential to treat oral cancer. The list of potential compounds was annotated with corresponding physicochemical properties, cancer specific bioactivity evidences, and literature evidences. In all, 288 compounds with the potential to treat oral cancer were identified in the current study. The majority of the compounds in this list are natural products, which are well-tolerated and have minimal side-effects compared to the synthetic counterparts. Some of the potential therapeutic compounds identified in the current study are resveratrol, nimbolide, lovastatin, bortezomib, vorinostat, berberine, pterostilbene, deguelin, andrographolide, and colchicine.     

New Coleoptera records from New Brunswick, Canada: Stenotrachelidae, Oedemeridae, Meloidae, Myceteridae, Boridae, Pythidae, Pyrochroidae, Anthicidae, and Aderidae

We report 19 new species records for the faunal list of Coleoptera in New Brunswick, Canada, six of which are new records for the Maritime provinces, and one of which is new Canadian record. We also provide the first recent records for five additional species in New Brunswick. One new species of Stenotrachelidae, Cephaloon ungulare LeConte, is added to the New Brunswick faunal list. Additional records are provided for Cephaloon lepturides Newman, as well the first recent record of Nematoplus collaris LeConte. Two species of Oedemeridae, Asclera puncticollis (Say) and Asclera ruficollis (Say), are newly reported for New Brunswick, and additional locality and bionomic data are provided for Calopus angustus LeConte and Ditylus caeruleus (Randall). The records of Ditylus caerulus are the first recent records for the province. Three species of Meloidae, Epicauta pestifera Werner, Lytta sayi LeConte, and Meloe augustcollis Say are reported the first time for New Brunswick; Epicauta pestifera is newly recorded in Canada. Lacconotus punctatus LeConte and the family Mycteridaeis newly recorded for New Brunswick. The first recent records of Borus unicolor Say (Boridae) are reported from the province. One new species of Pythidae, Pytho siedlitzi Blair, and the first recent records of Pytho niger Kirby are added to the faunal list of New Brunswick. Three species of Pyrochroidae are newly reported for the province, including Pedilus canaliculatus (LeConte) and Pedilus elegans (Hentz), which are new for the Maritime provinces. Five species of Anthicidae and the first recent record of Anthicus cervinus LaFerté-Sénectére are newly reported for New Brunswick. Anthicus melancholicus LaFerté-Sénectère, Sapintus pubescens (LaFerté-Sénectère), Notoxus bifasciatus (LeConte), and Stereopalpus rufipes Casey are new to the Maritime provinces faunal list. Ambyderus granularis (LeConte) is removed from the faunal list of the province. Three species of Aderidae, Vanonus huronicus Casey, Zonantes fasciatus (Melsheimer), and Zonantes pallidusWerner, are newly recorded for New Brunswick; Zonantes fasciatus and Vanonus huronicus are new for the Maritime provinces' faunal list. Collection data, bionomic data, and distribution maps are presented for all these species.